Experiment: acetate 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3487 and Pf1N1B4_3488 are separated by 145 nucleotides Pf1N1B4_3488 and Pf1N1B4_3489 are separated by 136 nucleotides Pf1N1B4_3489 and Pf1N1B4_3490 are separated by 158 nucleotides
Pf1N1B4_3487 - Aerobic C4-dicarboxylate transporter for fumarate, L-malate, D-malate, succunate, aspartate, at 3,894,365 to 3,895,699
_3487
Pf1N1B4_3488 - Alpha-methylacyl-CoA racemase (EC 5.1.99.4), at 3,895,845 to 3,897,026
_3488
Pf1N1B4_3489 - Protein sprT, at 3,897,163 to 3,897,657
_3489
Pf1N1B4_3490 - probable membrane protein YPO2362, at 3,897,816 to 3,898,418
_3490
Position (kb)
3895
3896
3897
3898 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3895.001 kb on + strand, within Pf1N1B4_3487 at 3895.049 kb on + strand, within Pf1N1B4_3487 at 3895.049 kb on + strand, within Pf1N1B4_3487 at 3895.049 kb on + strand, within Pf1N1B4_3487 at 3895.049 kb on + strand, within Pf1N1B4_3487 at 3895.049 kb on + strand, within Pf1N1B4_3487 at 3895.049 kb on + strand, within Pf1N1B4_3487 at 3895.049 kb on + strand, within Pf1N1B4_3487 at 3895.049 kb on + strand, within Pf1N1B4_3487 at 3895.049 kb on + strand, within Pf1N1B4_3487 at 3895.049 kb on + strand, within Pf1N1B4_3487 at 3895.049 kb on + strand, within Pf1N1B4_3487 at 3895.049 kb on + strand, within Pf1N1B4_3487 at 3895.049 kb on + strand, within Pf1N1B4_3487 at 3895.049 kb on + strand, within Pf1N1B4_3487 at 3895.049 kb on + strand, within Pf1N1B4_3487 at 3895.049 kb on + strand, within Pf1N1B4_3487 at 3895.050 kb on - strand at 3895.050 kb on - strand, within Pf1N1B4_3487 at 3895.050 kb on - strand, within Pf1N1B4_3487 at 3895.050 kb on - strand, within Pf1N1B4_3487 at 3895.133 kb on + strand at 3895.283 kb on - strand, within Pf1N1B4_3487 at 3895.283 kb on - strand, within Pf1N1B4_3487 at 3895.292 kb on + strand, within Pf1N1B4_3487 at 3895.292 kb on + strand, within Pf1N1B4_3487 at 3895.293 kb on - strand, within Pf1N1B4_3487 at 3895.293 kb on - strand, within Pf1N1B4_3487 at 3895.333 kb on + strand, within Pf1N1B4_3487 at 3895.334 kb on - strand, within Pf1N1B4_3487 at 3895.334 kb on - strand, within Pf1N1B4_3487 at 3895.650 kb on + strand at 3895.651 kb on - strand at 3895.697 kb on + strand at 3895.697 kb on + strand at 3895.697 kb on + strand at 3895.697 kb on + strand at 3895.697 kb on + strand at 3895.697 kb on + strand at 3895.697 kb on + strand at 3895.697 kb on + strand at 3895.697 kb on + strand at 3895.697 kb on + strand at 3895.697 kb on + strand at 3895.698 kb on - strand at 3895.698 kb on - strand at 3895.698 kb on - strand at 3895.698 kb on - strand at 3895.698 kb on - strand at 3895.698 kb on - strand at 3895.701 kb on + strand at 3895.702 kb on - strand at 3895.702 kb on - strand at 3895.814 kb on + strand at 3895.844 kb on + strand at 3895.844 kb on + strand at 3895.844 kb on + strand at 3895.845 kb on - strand at 3895.845 kb on - strand at 3895.845 kb on - strand at 3895.845 kb on - strand at 3895.845 kb on - strand at 3895.845 kb on - strand at 3895.845 kb on - strand at 3895.845 kb on - strand at 3895.845 kb on - strand at 3895.845 kb on - strand at 3895.845 kb on - strand at 3895.845 kb on - strand at 3895.845 kb on - strand at 3895.845 kb on - strand at 3895.845 kb on - strand at 3896.025 kb on + strand at 3896.025 kb on + strand, within Pf1N1B4_3488 at 3896.025 kb on + strand, within Pf1N1B4_3488 at 3896.202 kb on + strand, within Pf1N1B4_3488 at 3896.202 kb on + strand, within Pf1N1B4_3488 at 3896.202 kb on + strand, within Pf1N1B4_3488 at 3896.202 kb on + strand, within Pf1N1B4_3488 at 3896.202 kb on + strand, within Pf1N1B4_3488 at 3896.202 kb on + strand at 3896.202 kb on + strand, within Pf1N1B4_3488 at 3896.202 kb on + strand, within Pf1N1B4_3488 at 3896.202 kb on + strand, within Pf1N1B4_3488 at 3896.203 kb on - strand, within Pf1N1B4_3488 at 3896.203 kb on - strand, within Pf1N1B4_3488 at 3896.203 kb on - strand, within Pf1N1B4_3488 at 3896.238 kb on + strand, within Pf1N1B4_3488 at 3896.238 kb on + strand, within Pf1N1B4_3488 at 3896.239 kb on - strand, within Pf1N1B4_3488 at 3896.239 kb on - strand, within Pf1N1B4_3488 at 3896.268 kb on + strand, within Pf1N1B4_3488 at 3896.269 kb on - strand, within Pf1N1B4_3488 at 3896.269 kb on - strand, within Pf1N1B4_3488 at 3896.295 kb on + strand, within Pf1N1B4_3488 at 3896.296 kb on - strand, within Pf1N1B4_3488 at 3896.548 kb on - strand, within Pf1N1B4_3488 at 3896.548 kb on - strand, within Pf1N1B4_3488 at 3896.548 kb on - strand, within Pf1N1B4_3488 at 3896.553 kb on + strand, within Pf1N1B4_3488 at 3896.553 kb on + strand, within Pf1N1B4_3488 at 3896.553 kb on + strand at 3896.554 kb on - strand, within Pf1N1B4_3488 at 3896.556 kb on + strand, within Pf1N1B4_3488 at 3896.556 kb on + strand, within Pf1N1B4_3488 at 3896.724 kb on + strand, within Pf1N1B4_3488 at 3896.785 kb on + strand at 3896.785 kb on + strand, within Pf1N1B4_3488 at 3896.785 kb on + strand, within Pf1N1B4_3488 at 3896.785 kb on + strand, within Pf1N1B4_3488 at 3896.785 kb on + strand, within Pf1N1B4_3488 at 3896.786 kb on - strand, within Pf1N1B4_3488 at 3896.786 kb on - strand, within Pf1N1B4_3488 at 3896.786 kb on - strand at 3897.001 kb on + strand at 3897.020 kb on + strand at 3897.020 kb on + strand at 3897.020 kb on + strand at 3897.082 kb on + strand at 3897.165 kb on - strand at 3897.165 kb on - strand at 3897.165 kb on - strand at 3897.296 kb on + strand, within Pf1N1B4_3489 at 3897.296 kb on + strand, within Pf1N1B4_3489 at 3897.297 kb on - strand, within Pf1N1B4_3489 at 3897.297 kb on - strand, within Pf1N1B4_3489 at 3897.297 kb on - strand, within Pf1N1B4_3489 at 3897.297 kb on - strand, within Pf1N1B4_3489 at 3897.297 kb on - strand, within Pf1N1B4_3489 at 3897.297 kb on - strand, within Pf1N1B4_3489 at 3897.303 kb on - strand, within Pf1N1B4_3489 at 3897.303 kb on - strand, within Pf1N1B4_3489 at 3897.332 kb on + strand, within Pf1N1B4_3489 at 3897.332 kb on + strand, within Pf1N1B4_3489 at 3897.332 kb on + strand, within Pf1N1B4_3489 at 3897.333 kb on - strand, within Pf1N1B4_3489 at 3897.333 kb on - strand at 3897.333 kb on - strand, within Pf1N1B4_3489 at 3897.362 kb on + strand, within Pf1N1B4_3489 at 3897.758 kb on + strand at 3897.758 kb on + strand at 3897.758 kb on + strand at 3897.758 kb on + strand at 3897.817 kb on + strand at 3897.817 kb on + strand at 3897.818 kb on - strand at 3897.818 kb on - strand at 3897.818 kb on - strand at 3897.818 kb on - strand at 3897.818 kb on - strand at 3897.818 kb on - strand at 3897.825 kb on - strand at 3897.826 kb on + strand at 3897.970 kb on + strand, within Pf1N1B4_3490 at 3897.970 kb on + strand, within Pf1N1B4_3490 at 3897.970 kb on + strand, within Pf1N1B4_3490 at 3897.970 kb on + strand, within Pf1N1B4_3490 at 3897.970 kb on + strand, within Pf1N1B4_3490 at 3897.970 kb on + strand, within Pf1N1B4_3490 at 3897.970 kb on + strand, within Pf1N1B4_3490 at 3897.970 kb on + strand at 3897.971 kb on - strand, within Pf1N1B4_3490 at 3897.971 kb on - strand, within Pf1N1B4_3490 at 3897.971 kb on - strand, within Pf1N1B4_3490 at 3897.971 kb on - strand, within Pf1N1B4_3490 at 3897.979 kb on + strand, within Pf1N1B4_3490 at 3897.979 kb on + strand, within Pf1N1B4_3490 at 3897.979 kb on + strand at 3897.980 kb on - strand, within Pf1N1B4_3490 at 3897.989 kb on - strand, within Pf1N1B4_3490 at 3897.989 kb on - strand, within Pf1N1B4_3490
Per-strain Table
Position Strand Gene LocusTag Fraction acetate 20 mM remove 3,895,001 + Pf1N1B4_3487 0.48 -1.9 3,895,049 + Pf1N1B4_3487 0.51 -2.1 3,895,049 + Pf1N1B4_3487 0.51 -0.3 3,895,049 + Pf1N1B4_3487 0.51 -1.0 3,895,049 + Pf1N1B4_3487 0.51 +1.0 3,895,049 + Pf1N1B4_3487 0.51 +1.0 3,895,049 + Pf1N1B4_3487 0.51 +0.9 3,895,049 + Pf1N1B4_3487 0.51 +0.9 3,895,049 + Pf1N1B4_3487 0.51 +0.0 3,895,049 + Pf1N1B4_3487 0.51 -0.7 3,895,049 + Pf1N1B4_3487 0.51 -0.2 3,895,049 + Pf1N1B4_3487 0.51 +0.9 3,895,049 + Pf1N1B4_3487 0.51 +1.2 3,895,049 + Pf1N1B4_3487 0.51 +1.2 3,895,049 + Pf1N1B4_3487 0.51 -0.4 3,895,049 + Pf1N1B4_3487 0.51 +0.3 3,895,049 + Pf1N1B4_3487 0.51 +1.6 3,895,050 - -1.1 3,895,050 - Pf1N1B4_3487 0.51 +1.1 3,895,050 - Pf1N1B4_3487 0.51 -0.4 3,895,050 - Pf1N1B4_3487 0.51 +3.5 3,895,133 + -0.1 3,895,283 - Pf1N1B4_3487 0.69 +0.5 3,895,283 - Pf1N1B4_3487 0.69 -1.1 3,895,292 + Pf1N1B4_3487 0.69 +1.9 3,895,292 + Pf1N1B4_3487 0.69 -0.1 3,895,293 - Pf1N1B4_3487 0.70 +0.9 3,895,293 - Pf1N1B4_3487 0.70 -1.1 3,895,333 + Pf1N1B4_3487 0.73 +0.5 3,895,334 - Pf1N1B4_3487 0.73 -0.7 3,895,334 - Pf1N1B4_3487 0.73 -0.9 3,895,650 + +1.9 3,895,651 - +1.9 3,895,697 + -0.7 3,895,697 + +0.7 3,895,697 + +0.5 3,895,697 + +0.3 3,895,697 + -1.7 3,895,697 + +0.5 3,895,697 + -1.4 3,895,697 + -0.2 3,895,697 + -1.2 3,895,697 + +0.1 3,895,697 + -0.1 3,895,698 - -1.1 3,895,698 - +0.7 3,895,698 - +0.2 3,895,698 - +0.2 3,895,698 - -2.1 3,895,698 - -0.1 3,895,701 + -0.1 3,895,702 - +0.6 3,895,702 - -0.7 3,895,814 + +0.2 3,895,844 + -1.3 3,895,844 + +0.9 3,895,844 + -1.7 3,895,845 - -1.4 3,895,845 - +1.6 3,895,845 - -0.6 3,895,845 - -1.7 3,895,845 - +0.9 3,895,845 - +0.5 3,895,845 - -0.7 3,895,845 - -0.7 3,895,845 - +0.5 3,895,845 - +0.9 3,895,845 - -0.1 3,895,845 - +0.9 3,895,845 - +0.5 3,895,845 - +0.2 3,895,845 - -0.4 3,896,025 + +1.9 3,896,025 + Pf1N1B4_3488 0.15 +1.1 3,896,025 + Pf1N1B4_3488 0.15 +0.5 3,896,202 + Pf1N1B4_3488 0.30 -1.2 3,896,202 + Pf1N1B4_3488 0.30 -0.4 3,896,202 + Pf1N1B4_3488 0.30 +1.2 3,896,202 + Pf1N1B4_3488 0.30 +0.9 3,896,202 + Pf1N1B4_3488 0.30 -0.4 3,896,202 + +1.5 3,896,202 + Pf1N1B4_3488 0.30 +1.7 3,896,202 + Pf1N1B4_3488 0.30 +0.5 3,896,202 + Pf1N1B4_3488 0.30 -1.1 3,896,203 - Pf1N1B4_3488 0.30 -1.1 3,896,203 - Pf1N1B4_3488 0.30 +0.9 3,896,203 - Pf1N1B4_3488 0.30 +0.5 3,896,238 + Pf1N1B4_3488 0.33 +1.1 3,896,238 + Pf1N1B4_3488 0.33 -0.7 3,896,239 - Pf1N1B4_3488 0.33 -0.3 3,896,239 - Pf1N1B4_3488 0.33 +0.6 3,896,268 + Pf1N1B4_3488 0.36 +0.4 3,896,269 - Pf1N1B4_3488 0.36 -0.1 3,896,269 - Pf1N1B4_3488 0.36 -0.4 3,896,295 + Pf1N1B4_3488 0.38 -0.1 3,896,296 - Pf1N1B4_3488 0.38 -1.1 3,896,548 - Pf1N1B4_3488 0.59 -0.7 3,896,548 - Pf1N1B4_3488 0.59 -0.3 3,896,548 - Pf1N1B4_3488 0.59 -1.9 3,896,553 + Pf1N1B4_3488 0.60 +1.6 3,896,553 + Pf1N1B4_3488 0.60 -2.3 3,896,553 + +0.9 3,896,554 - Pf1N1B4_3488 0.60 -0.5 3,896,556 + Pf1N1B4_3488 0.60 -1.7 3,896,556 + Pf1N1B4_3488 0.60 +0.5 3,896,724 + Pf1N1B4_3488 0.74 -0.7 3,896,785 + +0.9 3,896,785 + Pf1N1B4_3488 0.80 +0.1 3,896,785 + Pf1N1B4_3488 0.80 -1.1 3,896,785 + Pf1N1B4_3488 0.80 +0.6 3,896,785 + Pf1N1B4_3488 0.80 +0.4 3,896,786 - Pf1N1B4_3488 0.80 -0.3 3,896,786 - Pf1N1B4_3488 0.80 +0.4 3,896,786 - +3.2 3,897,001 + +1.5 3,897,020 + +1.2 3,897,020 + +0.1 3,897,020 + -2.1 3,897,082 + -0.2 3,897,165 - +0.7 3,897,165 - +0.2 3,897,165 - -1.9 3,897,296 + Pf1N1B4_3489 0.27 +0.9 3,897,296 + Pf1N1B4_3489 0.27 +0.5 3,897,297 - Pf1N1B4_3489 0.27 +0.6 3,897,297 - Pf1N1B4_3489 0.27 +0.9 3,897,297 - Pf1N1B4_3489 0.27 -0.7 3,897,297 - Pf1N1B4_3489 0.27 +1.9 3,897,297 - Pf1N1B4_3489 0.27 +1.2 3,897,297 - Pf1N1B4_3489 0.27 -0.7 3,897,303 - Pf1N1B4_3489 0.28 +0.9 3,897,303 - Pf1N1B4_3489 0.28 -0.4 3,897,332 + Pf1N1B4_3489 0.34 +1.3 3,897,332 + Pf1N1B4_3489 0.34 -0.3 3,897,332 + Pf1N1B4_3489 0.34 -1.7 3,897,333 - Pf1N1B4_3489 0.34 -1.2 3,897,333 - +0.9 3,897,333 - Pf1N1B4_3489 0.34 +0.3 3,897,362 + Pf1N1B4_3489 0.40 -1.1 3,897,758 + +1.5 3,897,758 + +1.8 3,897,758 + +1.2 3,897,758 + -0.1 3,897,817 + -0.4 3,897,817 + -2.9 3,897,818 - -0.7 3,897,818 - -0.2 3,897,818 - -1.1 3,897,818 - -0.2 3,897,818 - -0.7 3,897,818 - -0.1 3,897,825 - -0.1 3,897,826 + -0.4 3,897,970 + Pf1N1B4_3490 0.26 -1.4 3,897,970 + Pf1N1B4_3490 0.26 +0.5 3,897,970 + Pf1N1B4_3490 0.26 -0.6 3,897,970 + Pf1N1B4_3490 0.26 -0.4 3,897,970 + Pf1N1B4_3490 0.26 -1.3 3,897,970 + Pf1N1B4_3490 0.26 -0.1 3,897,970 + Pf1N1B4_3490 0.26 +0.2 3,897,970 + +1.9 3,897,971 - Pf1N1B4_3490 0.26 +0.8 3,897,971 - Pf1N1B4_3490 0.26 +0.5 3,897,971 - Pf1N1B4_3490 0.26 +0.8 3,897,971 - Pf1N1B4_3490 0.26 -0.9 3,897,979 + Pf1N1B4_3490 0.27 +0.5 3,897,979 + Pf1N1B4_3490 0.27 +0.2 3,897,979 + -1.1 3,897,980 - Pf1N1B4_3490 0.27 +0.2 3,897,989 - Pf1N1B4_3490 0.29 +0.2 3,897,989 - Pf1N1B4_3490 0.29 -1.1
Or see this region's nucleotide sequence