Experiment: acetate 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2338 and Pf1N1B4_2339 are separated by 183 nucleotides Pf1N1B4_2339 and Pf1N1B4_2340 overlap by 1 nucleotides Pf1N1B4_2340 and Pf1N1B4_2341 are separated by 21 nucleotides
Pf1N1B4_2338 - Homoserine O-acetyltransferase (EC 2.3.1.31), at 2,654,730 to 2,655,869
_2338
Pf1N1B4_2339 - COG1872, at 2,656,053 to 2,656,349
_2339
Pf1N1B4_2340 - Integral membrane protein YggT, involved in response to extracytoplasmic stress (osmotic shock), at 2,656,349 to 2,656,939
_2340
Pf1N1B4_2341 - Pyrroline-5-carboxylate reductase (EC 1.5.1.2), at 2,656,961 to 2,657,779
_2341
Position (kb)
2656
2657 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 2655.062 kb on - strand, within Pf1N1B4_2338 at 2655.070 kb on + strand, within Pf1N1B4_2338 at 2655.070 kb on + strand, within Pf1N1B4_2338 at 2655.070 kb on + strand, within Pf1N1B4_2338 at 2655.070 kb on + strand, within Pf1N1B4_2338 at 2655.071 kb on - strand, within Pf1N1B4_2338 at 2655.071 kb on - strand, within Pf1N1B4_2338 at 2655.106 kb on + strand, within Pf1N1B4_2338 at 2655.106 kb on + strand, within Pf1N1B4_2338 at 2655.107 kb on - strand, within Pf1N1B4_2338 at 2655.107 kb on - strand, within Pf1N1B4_2338 at 2655.169 kb on + strand, within Pf1N1B4_2338 at 2655.169 kb on + strand, within Pf1N1B4_2338 at 2655.169 kb on + strand, within Pf1N1B4_2338 at 2655.169 kb on + strand, within Pf1N1B4_2338 at 2655.169 kb on + strand, within Pf1N1B4_2338 at 2655.169 kb on + strand, within Pf1N1B4_2338 at 2655.169 kb on + strand, within Pf1N1B4_2338 at 2655.169 kb on + strand, within Pf1N1B4_2338 at 2655.169 kb on + strand, within Pf1N1B4_2338 at 2655.170 kb on - strand, within Pf1N1B4_2338 at 2655.170 kb on - strand, within Pf1N1B4_2338 at 2655.170 kb on - strand, within Pf1N1B4_2338 at 2655.170 kb on - strand, within Pf1N1B4_2338 at 2655.569 kb on + strand, within Pf1N1B4_2338 at 2655.569 kb on + strand at 2655.569 kb on + strand, within Pf1N1B4_2338 at 2655.570 kb on - strand, within Pf1N1B4_2338 at 2655.745 kb on + strand, within Pf1N1B4_2338 at 2655.745 kb on + strand, within Pf1N1B4_2338 at 2655.746 kb on - strand, within Pf1N1B4_2338 at 2655.754 kb on + strand, within Pf1N1B4_2338 at 2655.754 kb on + strand, within Pf1N1B4_2338 at 2655.754 kb on + strand, within Pf1N1B4_2338 at 2655.766 kb on + strand at 2655.766 kb on + strand at 2655.766 kb on + strand at 2655.766 kb on + strand at 2655.766 kb on + strand at 2655.767 kb on - strand at 2655.767 kb on - strand at 2655.897 kb on + strand at 2655.897 kb on + strand at 2655.897 kb on + strand at 2655.897 kb on + strand at 2655.898 kb on - strand at 2655.898 kb on - strand at 2655.903 kb on + strand at 2655.903 kb on + strand at 2656.201 kb on + strand, within Pf1N1B4_2339 at 2656.202 kb on - strand, within Pf1N1B4_2339 at 2656.202 kb on - strand, within Pf1N1B4_2339 at 2656.202 kb on - strand, within Pf1N1B4_2339 at 2656.342 kb on + strand at 2656.342 kb on + strand at 2656.342 kb on + strand at 2656.343 kb on - strand at 2656.343 kb on - strand at 2656.343 kb on - strand at 2656.343 kb on - strand at 2656.386 kb on + strand at 2656.386 kb on + strand at 2656.386 kb on + strand at 2656.387 kb on - strand at 2656.387 kb on - strand at 2656.387 kb on - strand at 2656.387 kb on - strand at 2656.387 kb on - strand at 2656.387 kb on - strand at 2656.389 kb on + strand at 2656.952 kb on - strand at 2656.952 kb on - strand at 2656.952 kb on - strand at 2656.952 kb on - strand at 2656.952 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction acetate 20 mM remove 2,655,062 - Pf1N1B4_2338 0.29 -1.2 2,655,070 + Pf1N1B4_2338 0.30 +0.1 2,655,070 + Pf1N1B4_2338 0.30 +1.9 2,655,070 + Pf1N1B4_2338 0.30 +1.0 2,655,070 + Pf1N1B4_2338 0.30 -1.7 2,655,071 - Pf1N1B4_2338 0.30 +0.4 2,655,071 - Pf1N1B4_2338 0.30 +3.3 2,655,106 + Pf1N1B4_2338 0.33 +0.7 2,655,106 + Pf1N1B4_2338 0.33 -0.4 2,655,107 - Pf1N1B4_2338 0.33 -2.2 2,655,107 - Pf1N1B4_2338 0.33 -0.3 2,655,169 + Pf1N1B4_2338 0.39 -1.2 2,655,169 + Pf1N1B4_2338 0.39 +0.8 2,655,169 + Pf1N1B4_2338 0.39 +0.1 2,655,169 + Pf1N1B4_2338 0.39 +0.6 2,655,169 + Pf1N1B4_2338 0.39 +1.5 2,655,169 + Pf1N1B4_2338 0.39 +0.1 2,655,169 + Pf1N1B4_2338 0.39 +0.5 2,655,169 + Pf1N1B4_2338 0.39 +2.6 2,655,169 + Pf1N1B4_2338 0.39 +0.1 2,655,170 - Pf1N1B4_2338 0.39 -2.4 2,655,170 - Pf1N1B4_2338 0.39 +0.3 2,655,170 - Pf1N1B4_2338 0.39 +1.3 2,655,170 - Pf1N1B4_2338 0.39 +0.9 2,655,569 + Pf1N1B4_2338 0.74 +0.3 2,655,569 + +1.3 2,655,569 + Pf1N1B4_2338 0.74 +0.9 2,655,570 - Pf1N1B4_2338 0.74 -0.3 2,655,745 + Pf1N1B4_2338 0.89 +0.3 2,655,745 + Pf1N1B4_2338 0.89 -0.3 2,655,746 - Pf1N1B4_2338 0.89 +0.7 2,655,754 + Pf1N1B4_2338 0.90 +1.3 2,655,754 + Pf1N1B4_2338 0.90 +0.7 2,655,754 + Pf1N1B4_2338 0.90 -0.2 2,655,766 + -0.2 2,655,766 + -0.1 2,655,766 + -2.2 2,655,766 + -1.7 2,655,766 + +0.9 2,655,767 - -0.1 2,655,767 - +0.0 2,655,897 + +1.3 2,655,897 + +0.8 2,655,897 + -0.4 2,655,897 + +0.1 2,655,898 - -1.6 2,655,898 - -0.1 2,655,903 + -1.5 2,655,903 + +0.7 2,656,201 + Pf1N1B4_2339 0.50 -0.3 2,656,202 - Pf1N1B4_2339 0.50 -0.5 2,656,202 - Pf1N1B4_2339 0.50 -0.4 2,656,202 - Pf1N1B4_2339 0.50 -0.2 2,656,342 + +0.5 2,656,342 + -0.4 2,656,342 + -0.7 2,656,343 - +0.3 2,656,343 - +0.5 2,656,343 - -0.1 2,656,343 - +0.4 2,656,386 + +0.5 2,656,386 + +0.7 2,656,386 + -1.0 2,656,387 - +0.7 2,656,387 - +4.1 2,656,387 - -2.3 2,656,387 - +0.6 2,656,387 - +0.3 2,656,387 - -0.1 2,656,389 + +0.3 2,656,952 - -0.3 2,656,952 - -1.0 2,656,952 - +1.3 2,656,952 - +0.8 2,656,952 - +0.3
Or see this region's nucleotide sequence