Experiment: acetate 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1349 and Pf1N1B4_1350 are separated by 9 nucleotides Pf1N1B4_1350 and Pf1N1B4_1351 are separated by 5 nucleotides Pf1N1B4_1351 and Pf1N1B4_1352 overlap by 1 nucleotides Pf1N1B4_1352 and Pf1N1B4_1353 are separated by 126 nucleotides
Pf1N1B4_1349 - Urease gamma subunit (EC 3.5.1.5), at 1,546,092 to 1,546,394
_1349
Pf1N1B4_1350 - GCN5-related N-acetyltransferase, at 1,546,404 to 1,546,937
_1350
Pf1N1B4_1351 - Tabtoxin resistance protein, at 1,546,943 to 1,547,476
_1351
Pf1N1B4_1352 - Urease beta subunit (EC 3.5.1.5), at 1,547,476 to 1,547,784
_1352
Pf1N1B4_1353 - Urease alpha subunit (EC 3.5.1.5), at 1,547,911 to 1,549,611
_1353
Position (kb)
1546
1547
1548 Strain fitness (log2 ratio)
-1
0
1
2 at 1545.978 kb on - strand at 1545.978 kb on - strand at 1546.047 kb on - strand at 1546.067 kb on - strand at 1546.204 kb on - strand, within Pf1N1B4_1349 at 1546.410 kb on + strand at 1546.410 kb on + strand at 1546.411 kb on - strand at 1546.461 kb on + strand, within Pf1N1B4_1350 at 1546.461 kb on + strand, within Pf1N1B4_1350 at 1546.461 kb on + strand, within Pf1N1B4_1350 at 1546.461 kb on + strand, within Pf1N1B4_1350 at 1546.462 kb on - strand, within Pf1N1B4_1350 at 1546.598 kb on - strand, within Pf1N1B4_1350 at 1546.668 kb on + strand, within Pf1N1B4_1350 at 1546.669 kb on - strand, within Pf1N1B4_1350 at 1546.849 kb on + strand, within Pf1N1B4_1350 at 1546.939 kb on - strand at 1547.087 kb on + strand, within Pf1N1B4_1351 at 1547.088 kb on - strand, within Pf1N1B4_1351 at 1547.324 kb on + strand, within Pf1N1B4_1351 at 1547.324 kb on + strand, within Pf1N1B4_1351 at 1547.324 kb on + strand, within Pf1N1B4_1351 at 1547.325 kb on - strand, within Pf1N1B4_1351 at 1547.364 kb on - strand, within Pf1N1B4_1351 at 1547.364 kb on - strand, within Pf1N1B4_1351 at 1547.364 kb on - strand, within Pf1N1B4_1351 at 1547.364 kb on - strand at 1547.448 kb on - strand at 1547.450 kb on + strand at 1547.450 kb on + strand at 1547.450 kb on + strand at 1547.451 kb on - strand at 1547.451 kb on - strand at 1547.451 kb on - strand at 1547.451 kb on - strand at 1547.593 kb on + strand, within Pf1N1B4_1352 at 1547.782 kb on + strand at 1547.933 kb on - strand at 1548.023 kb on - strand at 1548.309 kb on + strand, within Pf1N1B4_1353
Per-strain Table
Position Strand Gene LocusTag Fraction acetate 20 mM remove 1,545,978 - +0.2 1,545,978 - +0.5 1,546,047 - +0.3 1,546,067 - +2.1 1,546,204 - Pf1N1B4_1349 0.37 -1.1 1,546,410 + +0.7 1,546,410 + -0.6 1,546,411 - +2.3 1,546,461 + Pf1N1B4_1350 0.11 +0.3 1,546,461 + Pf1N1B4_1350 0.11 -1.1 1,546,461 + Pf1N1B4_1350 0.11 +2.0 1,546,461 + Pf1N1B4_1350 0.11 +0.7 1,546,462 - Pf1N1B4_1350 0.11 +0.4 1,546,598 - Pf1N1B4_1350 0.36 +0.8 1,546,668 + Pf1N1B4_1350 0.49 +0.1 1,546,669 - Pf1N1B4_1350 0.50 +0.4 1,546,849 + Pf1N1B4_1350 0.83 +0.6 1,546,939 - -0.1 1,547,087 + Pf1N1B4_1351 0.27 -0.2 1,547,088 - Pf1N1B4_1351 0.27 +0.7 1,547,324 + Pf1N1B4_1351 0.71 +0.3 1,547,324 + Pf1N1B4_1351 0.71 +0.7 1,547,324 + Pf1N1B4_1351 0.71 +0.3 1,547,325 - Pf1N1B4_1351 0.72 -1.3 1,547,364 - Pf1N1B4_1351 0.79 -1.1 1,547,364 - Pf1N1B4_1351 0.79 -0.3 1,547,364 - Pf1N1B4_1351 0.79 +0.5 1,547,364 - +1.8 1,547,448 - +0.6 1,547,450 + +1.3 1,547,450 + -0.7 1,547,450 + +1.8 1,547,451 - +0.3 1,547,451 - +0.7 1,547,451 - -0.9 1,547,451 - -0.7 1,547,593 + Pf1N1B4_1352 0.38 +0.6 1,547,782 + -0.5 1,547,933 - +0.0 1,548,023 - -0.4 1,548,309 + Pf1N1B4_1353 0.23 +0.7
Or see this region's nucleotide sequence