Experiment: acetate 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4202 and Pf1N1B4_4203 overlap by 4 nucleotides Pf1N1B4_4203 and Pf1N1B4_4204 are separated by 2 nucleotides
Pf1N1B4_4202 - 4-oxalmesaconate hydratase (EC 4.2.1.83), at 4,654,401 to 4,655,135
_4202
Pf1N1B4_4203 - Putative siderophore biosynthesis protein, related to 2-demethylmenaquinone methyltransferase, at 4,655,132 to 4,655,848
_4203
Pf1N1B4_4204 - FldA protein, at 4,655,851 to 4,656,939
_4204
Position (kb)
4655
4656
4657 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4654.855 kb on + strand at 4654.959 kb on + strand, within Pf1N1B4_4202 at 4655.004 kb on + strand, within Pf1N1B4_4202 at 4655.004 kb on + strand, within Pf1N1B4_4202 at 4655.007 kb on + strand, within Pf1N1B4_4202 at 4655.007 kb on + strand at 4655.048 kb on + strand, within Pf1N1B4_4202 at 4655.049 kb on - strand, within Pf1N1B4_4202 at 4655.082 kb on + strand at 4655.082 kb on + strand at 4655.095 kb on - strand at 4655.173 kb on + strand at 4655.174 kb on - strand at 4655.777 kb on + strand at 4655.777 kb on + strand at 4655.777 kb on + strand at 4655.777 kb on + strand at 4655.778 kb on - strand at 4655.909 kb on - strand at 4655.928 kb on - strand at 4655.960 kb on + strand, within Pf1N1B4_4204 at 4656.220 kb on + strand, within Pf1N1B4_4204 at 4656.220 kb on + strand, within Pf1N1B4_4204 at 4656.221 kb on - strand, within Pf1N1B4_4204 at 4656.221 kb on - strand, within Pf1N1B4_4204 at 4656.257 kb on + strand, within Pf1N1B4_4204 at 4656.377 kb on + strand, within Pf1N1B4_4204 at 4656.584 kb on + strand, within Pf1N1B4_4204 at 4656.584 kb on + strand, within Pf1N1B4_4204 at 4656.584 kb on + strand, within Pf1N1B4_4204 at 4656.585 kb on - strand, within Pf1N1B4_4204 at 4656.585 kb on - strand, within Pf1N1B4_4204 at 4656.632 kb on + strand, within Pf1N1B4_4204 at 4656.897 kb on - strand at 4656.978 kb on - strand at 4657.034 kb on - strand at 4657.078 kb on + strand at 4657.085 kb on + strand at 4657.183 kb on + strand at 4657.263 kb on - strand at 4657.263 kb on - strand at 4657.326 kb on + strand at 4657.327 kb on - strand at 4657.327 kb on - strand at 4657.332 kb on + strand at 4657.333 kb on - strand at 4657.389 kb on + strand at 4657.390 kb on - strand at 4657.390 kb on - strand at 4657.464 kb on + strand at 4657.680 kb on - strand at 4657.832 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction acetate 20 mM remove 4,654,855 + +0.6 4,654,959 + Pf1N1B4_4202 0.76 -1.1 4,655,004 + Pf1N1B4_4202 0.82 -0.6 4,655,004 + Pf1N1B4_4202 0.82 -1.2 4,655,007 + Pf1N1B4_4202 0.82 +0.4 4,655,007 + -0.4 4,655,048 + Pf1N1B4_4202 0.88 +0.2 4,655,049 - Pf1N1B4_4202 0.88 +0.4 4,655,082 + -1.1 4,655,082 + +0.6 4,655,095 - +0.0 4,655,173 + -0.4 4,655,174 - +0.6 4,655,777 + +0.0 4,655,777 + -1.4 4,655,777 + -0.4 4,655,777 + -1.7 4,655,778 - -1.0 4,655,909 - -0.4 4,655,928 - +0.0 4,655,960 + Pf1N1B4_4204 0.10 +0.3 4,656,220 + Pf1N1B4_4204 0.34 +2.2 4,656,220 + Pf1N1B4_4204 0.34 -1.0 4,656,221 - Pf1N1B4_4204 0.34 -2.4 4,656,221 - Pf1N1B4_4204 0.34 -1.0 4,656,257 + Pf1N1B4_4204 0.37 -0.7 4,656,377 + Pf1N1B4_4204 0.48 +0.6 4,656,584 + Pf1N1B4_4204 0.67 -1.0 4,656,584 + Pf1N1B4_4204 0.67 +0.6 4,656,584 + Pf1N1B4_4204 0.67 -1.7 4,656,585 - Pf1N1B4_4204 0.67 -1.1 4,656,585 - Pf1N1B4_4204 0.67 +1.0 4,656,632 + Pf1N1B4_4204 0.72 -0.5 4,656,897 - +0.2 4,656,978 - +0.6 4,657,034 - +0.0 4,657,078 + +0.6 4,657,085 + -1.0 4,657,183 + -0.4 4,657,263 - -1.0 4,657,263 - +2.2 4,657,326 + -0.4 4,657,327 - +2.6 4,657,327 - -0.7 4,657,332 + -0.0 4,657,333 - -1.7 4,657,389 + -0.3 4,657,390 - -0.1 4,657,390 - -0.1 4,657,464 + +0.0 4,657,680 - -1.7 4,657,832 - +0.3
Or see this region's nucleotide sequence