Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_3317

Experiment: acetate 20 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_3314 and Pf1N1B4_3315 are separated by 18 nucleotidesPf1N1B4_3315 and Pf1N1B4_3316 are separated by 50 nucleotidesPf1N1B4_3316 and Pf1N1B4_3317 overlap by 8 nucleotidesPf1N1B4_3317 and Pf1N1B4_3318 are separated by 159 nucleotides Pf1N1B4_3314 - Tricarboxylate porin OpdH, at 3,703,010 to 3,704,302 _3314 Pf1N1B4_3315 - hypothetical protein, at 3,704,321 to 3,704,473 _3315 Pf1N1B4_3316 - Tricarboxylate transport transcriptional regulator TctD, at 3,704,524 to 3,705,195 _3316 Pf1N1B4_3317 - Tricarboxylate transport sensor protein TctE, at 3,705,188 to 3,706,576 _3317 Pf1N1B4_3318 - Predicted signal transduction protein, at 3,706,736 to 3,707,557 _3318 Position (kb) 3705 3706 3707Strain fitness (log2 ratio) -2 -1 0 1 2at 3704.310 kb on + strandat 3704.310 kb on + strandat 3704.319 kb on + strandat 3704.515 kb on + strandat 3704.515 kb on + strandat 3704.516 kb on - strandat 3704.656 kb on + strand, within Pf1N1B4_3316at 3704.924 kb on - strand, within Pf1N1B4_3316at 3705.100 kb on + strand, within Pf1N1B4_3316at 3705.100 kb on + strand, within Pf1N1B4_3316at 3705.101 kb on - strand, within Pf1N1B4_3316at 3705.101 kb on - strand, within Pf1N1B4_3316at 3705.101 kb on - strand, within Pf1N1B4_3316at 3705.101 kb on - strand, within Pf1N1B4_3316at 3705.101 kb on - strand, within Pf1N1B4_3316at 3705.101 kb on - strand, within Pf1N1B4_3316at 3705.101 kb on - strand, within Pf1N1B4_3316at 3705.101 kb on - strand, within Pf1N1B4_3316at 3705.170 kb on - strandat 3705.194 kb on - strandat 3705.311 kb on + strandat 3705.395 kb on + strand, within Pf1N1B4_3317at 3705.409 kb on + strand, within Pf1N1B4_3317at 3705.551 kb on + strand, within Pf1N1B4_3317at 3705.551 kb on + strand, within Pf1N1B4_3317at 3705.551 kb on + strand, within Pf1N1B4_3317at 3705.551 kb on + strand, within Pf1N1B4_3317at 3705.551 kb on + strand, within Pf1N1B4_3317at 3705.552 kb on - strand, within Pf1N1B4_3317at 3705.552 kb on - strand, within Pf1N1B4_3317at 3705.552 kb on - strand, within Pf1N1B4_3317at 3705.552 kb on - strand, within Pf1N1B4_3317at 3705.563 kb on + strand, within Pf1N1B4_3317at 3705.563 kb on + strand, within Pf1N1B4_3317at 3705.564 kb on - strand, within Pf1N1B4_3317at 3705.564 kb on - strand, within Pf1N1B4_3317at 3705.564 kb on - strandat 3705.958 kb on + strand, within Pf1N1B4_3317at 3705.959 kb on - strand, within Pf1N1B4_3317at 3705.959 kb on - strand, within Pf1N1B4_3317at 3706.288 kb on + strand, within Pf1N1B4_3317at 3706.638 kb on - strandat 3706.638 kb on - strandat 3706.741 kb on - strandat 3706.872 kb on + strandat 3706.873 kb on - strand, within Pf1N1B4_3318at 3706.902 kb on + strand, within Pf1N1B4_3318at 3706.902 kb on + strand, within Pf1N1B4_3318at 3706.902 kb on + strand, within Pf1N1B4_3318at 3706.902 kb on + strand, within Pf1N1B4_3318at 3706.903 kb on - strandat 3706.903 kb on - strand, within Pf1N1B4_3318at 3706.903 kb on - strand, within Pf1N1B4_3318at 3706.938 kb on + strand, within Pf1N1B4_3318at 3706.993 kb on + strand, within Pf1N1B4_3318at 3706.994 kb on - strand, within Pf1N1B4_3318at 3707.062 kb on - strand, within Pf1N1B4_3318at 3707.076 kb on + strand, within Pf1N1B4_3318at 3707.076 kb on + strand, within Pf1N1B4_3318at 3707.077 kb on - strand, within Pf1N1B4_3318at 3707.077 kb on - strand, within Pf1N1B4_3318at 3707.077 kb on - strand, within Pf1N1B4_3318at 3707.094 kb on - strand, within Pf1N1B4_3318at 3707.094 kb on - strand, within Pf1N1B4_3318at 3707.149 kb on + strand, within Pf1N1B4_3318at 3707.149 kb on + strand, within Pf1N1B4_3318at 3707.151 kb on + strand, within Pf1N1B4_3318at 3707.151 kb on + strand, within Pf1N1B4_3318at 3707.152 kb on - strand, within Pf1N1B4_3318at 3707.152 kb on - strand, within Pf1N1B4_3318at 3707.152 kb on - strand, within Pf1N1B4_3318at 3707.169 kb on + strand, within Pf1N1B4_3318at 3707.289 kb on + strand, within Pf1N1B4_3318at 3707.289 kb on + strand, within Pf1N1B4_3318at 3707.290 kb on - strand, within Pf1N1B4_3318at 3707.290 kb on - strand, within Pf1N1B4_3318

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Per-strain Table

Position Strand Gene LocusTag Fraction acetate 20 mM
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3,704,310 + +1.0
3,704,310 + +0.4
3,704,319 + -0.9
3,704,515 + -0.5
3,704,515 + +0.4
3,704,516 - +1.0
3,704,656 + Pf1N1B4_3316 0.20 +1.0
3,704,924 - Pf1N1B4_3316 0.60 +0.7
3,705,100 + Pf1N1B4_3316 0.86 +2.4
3,705,100 + Pf1N1B4_3316 0.86 -0.5
3,705,101 - Pf1N1B4_3316 0.86 +1.2
3,705,101 - Pf1N1B4_3316 0.86 +1.0
3,705,101 - Pf1N1B4_3316 0.86 -0.4
3,705,101 - Pf1N1B4_3316 0.86 +0.6
3,705,101 - Pf1N1B4_3316 0.86 -0.4
3,705,101 - Pf1N1B4_3316 0.86 -0.0
3,705,101 - Pf1N1B4_3316 0.86 -0.0
3,705,101 - Pf1N1B4_3316 0.86 -1.5
3,705,170 - -0.9
3,705,194 - -0.9
3,705,311 + -0.9
3,705,395 + Pf1N1B4_3317 0.15 -0.0
3,705,409 + Pf1N1B4_3317 0.16 -0.9
3,705,551 + Pf1N1B4_3317 0.26 -1.3
3,705,551 + Pf1N1B4_3317 0.26 -1.0
3,705,551 + Pf1N1B4_3317 0.26 +0.5
3,705,551 + Pf1N1B4_3317 0.26 +1.0
3,705,551 + Pf1N1B4_3317 0.26 -1.0
3,705,552 - Pf1N1B4_3317 0.26 -0.6
3,705,552 - Pf1N1B4_3317 0.26 -0.8
3,705,552 - Pf1N1B4_3317 0.26 +0.4
3,705,552 - Pf1N1B4_3317 0.26 -1.2
3,705,563 + Pf1N1B4_3317 0.27 -0.2
3,705,563 + Pf1N1B4_3317 0.27 +0.6
3,705,564 - Pf1N1B4_3317 0.27 -1.4
3,705,564 - Pf1N1B4_3317 0.27 -0.5
3,705,564 - -0.6
3,705,958 + Pf1N1B4_3317 0.55 -0.0
3,705,959 - Pf1N1B4_3317 0.56 -1.7
3,705,959 - Pf1N1B4_3317 0.56 -1.0
3,706,288 + Pf1N1B4_3317 0.79 +1.0
3,706,638 - +0.2
3,706,638 - +0.8
3,706,741 - -0.4
3,706,872 + -1.6
3,706,873 - Pf1N1B4_3318 0.17 +0.2
3,706,902 + Pf1N1B4_3318 0.20 +0.2
3,706,902 + Pf1N1B4_3318 0.20 -0.6
3,706,902 + Pf1N1B4_3318 0.20 +1.2
3,706,902 + Pf1N1B4_3318 0.20 +0.4
3,706,903 - +1.5
3,706,903 - Pf1N1B4_3318 0.20 +0.3
3,706,903 - Pf1N1B4_3318 0.20 -0.2
3,706,938 + Pf1N1B4_3318 0.25 -0.0
3,706,993 + Pf1N1B4_3318 0.31 -2.0
3,706,994 - Pf1N1B4_3318 0.31 +0.2
3,707,062 - Pf1N1B4_3318 0.40 -1.0
3,707,076 + Pf1N1B4_3318 0.41 -1.0
3,707,076 + Pf1N1B4_3318 0.41 +0.2
3,707,077 - Pf1N1B4_3318 0.41 +1.0
3,707,077 - Pf1N1B4_3318 0.41 -2.4
3,707,077 - Pf1N1B4_3318 0.41 +1.7
3,707,094 - Pf1N1B4_3318 0.44 -0.9
3,707,094 - Pf1N1B4_3318 0.44 -0.6
3,707,149 + Pf1N1B4_3318 0.50 -1.9
3,707,149 + Pf1N1B4_3318 0.50 +0.4
3,707,151 + Pf1N1B4_3318 0.50 -0.0
3,707,151 + Pf1N1B4_3318 0.50 -1.0
3,707,152 - Pf1N1B4_3318 0.51 -0.3
3,707,152 - Pf1N1B4_3318 0.51 +2.3
3,707,152 - Pf1N1B4_3318 0.51 -1.0
3,707,169 + Pf1N1B4_3318 0.53 +1.0
3,707,289 + Pf1N1B4_3318 0.67 +1.5
3,707,289 + Pf1N1B4_3318 0.67 -0.0
3,707,290 - Pf1N1B4_3318 0.67 +0.5
3,707,290 - Pf1N1B4_3318 0.67 +1.5

Or see this region's nucleotide sequence