Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_4017

Experiment: acetate 20 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_4016 and Pf1N1B4_4017 overlap by 4 nucleotidesPf1N1B4_4017 and Pf1N1B4_4018 are separated by 467 nucleotides Pf1N1B4_4016 - Heavy metal sensor histidine kinase, at 4,466,486 to 4,467,889 _4016 Pf1N1B4_4017 - DNA-binding heavy metal response regulator, at 4,467,886 to 4,468,560 _4017 Pf1N1B4_4018 - Heavy metal RND efflux outer membrane protein, CzcC family, at 4,469,028 to 4,470,287 _4018 Position (kb) 4467 4468 4469Strain fitness (log2 ratio) -2 -1 0 1at 4466.979 kb on - strandat 4467.052 kb on - strandat 4467.614 kb on + strand, within Pf1N1B4_4016at 4467.615 kb on - strand, within Pf1N1B4_4016at 4467.630 kb on + strand, within Pf1N1B4_4016at 4467.802 kb on - strandat 4467.845 kb on - strandat 4467.908 kb on + strandat 4468.272 kb on - strand, within Pf1N1B4_4017at 4468.272 kb on - strand, within Pf1N1B4_4017at 4468.415 kb on - strand, within Pf1N1B4_4017at 4468.427 kb on + strand, within Pf1N1B4_4017at 4468.428 kb on - strand, within Pf1N1B4_4017at 4468.486 kb on - strand, within Pf1N1B4_4017at 4468.513 kb on + strandat 4468.514 kb on - strandat 4468.514 kb on - strandat 4468.593 kb on - strandat 4468.823 kb on + strandat 4468.824 kb on - strandat 4468.849 kb on + strandat 4468.854 kb on - strandat 4468.874 kb on + strandat 4468.875 kb on - strandat 4468.875 kb on - strandat 4468.941 kb on + strandat 4469.038 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction acetate 20 mM
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4,466,979 - +0.7
4,467,052 - -0.3
4,467,614 + Pf1N1B4_4016 0.80 -1.9
4,467,615 - Pf1N1B4_4016 0.80 -0.1
4,467,630 + Pf1N1B4_4016 0.81 +0.9
4,467,802 - +1.4
4,467,845 - +0.7
4,467,908 + +0.1
4,468,272 - Pf1N1B4_4017 0.57 -0.9
4,468,272 - Pf1N1B4_4017 0.57 -1.3
4,468,415 - Pf1N1B4_4017 0.78 -1.3
4,468,427 + Pf1N1B4_4017 0.80 +0.1
4,468,428 - Pf1N1B4_4017 0.80 -1.3
4,468,486 - Pf1N1B4_4017 0.89 -1.1
4,468,513 + +0.9
4,468,514 - +1.7
4,468,514 - -0.9
4,468,593 - -0.9
4,468,823 + +0.1
4,468,824 - -0.1
4,468,849 + -0.3
4,468,854 - +0.7
4,468,874 + -0.9
4,468,875 - -0.9
4,468,875 - -0.8
4,468,941 + +0.3
4,469,038 + -0.6

Or see this region's nucleotide sequence