Experiment: Cholin acetate 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_826 and Pf1N1B4_827 are separated by 129 nucleotides Pf1N1B4_827 and Pf1N1B4_828 overlap by 1 nucleotides Pf1N1B4_828 and Pf1N1B4_829 are separated by 475 nucleotides
Pf1N1B4_826 - tRNA (5-methoxyuridine) 34 synthase, at 947,409 to 948,365
_826
Pf1N1B4_827 - tRNA-specific adenosine-34 deaminase (EC 3.5.4.-), at 948,495 to 948,992
_827
Pf1N1B4_828 - Multicopper oxidase, at 948,992 to 950,368
_828
Pf1N1B4_829 - Mobile element protein, at 950,844 to 951,680
_829
Position (kb) 948
949
950
951 Strain fitness (log2 ratio) -2
-1
0
1
2 at 947.995 kb on - strand, within Pf1N1B4_826 at 948.267 kb on + strand at 948.267 kb on + strand, within Pf1N1B4_826 at 948.267 kb on + strand, within Pf1N1B4_826 at 948.267 kb on + strand, within Pf1N1B4_826 at 948.267 kb on + strand, within Pf1N1B4_826 at 948.268 kb on - strand, within Pf1N1B4_826 at 948.363 kb on + strand at 948.363 kb on + strand at 948.363 kb on + strand at 948.388 kb on + strand at 948.388 kb on + strand at 948.389 kb on - strand at 948.497 kb on - strand at 948.497 kb on - strand at 949.120 kb on - strand at 949.120 kb on - strand at 949.120 kb on - strand at 949.120 kb on - strand at 949.135 kb on - strand, within Pf1N1B4_828 at 949.135 kb on - strand, within Pf1N1B4_828 at 949.237 kb on - strand, within Pf1N1B4_828 at 949.237 kb on - strand, within Pf1N1B4_828 at 949.561 kb on + strand, within Pf1N1B4_828 at 949.561 kb on + strand, within Pf1N1B4_828 at 949.561 kb on + strand, within Pf1N1B4_828 at 949.561 kb on + strand, within Pf1N1B4_828 at 949.562 kb on - strand at 949.562 kb on - strand, within Pf1N1B4_828 at 949.632 kb on + strand at 949.632 kb on + strand, within Pf1N1B4_828 at 949.632 kb on + strand, within Pf1N1B4_828 at 949.632 kb on + strand, within Pf1N1B4_828 at 949.632 kb on + strand, within Pf1N1B4_828 at 949.633 kb on - strand, within Pf1N1B4_828 at 949.633 kb on - strand, within Pf1N1B4_828 at 949.633 kb on - strand, within Pf1N1B4_828 at 949.633 kb on - strand, within Pf1N1B4_828 at 949.668 kb on + strand, within Pf1N1B4_828 at 949.965 kb on + strand, within Pf1N1B4_828 at 949.966 kb on - strand, within Pf1N1B4_828 at 949.966 kb on - strand, within Pf1N1B4_828 at 949.993 kb on + strand, within Pf1N1B4_828 at 949.993 kb on + strand, within Pf1N1B4_828 at 949.994 kb on - strand at 949.995 kb on + strand, within Pf1N1B4_828 at 949.995 kb on + strand, within Pf1N1B4_828 at 949.996 kb on - strand, within Pf1N1B4_828 at 950.037 kb on + strand, within Pf1N1B4_828 at 950.037 kb on + strand, within Pf1N1B4_828 at 950.038 kb on - strand, within Pf1N1B4_828 at 950.038 kb on - strand, within Pf1N1B4_828 at 950.244 kb on + strand at 950.245 kb on - strand at 950.245 kb on - strand at 950.245 kb on - strand at 950.245 kb on - strand at 950.345 kb on + strand at 950.346 kb on - strand at 950.346 kb on - strand at 950.346 kb on - strand at 950.378 kb on + strand at 950.378 kb on + strand at 950.378 kb on + strand at 950.378 kb on + strand at 950.378 kb on + strand at 950.378 kb on + strand at 950.378 kb on + strand at 950.378 kb on + strand at 950.378 kb on + strand at 950.378 kb on + strand at 950.379 kb on - strand at 950.379 kb on - strand at 950.379 kb on - strand at 950.379 kb on - strand at 950.379 kb on - strand at 950.379 kb on - strand at 950.379 kb on - strand at 950.387 kb on + strand at 950.387 kb on + strand at 950.387 kb on + strand at 950.387 kb on + strand at 950.388 kb on - strand at 950.388 kb on - strand at 950.490 kb on + strand at 950.491 kb on - strand at 950.517 kb on + strand at 950.517 kb on + strand at 950.517 kb on + strand at 950.517 kb on + strand at 950.517 kb on + strand at 950.518 kb on - strand at 950.518 kb on - strand at 950.518 kb on - strand at 950.533 kb on - strand at 950.542 kb on - strand at 950.542 kb on - strand at 950.542 kb on - strand at 950.612 kb on - strand at 950.636 kb on + strand at 950.636 kb on + strand at 950.664 kb on - strand at 950.664 kb on - strand at 950.743 kb on - strand at 950.753 kb on - strand at 950.768 kb on - strand at 950.775 kb on - strand at 950.775 kb on - strand at 950.820 kb on + strand at 950.820 kb on + strand at 950.821 kb on - strand at 950.821 kb on - strand at 950.821 kb on - strand at 950.838 kb on - strand at 950.865 kb on - strand at 950.890 kb on + strand at 950.890 kb on + strand at 950.890 kb on + strand at 950.891 kb on - strand at 950.891 kb on - strand at 950.891 kb on - strand at 950.891 kb on - strand at 950.891 kb on - strand at 950.894 kb on + strand at 950.894 kb on + strand at 950.894 kb on + strand at 950.894 kb on + strand at 950.895 kb on - strand at 950.895 kb on - strand at 950.895 kb on - strand at 950.895 kb on - strand at 950.895 kb on - strand at 950.915 kb on - strand at 950.927 kb on - strand at 950.927 kb on - strand at 950.927 kb on - strand at 950.927 kb on - strand at 951.106 kb on + strand, within Pf1N1B4_829 at 951.107 kb on - strand, within Pf1N1B4_829 at 951.265 kb on + strand, within Pf1N1B4_829 at 951.265 kb on + strand, within Pf1N1B4_829 at 951.266 kb on - strand, within Pf1N1B4_829 at 951.266 kb on - strand, within Pf1N1B4_829 at 951.266 kb on - strand, within Pf1N1B4_829 at 951.292 kb on + strand, within Pf1N1B4_829 at 951.292 kb on + strand, within Pf1N1B4_829 at 951.292 kb on + strand, within Pf1N1B4_829 at 951.292 kb on + strand at 951.293 kb on - strand, within Pf1N1B4_829 at 951.293 kb on - strand, within Pf1N1B4_829 at 951.293 kb on - strand, within Pf1N1B4_829 at 951.293 kb on - strand, within Pf1N1B4_829 at 951.293 kb on - strand, within Pf1N1B4_829 at 951.293 kb on - strand, within Pf1N1B4_829 at 951.293 kb on - strand, within Pf1N1B4_829
Per-strain Table
Position Strand Gene LocusTag Fraction Cholin acetate 20 mM remove 947,995 - Pf1N1B4_826 0.61 +0.1 948,267 + +2.0 948,267 + Pf1N1B4_826 0.90 -0.0 948,267 + Pf1N1B4_826 0.90 -0.6 948,267 + Pf1N1B4_826 0.90 -0.8 948,267 + Pf1N1B4_826 0.90 -1.8 948,268 - Pf1N1B4_826 0.90 +0.4 948,363 + -0.2 948,363 + +1.3 948,363 + -0.0 948,388 + +0.2 948,388 + +1.0 948,389 - +1.0 948,497 - -1.0 948,497 - +0.6 949,120 - +0.8 949,120 - +1.6 949,120 - -0.6 949,120 - -1.1 949,135 - Pf1N1B4_828 0.10 +1.0 949,135 - Pf1N1B4_828 0.10 +1.6 949,237 - Pf1N1B4_828 0.18 +0.4 949,237 - Pf1N1B4_828 0.18 -1.8 949,561 + Pf1N1B4_828 0.41 -1.1 949,561 + Pf1N1B4_828 0.41 -0.0 949,561 + Pf1N1B4_828 0.41 +0.6 949,561 + Pf1N1B4_828 0.41 -1.0 949,562 - +0.4 949,562 - Pf1N1B4_828 0.41 +1.4 949,632 + -0.0 949,632 + Pf1N1B4_828 0.46 -0.8 949,632 + Pf1N1B4_828 0.46 +0.5 949,632 + Pf1N1B4_828 0.46 -0.4 949,632 + Pf1N1B4_828 0.46 -0.8 949,633 - Pf1N1B4_828 0.47 -0.0 949,633 - Pf1N1B4_828 0.47 -2.4 949,633 - Pf1N1B4_828 0.47 -0.9 949,633 - Pf1N1B4_828 0.47 -0.6 949,668 + Pf1N1B4_828 0.49 -2.2 949,965 + Pf1N1B4_828 0.71 -1.8 949,966 - Pf1N1B4_828 0.71 -0.3 949,966 - Pf1N1B4_828 0.71 -0.8 949,993 + Pf1N1B4_828 0.73 -1.3 949,993 + Pf1N1B4_828 0.73 -0.0 949,994 - -0.6 949,995 + Pf1N1B4_828 0.73 -1.0 949,995 + Pf1N1B4_828 0.73 +1.0 949,996 - Pf1N1B4_828 0.73 -1.8 950,037 + Pf1N1B4_828 0.76 -0.6 950,037 + Pf1N1B4_828 0.76 +1.9 950,038 - Pf1N1B4_828 0.76 +0.7 950,038 - Pf1N1B4_828 0.76 +1.0 950,244 + +0.2 950,245 - +0.2 950,245 - +1.0 950,245 - -0.2 950,245 - -0.6 950,345 + -0.0 950,346 - +0.2 950,346 - -0.7 950,346 - +0.8 950,378 + +2.4 950,378 + +0.4 950,378 + +1.0 950,378 + -0.6 950,378 + -2.0 950,378 + -2.2 950,378 + -0.3 950,378 + +1.0 950,378 + -1.3 950,378 + -1.6 950,379 - +0.2 950,379 - +0.7 950,379 - +1.0 950,379 - -0.1 950,379 - +0.4 950,379 - -2.2 950,379 - +0.1 950,387 + +0.1 950,387 + -1.2 950,387 + -0.0 950,387 + +1.7 950,388 - -0.8 950,388 - -0.3 950,490 + +0.2 950,491 - +0.6 950,517 + +1.0 950,517 + -0.6 950,517 + -0.6 950,517 + -1.6 950,517 + -0.0 950,518 - -1.6 950,518 - -2.0 950,518 - +1.0 950,533 - +1.4 950,542 - +0.2 950,542 - +0.2 950,542 - -0.0 950,612 - -0.3 950,636 + +0.4 950,636 + -1.6 950,664 - +1.0 950,664 - -0.8 950,743 - -0.5 950,753 - +0.7 950,768 - +0.2 950,775 - +1.6 950,775 - -0.8 950,820 + +1.0 950,820 + -0.0 950,821 - +0.2 950,821 - -1.0 950,821 - +0.7 950,838 - -0.0 950,865 - -0.1 950,890 + +0.3 950,890 + -0.3 950,890 + -0.6 950,891 - -0.8 950,891 - +0.2 950,891 - +1.5 950,891 - +1.0 950,891 - -0.8 950,894 + -0.8 950,894 + +2.0 950,894 + +1.0 950,894 + -1.2 950,895 - -1.3 950,895 - -0.9 950,895 - -0.6 950,895 - -1.0 950,895 - +0.4 950,915 - -1.0 950,927 - +0.7 950,927 - +2.0 950,927 - -1.8 950,927 - -0.0 951,106 + Pf1N1B4_829 0.31 -0.4 951,107 - Pf1N1B4_829 0.31 +2.0 951,265 + Pf1N1B4_829 0.50 -0.3 951,265 + Pf1N1B4_829 0.50 +1.3 951,266 - Pf1N1B4_829 0.50 +1.4 951,266 - Pf1N1B4_829 0.50 -0.0 951,266 - Pf1N1B4_829 0.50 +0.8 951,292 + Pf1N1B4_829 0.54 +1.1 951,292 + Pf1N1B4_829 0.54 +0.6 951,292 + Pf1N1B4_829 0.54 -1.8 951,292 + +1.6 951,293 - Pf1N1B4_829 0.54 -1.6 951,293 - Pf1N1B4_829 0.54 -1.0 951,293 - Pf1N1B4_829 0.54 +0.3 951,293 - Pf1N1B4_829 0.54 -0.8 951,293 - Pf1N1B4_829 0.54 -0.2 951,293 - Pf1N1B4_829 0.54 +2.0 951,293 - Pf1N1B4_829 0.54 +0.6
Or see this region's nucleotide sequence