Experiment: PYE with nitrite 2.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_02076 and CCNA_02077 overlap by 4 nucleotides CCNA_02077 and CCNA_02078 overlap by 4 nucleotides CCNA_02078 and CCNA_03896 are separated by 366 nucleotides
CCNA_02076: CCNA_02076 - protein-L-isoaspartate O-methyltransferase, at 2,226,974 to 2,227,642
_02076
CCNA_02077: CCNA_02077 - 5'(3')-nucleotidase/polyphosphatase SurE, at 2,227,639 to 2,228,430
_02077
CCNA_02078: CCNA_02078 - seryl-tRNA synthetase, at 2,228,427 to 2,229,875
_02078
CCNA_03896: CCNA_03896 - hypothetical protein, at 2,230,242 to 2,230,709
_03896
Position (kb)
2228
2229
2230 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2227.602 kb on + strand at 2227.610 kb on - strand at 2227.610 kb on - strand at 2227.687 kb on - strand at 2227.697 kb on + strand at 2227.845 kb on + strand, within CCNA_02077 at 2227.870 kb on + strand, within CCNA_02077 at 2227.955 kb on + strand, within CCNA_02077 at 2228.007 kb on - strand, within CCNA_02077 at 2228.007 kb on - strand, within CCNA_02077 at 2228.131 kb on - strand, within CCNA_02077 at 2228.221 kb on + strand, within CCNA_02077 at 2228.221 kb on + strand, within CCNA_02077 at 2228.285 kb on + strand, within CCNA_02077 at 2228.293 kb on - strand, within CCNA_02077 at 2228.374 kb on - strand at 2228.386 kb on - strand at 2230.116 kb on - strand at 2230.116 kb on - strand at 2230.116 kb on - strand at 2230.147 kb on - strand at 2230.184 kb on - strand at 2230.224 kb on + strand at 2230.232 kb on - strand at 2230.241 kb on - strand at 2230.280 kb on - strand at 2230.316 kb on + strand, within CCNA_03896 at 2230.318 kb on - strand, within CCNA_03896 at 2230.349 kb on + strand, within CCNA_03896 at 2230.351 kb on + strand, within CCNA_03896 at 2230.357 kb on - strand, within CCNA_03896 at 2230.382 kb on - strand, within CCNA_03896 at 2230.394 kb on + strand, within CCNA_03896 at 2230.415 kb on + strand, within CCNA_03896 at 2230.422 kb on + strand, within CCNA_03896 at 2230.422 kb on + strand, within CCNA_03896 at 2230.422 kb on + strand, within CCNA_03896 at 2230.422 kb on + strand, within CCNA_03896 at 2230.430 kb on - strand, within CCNA_03896 at 2230.430 kb on - strand, within CCNA_03896 at 2230.430 kb on - strand, within CCNA_03896 at 2230.430 kb on - strand, within CCNA_03896 at 2230.430 kb on - strand, within CCNA_03896 at 2230.430 kb on - strand, within CCNA_03896 at 2230.430 kb on - strand, within CCNA_03896 at 2230.430 kb on - strand, within CCNA_03896 at 2230.456 kb on - strand, within CCNA_03896 at 2230.511 kb on + strand, within CCNA_03896 at 2230.511 kb on + strand, within CCNA_03896 at 2230.511 kb on - strand, within CCNA_03896 at 2230.522 kb on + strand, within CCNA_03896 at 2230.547 kb on + strand, within CCNA_03896 at 2230.565 kb on + strand, within CCNA_03896 at 2230.573 kb on - strand, within CCNA_03896 at 2230.674 kb on + strand at 2230.676 kb on - strand at 2230.680 kb on + strand at 2230.688 kb on - strand at 2230.688 kb on - strand at 2230.699 kb on - strand at 2230.715 kb on + strand at 2230.796 kb on - strand at 2230.844 kb on + strand at 2230.849 kb on + strand at 2230.852 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with nitrite 2.5 mM remove 2,227,602 + -1.4 2,227,610 - +3.3 2,227,610 - -0.8 2,227,687 - +1.9 2,227,697 + +0.0 2,227,845 + CCNA_02077 0.26 -2.6 2,227,870 + CCNA_02077 0.29 -1.5 2,227,955 + CCNA_02077 0.40 -1.0 2,228,007 - CCNA_02077 0.46 +1.5 2,228,007 - CCNA_02077 0.46 +2.1 2,228,131 - CCNA_02077 0.62 +2.1 2,228,221 + CCNA_02077 0.73 +1.8 2,228,221 + CCNA_02077 0.73 -1.2 2,228,285 + CCNA_02077 0.82 -1.7 2,228,293 - CCNA_02077 0.83 -1.0 2,228,374 - +2.5 2,228,386 - +2.2 2,230,116 - +0.5 2,230,116 - -1.0 2,230,116 - -2.1 2,230,147 - -0.0 2,230,184 - -1.3 2,230,224 + +1.2 2,230,232 - +0.1 2,230,241 - -0.3 2,230,280 - -1.4 2,230,316 + CCNA_03896 0.16 +0.6 2,230,318 - CCNA_03896 0.16 +0.5 2,230,349 + CCNA_03896 0.23 +1.5 2,230,351 + CCNA_03896 0.23 +2.2 2,230,357 - CCNA_03896 0.25 -0.7 2,230,382 - CCNA_03896 0.30 -0.6 2,230,394 + CCNA_03896 0.32 +2.5 2,230,415 + CCNA_03896 0.37 +1.6 2,230,422 + CCNA_03896 0.38 +1.9 2,230,422 + CCNA_03896 0.38 +1.5 2,230,422 + CCNA_03896 0.38 +2.0 2,230,422 + CCNA_03896 0.38 +1.7 2,230,430 - CCNA_03896 0.40 -1.4 2,230,430 - CCNA_03896 0.40 -0.3 2,230,430 - CCNA_03896 0.40 -2.5 2,230,430 - CCNA_03896 0.40 -2.2 2,230,430 - CCNA_03896 0.40 -3.3 2,230,430 - CCNA_03896 0.40 -1.0 2,230,430 - CCNA_03896 0.40 -2.3 2,230,430 - CCNA_03896 0.40 -2.1 2,230,456 - CCNA_03896 0.46 -0.3 2,230,511 + CCNA_03896 0.57 +1.6 2,230,511 + CCNA_03896 0.57 +1.1 2,230,511 - CCNA_03896 0.57 -0.7 2,230,522 + CCNA_03896 0.60 +1.6 2,230,547 + CCNA_03896 0.65 +1.6 2,230,565 + CCNA_03896 0.69 +2.2 2,230,573 - CCNA_03896 0.71 -0.7 2,230,674 + +0.2 2,230,676 - -0.1 2,230,680 + +2.2 2,230,688 - -0.8 2,230,688 - -1.4 2,230,699 - -1.8 2,230,715 + +2.0 2,230,796 - +0.6 2,230,844 + -2.2 2,230,849 + -1.0 2,230,852 - +1.4
Or see this region's nucleotide sequence