Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_1506

Experiment: Cholin acetate 20 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_1504 and Pf1N1B4_1505 overlap by 4 nucleotidesPf1N1B4_1505 and Pf1N1B4_1506 are separated by 373 nucleotidesPf1N1B4_1506 and Pf1N1B4_1507 are separated by 164 nucleotidesPf1N1B4_1507 and Pf1N1B4_1508 overlap by 4 nucleotides Pf1N1B4_1504 - Acyl carrier protein, at 1,737,079 to 1,737,333 _1504 Pf1N1B4_1505 - FIG017861: hypothetical protein, at 1,737,330 to 1,737,875 _1505 Pf1N1B4_1506 - FIGfam138462: Acyl-CoA synthetase, AMP-(fatty) acid ligase / (3R)-hydroxymyristoyl-[ACP] dehydratase (EC 4.2.1.-), at 1,738,249 to 1,739,550 _1506 Pf1N1B4_1507 - FIG143263: Glycosyl transferase, at 1,739,715 to 1,740,449 _1507 Pf1N1B4_1508 - Lysophospholipid acyltransferase, at 1,740,446 to 1,741,393 _1508 Position (kb) 1738 1739 1740Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 1737.280 kb on - strand, within Pf1N1B4_1504at 1737.375 kb on + strandat 1737.376 kb on - strandat 1737.376 kb on - strandat 1737.376 kb on - strandat 1737.390 kb on + strand, within Pf1N1B4_1505at 1737.390 kb on + strand, within Pf1N1B4_1505at 1737.391 kb on - strand, within Pf1N1B4_1505at 1737.391 kb on - strand, within Pf1N1B4_1505at 1737.618 kb on + strand, within Pf1N1B4_1505at 1737.618 kb on + strand, within Pf1N1B4_1505at 1737.619 kb on - strand, within Pf1N1B4_1505at 1737.668 kb on - strand, within Pf1N1B4_1505at 1737.789 kb on + strand, within Pf1N1B4_1505at 1737.789 kb on + strand, within Pf1N1B4_1505at 1737.789 kb on + strand, within Pf1N1B4_1505at 1737.789 kb on + strand, within Pf1N1B4_1505at 1737.789 kb on + strand, within Pf1N1B4_1505at 1737.789 kb on + strand, within Pf1N1B4_1505at 1737.790 kb on - strand, within Pf1N1B4_1505at 1737.790 kb on - strand, within Pf1N1B4_1505at 1737.790 kb on - strand, within Pf1N1B4_1505at 1737.807 kb on + strand, within Pf1N1B4_1505at 1737.807 kb on + strand, within Pf1N1B4_1505at 1737.808 kb on - strand, within Pf1N1B4_1505at 1737.860 kb on - strandat 1738.089 kb on + strandat 1738.089 kb on + strandat 1738.089 kb on + strandat 1738.090 kb on - strandat 1738.393 kb on + strand, within Pf1N1B4_1506at 1738.393 kb on + strand, within Pf1N1B4_1506at 1738.393 kb on + strand, within Pf1N1B4_1506at 1738.394 kb on - strand, within Pf1N1B4_1506at 1738.771 kb on - strand, within Pf1N1B4_1506at 1738.783 kb on + strand, within Pf1N1B4_1506at 1738.783 kb on + strand, within Pf1N1B4_1506at 1738.784 kb on - strand, within Pf1N1B4_1506at 1738.897 kb on - strand, within Pf1N1B4_1506at 1738.897 kb on - strand, within Pf1N1B4_1506at 1738.988 kb on + strand, within Pf1N1B4_1506at 1739.004 kb on - strand, within Pf1N1B4_1506at 1739.056 kb on - strand, within Pf1N1B4_1506at 1739.136 kb on - strand, within Pf1N1B4_1506at 1739.282 kb on - strand, within Pf1N1B4_1506at 1739.282 kb on - strand, within Pf1N1B4_1506at 1739.479 kb on + strandat 1739.480 kb on - strandat 1739.480 kb on - strandat 1739.480 kb on - strandat 1739.529 kb on - strandat 1739.566 kb on - strandat 1739.624 kb on - strandat 1739.651 kb on - strandat 1739.651 kb on - strandat 1739.651 kb on - strandat 1739.721 kb on - strandat 1739.745 kb on + strandat 1739.745 kb on + strandat 1739.745 kb on + strandat 1739.745 kb on + strandat 1739.746 kb on - strandat 1739.746 kb on - strandat 1739.880 kb on + strand, within Pf1N1B4_1507at 1739.880 kb on + strand, within Pf1N1B4_1507at 1740.012 kb on - strand, within Pf1N1B4_1507at 1740.073 kb on - strand, within Pf1N1B4_1507at 1740.101 kb on - strand, within Pf1N1B4_1507at 1740.101 kb on - strand, within Pf1N1B4_1507at 1740.102 kb on + strandat 1740.103 kb on - strand, within Pf1N1B4_1507at 1740.103 kb on - strand, within Pf1N1B4_1507at 1740.103 kb on - strand, within Pf1N1B4_1507at 1740.187 kb on - strand, within Pf1N1B4_1507at 1740.240 kb on - strand, within Pf1N1B4_1507at 1740.240 kb on - strand, within Pf1N1B4_1507at 1740.364 kb on + strand, within Pf1N1B4_1507at 1740.364 kb on + strand, within Pf1N1B4_1507at 1740.365 kb on - strand, within Pf1N1B4_1507at 1740.365 kb on - strand, within Pf1N1B4_1507at 1740.365 kb on - strand, within Pf1N1B4_1507at 1740.365 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Cholin acetate 20 mM
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1,737,280 - Pf1N1B4_1504 0.79 -2.4
1,737,375 + +1.2
1,737,376 - +0.2
1,737,376 - -0.8
1,737,376 - +0.2
1,737,390 + Pf1N1B4_1505 0.11 +1.0
1,737,390 + Pf1N1B4_1505 0.11 +0.6
1,737,391 - Pf1N1B4_1505 0.11 +1.4
1,737,391 - Pf1N1B4_1505 0.11 -1.0
1,737,618 + Pf1N1B4_1505 0.53 -0.1
1,737,618 + Pf1N1B4_1505 0.53 -0.5
1,737,619 - Pf1N1B4_1505 0.53 +0.2
1,737,668 - Pf1N1B4_1505 0.62 +0.5
1,737,789 + Pf1N1B4_1505 0.84 +0.5
1,737,789 + Pf1N1B4_1505 0.84 +0.1
1,737,789 + Pf1N1B4_1505 0.84 -0.1
1,737,789 + Pf1N1B4_1505 0.84 +0.4
1,737,789 + Pf1N1B4_1505 0.84 +1.8
1,737,789 + Pf1N1B4_1505 0.84 +0.6
1,737,790 - Pf1N1B4_1505 0.84 +0.6
1,737,790 - Pf1N1B4_1505 0.84 -0.1
1,737,790 - Pf1N1B4_1505 0.84 -2.6
1,737,807 + Pf1N1B4_1505 0.87 +0.2
1,737,807 + Pf1N1B4_1505 0.87 -0.3
1,737,808 - Pf1N1B4_1505 0.88 -0.2
1,737,860 - +1.0
1,738,089 + +1.2
1,738,089 + -1.0
1,738,089 + +1.2
1,738,090 - -1.1
1,738,393 + Pf1N1B4_1506 0.11 -1.1
1,738,393 + Pf1N1B4_1506 0.11 +0.8
1,738,393 + Pf1N1B4_1506 0.11 -1.0
1,738,394 - Pf1N1B4_1506 0.11 -1.4
1,738,771 - Pf1N1B4_1506 0.40 -1.1
1,738,783 + Pf1N1B4_1506 0.41 -0.8
1,738,783 + Pf1N1B4_1506 0.41 -1.4
1,738,784 - Pf1N1B4_1506 0.41 -0.0
1,738,897 - Pf1N1B4_1506 0.50 +0.2
1,738,897 - Pf1N1B4_1506 0.50 +1.7
1,738,988 + Pf1N1B4_1506 0.57 -0.2
1,739,004 - Pf1N1B4_1506 0.58 -0.1
1,739,056 - Pf1N1B4_1506 0.62 -0.9
1,739,136 - Pf1N1B4_1506 0.68 +0.3
1,739,282 - Pf1N1B4_1506 0.79 -1.0
1,739,282 - Pf1N1B4_1506 0.79 -1.5
1,739,479 + -0.4
1,739,480 - +0.2
1,739,480 - +0.6
1,739,480 - -0.5
1,739,529 - -0.0
1,739,566 - -1.0
1,739,624 - +0.6
1,739,651 - -2.1
1,739,651 - -0.8
1,739,651 - +0.6
1,739,721 - +0.5
1,739,745 + +1.0
1,739,745 + -1.6
1,739,745 + -3.1
1,739,745 + +0.3
1,739,746 - -0.1
1,739,746 - -0.1
1,739,880 + Pf1N1B4_1507 0.22 +1.4
1,739,880 + Pf1N1B4_1507 0.22 +1.4
1,740,012 - Pf1N1B4_1507 0.40 +0.1
1,740,073 - Pf1N1B4_1507 0.49 -2.6
1,740,101 - Pf1N1B4_1507 0.53 +0.8
1,740,101 - Pf1N1B4_1507 0.53 -1.4
1,740,102 + +1.8
1,740,103 - Pf1N1B4_1507 0.53 -0.6
1,740,103 - Pf1N1B4_1507 0.53 +0.8
1,740,103 - Pf1N1B4_1507 0.53 +0.0
1,740,187 - Pf1N1B4_1507 0.64 +0.1
1,740,240 - Pf1N1B4_1507 0.71 -0.8
1,740,240 - Pf1N1B4_1507 0.71 -0.8
1,740,364 + Pf1N1B4_1507 0.88 +0.6
1,740,364 + Pf1N1B4_1507 0.88 +0.2
1,740,365 - Pf1N1B4_1507 0.88 +0.6
1,740,365 - Pf1N1B4_1507 0.88 -1.0
1,740,365 - Pf1N1B4_1507 0.88 -0.0
1,740,365 - -0.8

Or see this region's nucleotide sequence