Experiment: acetate 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3314 and Pf1N1B4_3315 are separated by 18 nucleotides Pf1N1B4_3315 and Pf1N1B4_3316 are separated by 50 nucleotides Pf1N1B4_3316 and Pf1N1B4_3317 overlap by 8 nucleotides Pf1N1B4_3317 and Pf1N1B4_3318 are separated by 159 nucleotides
Pf1N1B4_3314 - Tricarboxylate porin OpdH, at 3,703,010 to 3,704,302
_3314
Pf1N1B4_3315 - hypothetical protein, at 3,704,321 to 3,704,473
_3315
Pf1N1B4_3316 - Tricarboxylate transport transcriptional regulator TctD, at 3,704,524 to 3,705,195
_3316
Pf1N1B4_3317 - Tricarboxylate transport sensor protein TctE, at 3,705,188 to 3,706,576
_3317
Pf1N1B4_3318 - Predicted signal transduction protein, at 3,706,736 to 3,707,557
_3318
Position (kb)
3705
3706
3707 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3704.310 kb on + strand at 3704.310 kb on + strand at 3704.319 kb on + strand at 3704.515 kb on + strand at 3704.515 kb on + strand at 3704.516 kb on - strand at 3704.656 kb on + strand, within Pf1N1B4_3316 at 3704.924 kb on - strand, within Pf1N1B4_3316 at 3705.100 kb on + strand, within Pf1N1B4_3316 at 3705.100 kb on + strand, within Pf1N1B4_3316 at 3705.101 kb on - strand, within Pf1N1B4_3316 at 3705.101 kb on - strand, within Pf1N1B4_3316 at 3705.101 kb on - strand, within Pf1N1B4_3316 at 3705.101 kb on - strand, within Pf1N1B4_3316 at 3705.101 kb on - strand, within Pf1N1B4_3316 at 3705.101 kb on - strand, within Pf1N1B4_3316 at 3705.101 kb on - strand, within Pf1N1B4_3316 at 3705.101 kb on - strand, within Pf1N1B4_3316 at 3705.170 kb on - strand at 3705.194 kb on - strand at 3705.311 kb on + strand at 3705.395 kb on + strand, within Pf1N1B4_3317 at 3705.409 kb on + strand, within Pf1N1B4_3317 at 3705.551 kb on + strand, within Pf1N1B4_3317 at 3705.551 kb on + strand, within Pf1N1B4_3317 at 3705.551 kb on + strand, within Pf1N1B4_3317 at 3705.551 kb on + strand, within Pf1N1B4_3317 at 3705.551 kb on + strand, within Pf1N1B4_3317 at 3705.552 kb on - strand, within Pf1N1B4_3317 at 3705.552 kb on - strand, within Pf1N1B4_3317 at 3705.552 kb on - strand, within Pf1N1B4_3317 at 3705.552 kb on - strand, within Pf1N1B4_3317 at 3705.563 kb on + strand, within Pf1N1B4_3317 at 3705.563 kb on + strand, within Pf1N1B4_3317 at 3705.564 kb on - strand, within Pf1N1B4_3317 at 3705.564 kb on - strand, within Pf1N1B4_3317 at 3705.564 kb on - strand at 3705.958 kb on + strand, within Pf1N1B4_3317 at 3705.959 kb on - strand, within Pf1N1B4_3317 at 3705.959 kb on - strand, within Pf1N1B4_3317 at 3706.288 kb on + strand, within Pf1N1B4_3317 at 3706.638 kb on - strand at 3706.638 kb on - strand at 3706.741 kb on - strand at 3706.872 kb on + strand at 3706.873 kb on - strand, within Pf1N1B4_3318 at 3706.902 kb on + strand, within Pf1N1B4_3318 at 3706.902 kb on + strand, within Pf1N1B4_3318 at 3706.902 kb on + strand, within Pf1N1B4_3318 at 3706.902 kb on + strand, within Pf1N1B4_3318 at 3706.903 kb on - strand at 3706.903 kb on - strand, within Pf1N1B4_3318 at 3706.903 kb on - strand, within Pf1N1B4_3318 at 3706.938 kb on + strand, within Pf1N1B4_3318 at 3706.993 kb on + strand, within Pf1N1B4_3318 at 3706.994 kb on - strand, within Pf1N1B4_3318 at 3707.062 kb on - strand, within Pf1N1B4_3318 at 3707.076 kb on + strand, within Pf1N1B4_3318 at 3707.076 kb on + strand, within Pf1N1B4_3318 at 3707.077 kb on - strand, within Pf1N1B4_3318 at 3707.077 kb on - strand, within Pf1N1B4_3318 at 3707.077 kb on - strand, within Pf1N1B4_3318 at 3707.094 kb on - strand, within Pf1N1B4_3318 at 3707.094 kb on - strand, within Pf1N1B4_3318 at 3707.149 kb on + strand, within Pf1N1B4_3318 at 3707.149 kb on + strand, within Pf1N1B4_3318 at 3707.151 kb on + strand, within Pf1N1B4_3318 at 3707.151 kb on + strand, within Pf1N1B4_3318 at 3707.152 kb on - strand, within Pf1N1B4_3318 at 3707.152 kb on - strand, within Pf1N1B4_3318 at 3707.152 kb on - strand, within Pf1N1B4_3318 at 3707.169 kb on + strand, within Pf1N1B4_3318 at 3707.289 kb on + strand, within Pf1N1B4_3318 at 3707.289 kb on + strand, within Pf1N1B4_3318 at 3707.290 kb on - strand, within Pf1N1B4_3318 at 3707.290 kb on - strand, within Pf1N1B4_3318
Per-strain Table
Position Strand Gene LocusTag Fraction acetate 20 mM remove 3,704,310 + +1.0 3,704,310 + +0.4 3,704,319 + -0.9 3,704,515 + -0.5 3,704,515 + +0.4 3,704,516 - +1.0 3,704,656 + Pf1N1B4_3316 0.20 +1.0 3,704,924 - Pf1N1B4_3316 0.60 +0.7 3,705,100 + Pf1N1B4_3316 0.86 +2.4 3,705,100 + Pf1N1B4_3316 0.86 -0.5 3,705,101 - Pf1N1B4_3316 0.86 +1.2 3,705,101 - Pf1N1B4_3316 0.86 +1.0 3,705,101 - Pf1N1B4_3316 0.86 -0.4 3,705,101 - Pf1N1B4_3316 0.86 +0.6 3,705,101 - Pf1N1B4_3316 0.86 -0.4 3,705,101 - Pf1N1B4_3316 0.86 -0.0 3,705,101 - Pf1N1B4_3316 0.86 -0.0 3,705,101 - Pf1N1B4_3316 0.86 -1.5 3,705,170 - -0.9 3,705,194 - -0.9 3,705,311 + -0.9 3,705,395 + Pf1N1B4_3317 0.15 -0.0 3,705,409 + Pf1N1B4_3317 0.16 -0.9 3,705,551 + Pf1N1B4_3317 0.26 -1.3 3,705,551 + Pf1N1B4_3317 0.26 -1.0 3,705,551 + Pf1N1B4_3317 0.26 +0.5 3,705,551 + Pf1N1B4_3317 0.26 +1.0 3,705,551 + Pf1N1B4_3317 0.26 -1.0 3,705,552 - Pf1N1B4_3317 0.26 -0.6 3,705,552 - Pf1N1B4_3317 0.26 -0.8 3,705,552 - Pf1N1B4_3317 0.26 +0.4 3,705,552 - Pf1N1B4_3317 0.26 -1.2 3,705,563 + Pf1N1B4_3317 0.27 -0.2 3,705,563 + Pf1N1B4_3317 0.27 +0.6 3,705,564 - Pf1N1B4_3317 0.27 -1.4 3,705,564 - Pf1N1B4_3317 0.27 -0.5 3,705,564 - -0.6 3,705,958 + Pf1N1B4_3317 0.55 -0.0 3,705,959 - Pf1N1B4_3317 0.56 -1.7 3,705,959 - Pf1N1B4_3317 0.56 -1.0 3,706,288 + Pf1N1B4_3317 0.79 +1.0 3,706,638 - +0.2 3,706,638 - +0.8 3,706,741 - -0.4 3,706,872 + -1.6 3,706,873 - Pf1N1B4_3318 0.17 +0.2 3,706,902 + Pf1N1B4_3318 0.20 +0.2 3,706,902 + Pf1N1B4_3318 0.20 -0.6 3,706,902 + Pf1N1B4_3318 0.20 +1.2 3,706,902 + Pf1N1B4_3318 0.20 +0.4 3,706,903 - +1.5 3,706,903 - Pf1N1B4_3318 0.20 +0.3 3,706,903 - Pf1N1B4_3318 0.20 -0.2 3,706,938 + Pf1N1B4_3318 0.25 -0.0 3,706,993 + Pf1N1B4_3318 0.31 -2.0 3,706,994 - Pf1N1B4_3318 0.31 +0.2 3,707,062 - Pf1N1B4_3318 0.40 -1.0 3,707,076 + Pf1N1B4_3318 0.41 -1.0 3,707,076 + Pf1N1B4_3318 0.41 +0.2 3,707,077 - Pf1N1B4_3318 0.41 +1.0 3,707,077 - Pf1N1B4_3318 0.41 -2.4 3,707,077 - Pf1N1B4_3318 0.41 +1.7 3,707,094 - Pf1N1B4_3318 0.44 -0.9 3,707,094 - Pf1N1B4_3318 0.44 -0.6 3,707,149 + Pf1N1B4_3318 0.50 -1.9 3,707,149 + Pf1N1B4_3318 0.50 +0.4 3,707,151 + Pf1N1B4_3318 0.50 -0.0 3,707,151 + Pf1N1B4_3318 0.50 -1.0 3,707,152 - Pf1N1B4_3318 0.51 -0.3 3,707,152 - Pf1N1B4_3318 0.51 +2.3 3,707,152 - Pf1N1B4_3318 0.51 -1.0 3,707,169 + Pf1N1B4_3318 0.53 +1.0 3,707,289 + Pf1N1B4_3318 0.67 +1.5 3,707,289 + Pf1N1B4_3318 0.67 -0.0 3,707,290 - Pf1N1B4_3318 0.67 +0.5 3,707,290 - Pf1N1B4_3318 0.67 +1.5
Or see this region's nucleotide sequence