Experiment: acetate 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_157 and Pf1N1B4_158 are separated by 37 nucleotides Pf1N1B4_158 and Pf1N1B4_159 are separated by 26 nucleotides Pf1N1B4_159 and Pf1N1B4_160 are separated by 89 nucleotides
Pf1N1B4_157 - ThiJ/PfpI family protein, at 200,059 to 200,745
_157
Pf1N1B4_158 - FIG00456158: hypothetical protein, at 200,783 to 201,112
_158
Pf1N1B4_159 - Putative threonine efflux protein, at 201,139 to 201,768
_159
Pf1N1B4_160 - Transcriptional regulator, GntR family domain / Aspartate aminotransferase (EC 2.6.1.1), at 201,858 to 203,183
_160
Position (kb)
201
202 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 200.345 kb on - strand, within Pf1N1B4_157 at 200.345 kb on - strand, within Pf1N1B4_157 at 200.362 kb on - strand, within Pf1N1B4_157 at 200.599 kb on + strand, within Pf1N1B4_157 at 200.832 kb on + strand, within Pf1N1B4_158 at 200.833 kb on - strand, within Pf1N1B4_158 at 200.833 kb on - strand at 200.833 kb on - strand, within Pf1N1B4_158 at 200.935 kb on + strand, within Pf1N1B4_158 at 200.935 kb on + strand, within Pf1N1B4_158 at 200.956 kb on - strand at 200.959 kb on - strand, within Pf1N1B4_158 at 200.959 kb on - strand, within Pf1N1B4_158 at 200.959 kb on - strand, within Pf1N1B4_158 at 201.090 kb on + strand at 201.091 kb on - strand at 201.095 kb on - strand at 201.108 kb on + strand at 201.108 kb on + strand at 201.108 kb on + strand at 201.108 kb on + strand at 201.108 kb on + strand at 201.109 kb on - strand at 201.109 kb on - strand at 201.231 kb on + strand at 201.255 kb on + strand, within Pf1N1B4_159 at 201.256 kb on - strand, within Pf1N1B4_159 at 201.392 kb on - strand, within Pf1N1B4_159 at 201.665 kb on + strand, within Pf1N1B4_159 at 201.666 kb on - strand, within Pf1N1B4_159 at 201.797 kb on - strand at 201.797 kb on - strand at 201.801 kb on + strand at 201.801 kb on + strand at 201.840 kb on + strand at 202.002 kb on + strand, within Pf1N1B4_160 at 202.003 kb on - strand at 202.168 kb on + strand, within Pf1N1B4_160 at 202.169 kb on - strand, within Pf1N1B4_160 at 202.280 kb on - strand, within Pf1N1B4_160 at 202.280 kb on - strand, within Pf1N1B4_160 at 202.407 kb on + strand, within Pf1N1B4_160 at 202.534 kb on - strand, within Pf1N1B4_160 at 202.647 kb on + strand, within Pf1N1B4_160 at 202.648 kb on - strand, within Pf1N1B4_160 at 202.685 kb on + strand, within Pf1N1B4_160 at 202.685 kb on + strand, within Pf1N1B4_160 at 202.686 kb on - strand, within Pf1N1B4_160 at 202.686 kb on - strand, within Pf1N1B4_160 at 202.710 kb on + strand, within Pf1N1B4_160 at 202.710 kb on + strand, within Pf1N1B4_160 at 202.711 kb on - strand, within Pf1N1B4_160 at 202.711 kb on - strand, within Pf1N1B4_160 at 202.711 kb on - strand, within Pf1N1B4_160 at 202.711 kb on - strand, within Pf1N1B4_160 at 202.711 kb on - strand, within Pf1N1B4_160 at 202.711 kb on - strand, within Pf1N1B4_160 at 202.711 kb on - strand, within Pf1N1B4_160 at 202.711 kb on - strand, within Pf1N1B4_160 at 202.711 kb on - strand at 202.711 kb on - strand, within Pf1N1B4_160 at 202.711 kb on - strand, within Pf1N1B4_160 at 202.713 kb on - strand, within Pf1N1B4_160
Per-strain Table
Position Strand Gene LocusTag Fraction acetate 20 mM remove 200,345 - Pf1N1B4_157 0.42 +0.3 200,345 - Pf1N1B4_157 0.42 +1.1 200,362 - Pf1N1B4_157 0.44 -0.6 200,599 + Pf1N1B4_157 0.79 -1.6 200,832 + Pf1N1B4_158 0.15 -0.3 200,833 - Pf1N1B4_158 0.15 +0.2 200,833 - -0.2 200,833 - Pf1N1B4_158 0.15 -0.6 200,935 + Pf1N1B4_158 0.46 +0.4 200,935 + Pf1N1B4_158 0.46 -2.6 200,956 - +0.8 200,959 - Pf1N1B4_158 0.53 +1.2 200,959 - Pf1N1B4_158 0.53 +0.3 200,959 - Pf1N1B4_158 0.53 -0.0 201,090 + +0.8 201,091 - +1.0 201,095 - +0.8 201,108 + -1.2 201,108 + -0.2 201,108 + -0.1 201,108 + -0.1 201,108 + -0.2 201,109 - +1.8 201,109 - -0.5 201,231 + -0.2 201,255 + Pf1N1B4_159 0.18 -1.0 201,256 - Pf1N1B4_159 0.19 -1.2 201,392 - Pf1N1B4_159 0.40 -2.2 201,665 + Pf1N1B4_159 0.83 +0.3 201,666 - Pf1N1B4_159 0.84 -1.2 201,797 - +1.8 201,797 - -1.8 201,801 + -0.6 201,801 + -0.5 201,840 + -0.8 202,002 + Pf1N1B4_160 0.11 -0.6 202,003 - +0.2 202,168 + Pf1N1B4_160 0.23 -0.8 202,169 - Pf1N1B4_160 0.23 +1.5 202,280 - Pf1N1B4_160 0.32 +0.0 202,280 - Pf1N1B4_160 0.32 +0.5 202,407 + Pf1N1B4_160 0.41 -0.5 202,534 - Pf1N1B4_160 0.51 +0.8 202,647 + Pf1N1B4_160 0.60 +0.8 202,648 - Pf1N1B4_160 0.60 -0.1 202,685 + Pf1N1B4_160 0.62 +0.1 202,685 + Pf1N1B4_160 0.62 +0.2 202,686 - Pf1N1B4_160 0.62 -3.1 202,686 - Pf1N1B4_160 0.62 -1.2 202,710 + Pf1N1B4_160 0.64 -1.0 202,710 + Pf1N1B4_160 0.64 -0.5 202,711 - Pf1N1B4_160 0.64 +0.2 202,711 - Pf1N1B4_160 0.64 +0.0 202,711 - Pf1N1B4_160 0.64 +0.1 202,711 - Pf1N1B4_160 0.64 +1.1 202,711 - Pf1N1B4_160 0.64 -0.2 202,711 - Pf1N1B4_160 0.64 -0.4 202,711 - Pf1N1B4_160 0.64 +2.0 202,711 - Pf1N1B4_160 0.64 -1.6 202,711 - +1.2 202,711 - Pf1N1B4_160 0.64 +0.8 202,711 - Pf1N1B4_160 0.64 -0.1 202,713 - Pf1N1B4_160 0.64 +0.6
Or see this region's nucleotide sequence