Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_1020

Experiment: acetate 20 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_1019 and Pf1N1B4_1020 overlap by 8 nucleotidesPf1N1B4_1020 and Pf1N1B4_1021 are separated by 204 nucleotides Pf1N1B4_1019 - Branched-chain alpha-keto acid dehydrogenase, E1 component, beta subunit (EC 1.2.4.4), at 1,154,425 to 1,155,414 _1019 Pf1N1B4_1020 - Branched-chain alpha-keto acid dehydrogenase, E1 component, alpha subunit (EC 1.2.4.4), at 1,155,407 to 1,156,405 _1020 Pf1N1B4_1021 - Leucine dehydrogenase (EC 1.4.1.9), at 1,156,610 to 1,157,629 _1021 Position (kb) 1155 1156 1157Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 1154.432 kb on + strandat 1154.433 kb on - strandat 1154.486 kb on + strandat 1154.486 kb on + strandat 1154.486 kb on + strandat 1154.486 kb on + strandat 1154.486 kb on + strandat 1154.486 kb on + strandat 1154.486 kb on + strandat 1154.486 kb on + strandat 1154.486 kb on + strandat 1154.486 kb on + strandat 1154.486 kb on + strandat 1154.486 kb on + strandat 1154.487 kb on - strandat 1154.487 kb on - strandat 1154.487 kb on - strandat 1154.487 kb on - strandat 1154.487 kb on - strandat 1154.504 kb on + strandat 1154.577 kb on - strand, within Pf1N1B4_1019at 1154.793 kb on + strand, within Pf1N1B4_1019at 1154.793 kb on + strand, within Pf1N1B4_1019at 1154.793 kb on + strand, within Pf1N1B4_1019at 1154.794 kb on - strand, within Pf1N1B4_1019at 1154.794 kb on - strand, within Pf1N1B4_1019at 1154.794 kb on - strand, within Pf1N1B4_1019at 1154.880 kb on - strand, within Pf1N1B4_1019at 1154.902 kb on + strand, within Pf1N1B4_1019at 1154.902 kb on + strand, within Pf1N1B4_1019at 1154.903 kb on - strand, within Pf1N1B4_1019at 1154.903 kb on - strand, within Pf1N1B4_1019at 1155.146 kb on + strand, within Pf1N1B4_1019at 1155.146 kb on + strand, within Pf1N1B4_1019at 1155.146 kb on + strand, within Pf1N1B4_1019at 1155.146 kb on + strand, within Pf1N1B4_1019at 1155.147 kb on - strand, within Pf1N1B4_1019at 1155.147 kb on - strand, within Pf1N1B4_1019at 1155.147 kb on - strandat 1155.147 kb on - strand, within Pf1N1B4_1019at 1155.311 kb on - strand, within Pf1N1B4_1019at 1155.495 kb on + strandat 1155.495 kb on + strandat 1155.495 kb on + strandat 1155.588 kb on - strand, within Pf1N1B4_1020at 1155.631 kb on + strand, within Pf1N1B4_1020at 1156.157 kb on - strand, within Pf1N1B4_1020at 1156.179 kb on + strand, within Pf1N1B4_1020at 1156.179 kb on + strand, within Pf1N1B4_1020at 1156.180 kb on - strand, within Pf1N1B4_1020at 1156.180 kb on - strand, within Pf1N1B4_1020at 1156.180 kb on - strand, within Pf1N1B4_1020at 1156.239 kb on + strand, within Pf1N1B4_1020at 1156.240 kb on - strand, within Pf1N1B4_1020at 1156.250 kb on + strand, within Pf1N1B4_1020at 1156.250 kb on + strand, within Pf1N1B4_1020at 1156.250 kb on + strand, within Pf1N1B4_1020at 1156.250 kb on + strand, within Pf1N1B4_1020at 1156.251 kb on - strand, within Pf1N1B4_1020at 1156.251 kb on - strandat 1156.251 kb on - strand, within Pf1N1B4_1020at 1156.475 kb on + strandat 1156.475 kb on + strandat 1156.618 kb on - strandat 1156.618 kb on - strandat 1156.770 kb on + strand, within Pf1N1B4_1021at 1156.770 kb on + strand, within Pf1N1B4_1021at 1156.770 kb on + strand, within Pf1N1B4_1021at 1156.770 kb on + strand, within Pf1N1B4_1021at 1156.770 kb on + strand, within Pf1N1B4_1021at 1156.770 kb on + strand, within Pf1N1B4_1021at 1156.770 kb on + strand, within Pf1N1B4_1021at 1156.770 kb on + strand, within Pf1N1B4_1021at 1156.770 kb on + strand, within Pf1N1B4_1021at 1156.771 kb on - strand, within Pf1N1B4_1021at 1156.771 kb on - strand, within Pf1N1B4_1021at 1156.771 kb on - strand, within Pf1N1B4_1021at 1156.771 kb on - strand, within Pf1N1B4_1021at 1156.771 kb on - strand, within Pf1N1B4_1021at 1156.771 kb on - strand, within Pf1N1B4_1021at 1156.771 kb on - strand, within Pf1N1B4_1021at 1156.771 kb on - strand, within Pf1N1B4_1021at 1156.771 kb on - strand, within Pf1N1B4_1021at 1156.797 kb on + strand, within Pf1N1B4_1021at 1156.797 kb on + strand, within Pf1N1B4_1021at 1156.809 kb on + strand, within Pf1N1B4_1021at 1156.810 kb on - strand, within Pf1N1B4_1021at 1156.918 kb on + strand, within Pf1N1B4_1021at 1156.918 kb on + strand, within Pf1N1B4_1021at 1156.949 kb on + strand, within Pf1N1B4_1021at 1156.949 kb on + strand, within Pf1N1B4_1021at 1156.949 kb on + strand, within Pf1N1B4_1021at 1156.949 kb on + strand, within Pf1N1B4_1021at 1156.949 kb on + strand, within Pf1N1B4_1021at 1156.950 kb on - strand, within Pf1N1B4_1021at 1156.950 kb on - strand, within Pf1N1B4_1021at 1157.072 kb on + strand, within Pf1N1B4_1021at 1157.072 kb on + strand, within Pf1N1B4_1021at 1157.072 kb on + strand, within Pf1N1B4_1021at 1157.072 kb on + strand, within Pf1N1B4_1021at 1157.141 kb on - strand, within Pf1N1B4_1021at 1157.307 kb on + strand, within Pf1N1B4_1021at 1157.307 kb on + strand, within Pf1N1B4_1021at 1157.307 kb on + strand, within Pf1N1B4_1021at 1157.308 kb on - strand, within Pf1N1B4_1021at 1157.308 kb on - strand, within Pf1N1B4_1021at 1157.308 kb on - strand, within Pf1N1B4_1021at 1157.352 kb on - strand, within Pf1N1B4_1021at 1157.352 kb on - strand, within Pf1N1B4_1021

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Per-strain Table

Position Strand Gene LocusTag Fraction acetate 20 mM
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1,154,432 + +0.2
1,154,433 - +0.2
1,154,486 + -1.7
1,154,486 + -1.2
1,154,486 + -0.7
1,154,486 + -1.2
1,154,486 + -0.3
1,154,486 + +1.9
1,154,486 + +0.0
1,154,486 + +1.5
1,154,486 + +0.2
1,154,486 + -0.3
1,154,486 + +0.0
1,154,486 + -0.1
1,154,487 - -0.7
1,154,487 - +0.9
1,154,487 - -0.7
1,154,487 - +0.3
1,154,487 - -0.8
1,154,504 + -0.2
1,154,577 - Pf1N1B4_1019 0.15 -0.8
1,154,793 + Pf1N1B4_1019 0.37 +0.2
1,154,793 + Pf1N1B4_1019 0.37 -0.7
1,154,793 + Pf1N1B4_1019 0.37 -1.2
1,154,794 - Pf1N1B4_1019 0.37 -1.2
1,154,794 - Pf1N1B4_1019 0.37 +0.9
1,154,794 - Pf1N1B4_1019 0.37 +0.2
1,154,880 - Pf1N1B4_1019 0.46 +0.2
1,154,902 + Pf1N1B4_1019 0.48 -0.4
1,154,902 + Pf1N1B4_1019 0.48 +0.6
1,154,903 - Pf1N1B4_1019 0.48 +1.2
1,154,903 - Pf1N1B4_1019 0.48 +1.2
1,155,146 + Pf1N1B4_1019 0.73 -0.8
1,155,146 + Pf1N1B4_1019 0.73 +1.0
1,155,146 + Pf1N1B4_1019 0.73 -0.2
1,155,146 + Pf1N1B4_1019 0.73 +0.2
1,155,147 - Pf1N1B4_1019 0.73 -0.8
1,155,147 - Pf1N1B4_1019 0.73 +0.9
1,155,147 - +1.2
1,155,147 - Pf1N1B4_1019 0.73 +0.3
1,155,311 - Pf1N1B4_1019 0.89 -0.6
1,155,495 + +0.2
1,155,495 + -1.7
1,155,495 + +1.2
1,155,588 - Pf1N1B4_1020 0.18 +0.9
1,155,631 + Pf1N1B4_1020 0.22 +1.8
1,156,157 - Pf1N1B4_1020 0.75 +0.9
1,156,179 + Pf1N1B4_1020 0.77 -0.3
1,156,179 + Pf1N1B4_1020 0.77 +0.8
1,156,180 - Pf1N1B4_1020 0.77 -1.2
1,156,180 - Pf1N1B4_1020 0.77 +0.2
1,156,180 - Pf1N1B4_1020 0.77 -1.5
1,156,239 + Pf1N1B4_1020 0.83 +0.4
1,156,240 - Pf1N1B4_1020 0.83 -0.4
1,156,250 + Pf1N1B4_1020 0.84 -1.7
1,156,250 + Pf1N1B4_1020 0.84 +0.3
1,156,250 + Pf1N1B4_1020 0.84 +0.0
1,156,250 + Pf1N1B4_1020 0.84 -1.8
1,156,251 - Pf1N1B4_1020 0.84 -1.3
1,156,251 - +1.2
1,156,251 - Pf1N1B4_1020 0.84 -1.2
1,156,475 + +0.6
1,156,475 + -0.7
1,156,618 - -0.3
1,156,618 - -0.0
1,156,770 + Pf1N1B4_1021 0.16 -0.1
1,156,770 + Pf1N1B4_1021 0.16 +0.6
1,156,770 + Pf1N1B4_1021 0.16 +1.2
1,156,770 + Pf1N1B4_1021 0.16 -1.7
1,156,770 + Pf1N1B4_1021 0.16 -0.3
1,156,770 + Pf1N1B4_1021 0.16 -0.4
1,156,770 + Pf1N1B4_1021 0.16 -0.7
1,156,770 + Pf1N1B4_1021 0.16 +0.9
1,156,770 + Pf1N1B4_1021 0.16 -0.2
1,156,771 - Pf1N1B4_1021 0.16 -0.3
1,156,771 - Pf1N1B4_1021 0.16 -1.0
1,156,771 - Pf1N1B4_1021 0.16 -1.8
1,156,771 - Pf1N1B4_1021 0.16 -1.0
1,156,771 - Pf1N1B4_1021 0.16 +0.8
1,156,771 - Pf1N1B4_1021 0.16 +0.2
1,156,771 - Pf1N1B4_1021 0.16 +1.4
1,156,771 - Pf1N1B4_1021 0.16 -0.8
1,156,771 - Pf1N1B4_1021 0.16 +0.6
1,156,797 + Pf1N1B4_1021 0.18 -1.2
1,156,797 + Pf1N1B4_1021 0.18 -1.0
1,156,809 + Pf1N1B4_1021 0.20 -0.4
1,156,810 - Pf1N1B4_1021 0.20 +0.4
1,156,918 + Pf1N1B4_1021 0.30 +0.4
1,156,918 + Pf1N1B4_1021 0.30 +0.3
1,156,949 + Pf1N1B4_1021 0.33 -0.0
1,156,949 + Pf1N1B4_1021 0.33 -0.4
1,156,949 + Pf1N1B4_1021 0.33 -0.4
1,156,949 + Pf1N1B4_1021 0.33 -0.2
1,156,949 + Pf1N1B4_1021 0.33 +0.7
1,156,950 - Pf1N1B4_1021 0.33 +1.6
1,156,950 - Pf1N1B4_1021 0.33 -0.3
1,157,072 + Pf1N1B4_1021 0.45 +1.2
1,157,072 + Pf1N1B4_1021 0.45 -1.3
1,157,072 + Pf1N1B4_1021 0.45 -0.5
1,157,072 + Pf1N1B4_1021 0.45 -0.2
1,157,141 - Pf1N1B4_1021 0.52 -2.8
1,157,307 + Pf1N1B4_1021 0.68 -0.1
1,157,307 + Pf1N1B4_1021 0.68 +0.9
1,157,307 + Pf1N1B4_1021 0.68 +0.6
1,157,308 - Pf1N1B4_1021 0.68 -1.2
1,157,308 - Pf1N1B4_1021 0.68 +0.9
1,157,308 - Pf1N1B4_1021 0.68 -0.8
1,157,352 - Pf1N1B4_1021 0.73 +0.2
1,157,352 - Pf1N1B4_1021 0.73 -0.4

Or see this region's nucleotide sequence