Experiment: Cholin acetate 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1504 and Pf1N1B4_1505 overlap by 4 nucleotides Pf1N1B4_1505 and Pf1N1B4_1506 are separated by 373 nucleotides Pf1N1B4_1506 and Pf1N1B4_1507 are separated by 164 nucleotides Pf1N1B4_1507 and Pf1N1B4_1508 overlap by 4 nucleotides
Pf1N1B4_1504 - Acyl carrier protein, at 1,737,079 to 1,737,333
_1504
Pf1N1B4_1505 - FIG017861: hypothetical protein, at 1,737,330 to 1,737,875
_1505
Pf1N1B4_1506 - FIGfam138462: Acyl-CoA synthetase, AMP-(fatty) acid ligase / (3R)-hydroxymyristoyl-[ACP] dehydratase (EC 4.2.1.-), at 1,738,249 to 1,739,550
_1506
Pf1N1B4_1507 - FIG143263: Glycosyl transferase, at 1,739,715 to 1,740,449
_1507
Pf1N1B4_1508 - Lysophospholipid acyltransferase, at 1,740,446 to 1,741,393
_1508
Position (kb)
1738
1739
1740 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1737.280 kb on - strand, within Pf1N1B4_1504 at 1737.375 kb on + strand at 1737.376 kb on - strand at 1737.376 kb on - strand at 1737.376 kb on - strand at 1737.390 kb on + strand, within Pf1N1B4_1505 at 1737.390 kb on + strand, within Pf1N1B4_1505 at 1737.391 kb on - strand, within Pf1N1B4_1505 at 1737.391 kb on - strand, within Pf1N1B4_1505 at 1737.618 kb on + strand, within Pf1N1B4_1505 at 1737.618 kb on + strand, within Pf1N1B4_1505 at 1737.619 kb on - strand, within Pf1N1B4_1505 at 1737.668 kb on - strand, within Pf1N1B4_1505 at 1737.789 kb on + strand, within Pf1N1B4_1505 at 1737.789 kb on + strand, within Pf1N1B4_1505 at 1737.789 kb on + strand, within Pf1N1B4_1505 at 1737.789 kb on + strand, within Pf1N1B4_1505 at 1737.789 kb on + strand, within Pf1N1B4_1505 at 1737.789 kb on + strand, within Pf1N1B4_1505 at 1737.790 kb on - strand, within Pf1N1B4_1505 at 1737.790 kb on - strand, within Pf1N1B4_1505 at 1737.790 kb on - strand, within Pf1N1B4_1505 at 1737.807 kb on + strand, within Pf1N1B4_1505 at 1737.807 kb on + strand, within Pf1N1B4_1505 at 1737.808 kb on - strand, within Pf1N1B4_1505 at 1737.860 kb on - strand at 1738.089 kb on + strand at 1738.089 kb on + strand at 1738.089 kb on + strand at 1738.090 kb on - strand at 1738.393 kb on + strand, within Pf1N1B4_1506 at 1738.393 kb on + strand, within Pf1N1B4_1506 at 1738.393 kb on + strand, within Pf1N1B4_1506 at 1738.394 kb on - strand, within Pf1N1B4_1506 at 1738.771 kb on - strand, within Pf1N1B4_1506 at 1738.783 kb on + strand, within Pf1N1B4_1506 at 1738.783 kb on + strand, within Pf1N1B4_1506 at 1738.784 kb on - strand, within Pf1N1B4_1506 at 1738.897 kb on - strand, within Pf1N1B4_1506 at 1738.897 kb on - strand, within Pf1N1B4_1506 at 1738.988 kb on + strand, within Pf1N1B4_1506 at 1739.004 kb on - strand, within Pf1N1B4_1506 at 1739.056 kb on - strand, within Pf1N1B4_1506 at 1739.136 kb on - strand, within Pf1N1B4_1506 at 1739.282 kb on - strand, within Pf1N1B4_1506 at 1739.282 kb on - strand, within Pf1N1B4_1506 at 1739.479 kb on + strand at 1739.480 kb on - strand at 1739.480 kb on - strand at 1739.480 kb on - strand at 1739.529 kb on - strand at 1739.566 kb on - strand at 1739.624 kb on - strand at 1739.651 kb on - strand at 1739.651 kb on - strand at 1739.651 kb on - strand at 1739.721 kb on - strand at 1739.745 kb on + strand at 1739.745 kb on + strand at 1739.745 kb on + strand at 1739.745 kb on + strand at 1739.746 kb on - strand at 1739.746 kb on - strand at 1739.880 kb on + strand, within Pf1N1B4_1507 at 1739.880 kb on + strand, within Pf1N1B4_1507 at 1740.012 kb on - strand, within Pf1N1B4_1507 at 1740.073 kb on - strand, within Pf1N1B4_1507 at 1740.101 kb on - strand, within Pf1N1B4_1507 at 1740.101 kb on - strand, within Pf1N1B4_1507 at 1740.102 kb on + strand at 1740.103 kb on - strand, within Pf1N1B4_1507 at 1740.103 kb on - strand, within Pf1N1B4_1507 at 1740.103 kb on - strand, within Pf1N1B4_1507 at 1740.187 kb on - strand, within Pf1N1B4_1507 at 1740.240 kb on - strand, within Pf1N1B4_1507 at 1740.240 kb on - strand, within Pf1N1B4_1507 at 1740.364 kb on + strand, within Pf1N1B4_1507 at 1740.364 kb on + strand, within Pf1N1B4_1507 at 1740.365 kb on - strand, within Pf1N1B4_1507 at 1740.365 kb on - strand, within Pf1N1B4_1507 at 1740.365 kb on - strand, within Pf1N1B4_1507 at 1740.365 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cholin acetate 20 mM remove 1,737,280 - Pf1N1B4_1504 0.79 -2.4 1,737,375 + +1.2 1,737,376 - +0.2 1,737,376 - -0.8 1,737,376 - +0.2 1,737,390 + Pf1N1B4_1505 0.11 +1.0 1,737,390 + Pf1N1B4_1505 0.11 +0.6 1,737,391 - Pf1N1B4_1505 0.11 +1.4 1,737,391 - Pf1N1B4_1505 0.11 -1.0 1,737,618 + Pf1N1B4_1505 0.53 -0.1 1,737,618 + Pf1N1B4_1505 0.53 -0.5 1,737,619 - Pf1N1B4_1505 0.53 +0.2 1,737,668 - Pf1N1B4_1505 0.62 +0.5 1,737,789 + Pf1N1B4_1505 0.84 +0.5 1,737,789 + Pf1N1B4_1505 0.84 +0.1 1,737,789 + Pf1N1B4_1505 0.84 -0.1 1,737,789 + Pf1N1B4_1505 0.84 +0.4 1,737,789 + Pf1N1B4_1505 0.84 +1.8 1,737,789 + Pf1N1B4_1505 0.84 +0.6 1,737,790 - Pf1N1B4_1505 0.84 +0.6 1,737,790 - Pf1N1B4_1505 0.84 -0.1 1,737,790 - Pf1N1B4_1505 0.84 -2.6 1,737,807 + Pf1N1B4_1505 0.87 +0.2 1,737,807 + Pf1N1B4_1505 0.87 -0.3 1,737,808 - Pf1N1B4_1505 0.88 -0.2 1,737,860 - +1.0 1,738,089 + +1.2 1,738,089 + -1.0 1,738,089 + +1.2 1,738,090 - -1.1 1,738,393 + Pf1N1B4_1506 0.11 -1.1 1,738,393 + Pf1N1B4_1506 0.11 +0.8 1,738,393 + Pf1N1B4_1506 0.11 -1.0 1,738,394 - Pf1N1B4_1506 0.11 -1.4 1,738,771 - Pf1N1B4_1506 0.40 -1.1 1,738,783 + Pf1N1B4_1506 0.41 -0.8 1,738,783 + Pf1N1B4_1506 0.41 -1.4 1,738,784 - Pf1N1B4_1506 0.41 -0.0 1,738,897 - Pf1N1B4_1506 0.50 +0.2 1,738,897 - Pf1N1B4_1506 0.50 +1.7 1,738,988 + Pf1N1B4_1506 0.57 -0.2 1,739,004 - Pf1N1B4_1506 0.58 -0.1 1,739,056 - Pf1N1B4_1506 0.62 -0.9 1,739,136 - Pf1N1B4_1506 0.68 +0.3 1,739,282 - Pf1N1B4_1506 0.79 -1.0 1,739,282 - Pf1N1B4_1506 0.79 -1.5 1,739,479 + -0.4 1,739,480 - +0.2 1,739,480 - +0.6 1,739,480 - -0.5 1,739,529 - -0.0 1,739,566 - -1.0 1,739,624 - +0.6 1,739,651 - -2.1 1,739,651 - -0.8 1,739,651 - +0.6 1,739,721 - +0.5 1,739,745 + +1.0 1,739,745 + -1.6 1,739,745 + -3.1 1,739,745 + +0.3 1,739,746 - -0.1 1,739,746 - -0.1 1,739,880 + Pf1N1B4_1507 0.22 +1.4 1,739,880 + Pf1N1B4_1507 0.22 +1.4 1,740,012 - Pf1N1B4_1507 0.40 +0.1 1,740,073 - Pf1N1B4_1507 0.49 -2.6 1,740,101 - Pf1N1B4_1507 0.53 +0.8 1,740,101 - Pf1N1B4_1507 0.53 -1.4 1,740,102 + +1.8 1,740,103 - Pf1N1B4_1507 0.53 -0.6 1,740,103 - Pf1N1B4_1507 0.53 +0.8 1,740,103 - Pf1N1B4_1507 0.53 +0.0 1,740,187 - Pf1N1B4_1507 0.64 +0.1 1,740,240 - Pf1N1B4_1507 0.71 -0.8 1,740,240 - Pf1N1B4_1507 0.71 -0.8 1,740,364 + Pf1N1B4_1507 0.88 +0.6 1,740,364 + Pf1N1B4_1507 0.88 +0.2 1,740,365 - Pf1N1B4_1507 0.88 +0.6 1,740,365 - Pf1N1B4_1507 0.88 -1.0 1,740,365 - Pf1N1B4_1507 0.88 -0.0 1,740,365 - -0.8
Or see this region's nucleotide sequence