Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_159

Experiment: acetate 20 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_157 and Pf1N1B4_158 are separated by 37 nucleotidesPf1N1B4_158 and Pf1N1B4_159 are separated by 26 nucleotidesPf1N1B4_159 and Pf1N1B4_160 are separated by 89 nucleotides Pf1N1B4_157 - ThiJ/PfpI family protein, at 200,059 to 200,745 _157 Pf1N1B4_158 - FIG00456158: hypothetical protein, at 200,783 to 201,112 _158 Pf1N1B4_159 - Putative threonine efflux protein, at 201,139 to 201,768 _159 Pf1N1B4_160 - Transcriptional regulator, GntR family domain / Aspartate aminotransferase (EC 2.6.1.1), at 201,858 to 203,183 _160 Position (kb) 201 202Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 200.345 kb on - strand, within Pf1N1B4_157at 200.345 kb on - strand, within Pf1N1B4_157at 200.362 kb on - strand, within Pf1N1B4_157at 200.599 kb on + strand, within Pf1N1B4_157at 200.832 kb on + strand, within Pf1N1B4_158at 200.833 kb on - strand, within Pf1N1B4_158at 200.833 kb on - strandat 200.833 kb on - strand, within Pf1N1B4_158at 200.935 kb on + strand, within Pf1N1B4_158at 200.935 kb on + strand, within Pf1N1B4_158at 200.956 kb on - strandat 200.959 kb on - strand, within Pf1N1B4_158at 200.959 kb on - strand, within Pf1N1B4_158at 200.959 kb on - strand, within Pf1N1B4_158at 201.090 kb on + strandat 201.091 kb on - strandat 201.095 kb on - strandat 201.108 kb on + strandat 201.108 kb on + strandat 201.108 kb on + strandat 201.108 kb on + strandat 201.108 kb on + strandat 201.109 kb on - strandat 201.109 kb on - strandat 201.231 kb on + strandat 201.255 kb on + strand, within Pf1N1B4_159at 201.256 kb on - strand, within Pf1N1B4_159at 201.392 kb on - strand, within Pf1N1B4_159at 201.665 kb on + strand, within Pf1N1B4_159at 201.666 kb on - strand, within Pf1N1B4_159at 201.797 kb on - strandat 201.797 kb on - strandat 201.801 kb on + strandat 201.801 kb on + strandat 201.840 kb on + strandat 202.002 kb on + strand, within Pf1N1B4_160at 202.003 kb on - strandat 202.168 kb on + strand, within Pf1N1B4_160at 202.169 kb on - strand, within Pf1N1B4_160at 202.280 kb on - strand, within Pf1N1B4_160at 202.280 kb on - strand, within Pf1N1B4_160at 202.407 kb on + strand, within Pf1N1B4_160at 202.534 kb on - strand, within Pf1N1B4_160at 202.647 kb on + strand, within Pf1N1B4_160at 202.648 kb on - strand, within Pf1N1B4_160at 202.685 kb on + strand, within Pf1N1B4_160at 202.685 kb on + strand, within Pf1N1B4_160at 202.686 kb on - strand, within Pf1N1B4_160at 202.686 kb on - strand, within Pf1N1B4_160at 202.710 kb on + strand, within Pf1N1B4_160at 202.710 kb on + strand, within Pf1N1B4_160at 202.711 kb on - strand, within Pf1N1B4_160at 202.711 kb on - strand, within Pf1N1B4_160at 202.711 kb on - strand, within Pf1N1B4_160at 202.711 kb on - strand, within Pf1N1B4_160at 202.711 kb on - strand, within Pf1N1B4_160at 202.711 kb on - strand, within Pf1N1B4_160at 202.711 kb on - strand, within Pf1N1B4_160at 202.711 kb on - strand, within Pf1N1B4_160at 202.711 kb on - strandat 202.711 kb on - strand, within Pf1N1B4_160at 202.711 kb on - strand, within Pf1N1B4_160at 202.713 kb on - strand, within Pf1N1B4_160

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Per-strain Table

Position Strand Gene LocusTag Fraction acetate 20 mM
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200,345 - Pf1N1B4_157 0.42 +0.3
200,345 - Pf1N1B4_157 0.42 +1.1
200,362 - Pf1N1B4_157 0.44 -0.6
200,599 + Pf1N1B4_157 0.79 -1.6
200,832 + Pf1N1B4_158 0.15 -0.3
200,833 - Pf1N1B4_158 0.15 +0.2
200,833 - -0.2
200,833 - Pf1N1B4_158 0.15 -0.6
200,935 + Pf1N1B4_158 0.46 +0.4
200,935 + Pf1N1B4_158 0.46 -2.6
200,956 - +0.8
200,959 - Pf1N1B4_158 0.53 +1.2
200,959 - Pf1N1B4_158 0.53 +0.3
200,959 - Pf1N1B4_158 0.53 -0.0
201,090 + +0.8
201,091 - +1.0
201,095 - +0.8
201,108 + -1.2
201,108 + -0.2
201,108 + -0.1
201,108 + -0.1
201,108 + -0.2
201,109 - +1.8
201,109 - -0.5
201,231 + -0.2
201,255 + Pf1N1B4_159 0.18 -1.0
201,256 - Pf1N1B4_159 0.19 -1.2
201,392 - Pf1N1B4_159 0.40 -2.2
201,665 + Pf1N1B4_159 0.83 +0.3
201,666 - Pf1N1B4_159 0.84 -1.2
201,797 - +1.8
201,797 - -1.8
201,801 + -0.6
201,801 + -0.5
201,840 + -0.8
202,002 + Pf1N1B4_160 0.11 -0.6
202,003 - +0.2
202,168 + Pf1N1B4_160 0.23 -0.8
202,169 - Pf1N1B4_160 0.23 +1.5
202,280 - Pf1N1B4_160 0.32 +0.0
202,280 - Pf1N1B4_160 0.32 +0.5
202,407 + Pf1N1B4_160 0.41 -0.5
202,534 - Pf1N1B4_160 0.51 +0.8
202,647 + Pf1N1B4_160 0.60 +0.8
202,648 - Pf1N1B4_160 0.60 -0.1
202,685 + Pf1N1B4_160 0.62 +0.1
202,685 + Pf1N1B4_160 0.62 +0.2
202,686 - Pf1N1B4_160 0.62 -3.1
202,686 - Pf1N1B4_160 0.62 -1.2
202,710 + Pf1N1B4_160 0.64 -1.0
202,710 + Pf1N1B4_160 0.64 -0.5
202,711 - Pf1N1B4_160 0.64 +0.2
202,711 - Pf1N1B4_160 0.64 +0.0
202,711 - Pf1N1B4_160 0.64 +0.1
202,711 - Pf1N1B4_160 0.64 +1.1
202,711 - Pf1N1B4_160 0.64 -0.2
202,711 - Pf1N1B4_160 0.64 -0.4
202,711 - Pf1N1B4_160 0.64 +2.0
202,711 - Pf1N1B4_160 0.64 -1.6
202,711 - +1.2
202,711 - Pf1N1B4_160 0.64 +0.8
202,711 - Pf1N1B4_160 0.64 -0.1
202,713 - Pf1N1B4_160 0.64 +0.6

Or see this region's nucleotide sequence