Experiment: Cholin acetate 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5459 and Pf1N1B4_5460 are separated by 173 nucleotides Pf1N1B4_5460 and Pf1N1B4_5461 are separated by 307 nucleotides
Pf1N1B4_5459 - possible integrase-like protein, at 205,846 to 206,937
_5459
Pf1N1B4_5460 - TolA protein, at 207,111 to 207,977
_5460
Pf1N1B4_5461 - hypothetical protein, at 208,285 to 208,695
_5461
Position (kb)
207
208 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 206.169 kb on + strand, within Pf1N1B4_5459 at 206.169 kb on + strand, within Pf1N1B4_5459 at 206.169 kb on + strand, within Pf1N1B4_5459 at 206.170 kb on - strand, within Pf1N1B4_5459 at 206.170 kb on - strand, within Pf1N1B4_5459 at 206.170 kb on - strand, within Pf1N1B4_5459 at 206.170 kb on - strand at 206.270 kb on + strand, within Pf1N1B4_5459 at 206.270 kb on + strand, within Pf1N1B4_5459 at 206.317 kb on - strand, within Pf1N1B4_5459 at 206.317 kb on - strand, within Pf1N1B4_5459 at 206.317 kb on - strand, within Pf1N1B4_5459 at 206.317 kb on - strand, within Pf1N1B4_5459 at 206.379 kb on + strand, within Pf1N1B4_5459 at 206.379 kb on + strand, within Pf1N1B4_5459 at 206.381 kb on + strand, within Pf1N1B4_5459 at 206.381 kb on + strand, within Pf1N1B4_5459 at 206.382 kb on - strand, within Pf1N1B4_5459 at 206.382 kb on - strand, within Pf1N1B4_5459 at 206.382 kb on - strand, within Pf1N1B4_5459 at 206.382 kb on - strand, within Pf1N1B4_5459 at 206.382 kb on - strand, within Pf1N1B4_5459 at 206.382 kb on - strand, within Pf1N1B4_5459 at 206.404 kb on + strand, within Pf1N1B4_5459 at 206.405 kb on - strand, within Pf1N1B4_5459 at 206.405 kb on - strand, within Pf1N1B4_5459 at 206.456 kb on - strand, within Pf1N1B4_5459 at 206.456 kb on - strand, within Pf1N1B4_5459 at 206.505 kb on + strand at 206.555 kb on + strand, within Pf1N1B4_5459 at 206.575 kb on - strand, within Pf1N1B4_5459 at 206.656 kb on - strand, within Pf1N1B4_5459 at 206.703 kb on + strand, within Pf1N1B4_5459 at 206.704 kb on - strand, within Pf1N1B4_5459 at 206.704 kb on - strand, within Pf1N1B4_5459 at 206.704 kb on - strand, within Pf1N1B4_5459 at 206.704 kb on - strand, within Pf1N1B4_5459 at 206.704 kb on - strand, within Pf1N1B4_5459 at 206.720 kb on + strand, within Pf1N1B4_5459 at 206.721 kb on - strand, within Pf1N1B4_5459 at 206.721 kb on - strand, within Pf1N1B4_5459 at 206.721 kb on - strand, within Pf1N1B4_5459 at 206.721 kb on - strand, within Pf1N1B4_5459 at 206.744 kb on + strand at 206.744 kb on + strand, within Pf1N1B4_5459 at 206.744 kb on + strand, within Pf1N1B4_5459 at 206.745 kb on - strand, within Pf1N1B4_5459 at 206.819 kb on + strand at 206.832 kb on + strand at 206.832 kb on + strand at 206.833 kb on - strand at 206.859 kb on - strand at 206.859 kb on - strand at 206.859 kb on - strand at 206.859 kb on - strand at 206.859 kb on - strand at 206.859 kb on - strand at 206.938 kb on - strand at 206.938 kb on - strand at 207.013 kb on + strand at 207.013 kb on + strand at 207.014 kb on - strand at 207.014 kb on - strand at 207.014 kb on - strand at 207.014 kb on - strand at 207.050 kb on - strand at 207.194 kb on + strand at 207.195 kb on - strand at 207.195 kb on - strand at 207.195 kb on - strand at 207.245 kb on + strand, within Pf1N1B4_5460 at 207.246 kb on - strand at 207.515 kb on + strand, within Pf1N1B4_5460 at 207.516 kb on - strand, within Pf1N1B4_5460 at 207.935 kb on + strand at 208.050 kb on + strand at 208.051 kb on - strand at 208.051 kb on - strand at 208.051 kb on - strand at 208.051 kb on - strand at 208.070 kb on + strand at 208.071 kb on - strand at 208.071 kb on - strand at 208.071 kb on - strand at 208.071 kb on - strand at 208.071 kb on - strand at 208.273 kb on - strand at 208.276 kb on + strand at 208.276 kb on + strand at 208.324 kb on + strand at 208.324 kb on + strand at 208.325 kb on - strand at 208.325 kb on - strand at 208.377 kb on - strand, within Pf1N1B4_5461 at 208.377 kb on - strand, within Pf1N1B4_5461 at 208.377 kb on - strand, within Pf1N1B4_5461 at 208.377 kb on - strand, within Pf1N1B4_5461 at 208.411 kb on + strand, within Pf1N1B4_5461 at 208.412 kb on - strand, within Pf1N1B4_5461 at 208.526 kb on - strand, within Pf1N1B4_5461 at 208.553 kb on - strand, within Pf1N1B4_5461 at 208.553 kb on - strand, within Pf1N1B4_5461 at 208.557 kb on - strand, within Pf1N1B4_5461 at 208.557 kb on - strand, within Pf1N1B4_5461 at 208.618 kb on - strand at 208.667 kb on + strand at 208.668 kb on - strand at 208.726 kb on + strand at 208.727 kb on - strand at 208.727 kb on - strand at 208.727 kb on - strand at 208.727 kb on - strand at 208.918 kb on + strand at 208.918 kb on + strand at 208.933 kb on - strand at 208.933 kb on - strand at 208.972 kb on - strand at 208.972 kb on - strand at 208.972 kb on - strand at 208.975 kb on + strand at 208.976 kb on - strand at 208.976 kb on - strand at 208.976 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cholin acetate 20 mM remove 206,169 + Pf1N1B4_5459 0.30 +1.3 206,169 + Pf1N1B4_5459 0.30 -0.2 206,169 + Pf1N1B4_5459 0.30 -1.4 206,170 - Pf1N1B4_5459 0.30 +0.5 206,170 - Pf1N1B4_5459 0.30 +0.4 206,170 - Pf1N1B4_5459 0.30 +0.4 206,170 - +0.9 206,270 + Pf1N1B4_5459 0.39 +0.5 206,270 + Pf1N1B4_5459 0.39 +0.5 206,317 - Pf1N1B4_5459 0.43 -0.9 206,317 - Pf1N1B4_5459 0.43 -1.0 206,317 - Pf1N1B4_5459 0.43 -0.1 206,317 - Pf1N1B4_5459 0.43 -0.1 206,379 + Pf1N1B4_5459 0.49 -0.1 206,379 + Pf1N1B4_5459 0.49 +0.9 206,381 + Pf1N1B4_5459 0.49 -1.1 206,381 + Pf1N1B4_5459 0.49 -0.1 206,382 - Pf1N1B4_5459 0.49 -0.6 206,382 - Pf1N1B4_5459 0.49 -0.9 206,382 - Pf1N1B4_5459 0.49 -0.2 206,382 - Pf1N1B4_5459 0.49 -1.1 206,382 - Pf1N1B4_5459 0.49 -0.5 206,382 - Pf1N1B4_5459 0.49 +0.9 206,404 + Pf1N1B4_5459 0.51 -0.2 206,405 - Pf1N1B4_5459 0.51 -0.1 206,405 - Pf1N1B4_5459 0.51 +0.1 206,456 - Pf1N1B4_5459 0.56 -0.4 206,456 - Pf1N1B4_5459 0.56 -0.9 206,505 + +0.9 206,555 + Pf1N1B4_5459 0.65 -1.0 206,575 - Pf1N1B4_5459 0.67 -1.4 206,656 - Pf1N1B4_5459 0.74 -0.9 206,703 + Pf1N1B4_5459 0.78 -1.4 206,704 - Pf1N1B4_5459 0.79 -1.0 206,704 - Pf1N1B4_5459 0.79 -0.6 206,704 - Pf1N1B4_5459 0.79 +0.2 206,704 - Pf1N1B4_5459 0.79 +0.5 206,704 - Pf1N1B4_5459 0.79 +0.2 206,720 + Pf1N1B4_5459 0.80 -0.1 206,721 - Pf1N1B4_5459 0.80 +0.9 206,721 - Pf1N1B4_5459 0.80 +0.5 206,721 - Pf1N1B4_5459 0.80 -0.2 206,721 - Pf1N1B4_5459 0.80 -1.1 206,744 + -0.4 206,744 + Pf1N1B4_5459 0.82 +0.9 206,744 + Pf1N1B4_5459 0.82 +0.9 206,745 - Pf1N1B4_5459 0.82 +1.9 206,819 + -0.6 206,832 + +0.9 206,832 + -1.1 206,833 - -1.1 206,859 - -1.2 206,859 - -0.6 206,859 - -1.4 206,859 - -0.7 206,859 - +0.1 206,859 - -1.1 206,938 - +0.7 206,938 - +1.2 207,013 + +1.3 207,013 + -0.1 207,014 - +0.4 207,014 - +0.5 207,014 - +0.5 207,014 - +0.5 207,050 - -1.1 207,194 + -2.0 207,195 - -1.8 207,195 - +2.3 207,195 - +1.5 207,245 + Pf1N1B4_5460 0.15 -0.2 207,246 - +0.9 207,515 + Pf1N1B4_5460 0.47 -1.6 207,516 - Pf1N1B4_5460 0.47 -0.8 207,935 + -1.1 208,050 + +0.2 208,051 - -0.5 208,051 - +0.8 208,051 - +0.3 208,051 - +1.4 208,070 + +0.5 208,071 - +2.4 208,071 - -0.0 208,071 - -1.1 208,071 - +0.2 208,071 - +0.5 208,273 - -0.4 208,276 + -1.2 208,276 + -1.1 208,324 + -0.1 208,324 + -0.3 208,325 - +0.1 208,325 - +0.1 208,377 - Pf1N1B4_5461 0.22 +1.3 208,377 - Pf1N1B4_5461 0.22 -0.8 208,377 - Pf1N1B4_5461 0.22 +0.7 208,377 - Pf1N1B4_5461 0.22 -0.5 208,411 + Pf1N1B4_5461 0.31 +3.3 208,412 - Pf1N1B4_5461 0.31 +0.9 208,526 - Pf1N1B4_5461 0.59 +0.1 208,553 - Pf1N1B4_5461 0.65 +0.7 208,553 - Pf1N1B4_5461 0.65 -0.2 208,557 - Pf1N1B4_5461 0.66 -2.0 208,557 - Pf1N1B4_5461 0.66 -1.8 208,618 - +0.9 208,667 + -0.1 208,668 - -1.4 208,726 + -0.1 208,727 - -0.3 208,727 - -0.1 208,727 - -1.6 208,727 - -0.3 208,918 + +2.5 208,918 + -0.1 208,933 - -0.1 208,933 - +0.4 208,972 - -0.6 208,972 - -0.5 208,972 - -1.6 208,975 + -0.6 208,976 - -0.3 208,976 - -0.4 208,976 - +0.1
Or see this region's nucleotide sequence