Experiment: acetate 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_323 and Pf1N1B4_324 are separated by 567 nucleotides Pf1N1B4_324 and Pf1N1B4_325 are separated by 535 nucleotides
Pf1N1B4_323 - Undecaprenyl-phosphate N-acetylglucosaminyl 1-phosphate transferase (EC 2.7.8.-), at 381,190 to 382,218
_323
Pf1N1B4_324 - Glycosyl transferase, group 2 family protein, at 382,786 to 383,091
_324
Pf1N1B4_325 - D amino acid oxidase (DAO) family (EC 1.4.3.3), at 383,627 to 384,769
_325
Position (kb) 382
383
384 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 382.241 kb on - strand at 382.241 kb on - strand at 382.241 kb on - strand at 382.241 kb on - strand at 382.241 kb on - strand at 382.241 kb on - strand at 382.241 kb on - strand at 382.241 kb on - strand at 382.241 kb on - strand at 382.241 kb on - strand at 382.241 kb on - strand at 382.250 kb on - strand at 382.250 kb on - strand at 382.250 kb on - strand at 382.250 kb on - strand at 382.250 kb on - strand at 382.373 kb on - strand at 382.391 kb on - strand at 382.413 kb on + strand at 382.413 kb on + strand at 382.414 kb on - strand at 382.414 kb on - strand at 382.418 kb on - strand at 382.418 kb on - strand at 382.426 kb on + strand at 382.583 kb on - strand at 382.583 kb on - strand at 382.590 kb on - strand at 382.646 kb on + strand at 382.646 kb on + strand at 382.647 kb on - strand at 382.647 kb on - strand at 382.647 kb on - strand at 382.647 kb on - strand at 382.653 kb on + strand at 382.654 kb on - strand at 382.707 kb on - strand at 382.740 kb on + strand at 382.741 kb on - strand at 382.741 kb on - strand at 382.741 kb on - strand at 382.877 kb on + strand, within Pf1N1B4_324 at 382.878 kb on - strand, within Pf1N1B4_324 at 383.009 kb on + strand, within Pf1N1B4_324 at 383.016 kb on + strand, within Pf1N1B4_324 at 383.174 kb on + strand at 383.175 kb on - strand at 383.175 kb on - strand at 383.175 kb on - strand at 383.178 kb on + strand at 383.179 kb on - strand at 383.322 kb on + strand at 383.323 kb on - strand at 383.448 kb on + strand at 383.448 kb on + strand at 383.449 kb on - strand at 383.449 kb on - strand at 383.491 kb on - strand at 383.546 kb on + strand at 383.624 kb on - strand at 383.624 kb on - strand at 383.627 kb on - strand at 383.627 kb on - strand at 383.627 kb on - strand at 383.627 kb on - strand at 383.660 kb on + strand at 383.756 kb on + strand, within Pf1N1B4_325 at 383.776 kb on - strand, within Pf1N1B4_325 at 383.881 kb on + strand, within Pf1N1B4_325 at 383.882 kb on - strand, within Pf1N1B4_325 at 383.884 kb on + strand, within Pf1N1B4_325 at 383.891 kb on + strand, within Pf1N1B4_325 at 384.042 kb on - strand, within Pf1N1B4_325 at 384.053 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction acetate 20 mM remove 382,241 - -0.5 382,241 - +0.3 382,241 - +0.4 382,241 - +0.8 382,241 - -1.2 382,241 - +0.7 382,241 - -0.3 382,241 - +1.2 382,241 - +1.0 382,241 - +1.2 382,241 - +0.8 382,250 - +0.8 382,250 - -0.2 382,250 - +0.8 382,250 - -0.5 382,250 - -0.5 382,373 - +1.6 382,391 - -0.2 382,413 + -1.2 382,413 + +0.4 382,414 - -0.2 382,414 - +2.1 382,418 - +0.6 382,418 - +0.5 382,426 + +1.6 382,583 - +0.8 382,583 - +0.6 382,590 - +0.4 382,646 + +1.6 382,646 + -0.2 382,647 - -0.4 382,647 - -0.2 382,647 - -0.5 382,647 - +1.8 382,653 + -0.4 382,654 - +0.6 382,707 - -0.5 382,740 + +0.3 382,741 - +0.3 382,741 - -0.2 382,741 - +0.1 382,877 + Pf1N1B4_324 0.30 -0.3 382,878 - Pf1N1B4_324 0.30 -2.3 383,009 + Pf1N1B4_324 0.73 -0.2 383,016 + Pf1N1B4_324 0.75 -0.9 383,174 + -0.5 383,175 - -1.7 383,175 - -0.2 383,175 - +0.1 383,178 + +0.0 383,179 - -2.2 383,322 + -1.0 383,323 - -2.9 383,448 + -0.5 383,448 + +0.1 383,449 - +0.3 383,449 - +0.3 383,491 - -0.2 383,546 + +1.2 383,624 - +0.3 383,624 - +0.3 383,627 - -1.5 383,627 - -0.5 383,627 - +0.6 383,627 - -2.0 383,660 + -1.2 383,756 + Pf1N1B4_325 0.11 -0.3 383,776 - Pf1N1B4_325 0.13 +0.8 383,881 + Pf1N1B4_325 0.22 +0.0 383,882 - Pf1N1B4_325 0.22 +0.2 383,884 + Pf1N1B4_325 0.22 -2.2 383,891 + Pf1N1B4_325 0.23 +0.8 384,042 - Pf1N1B4_325 0.36 -1.2 384,053 - -0.2
Or see this region's nucleotide sequence