Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_275

Experiment: acetate 20 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_274 and Pf1N1B4_275 are separated by 69 nucleotidesPf1N1B4_275 and Pf1N1B4_276 are separated by 350 nucleotidesPf1N1B4_276 and Pf1N1B4_277 are separated by 32 nucleotides Pf1N1B4_274 - Transcriptional regulator, LysR family, at 333,631 to 334,623 _274 Pf1N1B4_275 - Organic hydroperoxide resistance transcriptional regulator, at 334,693 to 335,154 _275 Pf1N1B4_276 - Organic hydroperoxide resistance protein, at 335,505 to 335,924 _276 Pf1N1B4_277 - Lipase, at 335,957 to 336,973 _277 Position (kb) 334 335 336Strain fitness (log2 ratio) -2 -1 0 1 2 3at 333.933 kb on + strandat 333.934 kb on - strand, within Pf1N1B4_274at 334.109 kb on + strand, within Pf1N1B4_274at 334.109 kb on + strand, within Pf1N1B4_274at 334.109 kb on + strand, within Pf1N1B4_274at 334.110 kb on - strand, within Pf1N1B4_274at 334.112 kb on + strand, within Pf1N1B4_274at 334.112 kb on + strand, within Pf1N1B4_274at 334.112 kb on + strand, within Pf1N1B4_274at 334.112 kb on + strand, within Pf1N1B4_274at 334.112 kb on + strand, within Pf1N1B4_274at 334.113 kb on - strand, within Pf1N1B4_274at 334.113 kb on - strand, within Pf1N1B4_274at 334.430 kb on + strand, within Pf1N1B4_274at 334.430 kb on + strand, within Pf1N1B4_274at 334.430 kb on + strand, within Pf1N1B4_274at 334.430 kb on + strand, within Pf1N1B4_274at 334.431 kb on - strand, within Pf1N1B4_274at 334.431 kb on - strandat 334.431 kb on - strandat 334.431 kb on - strand, within Pf1N1B4_274at 334.433 kb on + strand, within Pf1N1B4_274at 334.498 kb on + strandat 334.498 kb on + strand, within Pf1N1B4_274at 334.498 kb on + strand, within Pf1N1B4_274at 334.499 kb on - strand, within Pf1N1B4_274at 334.499 kb on - strand, within Pf1N1B4_274at 334.499 kb on - strand, within Pf1N1B4_274at 334.499 kb on - strand, within Pf1N1B4_274at 334.552 kb on + strandat 334.622 kb on - strandat 334.795 kb on + strand, within Pf1N1B4_275at 334.796 kb on - strand, within Pf1N1B4_275at 334.796 kb on - strand, within Pf1N1B4_275at 334.828 kb on + strand, within Pf1N1B4_275at 334.994 kb on + strand, within Pf1N1B4_275at 334.994 kb on + strand, within Pf1N1B4_275at 334.995 kb on - strandat 335.130 kb on + strandat 335.131 kb on - strandat 335.131 kb on - strandat 335.145 kb on + strandat 335.348 kb on + strandat 335.348 kb on + strandat 335.348 kb on + strandat 335.349 kb on - strandat 335.349 kb on - strandat 335.356 kb on + strandat 335.357 kb on - strandat 335.421 kb on - strandat 335.518 kb on - strandat 335.845 kb on + strand, within Pf1N1B4_276at 335.845 kb on + strand, within Pf1N1B4_276at 335.846 kb on - strand, within Pf1N1B4_276at 335.846 kb on - strand, within Pf1N1B4_276at 335.846 kb on - strand, within Pf1N1B4_276at 335.922 kb on + strandat 335.922 kb on + strandat 335.923 kb on - strandat 335.923 kb on - strandat 335.923 kb on - strandat 335.923 kb on - strandat 335.956 kb on + strandat 335.956 kb on + strandat 335.956 kb on + strandat 335.957 kb on - strandat 335.957 kb on - strandat 335.957 kb on - strandat 335.957 kb on - strandat 336.063 kb on + strand, within Pf1N1B4_277at 336.063 kb on + strand, within Pf1N1B4_277at 336.064 kb on - strand, within Pf1N1B4_277at 336.064 kb on - strand, within Pf1N1B4_277at 336.064 kb on - strand, within Pf1N1B4_277at 336.064 kb on - strand, within Pf1N1B4_277at 336.064 kb on - strand, within Pf1N1B4_277at 336.064 kb on - strandat 336.065 kb on - strand, within Pf1N1B4_277at 336.147 kb on + strand, within Pf1N1B4_277

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Per-strain Table

Position Strand Gene LocusTag Fraction acetate 20 mM
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333,933 + +0.1
333,934 - Pf1N1B4_274 0.31 +0.4
334,109 + Pf1N1B4_274 0.48 +0.1
334,109 + Pf1N1B4_274 0.48 -0.8
334,109 + Pf1N1B4_274 0.48 +0.2
334,110 - Pf1N1B4_274 0.48 +0.1
334,112 + Pf1N1B4_274 0.48 +0.8
334,112 + Pf1N1B4_274 0.48 +2.0
334,112 + Pf1N1B4_274 0.48 +1.2
334,112 + Pf1N1B4_274 0.48 +0.8
334,112 + Pf1N1B4_274 0.48 -1.3
334,113 - Pf1N1B4_274 0.49 -0.3
334,113 - Pf1N1B4_274 0.49 -0.1
334,430 + Pf1N1B4_274 0.80 +0.8
334,430 + Pf1N1B4_274 0.80 +0.8
334,430 + Pf1N1B4_274 0.80 +0.5
334,430 + Pf1N1B4_274 0.80 +0.0
334,431 - Pf1N1B4_274 0.81 +3.1
334,431 - +2.8
334,431 - -1.2
334,431 - Pf1N1B4_274 0.81 -2.4
334,433 + Pf1N1B4_274 0.81 -1.8
334,498 + +0.8
334,498 + Pf1N1B4_274 0.87 +2.4
334,498 + Pf1N1B4_274 0.87 -0.8
334,499 - Pf1N1B4_274 0.87 -0.8
334,499 - Pf1N1B4_274 0.87 -0.4
334,499 - Pf1N1B4_274 0.87 +1.0
334,499 - Pf1N1B4_274 0.87 -0.6
334,552 + +0.5
334,622 - +0.2
334,795 + Pf1N1B4_275 0.22 -0.5
334,796 - Pf1N1B4_275 0.22 -2.4
334,796 - Pf1N1B4_275 0.22 +0.2
334,828 + Pf1N1B4_275 0.29 -0.9
334,994 + Pf1N1B4_275 0.65 -0.2
334,994 + Pf1N1B4_275 0.65 +1.1
334,995 - +0.8
335,130 + -0.5
335,131 - -0.9
335,131 - +0.6
335,145 + -0.8
335,348 + -0.7
335,348 + +0.1
335,348 + -1.5
335,349 - +1.2
335,349 - -1.5
335,356 + -0.8
335,357 - +0.8
335,421 - -0.3
335,518 - -0.3
335,845 + Pf1N1B4_276 0.81 +0.0
335,845 + Pf1N1B4_276 0.81 +0.1
335,846 - Pf1N1B4_276 0.81 -0.9
335,846 - Pf1N1B4_276 0.81 +0.1
335,846 - Pf1N1B4_276 0.81 +0.6
335,922 + -0.6
335,922 + -1.2
335,923 - -0.8
335,923 - +0.8
335,923 - +0.7
335,923 - -0.2
335,956 + -0.8
335,956 + +0.5
335,956 + +1.0
335,957 - +1.8
335,957 - +0.3
335,957 - +0.8
335,957 - -0.5
336,063 + Pf1N1B4_277 0.10 -1.8
336,063 + Pf1N1B4_277 0.10 +0.6
336,064 - Pf1N1B4_277 0.11 +0.8
336,064 - Pf1N1B4_277 0.11 -0.2
336,064 - Pf1N1B4_277 0.11 +0.6
336,064 - Pf1N1B4_277 0.11 -0.8
336,064 - Pf1N1B4_277 0.11 +0.2
336,064 - +0.2
336,065 - Pf1N1B4_277 0.11 +1.1
336,147 + Pf1N1B4_277 0.19 -1.2

Or see this region's nucleotide sequence