Experiment: Cholin acetate 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4350 and Pf1N1B4_4351 overlap by 4 nucleotides Pf1N1B4_4351 and Pf1N1B4_4352 overlap by 4 nucleotides Pf1N1B4_4352 and Pf1N1B4_4353 are separated by 350 nucleotides
Pf1N1B4_4350 - Putrescine transport system permease protein PotI (TC 3.A.1.11.2), at 4,806,194 to 4,807,078
_4350
Pf1N1B4_4351 - Putrescine transport system permease protein PotH (TC 3.A.1.11.2), at 4,807,075 to 4,807,989
_4351
Pf1N1B4_4352 - Putrescine transport ATP-binding protein PotG (TC 3.A.1.11.2), at 4,807,986 to 4,809,128
_4352
Pf1N1B4_4353 - Gamma-glutamyl-GABA hydrolase (EC 3.5.1.94), at 4,809,479 to 4,810,240
_4353
Position (kb)
4807
4808
4809
4810 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 4807.024 kb on - strand at 4807.024 kb on - strand at 4807.028 kb on - strand at 4807.445 kb on + strand, within Pf1N1B4_4351 at 4807.446 kb on - strand, within Pf1N1B4_4351 at 4807.446 kb on - strand, within Pf1N1B4_4351 at 4807.452 kb on - strand, within Pf1N1B4_4351 at 4807.452 kb on - strand, within Pf1N1B4_4351 at 4807.467 kb on - strand, within Pf1N1B4_4351 at 4807.467 kb on - strand, within Pf1N1B4_4351 at 4807.467 kb on - strand, within Pf1N1B4_4351 at 4807.664 kb on + strand, within Pf1N1B4_4351 at 4807.665 kb on - strand, within Pf1N1B4_4351 at 4807.665 kb on - strand at 4807.745 kb on + strand, within Pf1N1B4_4351 at 4807.745 kb on + strand, within Pf1N1B4_4351 at 4807.746 kb on - strand, within Pf1N1B4_4351 at 4807.746 kb on - strand, within Pf1N1B4_4351 at 4807.746 kb on - strand, within Pf1N1B4_4351 at 4807.746 kb on - strand, within Pf1N1B4_4351 at 4807.773 kb on - strand, within Pf1N1B4_4351 at 4807.820 kb on + strand, within Pf1N1B4_4351 at 4807.832 kb on + strand, within Pf1N1B4_4351 at 4807.841 kb on - strand at 4808.029 kb on + strand at 4808.030 kb on - strand at 4808.177 kb on + strand, within Pf1N1B4_4352 at 4808.233 kb on + strand, within Pf1N1B4_4352 at 4808.234 kb on - strand, within Pf1N1B4_4352 at 4808.234 kb on - strand, within Pf1N1B4_4352 at 4808.407 kb on + strand, within Pf1N1B4_4352 at 4808.407 kb on + strand, within Pf1N1B4_4352 at 4808.408 kb on - strand, within Pf1N1B4_4352 at 4808.408 kb on - strand, within Pf1N1B4_4352 at 4809.106 kb on + strand at 4809.106 kb on + strand at 4809.106 kb on + strand at 4809.107 kb on - strand at 4809.107 kb on - strand at 4809.176 kb on + strand at 4809.176 kb on + strand at 4809.176 kb on + strand at 4809.281 kb on - strand at 4809.376 kb on + strand at 4809.377 kb on - strand at 4809.400 kb on + strand at 4809.401 kb on - strand at 4809.403 kb on + strand at 4809.403 kb on + strand at 4809.404 kb on - strand at 4809.404 kb on - strand at 4809.455 kb on + strand at 4809.456 kb on - strand at 4809.488 kb on - strand at 4809.538 kb on + strand at 4809.546 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cholin acetate 20 mM remove 4,807,024 - -1.8 4,807,024 - +0.2 4,807,028 - +0.6 4,807,445 + Pf1N1B4_4351 0.40 +1.0 4,807,446 - Pf1N1B4_4351 0.41 +0.4 4,807,446 - Pf1N1B4_4351 0.41 +0.3 4,807,452 - Pf1N1B4_4351 0.41 +0.5 4,807,452 - Pf1N1B4_4351 0.41 -0.0 4,807,467 - Pf1N1B4_4351 0.43 +0.2 4,807,467 - Pf1N1B4_4351 0.43 +0.4 4,807,467 - Pf1N1B4_4351 0.43 +0.0 4,807,664 + Pf1N1B4_4351 0.64 +0.1 4,807,665 - Pf1N1B4_4351 0.64 +1.3 4,807,665 - -0.8 4,807,745 + Pf1N1B4_4351 0.73 -2.4 4,807,745 + Pf1N1B4_4351 0.73 -0.4 4,807,746 - Pf1N1B4_4351 0.73 -0.0 4,807,746 - Pf1N1B4_4351 0.73 +0.8 4,807,746 - Pf1N1B4_4351 0.73 -0.4 4,807,746 - Pf1N1B4_4351 0.73 +0.6 4,807,773 - Pf1N1B4_4351 0.76 -1.6 4,807,820 + Pf1N1B4_4351 0.81 +0.6 4,807,832 + Pf1N1B4_4351 0.83 -0.3 4,807,841 - +1.0 4,808,029 + -0.2 4,808,030 - +0.6 4,808,177 + Pf1N1B4_4352 0.17 -0.2 4,808,233 + Pf1N1B4_4352 0.22 -1.0 4,808,234 - Pf1N1B4_4352 0.22 -0.8 4,808,234 - Pf1N1B4_4352 0.22 +0.2 4,808,407 + Pf1N1B4_4352 0.37 -2.0 4,808,407 + Pf1N1B4_4352 0.37 +0.6 4,808,408 - Pf1N1B4_4352 0.37 -0.4 4,808,408 - Pf1N1B4_4352 0.37 -0.7 4,809,106 + +3.2 4,809,106 + +2.6 4,809,106 + +0.1 4,809,107 - +0.6 4,809,107 - -0.4 4,809,176 + +0.2 4,809,176 + +0.3 4,809,176 + +0.1 4,809,281 - -0.2 4,809,376 + +1.2 4,809,377 - -1.0 4,809,400 + -0.0 4,809,401 - -1.8 4,809,403 + -0.1 4,809,403 + +2.7 4,809,404 - -1.6 4,809,404 - +0.2 4,809,455 + -1.4 4,809,456 - +1.4 4,809,488 - -0.4 4,809,538 + -0.4 4,809,546 + +0.2
Or see this region's nucleotide sequence