Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_3713

Experiment: acetate 20 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_3711 and Pf1N1B4_3712 are separated by 146 nucleotidesPf1N1B4_3712 and Pf1N1B4_3713 overlap by 4 nucleotidesPf1N1B4_3713 and Pf1N1B4_3714 overlap by 4 nucleotidesPf1N1B4_3714 and Pf1N1B4_3715 are separated by 2 nucleotides Pf1N1B4_3711 - Cobyric acid synthase (EC 6.3.5.10), at 4,119,580 to 4,121,073 _3711 Pf1N1B4_3712 - Adenosylcobinamide-phosphate guanylyltransferase (EC 2.7.7.62), at 4,121,220 to 4,121,753 _3712 Pf1N1B4_3713 - Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase (EC 2.4.2.21), at 4,121,750 to 4,122,805 _3713 Pf1N1B4_3714 - Alpha-ribazole-5'-phosphate phosphatase (EC 3.1.3.73), at 4,122,802 to 4,123,377 _3714 Pf1N1B4_3715 - Cobalamin synthase, at 4,123,380 to 4,124,111 _3715 Position (kb) 4121 4122 4123Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 4120.894 kb on + strand, within Pf1N1B4_3711at 4120.894 kb on + strand, within Pf1N1B4_3711at 4120.894 kb on + strand, within Pf1N1B4_3711at 4120.894 kb on + strand, within Pf1N1B4_3711at 4120.894 kb on + strand, within Pf1N1B4_3711at 4120.894 kb on + strand, within Pf1N1B4_3711at 4120.895 kb on - strand, within Pf1N1B4_3711at 4121.307 kb on + strand, within Pf1N1B4_3712at 4121.307 kb on + strand, within Pf1N1B4_3712at 4121.307 kb on + strand, within Pf1N1B4_3712at 4121.307 kb on + strand, within Pf1N1B4_3712at 4121.307 kb on + strand, within Pf1N1B4_3712at 4121.307 kb on + strandat 4121.307 kb on + strand, within Pf1N1B4_3712at 4121.307 kb on + strand, within Pf1N1B4_3712at 4121.307 kb on + strand, within Pf1N1B4_3712at 4121.307 kb on + strand, within Pf1N1B4_3712at 4121.307 kb on + strand, within Pf1N1B4_3712at 4121.307 kb on + strand, within Pf1N1B4_3712at 4121.308 kb on - strandat 4121.308 kb on - strand, within Pf1N1B4_3712at 4121.308 kb on - strand, within Pf1N1B4_3712at 4121.308 kb on - strand, within Pf1N1B4_3712at 4121.308 kb on - strand, within Pf1N1B4_3712at 4121.308 kb on - strand, within Pf1N1B4_3712at 4121.308 kb on - strand, within Pf1N1B4_3712at 4121.308 kb on - strand, within Pf1N1B4_3712at 4121.308 kb on - strand, within Pf1N1B4_3712at 4121.308 kb on - strand, within Pf1N1B4_3712at 4121.308 kb on - strand, within Pf1N1B4_3712at 4121.308 kb on - strand, within Pf1N1B4_3712at 4121.308 kb on - strand, within Pf1N1B4_3712at 4121.308 kb on - strand, within Pf1N1B4_3712at 4121.308 kb on - strand, within Pf1N1B4_3712at 4121.308 kb on - strand, within Pf1N1B4_3712at 4121.308 kb on - strand, within Pf1N1B4_3712at 4121.308 kb on - strand, within Pf1N1B4_3712at 4121.309 kb on + strand, within Pf1N1B4_3712at 4121.309 kb on + strand, within Pf1N1B4_3712at 4121.309 kb on + strand, within Pf1N1B4_3712at 4121.309 kb on + strand, within Pf1N1B4_3712at 4121.309 kb on + strand, within Pf1N1B4_3712at 4121.309 kb on + strand, within Pf1N1B4_3712at 4121.310 kb on - strandat 4121.310 kb on - strand, within Pf1N1B4_3712at 4121.310 kb on - strand, within Pf1N1B4_3712at 4121.310 kb on - strand, within Pf1N1B4_3712at 4121.310 kb on - strand, within Pf1N1B4_3712at 4121.310 kb on - strand, within Pf1N1B4_3712at 4121.310 kb on - strand, within Pf1N1B4_3712at 4121.311 kb on + strand, within Pf1N1B4_3712at 4121.311 kb on + strand, within Pf1N1B4_3712at 4121.311 kb on + strand, within Pf1N1B4_3712at 4121.311 kb on + strandat 4121.311 kb on + strand, within Pf1N1B4_3712at 4121.319 kb on + strand, within Pf1N1B4_3712at 4121.319 kb on + strandat 4121.319 kb on + strand, within Pf1N1B4_3712at 4121.319 kb on + strand, within Pf1N1B4_3712at 4121.319 kb on + strand, within Pf1N1B4_3712at 4121.319 kb on + strandat 4121.320 kb on - strand, within Pf1N1B4_3712at 4121.320 kb on - strand, within Pf1N1B4_3712at 4121.320 kb on - strand, within Pf1N1B4_3712at 4121.320 kb on - strandat 4121.320 kb on - strand, within Pf1N1B4_3712at 4121.320 kb on - strand, within Pf1N1B4_3712at 4121.320 kb on - strandat 4121.646 kb on + strand, within Pf1N1B4_3712at 4121.646 kb on + strand, within Pf1N1B4_3712at 4121.832 kb on + strandat 4121.832 kb on + strandat 4121.832 kb on + strandat 4122.225 kb on + strand, within Pf1N1B4_3713at 4122.225 kb on + strandat 4122.225 kb on + strand, within Pf1N1B4_3713at 4122.226 kb on - strand, within Pf1N1B4_3713at 4122.226 kb on - strand, within Pf1N1B4_3713at 4122.433 kb on + strand, within Pf1N1B4_3713at 4122.544 kb on + strand, within Pf1N1B4_3713at 4122.545 kb on - strandat 4123.126 kb on + strand, within Pf1N1B4_3714at 4123.126 kb on + strand, within Pf1N1B4_3714at 4123.211 kb on - strand, within Pf1N1B4_3714at 4123.736 kb on - strandat 4123.736 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction acetate 20 mM
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4,120,894 + Pf1N1B4_3711 0.88 +0.6
4,120,894 + Pf1N1B4_3711 0.88 +0.4
4,120,894 + Pf1N1B4_3711 0.88 -2.1
4,120,894 + Pf1N1B4_3711 0.88 +0.0
4,120,894 + Pf1N1B4_3711 0.88 -1.7
4,120,894 + Pf1N1B4_3711 0.88 +1.3
4,120,895 - Pf1N1B4_3711 0.88 +1.6
4,121,307 + Pf1N1B4_3712 0.16 -0.4
4,121,307 + Pf1N1B4_3712 0.16 -1.0
4,121,307 + Pf1N1B4_3712 0.16 -0.2
4,121,307 + Pf1N1B4_3712 0.16 -0.2
4,121,307 + Pf1N1B4_3712 0.16 -0.3
4,121,307 + +0.8
4,121,307 + Pf1N1B4_3712 0.16 -0.6
4,121,307 + Pf1N1B4_3712 0.16 -1.2
4,121,307 + Pf1N1B4_3712 0.16 -0.0
4,121,307 + Pf1N1B4_3712 0.16 -0.2
4,121,307 + Pf1N1B4_3712 0.16 -0.4
4,121,307 + Pf1N1B4_3712 0.16 -0.3
4,121,308 - -0.4
4,121,308 - Pf1N1B4_3712 0.16 -0.2
4,121,308 - Pf1N1B4_3712 0.16 -1.7
4,121,308 - Pf1N1B4_3712 0.16 +1.6
4,121,308 - Pf1N1B4_3712 0.16 +0.2
4,121,308 - Pf1N1B4_3712 0.16 -0.5
4,121,308 - Pf1N1B4_3712 0.16 -0.3
4,121,308 - Pf1N1B4_3712 0.16 +0.1
4,121,308 - Pf1N1B4_3712 0.16 +1.3
4,121,308 - Pf1N1B4_3712 0.16 -0.7
4,121,308 - Pf1N1B4_3712 0.16 +0.8
4,121,308 - Pf1N1B4_3712 0.16 +0.6
4,121,308 - Pf1N1B4_3712 0.16 +0.4
4,121,308 - Pf1N1B4_3712 0.16 -0.3
4,121,308 - Pf1N1B4_3712 0.16 +0.0
4,121,308 - Pf1N1B4_3712 0.16 +0.5
4,121,308 - Pf1N1B4_3712 0.16 +1.1
4,121,308 - Pf1N1B4_3712 0.16 +2.6
4,121,309 + Pf1N1B4_3712 0.17 -1.1
4,121,309 + Pf1N1B4_3712 0.17 +0.1
4,121,309 + Pf1N1B4_3712 0.17 -1.2
4,121,309 + Pf1N1B4_3712 0.17 -0.2
4,121,309 + Pf1N1B4_3712 0.17 -0.2
4,121,309 + Pf1N1B4_3712 0.17 +0.8
4,121,310 - +0.3
4,121,310 - Pf1N1B4_3712 0.17 -0.2
4,121,310 - Pf1N1B4_3712 0.17 -0.7
4,121,310 - Pf1N1B4_3712 0.17 +0.2
4,121,310 - Pf1N1B4_3712 0.17 +0.3
4,121,310 - Pf1N1B4_3712 0.17 +2.1
4,121,310 - Pf1N1B4_3712 0.17 -1.6
4,121,311 + Pf1N1B4_3712 0.17 -0.1
4,121,311 + Pf1N1B4_3712 0.17 -0.4
4,121,311 + Pf1N1B4_3712 0.17 +0.1
4,121,311 + +0.8
4,121,311 + Pf1N1B4_3712 0.17 -1.1
4,121,319 + Pf1N1B4_3712 0.19 +0.4
4,121,319 + +0.8
4,121,319 + Pf1N1B4_3712 0.19 -3.0
4,121,319 + Pf1N1B4_3712 0.19 +0.4
4,121,319 + Pf1N1B4_3712 0.19 -0.7
4,121,319 + +2.4
4,121,320 - Pf1N1B4_3712 0.19 +0.6
4,121,320 - Pf1N1B4_3712 0.19 -0.2
4,121,320 - Pf1N1B4_3712 0.19 -0.7
4,121,320 - +1.8
4,121,320 - Pf1N1B4_3712 0.19 +2.3
4,121,320 - Pf1N1B4_3712 0.19 -0.3
4,121,320 - +1.4
4,121,646 + Pf1N1B4_3712 0.80 +0.8
4,121,646 + Pf1N1B4_3712 0.80 -0.2
4,121,832 + +0.3
4,121,832 + +0.1
4,121,832 + +0.3
4,122,225 + Pf1N1B4_3713 0.45 -1.5
4,122,225 + -0.2
4,122,225 + Pf1N1B4_3713 0.45 +1.4
4,122,226 - Pf1N1B4_3713 0.45 +1.4
4,122,226 - Pf1N1B4_3713 0.45 -0.5
4,122,433 + Pf1N1B4_3713 0.65 -1.5
4,122,544 + Pf1N1B4_3713 0.75 -0.5
4,122,545 - +0.2
4,123,126 + Pf1N1B4_3714 0.56 -1.3
4,123,126 + Pf1N1B4_3714 0.56 +1.4
4,123,211 - Pf1N1B4_3714 0.71 +1.6
4,123,736 - -1.2
4,123,736 - +1.8

Or see this region's nucleotide sequence