Experiment: acetate 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1421 and Pf1N1B4_1422 are separated by 212 nucleotides Pf1N1B4_1422 and Pf1N1B4_1423 are separated by 3 nucleotides Pf1N1B4_1423 and Pf1N1B4_1424 overlap by 1 nucleotides Pf1N1B4_1424 and Pf1N1B4_1425 are separated by 300 nucleotides
Pf1N1B4_1421 - Ribosome small subunit-stimulated GTPase EngC, at 1,629,995 to 1,631,026
_1421
Pf1N1B4_1422 - hypothetical protein, at 1,631,239 to 1,631,646
_1422
Pf1N1B4_1423 - Peptidase C14, caspase catalytic subunit p20, at 1,631,650 to 1,632,609
_1423
Pf1N1B4_1424 - hypothetical protein, at 1,632,609 to 1,633,094
_1424
Pf1N1B4_1425 - hypothetical protein, at 1,633,395 to 1,634,066
_1425
Position (kb)
1631
1632
1633 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1631.021 kb on + strand at 1631.021 kb on + strand at 1631.022 kb on - strand at 1631.024 kb on + strand at 1631.025 kb on - strand at 1631.025 kb on - strand at 1631.025 kb on - strand at 1631.075 kb on - strand at 1631.075 kb on - strand at 1631.075 kb on - strand at 1631.075 kb on - strand at 1631.075 kb on - strand at 1631.075 kb on - strand at 1631.083 kb on - strand at 1631.083 kb on - strand at 1631.083 kb on - strand at 1631.121 kb on - strand at 1631.144 kb on - strand at 1631.144 kb on - strand at 1631.194 kb on + strand at 1631.195 kb on - strand at 1631.195 kb on - strand at 1631.195 kb on - strand at 1631.195 kb on - strand at 1631.195 kb on - strand at 1631.195 kb on - strand at 1631.195 kb on - strand at 1631.195 kb on - strand at 1631.248 kb on + strand at 1631.248 kb on + strand at 1631.249 kb on - strand at 1631.249 kb on - strand at 1631.249 kb on - strand at 1631.458 kb on + strand, within Pf1N1B4_1422 at 1631.458 kb on + strand, within Pf1N1B4_1422 at 1631.458 kb on + strand, within Pf1N1B4_1422 at 1631.458 kb on + strand, within Pf1N1B4_1422 at 1631.458 kb on + strand, within Pf1N1B4_1422 at 1631.458 kb on + strand, within Pf1N1B4_1422 at 1631.458 kb on + strand, within Pf1N1B4_1422 at 1631.459 kb on - strand, within Pf1N1B4_1422 at 1631.459 kb on - strand, within Pf1N1B4_1422 at 1631.459 kb on - strand, within Pf1N1B4_1422 at 1631.459 kb on - strand, within Pf1N1B4_1422 at 1631.467 kb on + strand, within Pf1N1B4_1422 at 1631.468 kb on - strand, within Pf1N1B4_1422 at 1631.468 kb on - strand, within Pf1N1B4_1422 at 1631.468 kb on - strand, within Pf1N1B4_1422 at 1631.676 kb on + strand at 1631.677 kb on - strand at 1631.679 kb on + strand at 1631.679 kb on + strand at 1631.679 kb on + strand at 1631.679 kb on + strand at 1631.680 kb on - strand at 1631.680 kb on - strand at 1631.683 kb on + strand at 1631.683 kb on + strand at 1631.683 kb on + strand at 1631.683 kb on + strand at 1631.683 kb on + strand at 1631.683 kb on + strand at 1631.683 kb on + strand at 1631.683 kb on + strand at 1631.683 kb on + strand at 1631.683 kb on + strand at 1631.683 kb on + strand at 1631.684 kb on - strand at 1631.684 kb on - strand at 1631.684 kb on - strand at 1631.878 kb on + strand, within Pf1N1B4_1423 at 1631.878 kb on + strand, within Pf1N1B4_1423 at 1631.878 kb on + strand, within Pf1N1B4_1423 at 1631.879 kb on - strand, within Pf1N1B4_1423 at 1631.879 kb on - strand, within Pf1N1B4_1423 at 1631.879 kb on - strand, within Pf1N1B4_1423 at 1631.942 kb on - strand, within Pf1N1B4_1423 at 1631.942 kb on - strand, within Pf1N1B4_1423 at 1631.942 kb on - strand, within Pf1N1B4_1423 at 1632.002 kb on + strand, within Pf1N1B4_1423 at 1632.024 kb on + strand, within Pf1N1B4_1423 at 1632.025 kb on - strand, within Pf1N1B4_1423 at 1632.179 kb on + strand, within Pf1N1B4_1423 at 1632.179 kb on + strand, within Pf1N1B4_1423 at 1632.213 kb on - strand, within Pf1N1B4_1423 at 1632.213 kb on - strand, within Pf1N1B4_1423 at 1632.213 kb on - strand, within Pf1N1B4_1423 at 1632.213 kb on - strand, within Pf1N1B4_1423 at 1632.241 kb on + strand, within Pf1N1B4_1423 at 1632.242 kb on - strand, within Pf1N1B4_1423 at 1632.242 kb on - strand, within Pf1N1B4_1423 at 1632.341 kb on + strand, within Pf1N1B4_1423 at 1632.342 kb on - strand, within Pf1N1B4_1423 at 1632.415 kb on + strand, within Pf1N1B4_1423 at 1632.416 kb on - strand, within Pf1N1B4_1423 at 1632.523 kb on + strand at 1632.523 kb on + strand at 1632.523 kb on + strand at 1632.523 kb on + strand at 1632.523 kb on + strand at 1632.523 kb on + strand at 1632.523 kb on + strand at 1632.524 kb on - strand at 1632.524 kb on - strand at 1632.524 kb on - strand at 1632.524 kb on - strand at 1632.524 kb on - strand at 1632.524 kb on - strand at 1632.524 kb on - strand at 1632.524 kb on - strand at 1632.524 kb on - strand at 1632.524 kb on - strand at 1632.524 kb on - strand at 1632.524 kb on - strand at 1632.524 kb on - strand at 1632.524 kb on - strand at 1632.524 kb on - strand at 1632.524 kb on - strand at 1632.526 kb on + strand at 1632.526 kb on + strand at 1632.527 kb on - strand at 1632.527 kb on - strand at 1632.527 kb on - strand at 1632.574 kb on + strand at 1632.575 kb on - strand at 1632.575 kb on - strand at 1632.575 kb on - strand at 1632.578 kb on - strand at 1632.620 kb on + strand at 1632.620 kb on + strand at 1632.620 kb on + strand at 1632.621 kb on - strand at 1632.621 kb on - strand at 1632.627 kb on + strand at 1632.696 kb on + strand, within Pf1N1B4_1424 at 1632.696 kb on + strand, within Pf1N1B4_1424 at 1632.696 kb on + strand, within Pf1N1B4_1424 at 1632.697 kb on - strand, within Pf1N1B4_1424 at 1632.697 kb on - strand, within Pf1N1B4_1424 at 1632.697 kb on - strand, within Pf1N1B4_1424 at 1632.697 kb on - strand, within Pf1N1B4_1424 at 1632.697 kb on - strand, within Pf1N1B4_1424 at 1632.866 kb on - strand, within Pf1N1B4_1424 at 1632.904 kb on - strand, within Pf1N1B4_1424 at 1632.911 kb on + strand, within Pf1N1B4_1424 at 1633.032 kb on + strand, within Pf1N1B4_1424 at 1633.032 kb on + strand, within Pf1N1B4_1424 at 1633.032 kb on + strand, within Pf1N1B4_1424 at 1633.033 kb on - strand, within Pf1N1B4_1424 at 1633.033 kb on - strand, within Pf1N1B4_1424 at 1633.135 kb on + strand at 1633.135 kb on + strand at 1633.136 kb on - strand at 1633.136 kb on - strand at 1633.159 kb on + strand at 1633.159 kb on + strand at 1633.160 kb on - strand at 1633.212 kb on + strand at 1633.318 kb on + strand at 1633.318 kb on + strand at 1633.318 kb on + strand at 1633.318 kb on + strand at 1633.319 kb on - strand at 1633.319 kb on - strand at 1633.319 kb on - strand at 1633.319 kb on - strand at 1633.319 kb on - strand at 1633.319 kb on - strand at 1633.319 kb on - strand at 1633.330 kb on + strand at 1633.366 kb on + strand at 1633.370 kb on - strand at 1633.553 kb on + strand, within Pf1N1B4_1425 at 1633.554 kb on - strand, within Pf1N1B4_1425 at 1633.569 kb on + strand, within Pf1N1B4_1425 at 1633.569 kb on + strand, within Pf1N1B4_1425 at 1633.569 kb on + strand, within Pf1N1B4_1425 at 1633.570 kb on - strand, within Pf1N1B4_1425 at 1633.570 kb on - strand, within Pf1N1B4_1425 at 1633.570 kb on - strand, within Pf1N1B4_1425 at 1633.570 kb on - strand, within Pf1N1B4_1425
Per-strain Table
Position Strand Gene LocusTag Fraction acetate 20 mM remove 1,631,021 + +0.5 1,631,021 + -0.3 1,631,022 - -1.9 1,631,024 + +1.3 1,631,025 - +0.7 1,631,025 - +0.7 1,631,025 - +0.6 1,631,075 - -0.7 1,631,075 - +1.0 1,631,075 - -0.7 1,631,075 - -0.3 1,631,075 - -0.0 1,631,075 - +0.7 1,631,083 - +1.3 1,631,083 - +0.1 1,631,083 - +0.3 1,631,121 - -0.0 1,631,144 - -0.7 1,631,144 - -0.9 1,631,194 + -0.5 1,631,195 - -0.1 1,631,195 - +0.5 1,631,195 - +0.1 1,631,195 - -0.5 1,631,195 - -0.0 1,631,195 - +0.7 1,631,195 - +3.3 1,631,195 - +1.3 1,631,248 + +1.6 1,631,248 + -0.3 1,631,249 - +1.3 1,631,249 - -1.0 1,631,249 - -0.4 1,631,458 + Pf1N1B4_1422 0.54 +0.7 1,631,458 + Pf1N1B4_1422 0.54 +0.9 1,631,458 + Pf1N1B4_1422 0.54 -0.7 1,631,458 + Pf1N1B4_1422 0.54 +2.6 1,631,458 + Pf1N1B4_1422 0.54 -0.3 1,631,458 + Pf1N1B4_1422 0.54 +1.3 1,631,458 + Pf1N1B4_1422 0.54 +1.0 1,631,459 - Pf1N1B4_1422 0.54 +2.0 1,631,459 - Pf1N1B4_1422 0.54 +3.6 1,631,459 - Pf1N1B4_1422 0.54 -0.7 1,631,459 - Pf1N1B4_1422 0.54 +0.7 1,631,467 + Pf1N1B4_1422 0.56 -1.0 1,631,468 - Pf1N1B4_1422 0.56 +0.5 1,631,468 - Pf1N1B4_1422 0.56 +0.3 1,631,468 - Pf1N1B4_1422 0.56 +2.0 1,631,676 + -0.2 1,631,677 - -0.5 1,631,679 + +0.3 1,631,679 + -0.7 1,631,679 + +1.3 1,631,679 + -0.6 1,631,680 - +0.7 1,631,680 - +2.3 1,631,683 + -0.7 1,631,683 + +2.1 1,631,683 + +0.4 1,631,683 + -0.5 1,631,683 + -0.7 1,631,683 + +0.4 1,631,683 + -1.0 1,631,683 + -0.7 1,631,683 + -0.5 1,631,683 + +0.4 1,631,683 + -1.3 1,631,684 - +1.3 1,631,684 - -2.0 1,631,684 - +0.5 1,631,878 + Pf1N1B4_1423 0.24 +2.3 1,631,878 + Pf1N1B4_1423 0.24 +1.2 1,631,878 + Pf1N1B4_1423 0.24 +1.9 1,631,879 - Pf1N1B4_1423 0.24 -0.7 1,631,879 - Pf1N1B4_1423 0.24 +0.5 1,631,879 - Pf1N1B4_1423 0.24 +2.1 1,631,942 - Pf1N1B4_1423 0.30 +0.7 1,631,942 - Pf1N1B4_1423 0.30 +1.4 1,631,942 - Pf1N1B4_1423 0.30 -0.6 1,632,002 + Pf1N1B4_1423 0.37 +0.1 1,632,024 + Pf1N1B4_1423 0.39 -0.5 1,632,025 - Pf1N1B4_1423 0.39 +2.0 1,632,179 + Pf1N1B4_1423 0.55 -0.5 1,632,179 + Pf1N1B4_1423 0.55 -0.1 1,632,213 - Pf1N1B4_1423 0.59 -0.1 1,632,213 - Pf1N1B4_1423 0.59 -0.5 1,632,213 - Pf1N1B4_1423 0.59 +0.0 1,632,213 - Pf1N1B4_1423 0.59 +0.9 1,632,241 + Pf1N1B4_1423 0.62 +0.6 1,632,242 - Pf1N1B4_1423 0.62 +0.2 1,632,242 - Pf1N1B4_1423 0.62 +0.5 1,632,341 + Pf1N1B4_1423 0.72 -0.7 1,632,342 - Pf1N1B4_1423 0.72 -1.7 1,632,415 + Pf1N1B4_1423 0.80 -1.5 1,632,416 - Pf1N1B4_1423 0.80 +0.8 1,632,523 + -0.3 1,632,523 + -0.0 1,632,523 + -0.0 1,632,523 + +0.8 1,632,523 + -1.0 1,632,523 + +1.5 1,632,523 + +0.3 1,632,524 - +0.8 1,632,524 - -1.6 1,632,524 - +1.7 1,632,524 - +0.9 1,632,524 - +1.7 1,632,524 - +1.6 1,632,524 - +0.3 1,632,524 - +1.9 1,632,524 - -0.0 1,632,524 - -0.3 1,632,524 - +1.0 1,632,524 - -0.9 1,632,524 - +1.0 1,632,524 - +2.0 1,632,524 - +0.8 1,632,524 - +0.6 1,632,526 + -0.9 1,632,526 + +3.3 1,632,527 - -1.0 1,632,527 - +0.8 1,632,527 - +0.4 1,632,574 + -0.7 1,632,575 - +2.0 1,632,575 - -1.0 1,632,575 - +0.3 1,632,578 - +0.1 1,632,620 + -0.6 1,632,620 + -1.0 1,632,620 + +0.9 1,632,621 - +1.6 1,632,621 - +1.3 1,632,627 + +0.4 1,632,696 + Pf1N1B4_1424 0.18 -0.3 1,632,696 + Pf1N1B4_1424 0.18 +1.3 1,632,696 + Pf1N1B4_1424 0.18 +0.5 1,632,697 - Pf1N1B4_1424 0.18 -1.3 1,632,697 - Pf1N1B4_1424 0.18 -1.5 1,632,697 - Pf1N1B4_1424 0.18 +1.2 1,632,697 - Pf1N1B4_1424 0.18 -0.3 1,632,697 - Pf1N1B4_1424 0.18 +2.3 1,632,866 - Pf1N1B4_1424 0.53 +0.5 1,632,904 - Pf1N1B4_1424 0.61 +0.9 1,632,911 + Pf1N1B4_1424 0.62 +0.1 1,633,032 + Pf1N1B4_1424 0.87 -1.0 1,633,032 + Pf1N1B4_1424 0.87 -0.6 1,633,032 + Pf1N1B4_1424 0.87 +0.6 1,633,033 - Pf1N1B4_1424 0.87 -0.1 1,633,033 - Pf1N1B4_1424 0.87 -0.2 1,633,135 + -0.7 1,633,135 + -0.1 1,633,136 - +0.0 1,633,136 - -0.5 1,633,159 + -0.5 1,633,159 + +0.3 1,633,160 - -0.5 1,633,212 + -2.2 1,633,318 + +1.3 1,633,318 + +0.1 1,633,318 + +0.1 1,633,318 + +1.0 1,633,319 - +1.3 1,633,319 - -1.0 1,633,319 - +0.0 1,633,319 - -0.3 1,633,319 - +0.5 1,633,319 - +0.7 1,633,319 - -1.3 1,633,330 + +1.3 1,633,366 + +0.1 1,633,370 - +0.3 1,633,553 + Pf1N1B4_1425 0.24 +0.9 1,633,554 - Pf1N1B4_1425 0.24 +0.1 1,633,569 + Pf1N1B4_1425 0.26 +0.3 1,633,569 + Pf1N1B4_1425 0.26 -0.2 1,633,569 + Pf1N1B4_1425 0.26 +1.7 1,633,570 - Pf1N1B4_1425 0.26 +0.1 1,633,570 - Pf1N1B4_1425 0.26 +0.2 1,633,570 - Pf1N1B4_1425 0.26 -0.0 1,633,570 - Pf1N1B4_1425 0.26 -0.7
Or see this region's nucleotide sequence