Experiment: Cholin acetate 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_528 and Pf1N1B4_529 are separated by 82 nucleotides Pf1N1B4_529 and Pf1N1B4_530 are separated by 78 nucleotides Pf1N1B4_530 and Pf1N1B4_531 are separated by 186 nucleotides Pf1N1B4_531 and Pf1N1B4_532 are separated by 140 nucleotides
Pf1N1B4_528 - sodium-solute symporter, putative, at 627,690 to 629,069
_528
Pf1N1B4_529 - SoxH protein, homolog, at 629,152 to 629,877
_529
Pf1N1B4_530 - hypothetical protein, at 629,956 to 630,180
_530
Pf1N1B4_531 - hypothetical protein, at 630,367 to 630,495
_531
Pf1N1B4_532 - Transcriptional regulators, LysR family, at 630,636 to 631,529
_532
Position (kb) 629
630 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 628.176 kb on + strand, within Pf1N1B4_528 at 628.177 kb on - strand, within Pf1N1B4_528 at 628.278 kb on + strand, within Pf1N1B4_528 at 628.368 kb on + strand, within Pf1N1B4_528 at 628.368 kb on + strand, within Pf1N1B4_528 at 628.369 kb on - strand, within Pf1N1B4_528 at 628.369 kb on - strand, within Pf1N1B4_528 at 628.380 kb on + strand at 628.380 kb on + strand, within Pf1N1B4_528 at 628.380 kb on + strand at 628.464 kb on - strand, within Pf1N1B4_528 at 628.479 kb on + strand, within Pf1N1B4_528 at 628.479 kb on + strand, within Pf1N1B4_528 at 628.479 kb on + strand, within Pf1N1B4_528 at 628.601 kb on + strand, within Pf1N1B4_528 at 628.601 kb on + strand, within Pf1N1B4_528 at 628.827 kb on + strand, within Pf1N1B4_528 at 628.827 kb on + strand, within Pf1N1B4_528 at 628.827 kb on + strand, within Pf1N1B4_528 at 628.827 kb on + strand, within Pf1N1B4_528 at 628.828 kb on - strand, within Pf1N1B4_528 at 628.983 kb on + strand at 628.983 kb on + strand at 628.983 kb on + strand at 628.983 kb on + strand at 628.984 kb on - strand at 628.984 kb on - strand at 628.984 kb on - strand at 628.986 kb on + strand at 628.987 kb on - strand at 628.987 kb on - strand at 629.067 kb on + strand at 629.073 kb on + strand at 629.074 kb on - strand at 629.152 kb on - strand at 629.227 kb on + strand, within Pf1N1B4_529 at 629.227 kb on + strand at 629.227 kb on + strand, within Pf1N1B4_529 at 629.227 kb on + strand, within Pf1N1B4_529 at 629.227 kb on + strand, within Pf1N1B4_529 at 629.228 kb on - strand at 629.228 kb on - strand at 629.228 kb on - strand, within Pf1N1B4_529 at 629.228 kb on - strand, within Pf1N1B4_529 at 629.232 kb on + strand, within Pf1N1B4_529 at 629.232 kb on + strand, within Pf1N1B4_529 at 629.233 kb on - strand, within Pf1N1B4_529 at 629.243 kb on - strand, within Pf1N1B4_529 at 629.704 kb on + strand at 629.705 kb on - strand, within Pf1N1B4_529 at 629.705 kb on - strand, within Pf1N1B4_529 at 629.732 kb on - strand, within Pf1N1B4_529 at 629.732 kb on - strand, within Pf1N1B4_529 at 629.753 kb on - strand, within Pf1N1B4_529 at 629.887 kb on + strand at 629.887 kb on + strand at 629.888 kb on - strand at 629.917 kb on - strand at 629.917 kb on - strand at 629.924 kb on + strand at 629.974 kb on + strand at 629.974 kb on + strand at 629.974 kb on + strand at 629.974 kb on + strand at 629.974 kb on + strand at 629.975 kb on - strand at 629.975 kb on - strand at 629.975 kb on - strand at 629.975 kb on - strand at 629.975 kb on - strand at 630.046 kb on - strand, within Pf1N1B4_530 at 630.195 kb on + strand at 630.557 kb on + strand at 630.558 kb on - strand at 630.558 kb on - strand at 630.570 kb on + strand at 630.607 kb on + strand at 630.607 kb on + strand at 630.674 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cholin acetate 20 mM remove 628,176 + Pf1N1B4_528 0.35 -0.6 628,177 - Pf1N1B4_528 0.35 -0.0 628,278 + Pf1N1B4_528 0.43 -1.1 628,368 + Pf1N1B4_528 0.49 +1.4 628,368 + Pf1N1B4_528 0.49 -0.5 628,369 - Pf1N1B4_528 0.49 -1.1 628,369 - Pf1N1B4_528 0.49 +0.5 628,380 + -0.7 628,380 + Pf1N1B4_528 0.50 -0.7 628,380 + +0.9 628,464 - Pf1N1B4_528 0.56 +0.9 628,479 + Pf1N1B4_528 0.57 +0.4 628,479 + Pf1N1B4_528 0.57 -0.1 628,479 + Pf1N1B4_528 0.57 +0.9 628,601 + Pf1N1B4_528 0.66 +1.2 628,601 + Pf1N1B4_528 0.66 +0.5 628,827 + Pf1N1B4_528 0.82 -0.7 628,827 + Pf1N1B4_528 0.82 +2.7 628,827 + Pf1N1B4_528 0.82 +0.9 628,827 + Pf1N1B4_528 0.82 -0.5 628,828 - Pf1N1B4_528 0.82 -0.4 628,983 + -0.7 628,983 + +0.5 628,983 + -1.5 628,983 + -0.7 628,984 - +1.3 628,984 - +0.5 628,984 - +2.0 628,986 + -0.0 628,987 - +1.3 628,987 - +0.3 629,067 + -0.0 629,073 + -1.5 629,074 - +0.1 629,152 - -0.4 629,227 + Pf1N1B4_529 0.10 -1.0 629,227 + +3.4 629,227 + Pf1N1B4_529 0.10 -1.6 629,227 + Pf1N1B4_529 0.10 -0.4 629,227 + Pf1N1B4_529 0.10 -2.3 629,228 - -1.1 629,228 - +1.9 629,228 - Pf1N1B4_529 0.10 -1.0 629,228 - Pf1N1B4_529 0.10 +0.0 629,232 + Pf1N1B4_529 0.11 +0.7 629,232 + Pf1N1B4_529 0.11 +0.1 629,233 - Pf1N1B4_529 0.11 -2.1 629,243 - Pf1N1B4_529 0.13 -0.5 629,704 + +1.9 629,705 - Pf1N1B4_529 0.76 -0.5 629,705 - Pf1N1B4_529 0.76 +0.9 629,732 - Pf1N1B4_529 0.80 -0.2 629,732 - Pf1N1B4_529 0.80 -1.0 629,753 - Pf1N1B4_529 0.83 -0.9 629,887 + +1.0 629,887 + +0.3 629,888 - -0.1 629,917 - +0.4 629,917 - +0.6 629,924 + +0.9 629,974 + -0.4 629,974 + +0.9 629,974 + -0.3 629,974 + +0.9 629,974 + -0.1 629,975 - -0.1 629,975 - -1.1 629,975 - +1.3 629,975 - +0.9 629,975 - +0.9 630,046 - Pf1N1B4_530 0.40 -0.7 630,195 + +0.3 630,557 + -0.1 630,558 - +0.3 630,558 - -1.3 630,570 + -0.4 630,607 + -1.1 630,607 + -2.3 630,674 - -0.2
Or see this region's nucleotide sequence