Experiment: Cholin acetate 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3414 and Pf1N1B4_3415 are separated by 141 nucleotides Pf1N1B4_3415 and Pf1N1B4_3416 are separated by 215 nucleotides Pf1N1B4_3416 and Pf1N1B4_3417 are separated by 359 nucleotides Pf1N1B4_3417 and Pf1N1B4_3418 are separated by 138 nucleotides Pf1N1B4_3418 and Pf1N1B4_3419 are separated by 23 nucleotides
Pf1N1B4_3414 - PROBABLE MEMBRANE PROTEIN, at 3,804,659 to 3,804,907
_3414
Pf1N1B4_3415 - Transcriptional regulator, TetR family, at 3,805,049 to 3,805,657
_3415
Pf1N1B4_3416 - Outer membrane protein romA, at 3,805,873 to 3,806,931
_3416
Pf1N1B4_3417 - FIG00964635: hypothetical protein, at 3,807,291 to 3,807,608
_3417
Pf1N1B4_3418 - hypothetical protein, at 3,807,747 to 3,807,860
_3418
Pf1N1B4_3419 - Periplasmic beta-glucosidase (EC 3.2.1.21), at 3,807,884 to 3,810,175
_3419
Position (kb)
3805
3806
3807 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3804.944 kb on + strand at 3804.945 kb on - strand at 3804.946 kb on + strand at 3804.947 kb on - strand at 3804.990 kb on + strand at 3804.991 kb on - strand at 3804.991 kb on - strand at 3804.995 kb on - strand at 3805.060 kb on - strand at 3805.060 kb on - strand at 3805.248 kb on - strand, within Pf1N1B4_3415 at 3805.515 kb on + strand, within Pf1N1B4_3415 at 3805.515 kb on + strand, within Pf1N1B4_3415 at 3805.515 kb on + strand, within Pf1N1B4_3415 at 3805.516 kb on - strand, within Pf1N1B4_3415 at 3805.516 kb on - strand, within Pf1N1B4_3415 at 3805.516 kb on - strand, within Pf1N1B4_3415 at 3805.528 kb on + strand, within Pf1N1B4_3415 at 3805.657 kb on + strand at 3805.657 kb on + strand at 3805.661 kb on + strand at 3805.661 kb on + strand at 3805.661 kb on + strand at 3805.704 kb on - strand at 3805.737 kb on - strand at 3805.737 kb on - strand at 3805.750 kb on + strand at 3805.761 kb on + strand at 3805.818 kb on + strand at 3805.819 kb on - strand at 3805.819 kb on - strand at 3805.884 kb on - strand at 3805.955 kb on + strand at 3805.956 kb on - strand at 3806.127 kb on - strand, within Pf1N1B4_3416 at 3806.127 kb on - strand, within Pf1N1B4_3416 at 3806.284 kb on + strand, within Pf1N1B4_3416 at 3806.284 kb on + strand, within Pf1N1B4_3416 at 3806.284 kb on + strand, within Pf1N1B4_3416 at 3806.285 kb on - strand, within Pf1N1B4_3416 at 3806.285 kb on - strand, within Pf1N1B4_3416 at 3806.285 kb on - strand, within Pf1N1B4_3416 at 3806.335 kb on + strand, within Pf1N1B4_3416 at 3806.336 kb on - strand, within Pf1N1B4_3416 at 3806.641 kb on + strand, within Pf1N1B4_3416 at 3806.641 kb on + strand, within Pf1N1B4_3416 at 3806.642 kb on - strand, within Pf1N1B4_3416 at 3806.707 kb on - strand, within Pf1N1B4_3416 at 3806.707 kb on - strand, within Pf1N1B4_3416 at 3806.784 kb on + strand, within Pf1N1B4_3416 at 3806.857 kb on + strand at 3806.857 kb on + strand at 3806.858 kb on - strand at 3806.858 kb on - strand at 3806.858 kb on - strand at 3806.983 kb on + strand at 3806.984 kb on - strand at 3806.984 kb on - strand at 3806.984 kb on - strand at 3806.999 kb on + strand at 3807.000 kb on - strand at 3807.026 kb on + strand at 3807.026 kb on + strand at 3807.027 kb on - strand at 3807.027 kb on - strand at 3807.027 kb on - strand at 3807.085 kb on + strand at 3807.085 kb on + strand at 3807.097 kb on + strand at 3807.098 kb on - strand at 3807.098 kb on - strand at 3807.189 kb on + strand at 3807.307 kb on + strand at 3807.308 kb on - strand at 3807.749 kb on - strand at 3807.894 kb on + strand at 3807.894 kb on + strand at 3807.894 kb on + strand at 3807.903 kb on + strand at 3807.903 kb on + strand at 3807.903 kb on + strand at 3807.903 kb on + strand at 3807.904 kb on - strand at 3807.904 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cholin acetate 20 mM remove 3,804,944 + -1.2 3,804,945 - +0.3 3,804,946 + +0.1 3,804,947 - +2.4 3,804,990 + -2.3 3,804,991 - -2.9 3,804,991 - -0.2 3,804,995 - +0.8 3,805,060 - -1.6 3,805,060 - +0.4 3,805,248 - Pf1N1B4_3415 0.33 +0.5 3,805,515 + Pf1N1B4_3415 0.77 -0.2 3,805,515 + Pf1N1B4_3415 0.77 +0.8 3,805,515 + Pf1N1B4_3415 0.77 -0.5 3,805,516 - Pf1N1B4_3415 0.77 -0.3 3,805,516 - Pf1N1B4_3415 0.77 -0.6 3,805,516 - Pf1N1B4_3415 0.77 +0.5 3,805,528 + Pf1N1B4_3415 0.79 -0.7 3,805,657 + -0.9 3,805,657 + -0.7 3,805,661 + +1.8 3,805,661 + +0.4 3,805,661 + -0.4 3,805,704 - -0.6 3,805,737 - -0.7 3,805,737 - -0.5 3,805,750 + -1.2 3,805,761 + -0.7 3,805,818 + -1.5 3,805,819 - +1.8 3,805,819 - +0.3 3,805,884 - -0.5 3,805,955 + +1.8 3,805,956 - +0.5 3,806,127 - Pf1N1B4_3416 0.24 -0.3 3,806,127 - Pf1N1B4_3416 0.24 -1.5 3,806,284 + Pf1N1B4_3416 0.39 -1.2 3,806,284 + Pf1N1B4_3416 0.39 +0.8 3,806,284 + Pf1N1B4_3416 0.39 -0.5 3,806,285 - Pf1N1B4_3416 0.39 -2.6 3,806,285 - Pf1N1B4_3416 0.39 -1.2 3,806,285 - Pf1N1B4_3416 0.39 -0.2 3,806,335 + Pf1N1B4_3416 0.44 -0.6 3,806,336 - Pf1N1B4_3416 0.44 +2.2 3,806,641 + Pf1N1B4_3416 0.73 +0.8 3,806,641 + Pf1N1B4_3416 0.73 -0.7 3,806,642 - Pf1N1B4_3416 0.73 -0.9 3,806,707 - Pf1N1B4_3416 0.79 -0.6 3,806,707 - Pf1N1B4_3416 0.79 +2.0 3,806,784 + Pf1N1B4_3416 0.86 +0.2 3,806,857 + -0.2 3,806,857 + +0.3 3,806,858 - +1.1 3,806,858 - +0.8 3,806,858 - -0.6 3,806,983 + +0.6 3,806,984 - +0.6 3,806,984 - -0.0 3,806,984 - -1.1 3,806,999 + +1.2 3,807,000 - -0.4 3,807,026 + -0.3 3,807,026 + +0.6 3,807,027 - +0.6 3,807,027 - +1.4 3,807,027 - +2.8 3,807,085 + -1.4 3,807,085 + -1.5 3,807,097 + -0.3 3,807,098 - -0.2 3,807,098 - +0.6 3,807,189 + +0.5 3,807,307 + +0.8 3,807,308 - -1.7 3,807,749 - +0.1 3,807,894 + -0.2 3,807,894 + -0.4 3,807,894 + +0.4 3,807,903 + +0.6 3,807,903 + -1.3 3,807,903 + +0.2 3,807,903 + +1.6 3,807,904 - -0.2 3,807,904 - -0.4
Or see this region's nucleotide sequence