Experiment: acetate 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3067 and Pf1N1B4_3068 are separated by 165 nucleotides Pf1N1B4_3068 and Pf1N1B4_3069 are separated by 116 nucleotides Pf1N1B4_3069 and Pf1N1B4_3070 are separated by 84 nucleotides Pf1N1B4_3070 and Pf1N1B4_3071 overlap by 1 nucleotides
Pf1N1B4_3067 - Glycerol-3-phosphate acyltransferase (EC 2.3.1.15), at 3,448,727 to 3,451,210
_3067
Pf1N1B4_3068 - Cold shock protein CspA, at 3,451,376 to 3,451,585
_3068
Pf1N1B4_3069 - FIG00954614: hypothetical protein, at 3,451,702 to 3,452,079
_3069
Pf1N1B4_3070 - Ribosomal RNA large subunit methyltransferase A (EC 2.1.1.51), at 3,452,164 to 3,452,973
_3070
Pf1N1B4_3071 - N-succinyl-L,L-diaminopimelate desuccinylase (EC 3.5.1.18), at 3,452,973 to 3,454,124
_3071
Position (kb)
3451
3452
3453 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3450.954 kb on + strand, within Pf1N1B4_3067 at 3451.080 kb on + strand at 3451.080 kb on + strand at 3451.080 kb on + strand at 3451.080 kb on + strand at 3451.080 kb on + strand at 3451.080 kb on + strand at 3451.080 kb on + strand at 3451.080 kb on + strand at 3451.081 kb on - strand at 3451.081 kb on - strand at 3451.081 kb on - strand at 3451.260 kb on + strand at 3451.261 kb on - strand at 3451.299 kb on + strand at 3451.303 kb on + strand at 3451.303 kb on + strand at 3451.303 kb on + strand at 3451.304 kb on - strand at 3451.304 kb on - strand at 3451.335 kb on + strand at 3451.336 kb on - strand at 3451.337 kb on + strand at 3451.337 kb on + strand at 3451.337 kb on + strand at 3451.337 kb on + strand at 3451.338 kb on - strand at 3451.354 kb on + strand at 3451.367 kb on + strand at 3451.368 kb on - strand at 3451.371 kb on + strand at 3451.371 kb on + strand at 3451.371 kb on + strand at 3451.371 kb on + strand at 3451.371 kb on + strand at 3451.371 kb on + strand at 3451.371 kb on + strand at 3451.372 kb on - strand at 3451.372 kb on - strand at 3451.372 kb on - strand at 3451.372 kb on - strand at 3451.372 kb on - strand at 3451.372 kb on - strand at 3451.372 kb on - strand at 3451.372 kb on - strand at 3451.372 kb on - strand at 3451.376 kb on - strand at 3451.427 kb on + strand, within Pf1N1B4_3068 at 3451.472 kb on + strand, within Pf1N1B4_3068 at 3451.529 kb on + strand, within Pf1N1B4_3068 at 3451.529 kb on + strand, within Pf1N1B4_3068 at 3451.575 kb on + strand at 3451.575 kb on + strand at 3451.575 kb on + strand at 3451.575 kb on + strand at 3451.576 kb on - strand at 3451.576 kb on - strand at 3451.576 kb on - strand at 3451.576 kb on - strand at 3451.576 kb on - strand at 3451.576 kb on - strand at 3451.958 kb on + strand, within Pf1N1B4_3069 at 3451.958 kb on + strand, within Pf1N1B4_3069 at 3451.959 kb on - strand, within Pf1N1B4_3069 at 3451.959 kb on - strand, within Pf1N1B4_3069 at 3452.030 kb on + strand, within Pf1N1B4_3069 at 3452.030 kb on + strand, within Pf1N1B4_3069 at 3452.030 kb on + strand, within Pf1N1B4_3069 at 3452.031 kb on - strand, within Pf1N1B4_3069 at 3452.054 kb on + strand at 3452.181 kb on - strand at 3452.411 kb on + strand, within Pf1N1B4_3070 at 3452.412 kb on - strand, within Pf1N1B4_3070 at 3452.412 kb on - strand, within Pf1N1B4_3070 at 3452.481 kb on + strand, within Pf1N1B4_3070 at 3452.482 kb on - strand, within Pf1N1B4_3070 at 3452.690 kb on + strand, within Pf1N1B4_3070 at 3452.690 kb on + strand, within Pf1N1B4_3070 at 3452.690 kb on + strand, within Pf1N1B4_3070 at 3452.690 kb on + strand, within Pf1N1B4_3070 at 3452.690 kb on + strand, within Pf1N1B4_3070 at 3452.691 kb on - strand, within Pf1N1B4_3070 at 3452.691 kb on - strand, within Pf1N1B4_3070 at 3452.693 kb on + strand, within Pf1N1B4_3070 at 3452.693 kb on + strand, within Pf1N1B4_3070 at 3452.694 kb on - strand, within Pf1N1B4_3070 at 3452.694 kb on - strand, within Pf1N1B4_3070 at 3452.736 kb on - strand, within Pf1N1B4_3070 at 3452.778 kb on - strand, within Pf1N1B4_3070 at 3452.850 kb on + strand, within Pf1N1B4_3070 at 3452.850 kb on + strand, within Pf1N1B4_3070 at 3452.850 kb on + strand at 3452.851 kb on - strand, within Pf1N1B4_3070 at 3452.851 kb on - strand, within Pf1N1B4_3070 at 3452.851 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction acetate 20 mM remove 3,450,954 + Pf1N1B4_3067 0.90 +0.9 3,451,080 + +0.5 3,451,080 + -0.3 3,451,080 + -0.3 3,451,080 + -0.3 3,451,080 + +1.5 3,451,080 + +0.1 3,451,080 + +0.0 3,451,080 + +1.0 3,451,081 - +0.4 3,451,081 - -1.0 3,451,081 - -1.4 3,451,260 + -0.2 3,451,261 - +1.9 3,451,299 + +0.7 3,451,303 + -0.0 3,451,303 + +0.5 3,451,303 + -0.1 3,451,304 - +0.2 3,451,304 - +0.8 3,451,335 + -0.4 3,451,336 - +0.5 3,451,337 + +2.3 3,451,337 + +0.5 3,451,337 + +0.5 3,451,337 + -1.6 3,451,338 - +0.5 3,451,354 + -1.5 3,451,367 + +0.3 3,451,368 - +1.0 3,451,371 + -0.2 3,451,371 + +0.7 3,451,371 + -0.2 3,451,371 + -1.5 3,451,371 + -0.3 3,451,371 + +1.5 3,451,371 + +0.9 3,451,372 - +1.0 3,451,372 - +0.7 3,451,372 - -0.3 3,451,372 - -2.1 3,451,372 - +0.5 3,451,372 - +0.5 3,451,372 - -0.5 3,451,372 - -0.3 3,451,372 - -0.3 3,451,376 - +1.8 3,451,427 + Pf1N1B4_3068 0.24 +1.0 3,451,472 + Pf1N1B4_3068 0.46 -0.5 3,451,529 + Pf1N1B4_3068 0.73 -1.0 3,451,529 + Pf1N1B4_3068 0.73 +0.7 3,451,575 + -0.8 3,451,575 + -0.3 3,451,575 + +0.5 3,451,575 + +0.6 3,451,576 - -0.5 3,451,576 - +1.0 3,451,576 - +0.6 3,451,576 - -1.0 3,451,576 - +0.3 3,451,576 - +1.0 3,451,958 + Pf1N1B4_3069 0.68 +0.0 3,451,958 + Pf1N1B4_3069 0.68 -0.5 3,451,959 - Pf1N1B4_3069 0.68 +3.0 3,451,959 - Pf1N1B4_3069 0.68 -0.5 3,452,030 + Pf1N1B4_3069 0.87 -2.0 3,452,030 + Pf1N1B4_3069 0.87 -1.4 3,452,030 + Pf1N1B4_3069 0.87 -2.0 3,452,031 - Pf1N1B4_3069 0.87 -0.1 3,452,054 + +0.1 3,452,181 - -0.8 3,452,411 + Pf1N1B4_3070 0.30 -1.5 3,452,412 - Pf1N1B4_3070 0.31 +0.4 3,452,412 - Pf1N1B4_3070 0.31 +0.0 3,452,481 + Pf1N1B4_3070 0.39 +0.6 3,452,482 - Pf1N1B4_3070 0.39 +0.0 3,452,690 + Pf1N1B4_3070 0.65 +0.6 3,452,690 + Pf1N1B4_3070 0.65 +1.6 3,452,690 + Pf1N1B4_3070 0.65 +0.9 3,452,690 + Pf1N1B4_3070 0.65 -0.4 3,452,690 + Pf1N1B4_3070 0.65 -0.7 3,452,691 - Pf1N1B4_3070 0.65 -0.7 3,452,691 - Pf1N1B4_3070 0.65 +0.0 3,452,693 + Pf1N1B4_3070 0.65 +0.3 3,452,693 + Pf1N1B4_3070 0.65 -2.3 3,452,694 - Pf1N1B4_3070 0.65 +0.0 3,452,694 - Pf1N1B4_3070 0.65 +1.4 3,452,736 - Pf1N1B4_3070 0.71 -1.3 3,452,778 - Pf1N1B4_3070 0.76 +0.9 3,452,850 + Pf1N1B4_3070 0.85 -0.5 3,452,850 + Pf1N1B4_3070 0.85 +0.1 3,452,850 + +0.0 3,452,851 - Pf1N1B4_3070 0.85 -0.7 3,452,851 - Pf1N1B4_3070 0.85 -0.7 3,452,851 - +1.0
Or see this region's nucleotide sequence