Experiment: acetate 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1965 and Pf1N1B4_1966 overlap by 4 nucleotides Pf1N1B4_1966 and Pf1N1B4_1967 overlap by 21 nucleotides Pf1N1B4_1967 and Pf1N1B4_1968 are separated by 318 nucleotides
Pf1N1B4_1965 - GDP-mannose 4,6-dehydratase (EC 4.2.1.47), at 2,246,028 to 2,246,999
_1965
Pf1N1B4_1966 - UDP-glucose 4-epimerase (EC 5.1.3.2), at 2,246,996 to 2,247,754
_1966
Pf1N1B4_1967 - hypothetical protein, at 2,247,734 to 2,248,021
_1967
Pf1N1B4_1968 - Glycosyltransferase, at 2,248,340 to 2,249,917
_1968
Position (kb)
2247
2248
2249 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2246.764 kb on + strand, within Pf1N1B4_1965 at 2246.764 kb on + strand, within Pf1N1B4_1965 at 2246.765 kb on - strand, within Pf1N1B4_1965 at 2246.765 kb on - strand, within Pf1N1B4_1965 at 2246.837 kb on - strand, within Pf1N1B4_1965 at 2246.918 kb on + strand at 2246.918 kb on + strand at 2246.918 kb on + strand at 2246.918 kb on + strand at 2246.918 kb on + strand at 2246.919 kb on - strand at 2246.919 kb on - strand at 2246.919 kb on - strand at 2246.919 kb on - strand at 2246.919 kb on - strand at 2246.919 kb on - strand at 2246.919 kb on - strand at 2246.919 kb on - strand at 2246.919 kb on - strand at 2246.920 kb on + strand at 2246.950 kb on + strand at 2247.232 kb on + strand, within Pf1N1B4_1966 at 2247.233 kb on - strand, within Pf1N1B4_1966 at 2247.233 kb on - strand, within Pf1N1B4_1966 at 2247.233 kb on - strand, within Pf1N1B4_1966 at 2247.233 kb on - strand, within Pf1N1B4_1966 at 2247.237 kb on + strand, within Pf1N1B4_1966 at 2247.237 kb on + strand, within Pf1N1B4_1966 at 2247.237 kb on + strand, within Pf1N1B4_1966 at 2247.237 kb on + strand, within Pf1N1B4_1966 at 2247.237 kb on + strand, within Pf1N1B4_1966 at 2247.237 kb on + strand, within Pf1N1B4_1966 at 2247.238 kb on - strand, within Pf1N1B4_1966 at 2247.238 kb on - strand, within Pf1N1B4_1966 at 2247.276 kb on + strand, within Pf1N1B4_1966 at 2247.276 kb on + strand, within Pf1N1B4_1966 at 2247.276 kb on + strand, within Pf1N1B4_1966 at 2247.277 kb on - strand, within Pf1N1B4_1966 at 2247.277 kb on - strand, within Pf1N1B4_1966 at 2247.519 kb on + strand, within Pf1N1B4_1966 at 2247.585 kb on + strand, within Pf1N1B4_1966 at 2247.585 kb on + strand, within Pf1N1B4_1966 at 2247.586 kb on - strand, within Pf1N1B4_1966 at 2247.606 kb on + strand, within Pf1N1B4_1966 at 2247.606 kb on + strand, within Pf1N1B4_1966 at 2247.607 kb on - strand, within Pf1N1B4_1966 at 2247.607 kb on - strand, within Pf1N1B4_1966 at 2247.607 kb on - strand, within Pf1N1B4_1966 at 2247.607 kb on - strand, within Pf1N1B4_1966 at 2247.607 kb on - strand, within Pf1N1B4_1966 at 2247.616 kb on + strand, within Pf1N1B4_1966 at 2247.617 kb on - strand, within Pf1N1B4_1966 at 2247.685 kb on + strand at 2247.685 kb on + strand at 2247.686 kb on - strand at 2247.686 kb on - strand at 2247.789 kb on - strand, within Pf1N1B4_1967 at 2247.789 kb on - strand, within Pf1N1B4_1967 at 2247.789 kb on - strand, within Pf1N1B4_1967 at 2247.869 kb on + strand, within Pf1N1B4_1967 at 2247.870 kb on - strand, within Pf1N1B4_1967 at 2247.870 kb on - strand, within Pf1N1B4_1967 at 2247.931 kb on + strand, within Pf1N1B4_1967 at 2247.993 kb on + strand at 2248.019 kb on + strand at 2248.019 kb on + strand at 2248.020 kb on - strand at 2248.020 kb on - strand at 2248.105 kb on + strand at 2248.106 kb on - strand at 2248.106 kb on - strand at 2248.109 kb on + strand at 2248.109 kb on + strand at 2248.109 kb on + strand at 2248.109 kb on + strand at 2248.109 kb on + strand at 2248.109 kb on + strand at 2248.150 kb on + strand at 2248.150 kb on + strand at 2248.150 kb on + strand at 2248.150 kb on + strand at 2248.150 kb on + strand at 2248.150 kb on + strand at 2248.150 kb on + strand at 2248.151 kb on - strand at 2248.151 kb on - strand at 2248.151 kb on - strand at 2248.191 kb on + strand at 2248.191 kb on + strand at 2248.191 kb on + strand at 2248.219 kb on + strand at 2248.219 kb on + strand at 2248.220 kb on - strand at 2248.220 kb on - strand at 2248.232 kb on + strand at 2248.232 kb on + strand at 2248.232 kb on + strand at 2248.232 kb on + strand at 2248.232 kb on + strand at 2248.232 kb on + strand at 2248.232 kb on + strand at 2248.233 kb on - strand at 2248.233 kb on - strand at 2248.233 kb on - strand at 2248.240 kb on + strand at 2248.268 kb on + strand at 2248.268 kb on + strand at 2248.298 kb on + strand at 2248.298 kb on + strand at 2248.298 kb on + strand at 2248.298 kb on + strand at 2248.299 kb on - strand at 2248.299 kb on - strand at 2248.339 kb on + strand at 2248.340 kb on - strand at 2248.356 kb on - strand at 2248.356 kb on - strand at 2248.406 kb on + strand at 2248.406 kb on + strand at 2248.468 kb on + strand at 2248.468 kb on + strand at 2248.468 kb on + strand at 2248.468 kb on + strand at 2248.469 kb on - strand at 2248.469 kb on - strand at 2248.469 kb on - strand at 2248.534 kb on + strand, within Pf1N1B4_1968 at 2248.534 kb on + strand, within Pf1N1B4_1968 at 2248.534 kb on + strand, within Pf1N1B4_1968 at 2248.535 kb on - strand, within Pf1N1B4_1968 at 2248.847 kb on - strand, within Pf1N1B4_1968 at 2248.879 kb on + strand, within Pf1N1B4_1968 at 2248.879 kb on + strand, within Pf1N1B4_1968 at 2248.880 kb on - strand, within Pf1N1B4_1968 at 2248.997 kb on + strand, within Pf1N1B4_1968 at 2248.998 kb on - strand, within Pf1N1B4_1968 at 2248.998 kb on - strand, within Pf1N1B4_1968 at 2249.014 kb on + strand, within Pf1N1B4_1968
Per-strain Table
Position Strand Gene LocusTag Fraction acetate 20 mM remove 2,246,764 + Pf1N1B4_1965 0.76 -0.9 2,246,764 + Pf1N1B4_1965 0.76 +0.4 2,246,765 - Pf1N1B4_1965 0.76 -0.6 2,246,765 - Pf1N1B4_1965 0.76 +0.4 2,246,837 - Pf1N1B4_1965 0.83 -0.2 2,246,918 + -0.2 2,246,918 + +0.4 2,246,918 + +1.2 2,246,918 + -1.3 2,246,918 + +1.4 2,246,919 - +0.2 2,246,919 - -0.7 2,246,919 - +0.5 2,246,919 - -1.4 2,246,919 - +3.0 2,246,919 - +0.2 2,246,919 - -1.6 2,246,919 - +2.4 2,246,919 - +0.1 2,246,920 + +0.2 2,246,950 + +0.4 2,247,232 + Pf1N1B4_1966 0.31 -0.3 2,247,233 - Pf1N1B4_1966 0.31 +1.4 2,247,233 - Pf1N1B4_1966 0.31 +1.4 2,247,233 - Pf1N1B4_1966 0.31 +0.6 2,247,233 - Pf1N1B4_1966 0.31 +0.0 2,247,237 + Pf1N1B4_1966 0.32 -1.2 2,247,237 + Pf1N1B4_1966 0.32 -0.9 2,247,237 + Pf1N1B4_1966 0.32 +0.8 2,247,237 + Pf1N1B4_1966 0.32 +0.9 2,247,237 + Pf1N1B4_1966 0.32 -0.6 2,247,237 + Pf1N1B4_1966 0.32 -0.9 2,247,238 - Pf1N1B4_1966 0.32 -1.2 2,247,238 - Pf1N1B4_1966 0.32 +1.2 2,247,276 + Pf1N1B4_1966 0.37 +0.9 2,247,276 + Pf1N1B4_1966 0.37 -1.2 2,247,276 + Pf1N1B4_1966 0.37 -0.1 2,247,277 - Pf1N1B4_1966 0.37 +0.5 2,247,277 - Pf1N1B4_1966 0.37 +0.4 2,247,519 + Pf1N1B4_1966 0.69 +1.2 2,247,585 + Pf1N1B4_1966 0.78 +0.7 2,247,585 + Pf1N1B4_1966 0.78 +0.1 2,247,586 - Pf1N1B4_1966 0.78 +0.3 2,247,606 + Pf1N1B4_1966 0.80 -1.8 2,247,606 + Pf1N1B4_1966 0.80 +0.2 2,247,607 - Pf1N1B4_1966 0.81 -0.4 2,247,607 - Pf1N1B4_1966 0.81 -2.1 2,247,607 - Pf1N1B4_1966 0.81 -0.2 2,247,607 - Pf1N1B4_1966 0.81 -0.9 2,247,607 - Pf1N1B4_1966 0.81 +0.4 2,247,616 + Pf1N1B4_1966 0.82 -0.2 2,247,617 - Pf1N1B4_1966 0.82 -0.4 2,247,685 + -0.3 2,247,685 + -0.4 2,247,686 - -0.6 2,247,686 - +0.1 2,247,789 - Pf1N1B4_1967 0.19 -0.2 2,247,789 - Pf1N1B4_1967 0.19 -1.9 2,247,789 - Pf1N1B4_1967 0.19 -1.4 2,247,869 + Pf1N1B4_1967 0.47 -0.0 2,247,870 - Pf1N1B4_1967 0.47 +0.7 2,247,870 - Pf1N1B4_1967 0.47 -0.7 2,247,931 + Pf1N1B4_1967 0.68 -0.5 2,247,993 + +0.7 2,248,019 + -1.8 2,248,019 + +0.0 2,248,020 - +0.4 2,248,020 - +1.4 2,248,105 + -0.9 2,248,106 - +0.8 2,248,106 - +0.6 2,248,109 + -0.6 2,248,109 + -0.9 2,248,109 + -1.1 2,248,109 + -0.6 2,248,109 + -1.2 2,248,109 + +0.0 2,248,150 + -0.6 2,248,150 + -0.3 2,248,150 + -0.9 2,248,150 + -0.9 2,248,150 + -1.2 2,248,150 + +1.5 2,248,150 + -1.2 2,248,151 - +1.6 2,248,151 - +0.4 2,248,151 - +0.1 2,248,191 + -0.2 2,248,191 + -0.6 2,248,191 + -1.2 2,248,219 + +0.0 2,248,219 + +0.6 2,248,220 - +0.7 2,248,220 - -0.9 2,248,232 + +0.4 2,248,232 + +0.4 2,248,232 + +0.2 2,248,232 + -0.4 2,248,232 + -0.8 2,248,232 + +0.6 2,248,232 + -0.4 2,248,233 - +0.7 2,248,233 - +0.6 2,248,233 - +0.4 2,248,240 + +0.4 2,248,268 + -0.6 2,248,268 + -1.4 2,248,298 + +0.9 2,248,298 + +0.7 2,248,298 + -0.3 2,248,298 + +0.0 2,248,299 - -0.6 2,248,299 - +1.1 2,248,339 + +0.2 2,248,340 - -0.1 2,248,356 - -0.7 2,248,356 - -0.2 2,248,406 + +0.1 2,248,406 + -0.2 2,248,468 + +0.2 2,248,468 + -0.7 2,248,468 + -0.2 2,248,468 + -0.6 2,248,469 - +0.5 2,248,469 - -0.9 2,248,469 - +1.2 2,248,534 + Pf1N1B4_1968 0.12 +0.4 2,248,534 + Pf1N1B4_1968 0.12 +0.8 2,248,534 + Pf1N1B4_1968 0.12 -0.2 2,248,535 - Pf1N1B4_1968 0.12 +0.1 2,248,847 - Pf1N1B4_1968 0.32 -0.3 2,248,879 + Pf1N1B4_1968 0.34 +0.2 2,248,879 + Pf1N1B4_1968 0.34 +1.1 2,248,880 - Pf1N1B4_1968 0.34 +0.4 2,248,997 + Pf1N1B4_1968 0.42 +0.4 2,248,998 - Pf1N1B4_1968 0.42 +1.1 2,248,998 - Pf1N1B4_1968 0.42 +2.6 2,249,014 + Pf1N1B4_1968 0.43 +0.4
Or see this region's nucleotide sequence