Experiment: Cholin acetate 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4606 and Pf1N1B4_4607 are separated by 22 nucleotides Pf1N1B4_4607 and Pf1N1B4_4608 overlap by 25 nucleotides Pf1N1B4_4608 and Pf1N1B4_4609 overlap by 1 nucleotides Pf1N1B4_4609 and Pf1N1B4_4610 overlap by 31 nucleotides Pf1N1B4_4610 and Pf1N1B4_4611 overlap by 4 nucleotides
Pf1N1B4_4606 - NAD kinase (EC 2.7.1.23), at 5,079,044 to 5,079,364
_4606
Pf1N1B4_4607 - NAD kinase (EC 2.7.1.23), at 5,079,387 to 5,079,728
_4607
Pf1N1B4_4608 - NAD kinase (EC 2.7.1.23), at 5,079,704 to 5,079,934
_4608
Pf1N1B4_4609 - Diadenosine tetraphosphatase and related serine/threonine protein phosphatases, at 5,079,934 to 5,080,398
_4609
Pf1N1B4_4610 - Diadenosine tetraphosphatase and related serine/threonine protein phosphatases, at 5,080,368 to 5,080,904
_4610
Pf1N1B4_4611 - GlpG protein (membrane protein of glp regulon), at 5,080,901 to 5,081,773
_4611
Position (kb)
5079
5080
5081 Strain fitness (log2 ratio)
-1
0
1
2 at 5079.951 kb on + strand at 5079.956 kb on - strand at 5080.243 kb on - strand, within Pf1N1B4_4609 at 5080.329 kb on + strand, within Pf1N1B4_4609 at 5080.701 kb on + strand, within Pf1N1B4_4610 at 5080.737 kb on + strand, within Pf1N1B4_4610 at 5080.859 kb on + strand at 5080.920 kb on + strand at 5080.920 kb on + strand at 5080.920 kb on + strand at 5080.920 kb on + strand at 5080.921 kb on - strand at 5081.019 kb on + strand, within Pf1N1B4_4611 at 5081.136 kb on + strand, within Pf1N1B4_4611
Per-strain Table
Position Strand Gene LocusTag Fraction Cholin acetate 20 mM remove 5,079,951 + +0.0 5,079,956 - -0.3 5,080,243 - Pf1N1B4_4609 0.66 +1.2 5,080,329 + Pf1N1B4_4609 0.85 -0.7 5,080,701 + Pf1N1B4_4610 0.62 -0.9 5,080,737 + Pf1N1B4_4610 0.69 -0.5 5,080,859 + +0.5 5,080,920 + +1.8 5,080,920 + +0.5 5,080,920 + -0.4 5,080,920 + -0.9 5,080,921 - -0.8 5,081,019 + Pf1N1B4_4611 0.14 -0.8 5,081,136 + Pf1N1B4_4611 0.27 +0.5
Or see this region's nucleotide sequence