Experiment: PYE with nitrite 2.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_00408 and CCNA_00409 are separated by 97 nucleotides CCNA_00409 and CCNA_00410 are separated by 62 nucleotides CCNA_00410 and CCNA_00411 are separated by 32 nucleotides CCNA_00411 and CCNA_00412 overlap by 4 nucleotides CCNA_00412 and CCNA_00413 are separated by 92 nucleotides
CCNA_00408: CCNA_00408 - antiporter protein, at 423,040 to 424,308
_00408
CCNA_00409: CCNA_00409 - hypothetical protein, at 424,406 to 424,450
_00409
CCNA_00410: CCNA_00410 - acetyltransferase, at 424,513 to 425,043
_00410
CCNA_00411: CCNA_00411 - hypothetical protein, at 425,076 to 425,639
_00411
CCNA_00412: CCNA_00412 - CoA pyrophosphatase, at 425,636 to 426,286
_00412
CCNA_00413: CCNA_00413 - poly(A) polymerase/tRNA-nucleotidyltransferase, at 426,379 to 427,632
_00413
Position (kb) 425
426 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 424.076 kb on + strand, within CCNA_00408 at 424.092 kb on + strand, within CCNA_00408 at 424.159 kb on - strand, within CCNA_00408 at 424.159 kb on - strand, within CCNA_00408 at 424.159 kb on - strand, within CCNA_00408 at 424.168 kb on - strand, within CCNA_00408 at 424.199 kb on - strand at 424.205 kb on - strand at 424.205 kb on - strand at 424.205 kb on - strand at 424.217 kb on + strand at 424.217 kb on + strand at 424.217 kb on + strand at 424.217 kb on + strand at 424.217 kb on + strand at 424.217 kb on + strand at 424.217 kb on + strand at 424.222 kb on + strand at 424.225 kb on - strand at 424.225 kb on - strand at 424.225 kb on - strand at 424.225 kb on - strand at 424.230 kb on - strand at 424.230 kb on - strand at 424.264 kb on + strand at 424.342 kb on - strand at 424.346 kb on + strand at 424.363 kb on + strand at 424.365 kb on - strand at 424.368 kb on + strand at 424.369 kb on + strand at 424.369 kb on + strand at 424.369 kb on + strand at 424.369 kb on + strand at 424.369 kb on + strand at 424.369 kb on + strand at 424.371 kb on - strand at 424.377 kb on - strand at 424.377 kb on - strand at 424.377 kb on - strand at 424.404 kb on - strand at 424.445 kb on + strand, within CCNA_00409 at 424.452 kb on + strand at 424.465 kb on + strand at 424.473 kb on - strand at 424.473 kb on - strand at 424.481 kb on + strand at 424.489 kb on + strand at 424.530 kb on - strand at 424.728 kb on + strand, within CCNA_00410 at 424.795 kb on - strand, within CCNA_00410 at 424.999 kb on + strand at 425.010 kb on + strand at 425.024 kb on + strand at 425.032 kb on - strand at 425.066 kb on + strand at 425.073 kb on + strand at 425.081 kb on - strand at 425.088 kb on + strand at 425.088 kb on + strand at 425.088 kb on + strand at 425.088 kb on + strand at 425.088 kb on + strand at 425.088 kb on + strand at 425.090 kb on + strand at 425.090 kb on + strand at 425.090 kb on + strand at 425.090 kb on + strand at 425.096 kb on - strand at 425.096 kb on - strand at 425.100 kb on + strand at 425.100 kb on + strand at 425.100 kb on + strand at 425.103 kb on - strand at 425.110 kb on + strand at 425.120 kb on + strand at 425.140 kb on + strand, within CCNA_00411 at 425.165 kb on - strand, within CCNA_00411 at 425.221 kb on + strand, within CCNA_00411 at 425.248 kb on - strand, within CCNA_00411 at 425.299 kb on + strand, within CCNA_00411 at 425.299 kb on + strand, within CCNA_00411 at 425.307 kb on - strand, within CCNA_00411 at 425.314 kb on - strand, within CCNA_00411 at 425.335 kb on - strand, within CCNA_00411 at 425.354 kb on + strand, within CCNA_00411 at 425.362 kb on - strand, within CCNA_00411 at 425.362 kb on - strand, within CCNA_00411 at 425.362 kb on - strand, within CCNA_00411 at 425.367 kb on + strand, within CCNA_00411 at 425.377 kb on + strand, within CCNA_00411 at 425.465 kb on + strand, within CCNA_00411 at 425.505 kb on + strand, within CCNA_00411 at 425.536 kb on + strand, within CCNA_00411 at 425.536 kb on + strand, within CCNA_00411 at 425.559 kb on + strand, within CCNA_00411 at 425.628 kb on + strand at 425.628 kb on + strand at 425.628 kb on + strand at 425.629 kb on - strand at 425.633 kb on + strand at 425.636 kb on - strand at 425.719 kb on + strand, within CCNA_00412 at 425.800 kb on + strand, within CCNA_00412 at 425.800 kb on + strand, within CCNA_00412 at 425.811 kb on + strand, within CCNA_00412 at 425.836 kb on - strand, within CCNA_00412 at 425.849 kb on + strand, within CCNA_00412 at 425.865 kb on - strand, within CCNA_00412 at 425.900 kb on - strand, within CCNA_00412 at 425.920 kb on - strand, within CCNA_00412 at 425.920 kb on - strand, within CCNA_00412 at 425.924 kb on + strand, within CCNA_00412 at 425.924 kb on + strand, within CCNA_00412 at 425.932 kb on - strand, within CCNA_00412 at 425.960 kb on - strand, within CCNA_00412 at 425.968 kb on + strand, within CCNA_00412 at 425.987 kb on + strand, within CCNA_00412 at 426.012 kb on + strand, within CCNA_00412 at 426.057 kb on + strand, within CCNA_00412 at 426.064 kb on + strand, within CCNA_00412 at 426.069 kb on + strand, within CCNA_00412 at 426.069 kb on + strand, within CCNA_00412 at 426.069 kb on + strand, within CCNA_00412 at 426.069 kb on + strand, within CCNA_00412 at 426.069 kb on + strand, within CCNA_00412 at 426.069 kb on + strand, within CCNA_00412 at 426.077 kb on - strand, within CCNA_00412 at 426.077 kb on - strand, within CCNA_00412 at 426.077 kb on - strand, within CCNA_00412 at 426.077 kb on - strand, within CCNA_00412 at 426.077 kb on - strand, within CCNA_00412 at 426.077 kb on - strand, within CCNA_00412 at 426.077 kb on - strand, within CCNA_00412 at 426.119 kb on + strand, within CCNA_00412 at 426.137 kb on + strand, within CCNA_00412 at 426.195 kb on + strand, within CCNA_00412 at 426.213 kb on - strand, within CCNA_00412 at 426.241 kb on + strand at 426.241 kb on + strand at 426.271 kb on + strand at 426.276 kb on + strand at 426.276 kb on + strand at 426.276 kb on + strand at 426.276 kb on + strand at 426.284 kb on - strand at 426.284 kb on - strand at 426.318 kb on - strand at 426.321 kb on - strand at 426.329 kb on + strand at 426.344 kb on - strand at 426.345 kb on - strand at 426.376 kb on + strand at 426.384 kb on - strand at 426.391 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with nitrite 2.5 mM remove 424,076 + CCNA_00408 0.82 -1.1 424,092 + CCNA_00408 0.83 -0.7 424,159 - CCNA_00408 0.88 -1.0 424,159 - CCNA_00408 0.88 +1.2 424,159 - CCNA_00408 0.88 +1.9 424,168 - CCNA_00408 0.89 -0.2 424,199 - -0.8 424,205 - -0.4 424,205 - +1.4 424,205 - +1.2 424,217 + -1.9 424,217 + -0.7 424,217 + -0.3 424,217 + +0.1 424,217 + -1.2 424,217 + -0.1 424,217 + +1.0 424,222 + -0.9 424,225 - +0.1 424,225 - +0.1 424,225 - -1.6 424,225 - +1.1 424,230 - +1.1 424,230 - +0.0 424,264 + -1.3 424,342 - -1.9 424,346 + -1.1 424,363 + -0.3 424,365 - -1.3 424,368 + +1.9 424,369 + +1.9 424,369 + +1.9 424,369 + +2.3 424,369 + +1.7 424,369 + +2.1 424,369 + +2.1 424,371 - -0.9 424,377 - -1.4 424,377 - -1.8 424,377 - +1.1 424,404 - +0.8 424,445 + CCNA_00409 0.87 +0.8 424,452 + +0.0 424,465 + +1.1 424,473 - -0.3 424,473 - -2.6 424,481 + +1.2 424,489 + +0.9 424,530 - +0.1 424,728 + CCNA_00410 0.40 +1.2 424,795 - CCNA_00410 0.53 +1.7 424,999 + -0.2 425,010 + -0.1 425,024 + +1.6 425,032 - +0.5 425,066 + +0.4 425,073 + +0.6 425,081 - -0.0 425,088 + +0.1 425,088 + +0.9 425,088 + +0.7 425,088 + +2.3 425,088 + +0.4 425,088 + -1.7 425,090 + -1.8 425,090 + +1.0 425,090 + +0.2 425,090 + -0.5 425,096 - +0.2 425,096 - -0.8 425,100 + +0.5 425,100 + +0.5 425,100 + +0.5 425,103 - -1.4 425,110 + +1.4 425,120 + +0.1 425,140 + CCNA_00411 0.11 +0.7 425,165 - CCNA_00411 0.16 -1.5 425,221 + CCNA_00411 0.26 +0.7 425,248 - CCNA_00411 0.30 -1.3 425,299 + CCNA_00411 0.40 +1.3 425,299 + CCNA_00411 0.40 +0.5 425,307 - CCNA_00411 0.41 -0.1 425,314 - CCNA_00411 0.42 -0.1 425,335 - CCNA_00411 0.46 +0.5 425,354 + CCNA_00411 0.49 +0.6 425,362 - CCNA_00411 0.51 -0.9 425,362 - CCNA_00411 0.51 -0.1 425,362 - CCNA_00411 0.51 -0.9 425,367 + CCNA_00411 0.52 -0.4 425,377 + CCNA_00411 0.53 +0.1 425,465 + CCNA_00411 0.69 +0.7 425,505 + CCNA_00411 0.76 +0.3 425,536 + CCNA_00411 0.82 +0.2 425,536 + CCNA_00411 0.82 +0.6 425,559 + CCNA_00411 0.86 +0.6 425,628 + +0.3 425,628 + +0.8 425,628 + +0.1 425,629 - -1.5 425,633 + +0.8 425,636 - +0.8 425,719 + CCNA_00412 0.13 +0.3 425,800 + CCNA_00412 0.25 -0.5 425,800 + CCNA_00412 0.25 +0.6 425,811 + CCNA_00412 0.27 +0.9 425,836 - CCNA_00412 0.31 -0.2 425,849 + CCNA_00412 0.33 +0.7 425,865 - CCNA_00412 0.35 +0.8 425,900 - CCNA_00412 0.41 +0.2 425,920 - CCNA_00412 0.44 +0.5 425,920 - CCNA_00412 0.44 +0.7 425,924 + CCNA_00412 0.44 +0.3 425,924 + CCNA_00412 0.44 -0.8 425,932 - CCNA_00412 0.45 -1.0 425,960 - CCNA_00412 0.50 -0.2 425,968 + CCNA_00412 0.51 +0.5 425,987 + CCNA_00412 0.54 +0.2 426,012 + CCNA_00412 0.58 +0.1 426,057 + CCNA_00412 0.65 +1.2 426,064 + CCNA_00412 0.66 +1.7 426,069 + CCNA_00412 0.67 -1.9 426,069 + CCNA_00412 0.67 +1.0 426,069 + CCNA_00412 0.67 -1.4 426,069 + CCNA_00412 0.67 +0.3 426,069 + CCNA_00412 0.67 -0.2 426,069 + CCNA_00412 0.67 +1.0 426,077 - CCNA_00412 0.68 -2.1 426,077 - CCNA_00412 0.68 -0.2 426,077 - CCNA_00412 0.68 -0.5 426,077 - CCNA_00412 0.68 -0.0 426,077 - CCNA_00412 0.68 -0.5 426,077 - CCNA_00412 0.68 -0.5 426,077 - CCNA_00412 0.68 +1.1 426,119 + CCNA_00412 0.74 -0.0 426,137 + CCNA_00412 0.77 -0.1 426,195 + CCNA_00412 0.86 -0.6 426,213 - CCNA_00412 0.89 -1.4 426,241 + +0.5 426,241 + +0.3 426,271 + -0.5 426,276 + +1.0 426,276 + -0.0 426,276 + +0.2 426,276 + +0.7 426,284 - -1.1 426,284 - -0.1 426,318 - -2.4 426,321 - -1.3 426,329 + +1.3 426,344 - -2.5 426,345 - -0.5 426,376 + +3.8 426,384 - -1.6 426,391 - +0.3
Or see this region's nucleotide sequence