Experiment: acetate 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1019 and Pf1N1B4_1020 overlap by 8 nucleotides Pf1N1B4_1020 and Pf1N1B4_1021 are separated by 204 nucleotides
Pf1N1B4_1019 - Branched-chain alpha-keto acid dehydrogenase, E1 component, beta subunit (EC 1.2.4.4), at 1,154,425 to 1,155,414
_1019
Pf1N1B4_1020 - Branched-chain alpha-keto acid dehydrogenase, E1 component, alpha subunit (EC 1.2.4.4), at 1,155,407 to 1,156,405
_1020
Pf1N1B4_1021 - Leucine dehydrogenase (EC 1.4.1.9), at 1,156,610 to 1,157,629
_1021
Position (kb)
1155
1156
1157 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1154.432 kb on + strand at 1154.433 kb on - strand at 1154.486 kb on + strand at 1154.486 kb on + strand at 1154.486 kb on + strand at 1154.486 kb on + strand at 1154.486 kb on + strand at 1154.486 kb on + strand at 1154.486 kb on + strand at 1154.486 kb on + strand at 1154.486 kb on + strand at 1154.486 kb on + strand at 1154.486 kb on + strand at 1154.486 kb on + strand at 1154.487 kb on - strand at 1154.487 kb on - strand at 1154.487 kb on - strand at 1154.487 kb on - strand at 1154.487 kb on - strand at 1154.504 kb on + strand at 1154.577 kb on - strand, within Pf1N1B4_1019 at 1154.793 kb on + strand, within Pf1N1B4_1019 at 1154.793 kb on + strand, within Pf1N1B4_1019 at 1154.793 kb on + strand, within Pf1N1B4_1019 at 1154.794 kb on - strand, within Pf1N1B4_1019 at 1154.794 kb on - strand, within Pf1N1B4_1019 at 1154.794 kb on - strand, within Pf1N1B4_1019 at 1154.880 kb on - strand, within Pf1N1B4_1019 at 1154.902 kb on + strand, within Pf1N1B4_1019 at 1154.902 kb on + strand, within Pf1N1B4_1019 at 1154.903 kb on - strand, within Pf1N1B4_1019 at 1154.903 kb on - strand, within Pf1N1B4_1019 at 1155.146 kb on + strand, within Pf1N1B4_1019 at 1155.146 kb on + strand, within Pf1N1B4_1019 at 1155.146 kb on + strand, within Pf1N1B4_1019 at 1155.146 kb on + strand, within Pf1N1B4_1019 at 1155.147 kb on - strand, within Pf1N1B4_1019 at 1155.147 kb on - strand, within Pf1N1B4_1019 at 1155.147 kb on - strand at 1155.147 kb on - strand, within Pf1N1B4_1019 at 1155.311 kb on - strand, within Pf1N1B4_1019 at 1155.495 kb on + strand at 1155.495 kb on + strand at 1155.495 kb on + strand at 1155.588 kb on - strand, within Pf1N1B4_1020 at 1155.631 kb on + strand, within Pf1N1B4_1020 at 1156.157 kb on - strand, within Pf1N1B4_1020 at 1156.179 kb on + strand, within Pf1N1B4_1020 at 1156.179 kb on + strand, within Pf1N1B4_1020 at 1156.180 kb on - strand, within Pf1N1B4_1020 at 1156.180 kb on - strand, within Pf1N1B4_1020 at 1156.180 kb on - strand, within Pf1N1B4_1020 at 1156.239 kb on + strand, within Pf1N1B4_1020 at 1156.240 kb on - strand, within Pf1N1B4_1020 at 1156.250 kb on + strand, within Pf1N1B4_1020 at 1156.250 kb on + strand, within Pf1N1B4_1020 at 1156.250 kb on + strand, within Pf1N1B4_1020 at 1156.250 kb on + strand, within Pf1N1B4_1020 at 1156.251 kb on - strand, within Pf1N1B4_1020 at 1156.251 kb on - strand at 1156.251 kb on - strand, within Pf1N1B4_1020 at 1156.475 kb on + strand at 1156.475 kb on + strand at 1156.618 kb on - strand at 1156.618 kb on - strand at 1156.770 kb on + strand, within Pf1N1B4_1021 at 1156.770 kb on + strand, within Pf1N1B4_1021 at 1156.770 kb on + strand, within Pf1N1B4_1021 at 1156.770 kb on + strand, within Pf1N1B4_1021 at 1156.770 kb on + strand, within Pf1N1B4_1021 at 1156.770 kb on + strand, within Pf1N1B4_1021 at 1156.770 kb on + strand, within Pf1N1B4_1021 at 1156.770 kb on + strand, within Pf1N1B4_1021 at 1156.770 kb on + strand, within Pf1N1B4_1021 at 1156.771 kb on - strand, within Pf1N1B4_1021 at 1156.771 kb on - strand, within Pf1N1B4_1021 at 1156.771 kb on - strand, within Pf1N1B4_1021 at 1156.771 kb on - strand, within Pf1N1B4_1021 at 1156.771 kb on - strand, within Pf1N1B4_1021 at 1156.771 kb on - strand, within Pf1N1B4_1021 at 1156.771 kb on - strand, within Pf1N1B4_1021 at 1156.771 kb on - strand, within Pf1N1B4_1021 at 1156.771 kb on - strand, within Pf1N1B4_1021 at 1156.797 kb on + strand, within Pf1N1B4_1021 at 1156.797 kb on + strand, within Pf1N1B4_1021 at 1156.809 kb on + strand, within Pf1N1B4_1021 at 1156.810 kb on - strand, within Pf1N1B4_1021 at 1156.918 kb on + strand, within Pf1N1B4_1021 at 1156.918 kb on + strand, within Pf1N1B4_1021 at 1156.949 kb on + strand, within Pf1N1B4_1021 at 1156.949 kb on + strand, within Pf1N1B4_1021 at 1156.949 kb on + strand, within Pf1N1B4_1021 at 1156.949 kb on + strand, within Pf1N1B4_1021 at 1156.949 kb on + strand, within Pf1N1B4_1021 at 1156.950 kb on - strand, within Pf1N1B4_1021 at 1156.950 kb on - strand, within Pf1N1B4_1021 at 1157.072 kb on + strand, within Pf1N1B4_1021 at 1157.072 kb on + strand, within Pf1N1B4_1021 at 1157.072 kb on + strand, within Pf1N1B4_1021 at 1157.072 kb on + strand, within Pf1N1B4_1021 at 1157.141 kb on - strand, within Pf1N1B4_1021 at 1157.307 kb on + strand, within Pf1N1B4_1021 at 1157.307 kb on + strand, within Pf1N1B4_1021 at 1157.307 kb on + strand, within Pf1N1B4_1021 at 1157.308 kb on - strand, within Pf1N1B4_1021 at 1157.308 kb on - strand, within Pf1N1B4_1021 at 1157.308 kb on - strand, within Pf1N1B4_1021 at 1157.352 kb on - strand, within Pf1N1B4_1021 at 1157.352 kb on - strand, within Pf1N1B4_1021
Per-strain Table
Position Strand Gene LocusTag Fraction acetate 20 mM remove 1,154,432 + +0.2 1,154,433 - +0.2 1,154,486 + -1.7 1,154,486 + -1.2 1,154,486 + -0.7 1,154,486 + -1.2 1,154,486 + -0.3 1,154,486 + +1.9 1,154,486 + +0.0 1,154,486 + +1.5 1,154,486 + +0.2 1,154,486 + -0.3 1,154,486 + +0.0 1,154,486 + -0.1 1,154,487 - -0.7 1,154,487 - +0.9 1,154,487 - -0.7 1,154,487 - +0.3 1,154,487 - -0.8 1,154,504 + -0.2 1,154,577 - Pf1N1B4_1019 0.15 -0.8 1,154,793 + Pf1N1B4_1019 0.37 +0.2 1,154,793 + Pf1N1B4_1019 0.37 -0.7 1,154,793 + Pf1N1B4_1019 0.37 -1.2 1,154,794 - Pf1N1B4_1019 0.37 -1.2 1,154,794 - Pf1N1B4_1019 0.37 +0.9 1,154,794 - Pf1N1B4_1019 0.37 +0.2 1,154,880 - Pf1N1B4_1019 0.46 +0.2 1,154,902 + Pf1N1B4_1019 0.48 -0.4 1,154,902 + Pf1N1B4_1019 0.48 +0.6 1,154,903 - Pf1N1B4_1019 0.48 +1.2 1,154,903 - Pf1N1B4_1019 0.48 +1.2 1,155,146 + Pf1N1B4_1019 0.73 -0.8 1,155,146 + Pf1N1B4_1019 0.73 +1.0 1,155,146 + Pf1N1B4_1019 0.73 -0.2 1,155,146 + Pf1N1B4_1019 0.73 +0.2 1,155,147 - Pf1N1B4_1019 0.73 -0.8 1,155,147 - Pf1N1B4_1019 0.73 +0.9 1,155,147 - +1.2 1,155,147 - Pf1N1B4_1019 0.73 +0.3 1,155,311 - Pf1N1B4_1019 0.89 -0.6 1,155,495 + +0.2 1,155,495 + -1.7 1,155,495 + +1.2 1,155,588 - Pf1N1B4_1020 0.18 +0.9 1,155,631 + Pf1N1B4_1020 0.22 +1.8 1,156,157 - Pf1N1B4_1020 0.75 +0.9 1,156,179 + Pf1N1B4_1020 0.77 -0.3 1,156,179 + Pf1N1B4_1020 0.77 +0.8 1,156,180 - Pf1N1B4_1020 0.77 -1.2 1,156,180 - Pf1N1B4_1020 0.77 +0.2 1,156,180 - Pf1N1B4_1020 0.77 -1.5 1,156,239 + Pf1N1B4_1020 0.83 +0.4 1,156,240 - Pf1N1B4_1020 0.83 -0.4 1,156,250 + Pf1N1B4_1020 0.84 -1.7 1,156,250 + Pf1N1B4_1020 0.84 +0.3 1,156,250 + Pf1N1B4_1020 0.84 +0.0 1,156,250 + Pf1N1B4_1020 0.84 -1.8 1,156,251 - Pf1N1B4_1020 0.84 -1.3 1,156,251 - +1.2 1,156,251 - Pf1N1B4_1020 0.84 -1.2 1,156,475 + +0.6 1,156,475 + -0.7 1,156,618 - -0.3 1,156,618 - -0.0 1,156,770 + Pf1N1B4_1021 0.16 -0.1 1,156,770 + Pf1N1B4_1021 0.16 +0.6 1,156,770 + Pf1N1B4_1021 0.16 +1.2 1,156,770 + Pf1N1B4_1021 0.16 -1.7 1,156,770 + Pf1N1B4_1021 0.16 -0.3 1,156,770 + Pf1N1B4_1021 0.16 -0.4 1,156,770 + Pf1N1B4_1021 0.16 -0.7 1,156,770 + Pf1N1B4_1021 0.16 +0.9 1,156,770 + Pf1N1B4_1021 0.16 -0.2 1,156,771 - Pf1N1B4_1021 0.16 -0.3 1,156,771 - Pf1N1B4_1021 0.16 -1.0 1,156,771 - Pf1N1B4_1021 0.16 -1.8 1,156,771 - Pf1N1B4_1021 0.16 -1.0 1,156,771 - Pf1N1B4_1021 0.16 +0.8 1,156,771 - Pf1N1B4_1021 0.16 +0.2 1,156,771 - Pf1N1B4_1021 0.16 +1.4 1,156,771 - Pf1N1B4_1021 0.16 -0.8 1,156,771 - Pf1N1B4_1021 0.16 +0.6 1,156,797 + Pf1N1B4_1021 0.18 -1.2 1,156,797 + Pf1N1B4_1021 0.18 -1.0 1,156,809 + Pf1N1B4_1021 0.20 -0.4 1,156,810 - Pf1N1B4_1021 0.20 +0.4 1,156,918 + Pf1N1B4_1021 0.30 +0.4 1,156,918 + Pf1N1B4_1021 0.30 +0.3 1,156,949 + Pf1N1B4_1021 0.33 -0.0 1,156,949 + Pf1N1B4_1021 0.33 -0.4 1,156,949 + Pf1N1B4_1021 0.33 -0.4 1,156,949 + Pf1N1B4_1021 0.33 -0.2 1,156,949 + Pf1N1B4_1021 0.33 +0.7 1,156,950 - Pf1N1B4_1021 0.33 +1.6 1,156,950 - Pf1N1B4_1021 0.33 -0.3 1,157,072 + Pf1N1B4_1021 0.45 +1.2 1,157,072 + Pf1N1B4_1021 0.45 -1.3 1,157,072 + Pf1N1B4_1021 0.45 -0.5 1,157,072 + Pf1N1B4_1021 0.45 -0.2 1,157,141 - Pf1N1B4_1021 0.52 -2.8 1,157,307 + Pf1N1B4_1021 0.68 -0.1 1,157,307 + Pf1N1B4_1021 0.68 +0.9 1,157,307 + Pf1N1B4_1021 0.68 +0.6 1,157,308 - Pf1N1B4_1021 0.68 -1.2 1,157,308 - Pf1N1B4_1021 0.68 +0.9 1,157,308 - Pf1N1B4_1021 0.68 -0.8 1,157,352 - Pf1N1B4_1021 0.73 +0.2 1,157,352 - Pf1N1B4_1021 0.73 -0.4
Or see this region's nucleotide sequence