Experiment: acetate 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_338 and Pf1N1B4_339 are separated by 359 nucleotides Pf1N1B4_339 and Pf1N1B4_340 overlap by 4 nucleotides Pf1N1B4_340 and Pf1N1B4_341 are separated by 56 nucleotides
Pf1N1B4_338 - Alpha-L-Rha alpha-1,3-L-rhamnosyltransferase (EC 2.4.1.-), at 401,787 to 402,794
_338
Pf1N1B4_339 - UDP-glucose 4-epimerase (EC 5.1.3.2), at 403,154 to 404,116
_339
Pf1N1B4_340 - Alpha-L-Rha alpha-1,3-L-rhamnosyltransferase (EC 2.4.1.-), at 404,113 to 405,036
_340
Pf1N1B4_341 - Glucose-1-phosphate thymidylyltransferase (EC 2.7.7.24), at 405,093 to 405,983
_341
Position (kb) 403
404
405 Strain fitness (log2 ratio) -2
-1
0
1
2 at 402.198 kb on + strand, within Pf1N1B4_338 at 402.443 kb on - strand, within Pf1N1B4_338 at 402.474 kb on - strand at 402.554 kb on - strand, within Pf1N1B4_338 at 402.881 kb on - strand at 402.930 kb on - strand at 403.004 kb on - strand at 403.016 kb on + strand at 403.017 kb on - strand at 403.017 kb on - strand at 403.017 kb on - strand at 403.017 kb on - strand at 403.148 kb on + strand at 403.148 kb on + strand at 403.149 kb on - strand at 403.159 kb on + strand at 403.493 kb on + strand, within Pf1N1B4_339 at 403.765 kb on + strand, within Pf1N1B4_339 at 403.895 kb on + strand, within Pf1N1B4_339 at 404.132 kb on - strand at 404.155 kb on - strand at 404.155 kb on - strand at 404.219 kb on - strand, within Pf1N1B4_340 at 404.383 kb on - strand, within Pf1N1B4_340 at 404.427 kb on - strand, within Pf1N1B4_340 at 404.427 kb on - strand, within Pf1N1B4_340 at 404.460 kb on - strand, within Pf1N1B4_340 at 404.466 kb on - strand, within Pf1N1B4_340 at 404.555 kb on + strand, within Pf1N1B4_340 at 404.556 kb on - strand, within Pf1N1B4_340 at 404.556 kb on - strand, within Pf1N1B4_340 at 404.556 kb on - strand, within Pf1N1B4_340 at 404.556 kb on - strand, within Pf1N1B4_340 at 404.556 kb on - strand, within Pf1N1B4_340 at 404.556 kb on - strand, within Pf1N1B4_340 at 404.556 kb on - strand, within Pf1N1B4_340 at 404.556 kb on - strand at 404.559 kb on + strand, within Pf1N1B4_340 at 404.560 kb on - strand, within Pf1N1B4_340 at 404.560 kb on - strand, within Pf1N1B4_340 at 404.560 kb on - strand, within Pf1N1B4_340 at 404.560 kb on - strand, within Pf1N1B4_340 at 404.560 kb on - strand, within Pf1N1B4_340 at 404.588 kb on + strand, within Pf1N1B4_340 at 404.589 kb on - strand, within Pf1N1B4_340 at 404.589 kb on - strand, within Pf1N1B4_340 at 404.589 kb on - strand, within Pf1N1B4_340 at 404.669 kb on + strand, within Pf1N1B4_340 at 404.669 kb on + strand, within Pf1N1B4_340 at 404.921 kb on + strand, within Pf1N1B4_340 at 404.921 kb on + strand, within Pf1N1B4_340 at 404.922 kb on - strand, within Pf1N1B4_340 at 404.922 kb on - strand, within Pf1N1B4_340 at 404.922 kb on - strand, within Pf1N1B4_340 at 404.922 kb on - strand, within Pf1N1B4_340 at 404.922 kb on - strand, within Pf1N1B4_340 at 404.922 kb on - strand, within Pf1N1B4_340 at 404.993 kb on + strand at 404.993 kb on + strand at 404.993 kb on + strand at 404.994 kb on - strand at 404.994 kb on - strand at 404.994 kb on - strand at 404.994 kb on - strand at 405.029 kb on - strand at 405.029 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction acetate 20 mM remove 402,198 + Pf1N1B4_338 0.41 -1.7 402,443 - Pf1N1B4_338 0.65 +1.4 402,474 - -0.2 402,554 - Pf1N1B4_338 0.76 +2.2 402,881 - +0.3 402,930 - -1.0 403,004 - -0.0 403,016 + +0.8 403,017 - -0.2 403,017 - +0.3 403,017 - +0.8 403,017 - +0.8 403,148 + -0.2 403,148 + +0.2 403,149 - -0.6 403,159 + -0.2 403,493 + Pf1N1B4_339 0.35 -1.0 403,765 + Pf1N1B4_339 0.63 -0.2 403,895 + Pf1N1B4_339 0.77 -0.7 404,132 - +1.6 404,155 - +0.3 404,155 - -2.1 404,219 - Pf1N1B4_340 0.11 +0.0 404,383 - Pf1N1B4_340 0.29 +0.4 404,427 - Pf1N1B4_340 0.34 -1.3 404,427 - Pf1N1B4_340 0.34 +1.4 404,460 - Pf1N1B4_340 0.38 -0.6 404,466 - Pf1N1B4_340 0.38 +1.4 404,555 + Pf1N1B4_340 0.48 -0.5 404,556 - Pf1N1B4_340 0.48 -0.9 404,556 - Pf1N1B4_340 0.48 -1.3 404,556 - Pf1N1B4_340 0.48 -0.2 404,556 - Pf1N1B4_340 0.48 -2.2 404,556 - Pf1N1B4_340 0.48 +0.1 404,556 - Pf1N1B4_340 0.48 +0.1 404,556 - Pf1N1B4_340 0.48 -0.2 404,556 - -0.7 404,559 + Pf1N1B4_340 0.48 -1.7 404,560 - Pf1N1B4_340 0.48 +0.1 404,560 - Pf1N1B4_340 0.48 +0.2 404,560 - Pf1N1B4_340 0.48 -0.4 404,560 - Pf1N1B4_340 0.48 +0.4 404,560 - Pf1N1B4_340 0.48 +0.2 404,588 + Pf1N1B4_340 0.51 -0.5 404,589 - Pf1N1B4_340 0.52 +0.8 404,589 - Pf1N1B4_340 0.52 +0.4 404,589 - Pf1N1B4_340 0.52 -0.5 404,669 + Pf1N1B4_340 0.60 +1.2 404,669 + Pf1N1B4_340 0.60 -0.5 404,921 + Pf1N1B4_340 0.87 +0.6 404,921 + Pf1N1B4_340 0.87 +0.3 404,922 - Pf1N1B4_340 0.88 +0.4 404,922 - Pf1N1B4_340 0.88 +0.3 404,922 - Pf1N1B4_340 0.88 +0.4 404,922 - Pf1N1B4_340 0.88 +0.4 404,922 - Pf1N1B4_340 0.88 -0.3 404,922 - Pf1N1B4_340 0.88 -1.5 404,993 + -1.2 404,993 + +1.4 404,993 + -1.0 404,994 - +1.8 404,994 - +0.6 404,994 - -0.2 404,994 - +1.8 405,029 - -0.2 405,029 - -2.6
Or see this region's nucleotide sequence