Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_1344

Experiment: Cholin acetate 20 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_1343 and Pf1N1B4_1344 are separated by 140 nucleotidesPf1N1B4_1344 and Pf1N1B4_1345 overlap by 1 nucleotides Pf1N1B4_1343 - Urea ABC transporter, substrate binding protein UrtA, at 1,539,345 to 1,540,610 _1343 Pf1N1B4_1344 - Urea ABC transporter, permease protein UrtB, at 1,540,751 to 1,542,250 _1344 Pf1N1B4_1345 - Urea ABC transporter, permease protein UrtC, at 1,542,250 to 1,543,329 _1345 Position (kb) 1540 1541 1542 1543Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 1539.768 kb on + strand, within Pf1N1B4_1343at 1539.768 kb on + strand, within Pf1N1B4_1343at 1539.769 kb on - strand, within Pf1N1B4_1343at 1539.769 kb on - strand, within Pf1N1B4_1343at 1539.769 kb on - strand, within Pf1N1B4_1343at 1539.850 kb on - strand, within Pf1N1B4_1343at 1539.850 kb on - strand, within Pf1N1B4_1343at 1539.850 kb on - strand, within Pf1N1B4_1343at 1539.870 kb on + strand, within Pf1N1B4_1343at 1539.870 kb on + strand, within Pf1N1B4_1343at 1539.870 kb on + strand, within Pf1N1B4_1343at 1539.870 kb on + strand, within Pf1N1B4_1343at 1539.877 kb on - strand, within Pf1N1B4_1343at 1540.071 kb on + strand, within Pf1N1B4_1343at 1540.071 kb on + strand, within Pf1N1B4_1343at 1540.072 kb on - strand, within Pf1N1B4_1343at 1540.072 kb on - strand, within Pf1N1B4_1343at 1540.197 kb on + strand, within Pf1N1B4_1343at 1540.198 kb on - strand, within Pf1N1B4_1343at 1540.198 kb on - strand, within Pf1N1B4_1343at 1540.320 kb on + strand, within Pf1N1B4_1343at 1540.320 kb on + strand, within Pf1N1B4_1343at 1540.320 kb on + strandat 1540.321 kb on - strand, within Pf1N1B4_1343at 1540.321 kb on - strand, within Pf1N1B4_1343at 1540.321 kb on - strand, within Pf1N1B4_1343at 1540.321 kb on - strand, within Pf1N1B4_1343at 1540.321 kb on - strand, within Pf1N1B4_1343at 1540.321 kb on - strand, within Pf1N1B4_1343at 1540.321 kb on - strand, within Pf1N1B4_1343at 1540.321 kb on - strand, within Pf1N1B4_1343at 1540.321 kb on - strand, within Pf1N1B4_1343at 1540.321 kb on - strand, within Pf1N1B4_1343at 1540.321 kb on - strand, within Pf1N1B4_1343at 1540.334 kb on + strand, within Pf1N1B4_1343at 1540.334 kb on + strand, within Pf1N1B4_1343at 1540.334 kb on + strand, within Pf1N1B4_1343at 1540.334 kb on + strand, within Pf1N1B4_1343at 1540.334 kb on + strand, within Pf1N1B4_1343at 1540.334 kb on + strand, within Pf1N1B4_1343at 1540.334 kb on + strand, within Pf1N1B4_1343at 1540.335 kb on - strand, within Pf1N1B4_1343at 1540.335 kb on - strand, within Pf1N1B4_1343at 1540.335 kb on - strand, within Pf1N1B4_1343at 1540.335 kb on - strand, within Pf1N1B4_1343at 1540.335 kb on - strand, within Pf1N1B4_1343at 1540.335 kb on - strand, within Pf1N1B4_1343at 1540.335 kb on - strand, within Pf1N1B4_1343at 1541.380 kb on + strand, within Pf1N1B4_1344at 1541.381 kb on - strand, within Pf1N1B4_1344at 1541.381 kb on - strand, within Pf1N1B4_1344at 1541.381 kb on - strand, within Pf1N1B4_1344at 1541.381 kb on - strand, within Pf1N1B4_1344at 1541.404 kb on - strand, within Pf1N1B4_1344at 1541.498 kb on + strand, within Pf1N1B4_1344at 1541.499 kb on - strand, within Pf1N1B4_1344at 1541.499 kb on - strand, within Pf1N1B4_1344at 1542.035 kb on + strand, within Pf1N1B4_1344at 1542.035 kb on + strand, within Pf1N1B4_1344at 1542.036 kb on - strand, within Pf1N1B4_1344at 1542.036 kb on - strand, within Pf1N1B4_1344at 1542.036 kb on - strand, within Pf1N1B4_1344at 1542.036 kb on - strand, within Pf1N1B4_1344at 1542.036 kb on - strand, within Pf1N1B4_1344at 1542.333 kb on - strandat 1542.333 kb on - strandat 1542.333 kb on - strandat 1542.333 kb on - strandat 1542.395 kb on - strand, within Pf1N1B4_1345at 1542.532 kb on + strand, within Pf1N1B4_1345at 1542.532 kb on + strand, within Pf1N1B4_1345at 1542.532 kb on + strand, within Pf1N1B4_1345at 1542.533 kb on - strand, within Pf1N1B4_1345at 1542.533 kb on - strand, within Pf1N1B4_1345at 1542.533 kb on - strand, within Pf1N1B4_1345at 1542.533 kb on - strand, within Pf1N1B4_1345at 1542.721 kb on + strand, within Pf1N1B4_1345at 1542.721 kb on + strand, within Pf1N1B4_1345at 1542.722 kb on - strand, within Pf1N1B4_1345at 1542.722 kb on - strand, within Pf1N1B4_1345at 1543.066 kb on + strandat 1543.066 kb on + strand, within Pf1N1B4_1345at 1543.066 kb on + strand, within Pf1N1B4_1345at 1543.067 kb on - strand, within Pf1N1B4_1345at 1543.067 kb on - strand, within Pf1N1B4_1345at 1543.087 kb on - strand, within Pf1N1B4_1345at 1543.234 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Cholin acetate 20 mM
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1,539,768 + Pf1N1B4_1343 0.33 +0.4
1,539,768 + Pf1N1B4_1343 0.33 +0.4
1,539,769 - Pf1N1B4_1343 0.33 +0.1
1,539,769 - Pf1N1B4_1343 0.33 -0.4
1,539,769 - Pf1N1B4_1343 0.33 -0.1
1,539,850 - Pf1N1B4_1343 0.40 +0.4
1,539,850 - Pf1N1B4_1343 0.40 -0.9
1,539,850 - Pf1N1B4_1343 0.40 +1.9
1,539,870 + Pf1N1B4_1343 0.41 -0.4
1,539,870 + Pf1N1B4_1343 0.41 +0.6
1,539,870 + Pf1N1B4_1343 0.41 -0.1
1,539,870 + Pf1N1B4_1343 0.41 +1.9
1,539,877 - Pf1N1B4_1343 0.42 +0.3
1,540,071 + Pf1N1B4_1343 0.57 +1.3
1,540,071 + Pf1N1B4_1343 0.57 +0.5
1,540,072 - Pf1N1B4_1343 0.57 -0.9
1,540,072 - Pf1N1B4_1343 0.57 +0.6
1,540,197 + Pf1N1B4_1343 0.67 -1.2
1,540,198 - Pf1N1B4_1343 0.67 -0.0
1,540,198 - Pf1N1B4_1343 0.67 +0.3
1,540,320 + Pf1N1B4_1343 0.77 -1.0
1,540,320 + Pf1N1B4_1343 0.77 +0.5
1,540,320 + +2.1
1,540,321 - Pf1N1B4_1343 0.77 -0.4
1,540,321 - Pf1N1B4_1343 0.77 +0.9
1,540,321 - Pf1N1B4_1343 0.77 -0.2
1,540,321 - Pf1N1B4_1343 0.77 +0.7
1,540,321 - Pf1N1B4_1343 0.77 +0.6
1,540,321 - Pf1N1B4_1343 0.77 -1.4
1,540,321 - Pf1N1B4_1343 0.77 +1.7
1,540,321 - Pf1N1B4_1343 0.77 -0.7
1,540,321 - Pf1N1B4_1343 0.77 +1.1
1,540,321 - Pf1N1B4_1343 0.77 -0.1
1,540,321 - Pf1N1B4_1343 0.77 +0.9
1,540,334 + Pf1N1B4_1343 0.78 -1.1
1,540,334 + Pf1N1B4_1343 0.78 +0.3
1,540,334 + Pf1N1B4_1343 0.78 +1.0
1,540,334 + Pf1N1B4_1343 0.78 +0.4
1,540,334 + Pf1N1B4_1343 0.78 -1.4
1,540,334 + Pf1N1B4_1343 0.78 +0.9
1,540,334 + Pf1N1B4_1343 0.78 -0.9
1,540,335 - Pf1N1B4_1343 0.78 +0.5
1,540,335 - Pf1N1B4_1343 0.78 -0.4
1,540,335 - Pf1N1B4_1343 0.78 -0.2
1,540,335 - Pf1N1B4_1343 0.78 +0.8
1,540,335 - Pf1N1B4_1343 0.78 -2.0
1,540,335 - Pf1N1B4_1343 0.78 +0.1
1,540,335 - Pf1N1B4_1343 0.78 -0.7
1,541,380 + Pf1N1B4_1344 0.42 -1.4
1,541,381 - Pf1N1B4_1344 0.42 -1.1
1,541,381 - Pf1N1B4_1344 0.42 -1.2
1,541,381 - Pf1N1B4_1344 0.42 -0.4
1,541,381 - Pf1N1B4_1344 0.42 -0.2
1,541,404 - Pf1N1B4_1344 0.44 +0.6
1,541,498 + Pf1N1B4_1344 0.50 -1.7
1,541,499 - Pf1N1B4_1344 0.50 -0.4
1,541,499 - Pf1N1B4_1344 0.50 -2.9
1,542,035 + Pf1N1B4_1344 0.86 +1.1
1,542,035 + Pf1N1B4_1344 0.86 -0.7
1,542,036 - Pf1N1B4_1344 0.86 +0.1
1,542,036 - Pf1N1B4_1344 0.86 -1.7
1,542,036 - Pf1N1B4_1344 0.86 -0.5
1,542,036 - Pf1N1B4_1344 0.86 -0.1
1,542,036 - Pf1N1B4_1344 0.86 -0.9
1,542,333 - +1.1
1,542,333 - -0.4
1,542,333 - +0.1
1,542,333 - -0.4
1,542,395 - Pf1N1B4_1345 0.13 -0.4
1,542,532 + Pf1N1B4_1345 0.26 +0.1
1,542,532 + Pf1N1B4_1345 0.26 +0.1
1,542,532 + Pf1N1B4_1345 0.26 -1.2
1,542,533 - Pf1N1B4_1345 0.26 -0.2
1,542,533 - Pf1N1B4_1345 0.26 -0.7
1,542,533 - Pf1N1B4_1345 0.26 +0.7
1,542,533 - Pf1N1B4_1345 0.26 +1.1
1,542,721 + Pf1N1B4_1345 0.44 +0.9
1,542,721 + Pf1N1B4_1345 0.44 +1.7
1,542,722 - Pf1N1B4_1345 0.44 +0.7
1,542,722 - Pf1N1B4_1345 0.44 +1.1
1,543,066 + -0.4
1,543,066 + Pf1N1B4_1345 0.76 -0.9
1,543,066 + Pf1N1B4_1345 0.76 +0.0
1,543,067 - Pf1N1B4_1345 0.76 +1.1
1,543,067 - Pf1N1B4_1345 0.76 -2.0
1,543,087 - Pf1N1B4_1345 0.78 +0.3
1,543,234 + -0.9

Or see this region's nucleotide sequence