Experiment: Tetracycline 0.024 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR86_RS08780 and ABZR86_RS08785 are separated by 57 nucleotides ABZR86_RS08785 and ABZR86_RS08790 overlap by 8 nucleotides ABZR86_RS08790 and ABZR86_RS08795 are separated by 93 nucleotides ABZR86_RS08795 and ABZR86_RS08800 are separated by 84 nucleotides
ABZR86_RS08780: ABZR86_RS08780 - bifunctional 2-polyprenyl-6-hydroxyphenol methylase/3-demethylubiquinol 3-O-methyltransferase UbiG, at 1,964,445 to 1,965,152
_RS08780
ABZR86_RS08785: ABZR86_RS08785 - YciI family protein, at 1,965,210 to 1,965,482
_RS08785
ABZR86_RS08790: ABZR86_RS08790 - TRZ/ATZ family hydrolase, at 1,965,475 to 1,966,815
_RS08790
ABZR86_RS08795: ABZR86_RS08795 - elongation factor P, at 1,966,909 to 1,967,478
_RS08795
ABZR86_RS08800: ABZR86_RS08800 - EF-P beta-lysylation protein EpmB, at 1,967,563 to 1,968,573
_RS08800
Position (kb) 1965
1966
1967 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 1965.160 kb on - strand at 1965.175 kb on - strand at 1965.175 kb on - strand at 1965.175 kb on - strand at 1965.175 kb on - strand at 1965.175 kb on - strand at 1965.175 kb on - strand at 1965.175 kb on - strand at 1965.206 kb on + strand at 1965.206 kb on + strand at 1965.207 kb on - strand at 1965.207 kb on - strand at 1965.207 kb on - strand at 1965.207 kb on - strand at 1965.207 kb on - strand at 1965.257 kb on + strand, within ABZR86_RS08785 at 1965.257 kb on + strand, within ABZR86_RS08785 at 1965.257 kb on + strand, within ABZR86_RS08785 at 1965.258 kb on - strand, within ABZR86_RS08785 at 1965.258 kb on - strand, within ABZR86_RS08785 at 1965.258 kb on - strand, within ABZR86_RS08785 at 1965.259 kb on + strand, within ABZR86_RS08785 at 1965.259 kb on + strand, within ABZR86_RS08785 at 1965.259 kb on + strand, within ABZR86_RS08785 at 1965.259 kb on + strand, within ABZR86_RS08785 at 1965.259 kb on + strand, within ABZR86_RS08785 at 1965.259 kb on + strand, within ABZR86_RS08785 at 1965.260 kb on - strand, within ABZR86_RS08785 at 1965.260 kb on - strand, within ABZR86_RS08785 at 1965.260 kb on - strand, within ABZR86_RS08785 at 1965.260 kb on - strand, within ABZR86_RS08785 at 1965.281 kb on + strand, within ABZR86_RS08785 at 1965.282 kb on - strand, within ABZR86_RS08785 at 1965.282 kb on - strand, within ABZR86_RS08785 at 1965.319 kb on + strand, within ABZR86_RS08785 at 1965.320 kb on - strand, within ABZR86_RS08785 at 1965.320 kb on - strand, within ABZR86_RS08785 at 1965.454 kb on + strand, within ABZR86_RS08785 at 1965.455 kb on - strand at 1965.455 kb on - strand at 1965.626 kb on + strand, within ABZR86_RS08790 at 1965.627 kb on - strand, within ABZR86_RS08790 at 1965.627 kb on - strand, within ABZR86_RS08790 at 1965.627 kb on - strand, within ABZR86_RS08790 at 1965.627 kb on - strand, within ABZR86_RS08790 at 1965.627 kb on - strand, within ABZR86_RS08790 at 1965.627 kb on - strand, within ABZR86_RS08790 at 1965.782 kb on + strand, within ABZR86_RS08790 at 1965.783 kb on - strand, within ABZR86_RS08790 at 1966.223 kb on + strand, within ABZR86_RS08790 at 1966.223 kb on + strand, within ABZR86_RS08790 at 1966.223 kb on + strand, within ABZR86_RS08790 at 1966.223 kb on + strand, within ABZR86_RS08790 at 1966.223 kb on + strand, within ABZR86_RS08790 at 1966.223 kb on + strand, within ABZR86_RS08790 at 1966.223 kb on + strand, within ABZR86_RS08790 at 1966.223 kb on + strand, within ABZR86_RS08790 at 1966.223 kb on + strand, within ABZR86_RS08790 at 1966.224 kb on - strand, within ABZR86_RS08790 at 1966.224 kb on - strand, within ABZR86_RS08790 at 1966.224 kb on - strand, within ABZR86_RS08790 at 1966.224 kb on - strand, within ABZR86_RS08790 at 1966.224 kb on - strand, within ABZR86_RS08790 at 1966.304 kb on + strand, within ABZR86_RS08790 at 1966.304 kb on + strand, within ABZR86_RS08790 at 1966.305 kb on - strand, within ABZR86_RS08790 at 1966.385 kb on + strand, within ABZR86_RS08790 at 1966.385 kb on + strand, within ABZR86_RS08790 at 1966.385 kb on + strand, within ABZR86_RS08790 at 1966.386 kb on - strand, within ABZR86_RS08790 at 1966.386 kb on - strand, within ABZR86_RS08790 at 1966.415 kb on + strand, within ABZR86_RS08790 at 1966.416 kb on - strand, within ABZR86_RS08790 at 1966.416 kb on - strand, within ABZR86_RS08790 at 1966.596 kb on + strand, within ABZR86_RS08790 at 1966.596 kb on + strand, within ABZR86_RS08790 at 1966.597 kb on - strand, within ABZR86_RS08790 at 1966.597 kb on - strand, within ABZR86_RS08790 at 1966.597 kb on - strand, within ABZR86_RS08790 at 1966.597 kb on - strand, within ABZR86_RS08790 at 1966.661 kb on + strand, within ABZR86_RS08790 at 1966.662 kb on - strand, within ABZR86_RS08790 at 1966.820 kb on + strand at 1966.821 kb on - strand at 1966.850 kb on + strand at 1966.850 kb on + strand at 1966.851 kb on - strand at 1966.851 kb on - strand at 1966.852 kb on + strand at 1966.853 kb on - strand at 1966.853 kb on - strand at 1966.910 kb on + strand at 1966.914 kb on + strand at 1966.915 kb on - strand at 1967.518 kb on - strand at 1967.518 kb on - strand at 1967.522 kb on + strand at 1967.523 kb on - strand at 1967.591 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Tetracycline 0.024 mg/ml remove 1,965,160 - -0.4 1,965,175 - -0.1 1,965,175 - +0.3 1,965,175 - -0.2 1,965,175 - -0.5 1,965,175 - -3.6 1,965,175 - -1.0 1,965,175 - +0.8 1,965,206 + +0.3 1,965,206 + -1.0 1,965,207 - +0.4 1,965,207 - -0.4 1,965,207 - -1.0 1,965,207 - -0.4 1,965,207 - +0.3 1,965,257 + ABZR86_RS08785 0.17 -3.5 1,965,257 + ABZR86_RS08785 0.17 -0.4 1,965,257 + ABZR86_RS08785 0.17 -0.1 1,965,258 - ABZR86_RS08785 0.18 +0.4 1,965,258 - ABZR86_RS08785 0.18 +0.6 1,965,258 - ABZR86_RS08785 0.18 +1.0 1,965,259 + ABZR86_RS08785 0.18 +0.2 1,965,259 + ABZR86_RS08785 0.18 -0.7 1,965,259 + ABZR86_RS08785 0.18 +0.0 1,965,259 + ABZR86_RS08785 0.18 -0.2 1,965,259 + ABZR86_RS08785 0.18 +0.3 1,965,259 + ABZR86_RS08785 0.18 +0.4 1,965,260 - ABZR86_RS08785 0.18 -0.3 1,965,260 - ABZR86_RS08785 0.18 +0.3 1,965,260 - ABZR86_RS08785 0.18 +0.0 1,965,260 - ABZR86_RS08785 0.18 +0.7 1,965,281 + ABZR86_RS08785 0.26 -0.2 1,965,282 - ABZR86_RS08785 0.26 -0.6 1,965,282 - ABZR86_RS08785 0.26 +0.9 1,965,319 + ABZR86_RS08785 0.40 +0.0 1,965,320 - ABZR86_RS08785 0.40 -0.2 1,965,320 - ABZR86_RS08785 0.40 +0.9 1,965,454 + ABZR86_RS08785 0.89 -0.1 1,965,455 - +0.6 1,965,455 - +1.4 1,965,626 + ABZR86_RS08790 0.11 +1.4 1,965,627 - ABZR86_RS08790 0.11 -0.2 1,965,627 - ABZR86_RS08790 0.11 -0.8 1,965,627 - ABZR86_RS08790 0.11 +0.1 1,965,627 - ABZR86_RS08790 0.11 +0.0 1,965,627 - ABZR86_RS08790 0.11 +0.4 1,965,627 - ABZR86_RS08790 0.11 +0.4 1,965,782 + ABZR86_RS08790 0.23 +1.0 1,965,783 - ABZR86_RS08790 0.23 +0.7 1,966,223 + ABZR86_RS08790 0.56 -0.3 1,966,223 + ABZR86_RS08790 0.56 -0.6 1,966,223 + ABZR86_RS08790 0.56 -0.5 1,966,223 + ABZR86_RS08790 0.56 -1.5 1,966,223 + ABZR86_RS08790 0.56 -0.3 1,966,223 + ABZR86_RS08790 0.56 +1.7 1,966,223 + ABZR86_RS08790 0.56 -0.2 1,966,223 + ABZR86_RS08790 0.56 +0.6 1,966,223 + ABZR86_RS08790 0.56 +0.2 1,966,224 - ABZR86_RS08790 0.56 +1.8 1,966,224 - ABZR86_RS08790 0.56 -0.2 1,966,224 - ABZR86_RS08790 0.56 -0.1 1,966,224 - ABZR86_RS08790 0.56 -1.6 1,966,224 - ABZR86_RS08790 0.56 -0.5 1,966,304 + ABZR86_RS08790 0.62 -2.0 1,966,304 + ABZR86_RS08790 0.62 +0.7 1,966,305 - ABZR86_RS08790 0.62 +0.5 1,966,385 + ABZR86_RS08790 0.68 -1.5 1,966,385 + ABZR86_RS08790 0.68 -0.5 1,966,385 + ABZR86_RS08790 0.68 -1.7 1,966,386 - ABZR86_RS08790 0.68 -0.7 1,966,386 - ABZR86_RS08790 0.68 -0.1 1,966,415 + ABZR86_RS08790 0.70 -0.4 1,966,416 - ABZR86_RS08790 0.70 -0.9 1,966,416 - ABZR86_RS08790 0.70 -0.1 1,966,596 + ABZR86_RS08790 0.84 -0.6 1,966,596 + ABZR86_RS08790 0.84 -0.2 1,966,597 - ABZR86_RS08790 0.84 -2.2 1,966,597 - ABZR86_RS08790 0.84 -1.9 1,966,597 - ABZR86_RS08790 0.84 -1.0 1,966,597 - ABZR86_RS08790 0.84 +2.9 1,966,661 + ABZR86_RS08790 0.88 +0.6 1,966,662 - ABZR86_RS08790 0.89 +0.5 1,966,820 + +0.0 1,966,821 - +0.6 1,966,850 + +0.3 1,966,850 + -1.1 1,966,851 - -0.6 1,966,851 - -0.4 1,966,852 + +0.9 1,966,853 - +0.2 1,966,853 - +0.1 1,966,910 + +0.4 1,966,914 + -2.5 1,966,915 - +0.9 1,967,518 - -0.8 1,967,518 - +0.1 1,967,522 + -2.1 1,967,523 - -1.9 1,967,591 + +0.5
Or see this region's nucleotide sequence