Experiment: Cholin acetate 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1208 and Pf1N1B4_1209 are separated by 132 nucleotides Pf1N1B4_1209 and Pf1N1B4_1210 overlap by 11 nucleotides Pf1N1B4_1210 and Pf1N1B4_1211 are separated by 234 nucleotides
Pf1N1B4_1208 - Modulator of drug activity B, at 1,371,670 to 1,372,260
_1208
Pf1N1B4_1209 - FIG00959330: hypothetical protein, at 1,372,393 to 1,372,737
_1209
Pf1N1B4_1210 - membrane protein, putative, at 1,372,727 to 1,373,491
_1210
Pf1N1B4_1211 - Hydrogen peroxide-inducible genes activator, at 1,373,726 to 1,374,658
_1211
Position (kb)
1372
1373
1374 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1371.748 kb on + strand, within Pf1N1B4_1208 at 1371.748 kb on + strand, within Pf1N1B4_1208 at 1371.749 kb on - strand, within Pf1N1B4_1208 at 1371.749 kb on - strand, within Pf1N1B4_1208 at 1371.795 kb on + strand, within Pf1N1B4_1208 at 1371.796 kb on - strand, within Pf1N1B4_1208 at 1371.818 kb on + strand, within Pf1N1B4_1208 at 1371.818 kb on + strand, within Pf1N1B4_1208 at 1371.818 kb on + strand, within Pf1N1B4_1208 at 1371.818 kb on + strand, within Pf1N1B4_1208 at 1371.818 kb on + strand, within Pf1N1B4_1208 at 1371.818 kb on + strand, within Pf1N1B4_1208 at 1371.818 kb on + strand, within Pf1N1B4_1208 at 1371.819 kb on - strand, within Pf1N1B4_1208 at 1371.819 kb on - strand, within Pf1N1B4_1208 at 1371.903 kb on + strand, within Pf1N1B4_1208 at 1371.903 kb on + strand, within Pf1N1B4_1208 at 1371.903 kb on + strand, within Pf1N1B4_1208 at 1371.903 kb on + strand, within Pf1N1B4_1208 at 1371.904 kb on - strand, within Pf1N1B4_1208 at 1371.904 kb on - strand, within Pf1N1B4_1208 at 1371.904 kb on - strand, within Pf1N1B4_1208 at 1371.904 kb on - strand, within Pf1N1B4_1208 at 1371.904 kb on - strand, within Pf1N1B4_1208 at 1371.904 kb on - strand, within Pf1N1B4_1208 at 1371.904 kb on - strand, within Pf1N1B4_1208 at 1371.904 kb on - strand, within Pf1N1B4_1208 at 1371.904 kb on - strand, within Pf1N1B4_1208 at 1371.905 kb on + strand, within Pf1N1B4_1208 at 1371.905 kb on + strand, within Pf1N1B4_1208 at 1371.905 kb on + strand, within Pf1N1B4_1208 at 1371.905 kb on + strand, within Pf1N1B4_1208 at 1371.906 kb on - strand, within Pf1N1B4_1208 at 1371.906 kb on - strand, within Pf1N1B4_1208 at 1371.906 kb on - strand, within Pf1N1B4_1208 at 1371.938 kb on + strand, within Pf1N1B4_1208 at 1371.939 kb on - strand, within Pf1N1B4_1208 at 1372.019 kb on + strand, within Pf1N1B4_1208 at 1372.019 kb on + strand, within Pf1N1B4_1208 at 1372.020 kb on - strand, within Pf1N1B4_1208 at 1372.067 kb on + strand, within Pf1N1B4_1208 at 1372.067 kb on + strand, within Pf1N1B4_1208 at 1372.068 kb on - strand, within Pf1N1B4_1208 at 1372.068 kb on - strand, within Pf1N1B4_1208 at 1372.068 kb on - strand, within Pf1N1B4_1208 at 1372.144 kb on + strand, within Pf1N1B4_1208 at 1372.144 kb on + strand, within Pf1N1B4_1208 at 1372.144 kb on + strand, within Pf1N1B4_1208 at 1372.145 kb on - strand, within Pf1N1B4_1208 at 1372.145 kb on - strand, within Pf1N1B4_1208 at 1372.145 kb on - strand, within Pf1N1B4_1208 at 1372.323 kb on + strand at 1372.323 kb on + strand at 1372.323 kb on + strand at 1372.323 kb on + strand at 1372.323 kb on + strand at 1372.323 kb on + strand at 1372.323 kb on + strand at 1372.324 kb on - strand at 1372.324 kb on - strand at 1372.324 kb on - strand at 1372.504 kb on + strand, within Pf1N1B4_1209 at 1372.504 kb on + strand, within Pf1N1B4_1209 at 1372.504 kb on + strand, within Pf1N1B4_1209 at 1372.504 kb on + strand, within Pf1N1B4_1209 at 1372.504 kb on + strand, within Pf1N1B4_1209 at 1372.504 kb on + strand, within Pf1N1B4_1209 at 1372.504 kb on + strand, within Pf1N1B4_1209 at 1372.504 kb on + strand, within Pf1N1B4_1209 at 1372.504 kb on + strand, within Pf1N1B4_1209 at 1372.504 kb on + strand, within Pf1N1B4_1209 at 1372.505 kb on - strand, within Pf1N1B4_1209 at 1372.505 kb on - strand, within Pf1N1B4_1209 at 1372.505 kb on - strand, within Pf1N1B4_1209 at 1372.505 kb on - strand, within Pf1N1B4_1209 at 1372.581 kb on + strand, within Pf1N1B4_1209 at 1372.581 kb on + strand, within Pf1N1B4_1209 at 1372.582 kb on - strand, within Pf1N1B4_1209 at 1372.588 kb on + strand at 1372.588 kb on + strand, within Pf1N1B4_1209 at 1372.589 kb on - strand, within Pf1N1B4_1209 at 1372.620 kb on + strand, within Pf1N1B4_1209 at 1372.759 kb on + strand at 1372.759 kb on + strand at 1372.759 kb on + strand at 1372.759 kb on + strand at 1372.759 kb on + strand at 1372.759 kb on + strand at 1372.760 kb on - strand at 1372.778 kb on + strand at 1372.779 kb on - strand at 1372.998 kb on + strand, within Pf1N1B4_1210 at 1372.998 kb on + strand, within Pf1N1B4_1210 at 1372.999 kb on - strand, within Pf1N1B4_1210 at 1372.999 kb on - strand, within Pf1N1B4_1210 at 1372.999 kb on - strand, within Pf1N1B4_1210 at 1373.133 kb on + strand, within Pf1N1B4_1210 at 1373.133 kb on + strand, within Pf1N1B4_1210 at 1373.134 kb on - strand, within Pf1N1B4_1210 at 1373.134 kb on - strand, within Pf1N1B4_1210 at 1373.134 kb on - strand, within Pf1N1B4_1210 at 1373.134 kb on - strand, within Pf1N1B4_1210 at 1373.134 kb on - strand, within Pf1N1B4_1210 at 1373.134 kb on - strand, within Pf1N1B4_1210 at 1373.134 kb on - strand at 1373.134 kb on - strand, within Pf1N1B4_1210 at 1373.134 kb on - strand, within Pf1N1B4_1210 at 1373.135 kb on + strand, within Pf1N1B4_1210 at 1373.136 kb on - strand, within Pf1N1B4_1210 at 1373.441 kb on + strand at 1373.469 kb on + strand at 1373.470 kb on - strand at 1373.485 kb on + strand at 1373.486 kb on - strand at 1373.486 kb on - strand at 1373.498 kb on - strand at 1373.534 kb on + strand at 1373.534 kb on + strand at 1373.534 kb on + strand at 1373.535 kb on - strand at 1373.536 kb on + strand at 1373.536 kb on + strand at 1373.536 kb on + strand at 1373.756 kb on + strand at 1373.757 kb on - strand at 1373.757 kb on - strand at 1374.026 kb on - strand, within Pf1N1B4_1211 at 1374.026 kb on - strand, within Pf1N1B4_1211 at 1374.045 kb on - strand, within Pf1N1B4_1211 at 1374.045 kb on - strand, within Pf1N1B4_1211 at 1374.273 kb on + strand, within Pf1N1B4_1211 at 1374.273 kb on + strand, within Pf1N1B4_1211 at 1374.302 kb on + strand, within Pf1N1B4_1211 at 1374.302 kb on + strand, within Pf1N1B4_1211 at 1374.303 kb on - strand at 1374.303 kb on - strand, within Pf1N1B4_1211 at 1374.303 kb on - strand, within Pf1N1B4_1211 at 1374.303 kb on - strand, within Pf1N1B4_1211 at 1374.303 kb on - strand, within Pf1N1B4_1211 at 1374.303 kb on - strand, within Pf1N1B4_1211 at 1374.303 kb on - strand, within Pf1N1B4_1211 at 1374.303 kb on - strand, within Pf1N1B4_1211 at 1374.303 kb on - strand at 1374.303 kb on - strand, within Pf1N1B4_1211 at 1374.338 kb on + strand, within Pf1N1B4_1211 at 1374.338 kb on + strand, within Pf1N1B4_1211
Per-strain Table
Position Strand Gene LocusTag Fraction Cholin acetate 20 mM remove 1,371,748 + Pf1N1B4_1208 0.13 -0.5 1,371,748 + Pf1N1B4_1208 0.13 +0.1 1,371,749 - Pf1N1B4_1208 0.13 -1.2 1,371,749 - Pf1N1B4_1208 0.13 -2.6 1,371,795 + Pf1N1B4_1208 0.21 -2.3 1,371,796 - Pf1N1B4_1208 0.21 -0.1 1,371,818 + Pf1N1B4_1208 0.25 +1.1 1,371,818 + Pf1N1B4_1208 0.25 -0.5 1,371,818 + Pf1N1B4_1208 0.25 +0.6 1,371,818 + Pf1N1B4_1208 0.25 +0.8 1,371,818 + Pf1N1B4_1208 0.25 +0.0 1,371,818 + Pf1N1B4_1208 0.25 -0.9 1,371,818 + Pf1N1B4_1208 0.25 -0.9 1,371,819 - Pf1N1B4_1208 0.25 -1.2 1,371,819 - Pf1N1B4_1208 0.25 +1.8 1,371,903 + Pf1N1B4_1208 0.39 +0.6 1,371,903 + Pf1N1B4_1208 0.39 +0.3 1,371,903 + Pf1N1B4_1208 0.39 +1.7 1,371,903 + Pf1N1B4_1208 0.39 -1.2 1,371,904 - Pf1N1B4_1208 0.40 -0.0 1,371,904 - Pf1N1B4_1208 0.40 +0.7 1,371,904 - Pf1N1B4_1208 0.40 -0.5 1,371,904 - Pf1N1B4_1208 0.40 -1.1 1,371,904 - Pf1N1B4_1208 0.40 +3.1 1,371,904 - Pf1N1B4_1208 0.40 +0.0 1,371,904 - Pf1N1B4_1208 0.40 +1.7 1,371,904 - Pf1N1B4_1208 0.40 -0.1 1,371,904 - Pf1N1B4_1208 0.40 -1.2 1,371,905 + Pf1N1B4_1208 0.40 -0.5 1,371,905 + Pf1N1B4_1208 0.40 +0.1 1,371,905 + Pf1N1B4_1208 0.40 -0.2 1,371,905 + Pf1N1B4_1208 0.40 -0.8 1,371,906 - Pf1N1B4_1208 0.40 +1.8 1,371,906 - Pf1N1B4_1208 0.40 -1.2 1,371,906 - Pf1N1B4_1208 0.40 +1.7 1,371,938 + Pf1N1B4_1208 0.45 -1.2 1,371,939 - Pf1N1B4_1208 0.46 +0.7 1,372,019 + Pf1N1B4_1208 0.59 -0.8 1,372,019 + Pf1N1B4_1208 0.59 -1.2 1,372,020 - Pf1N1B4_1208 0.59 -2.1 1,372,067 + Pf1N1B4_1208 0.67 -3.1 1,372,067 + Pf1N1B4_1208 0.67 +0.4 1,372,068 - Pf1N1B4_1208 0.67 +0.2 1,372,068 - Pf1N1B4_1208 0.67 +0.4 1,372,068 - Pf1N1B4_1208 0.67 +0.7 1,372,144 + Pf1N1B4_1208 0.80 -1.9 1,372,144 + Pf1N1B4_1208 0.80 -1.7 1,372,144 + Pf1N1B4_1208 0.80 +0.2 1,372,145 - Pf1N1B4_1208 0.80 +0.1 1,372,145 - Pf1N1B4_1208 0.80 -0.0 1,372,145 - Pf1N1B4_1208 0.80 -0.7 1,372,323 + +1.7 1,372,323 + +0.8 1,372,323 + +0.1 1,372,323 + -0.8 1,372,323 + +0.9 1,372,323 + +1.1 1,372,323 + +0.1 1,372,324 - +0.1 1,372,324 - +1.1 1,372,324 - -0.7 1,372,504 + Pf1N1B4_1209 0.32 +1.0 1,372,504 + Pf1N1B4_1209 0.32 +1.1 1,372,504 + Pf1N1B4_1209 0.32 -1.3 1,372,504 + Pf1N1B4_1209 0.32 -0.2 1,372,504 + Pf1N1B4_1209 0.32 +0.6 1,372,504 + Pf1N1B4_1209 0.32 +0.7 1,372,504 + Pf1N1B4_1209 0.32 +0.7 1,372,504 + Pf1N1B4_1209 0.32 -0.1 1,372,504 + Pf1N1B4_1209 0.32 +0.9 1,372,504 + Pf1N1B4_1209 0.32 -1.7 1,372,505 - Pf1N1B4_1209 0.32 -1.7 1,372,505 - Pf1N1B4_1209 0.32 +0.1 1,372,505 - Pf1N1B4_1209 0.32 -1.7 1,372,505 - Pf1N1B4_1209 0.32 -0.9 1,372,581 + Pf1N1B4_1209 0.54 +0.6 1,372,581 + Pf1N1B4_1209 0.54 -0.4 1,372,582 - Pf1N1B4_1209 0.55 +0.8 1,372,588 + +1.1 1,372,588 + Pf1N1B4_1209 0.57 -0.5 1,372,589 - Pf1N1B4_1209 0.57 +0.5 1,372,620 + Pf1N1B4_1209 0.66 +0.5 1,372,759 + +2.1 1,372,759 + -0.6 1,372,759 + -0.1 1,372,759 + -0.8 1,372,759 + -0.2 1,372,759 + +0.4 1,372,760 - +0.1 1,372,778 + +0.4 1,372,779 - +2.1 1,372,998 + Pf1N1B4_1210 0.35 +0.7 1,372,998 + Pf1N1B4_1210 0.35 -0.9 1,372,999 - Pf1N1B4_1210 0.36 -1.9 1,372,999 - Pf1N1B4_1210 0.36 +0.1 1,372,999 - Pf1N1B4_1210 0.36 -1.5 1,373,133 + Pf1N1B4_1210 0.53 -2.2 1,373,133 + Pf1N1B4_1210 0.53 -0.9 1,373,134 - Pf1N1B4_1210 0.53 -0.5 1,373,134 - Pf1N1B4_1210 0.53 -1.2 1,373,134 - Pf1N1B4_1210 0.53 -0.6 1,373,134 - Pf1N1B4_1210 0.53 -0.5 1,373,134 - Pf1N1B4_1210 0.53 -0.9 1,373,134 - Pf1N1B4_1210 0.53 +0.3 1,373,134 - -0.9 1,373,134 - Pf1N1B4_1210 0.53 -1.9 1,373,134 - Pf1N1B4_1210 0.53 -2.1 1,373,135 + Pf1N1B4_1210 0.53 +0.1 1,373,136 - Pf1N1B4_1210 0.53 +0.9 1,373,441 + -1.5 1,373,469 + -0.0 1,373,470 - -2.2 1,373,485 + -0.1 1,373,486 - +0.5 1,373,486 - +0.8 1,373,498 - +0.4 1,373,534 + -0.5 1,373,534 + -0.0 1,373,534 + +1.1 1,373,535 - +0.1 1,373,536 + -0.2 1,373,536 + -0.9 1,373,536 + -0.2 1,373,756 + -0.4 1,373,757 - -0.5 1,373,757 - +0.3 1,374,026 - Pf1N1B4_1211 0.32 +0.4 1,374,026 - Pf1N1B4_1211 0.32 +0.4 1,374,045 - Pf1N1B4_1211 0.34 +0.2 1,374,045 - Pf1N1B4_1211 0.34 +0.1 1,374,273 + Pf1N1B4_1211 0.59 -0.7 1,374,273 + Pf1N1B4_1211 0.59 -0.9 1,374,302 + Pf1N1B4_1211 0.62 +0.1 1,374,302 + Pf1N1B4_1211 0.62 +0.6 1,374,303 - +1.1 1,374,303 - Pf1N1B4_1211 0.62 +0.1 1,374,303 - Pf1N1B4_1211 0.62 -0.6 1,374,303 - Pf1N1B4_1211 0.62 -0.1 1,374,303 - Pf1N1B4_1211 0.62 -0.8 1,374,303 - Pf1N1B4_1211 0.62 +0.2 1,374,303 - Pf1N1B4_1211 0.62 -0.7 1,374,303 - Pf1N1B4_1211 0.62 -0.2 1,374,303 - +1.1 1,374,303 - Pf1N1B4_1211 0.62 -1.2 1,374,338 + Pf1N1B4_1211 0.66 -0.2 1,374,338 + Pf1N1B4_1211 0.66 -1.2
Or see this region's nucleotide sequence