Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_5833

Experiment: Cholin acetate 20 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_5832 and Pf1N1B4_5833 are separated by 243 nucleotidesPf1N1B4_5833 and Pf1N1B4_5834 are separated by 36 nucleotidesPf1N1B4_5834 and Pf1N1B4_5835 are separated by 11 nucleotides Pf1N1B4_5832 - LysR family transcriptional regulator near succinyl-CoA:3-ketoacid-coenzyme A transferase, at 266,702 to 267,601 _5832 Pf1N1B4_5833 - Succinyl-CoA:3-ketoacid-coenzyme A transferase subunit A (EC 2.8.3.5), at 267,845 to 268,543 _5833 Pf1N1B4_5834 - Succinyl-CoA:3-ketoacid-coenzyme A transferase subunit B (EC 2.8.3.5), at 268,580 to 269,245 _5834 Pf1N1B4_5835 - 3-ketoacyl-CoA thiolase (EC 2.3.1.16) @ Acetyl-CoA acetyltransferase (EC 2.3.1.9), at 269,257 to 270,438 _5835 Position (kb) 267 268 269Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 267.026 kb on - strand, within Pf1N1B4_5832at 267.120 kb on + strand, within Pf1N1B4_5832at 267.121 kb on - strand, within Pf1N1B4_5832at 267.173 kb on + strand, within Pf1N1B4_5832at 267.831 kb on + strandat 267.832 kb on - strandat 267.832 kb on - strandat 267.832 kb on - strandat 267.832 kb on - strandat 267.844 kb on + strandat 267.845 kb on - strandat 267.845 kb on - strandat 267.870 kb on - strandat 268.083 kb on - strand, within Pf1N1B4_5833at 268.275 kb on - strand, within Pf1N1B4_5833at 268.478 kb on + strandat 268.478 kb on + strandat 268.478 kb on + strandat 268.478 kb on + strandat 268.479 kb on - strandat 268.479 kb on - strandat 268.572 kb on + strandat 268.640 kb on + strandat 268.640 kb on + strandat 268.640 kb on + strandat 268.641 kb on - strandat 268.682 kb on + strand, within Pf1N1B4_5834at 268.682 kb on + strand, within Pf1N1B4_5834at 268.683 kb on - strand, within Pf1N1B4_5834at 268.683 kb on - strand, within Pf1N1B4_5834at 268.683 kb on - strand, within Pf1N1B4_5834at 269.423 kb on + strand, within Pf1N1B4_5835

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Per-strain Table

Position Strand Gene LocusTag Fraction Cholin acetate 20 mM
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267,026 - Pf1N1B4_5832 0.36 +0.4
267,120 + Pf1N1B4_5832 0.46 +0.5
267,121 - Pf1N1B4_5832 0.47 +0.5
267,173 + Pf1N1B4_5832 0.52 +0.5
267,831 + -0.5
267,832 - -1.6
267,832 - +1.0
267,832 - +0.5
267,832 - +0.3
267,844 + +0.5
267,845 - -1.3
267,845 - -2.0
267,870 - +0.5
268,083 - Pf1N1B4_5833 0.34 -0.5
268,275 - Pf1N1B4_5833 0.62 -2.0
268,478 + -2.3
268,478 + +1.1
268,478 + +0.5
268,478 + +2.5
268,479 - -0.8
268,479 - +0.5
268,572 + +2.7
268,640 + +0.5
268,640 + +0.2
268,640 + +1.0
268,641 - -1.0
268,682 + Pf1N1B4_5834 0.15 -0.5
268,682 + Pf1N1B4_5834 0.15 +0.5
268,683 - Pf1N1B4_5834 0.15 +1.0
268,683 - Pf1N1B4_5834 0.15 -3.2
268,683 - Pf1N1B4_5834 0.15 +0.2
269,423 + Pf1N1B4_5835 0.14 -0.8

Or see this region's nucleotide sequence