Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_4173

Experiment: acetate 20 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_4171 and Pf1N1B4_4172 are separated by 38 nucleotidesPf1N1B4_4172 and Pf1N1B4_4173 are separated by 55 nucleotidesPf1N1B4_4173 and Pf1N1B4_4174 are separated by 55 nucleotidesPf1N1B4_4174 and Pf1N1B4_4175 overlap by 15 nucleotides Pf1N1B4_4171 - sensor histidine kinase, at 4,620,911 to 4,622,731 _4171 Pf1N1B4_4172 - hypothetical protein, at 4,622,770 to 4,622,904 _4172 Pf1N1B4_4173 - Acetyl-CoA synthetase (ADP-forming) alpha and beta chains, putative, at 4,622,960 to 4,623,523 _4173 Pf1N1B4_4174 - hypothetical protein, at 4,623,579 to 4,623,761 _4174 Pf1N1B4_4175 - Universal stress protein family 5, at 4,623,747 to 4,624,667 _4175 Position (kb) 4622 4623 4624Strain fitness (log2 ratio) -2 -1 0 1 2 3at 4622.102 kb on + strand, within Pf1N1B4_4171at 4622.151 kb on + strand, within Pf1N1B4_4171at 4622.171 kb on - strand, within Pf1N1B4_4171at 4622.208 kb on + strand, within Pf1N1B4_4171at 4622.209 kb on - strand, within Pf1N1B4_4171at 4622.209 kb on - strand, within Pf1N1B4_4171at 4622.209 kb on - strand, within Pf1N1B4_4171at 4622.209 kb on - strand, within Pf1N1B4_4171at 4622.317 kb on + strand, within Pf1N1B4_4171at 4622.348 kb on + strand, within Pf1N1B4_4171at 4622.464 kb on + strand, within Pf1N1B4_4171at 4622.465 kb on - strand, within Pf1N1B4_4171at 4622.620 kb on - strandat 4622.641 kb on - strandat 4622.666 kb on + strandat 4622.666 kb on + strandat 4622.837 kb on + strand, within Pf1N1B4_4172at 4622.837 kb on + strand, within Pf1N1B4_4172at 4622.838 kb on - strand, within Pf1N1B4_4172at 4622.838 kb on - strand, within Pf1N1B4_4172at 4622.843 kb on + strand, within Pf1N1B4_4172at 4622.967 kb on + strandat 4622.967 kb on + strandat 4623.075 kb on + strand, within Pf1N1B4_4173at 4623.075 kb on + strand, within Pf1N1B4_4173at 4623.076 kb on - strand, within Pf1N1B4_4173at 4623.076 kb on - strand, within Pf1N1B4_4173at 4623.261 kb on + strand, within Pf1N1B4_4173at 4623.262 kb on - strand, within Pf1N1B4_4173at 4623.262 kb on - strand, within Pf1N1B4_4173at 4623.262 kb on - strand, within Pf1N1B4_4173at 4623.262 kb on - strand, within Pf1N1B4_4173at 4623.262 kb on - strand, within Pf1N1B4_4173at 4623.262 kb on - strand, within Pf1N1B4_4173at 4623.262 kb on - strand, within Pf1N1B4_4173at 4623.385 kb on - strand, within Pf1N1B4_4173at 4623.667 kb on - strand, within Pf1N1B4_4174at 4623.709 kb on + strand, within Pf1N1B4_4174at 4623.709 kb on + strand, within Pf1N1B4_4174at 4623.792 kb on + strandat 4623.868 kb on + strand, within Pf1N1B4_4175at 4623.868 kb on + strand, within Pf1N1B4_4175at 4623.868 kb on + strand, within Pf1N1B4_4175at 4623.868 kb on + strand, within Pf1N1B4_4175at 4623.869 kb on - strand, within Pf1N1B4_4175at 4623.869 kb on - strandat 4624.306 kb on + strandat 4624.329 kb on + strand, within Pf1N1B4_4175at 4624.329 kb on + strandat 4624.329 kb on + strand, within Pf1N1B4_4175at 4624.329 kb on + strand, within Pf1N1B4_4175at 4624.330 kb on - strand, within Pf1N1B4_4175

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Per-strain Table

Position Strand Gene LocusTag Fraction acetate 20 mM
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4,622,102 + Pf1N1B4_4171 0.65 -1.4
4,622,151 + Pf1N1B4_4171 0.68 +0.6
4,622,171 - Pf1N1B4_4171 0.69 +0.6
4,622,208 + Pf1N1B4_4171 0.71 -0.8
4,622,209 - Pf1N1B4_4171 0.71 +1.6
4,622,209 - Pf1N1B4_4171 0.71 +1.1
4,622,209 - Pf1N1B4_4171 0.71 +0.1
4,622,209 - Pf1N1B4_4171 0.71 +0.2
4,622,317 + Pf1N1B4_4171 0.77 +0.2
4,622,348 + Pf1N1B4_4171 0.79 -2.7
4,622,464 + Pf1N1B4_4171 0.85 +0.4
4,622,465 - Pf1N1B4_4171 0.85 -0.8
4,622,620 - -1.7
4,622,641 - -1.2
4,622,666 + +0.3
4,622,666 + -0.1
4,622,837 + Pf1N1B4_4172 0.50 -0.4
4,622,837 + Pf1N1B4_4172 0.50 +0.6
4,622,838 - Pf1N1B4_4172 0.50 +2.6
4,622,838 - Pf1N1B4_4172 0.50 -0.8
4,622,843 + Pf1N1B4_4172 0.54 -2.2
4,622,967 + -0.4
4,622,967 + -0.6
4,623,075 + Pf1N1B4_4173 0.20 -1.7
4,623,075 + Pf1N1B4_4173 0.20 +0.0
4,623,076 - Pf1N1B4_4173 0.21 -2.0
4,623,076 - Pf1N1B4_4173 0.21 -2.4
4,623,261 + Pf1N1B4_4173 0.53 -1.7
4,623,262 - Pf1N1B4_4173 0.54 -0.7
4,623,262 - Pf1N1B4_4173 0.54 -0.4
4,623,262 - Pf1N1B4_4173 0.54 -1.0
4,623,262 - Pf1N1B4_4173 0.54 -0.4
4,623,262 - Pf1N1B4_4173 0.54 -0.4
4,623,262 - Pf1N1B4_4173 0.54 -1.0
4,623,262 - Pf1N1B4_4173 0.54 +2.8
4,623,385 - Pf1N1B4_4173 0.75 +0.1
4,623,667 - Pf1N1B4_4174 0.48 -0.4
4,623,709 + Pf1N1B4_4174 0.71 -1.5
4,623,709 + Pf1N1B4_4174 0.71 +1.2
4,623,792 + -1.4
4,623,868 + Pf1N1B4_4175 0.13 -1.0
4,623,868 + Pf1N1B4_4175 0.13 -0.2
4,623,868 + Pf1N1B4_4175 0.13 -2.0
4,623,868 + Pf1N1B4_4175 0.13 -1.4
4,623,869 - Pf1N1B4_4175 0.13 -2.4
4,623,869 - +0.0
4,624,306 + -1.0
4,624,329 + Pf1N1B4_4175 0.63 +0.0
4,624,329 + +0.0
4,624,329 + Pf1N1B4_4175 0.63 +1.0
4,624,329 + Pf1N1B4_4175 0.63 -0.7
4,624,330 - Pf1N1B4_4175 0.63 -0.5

Or see this region's nucleotide sequence