Experiment: Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS08655 and MMP_RS08660 are separated by 49 nucleotides MMP_RS08660 and MMP_RS08665 are separated by 132 nucleotides MMP_RS08665 and MMP_RS08670 are separated by 0 nucleotides
MMP_RS08655: MMP_RS08655 - phosphoadenosine phosphosulfate reductase family protein, at 1,622,934 to 1,624,220
_RS08655
MMP_RS08660: MMP_RS08660 - DHH family phosphoesterase, at 1,624,270 to 1,625,673
_RS08660
MMP_RS08665: MMP_RS08665 - hypothetical protein, at 1,625,806 to 1,626,447
_RS08665
MMP_RS08670: MMP_RS08670 - metallophosphoesterase, at 1,626,448 to 1,627,131
_RS08670
Position (kb)
1624
1625
1626 Strain fitness (log2 ratio)
-2
-1
0
1 at 1623.495 kb on - strand, within MMP_RS08655 at 1623.603 kb on + strand, within MMP_RS08655 at 1623.752 kb on - strand, within MMP_RS08655 at 1623.752 kb on - strand, within MMP_RS08655 at 1623.753 kb on + strand, within MMP_RS08655 at 1623.753 kb on + strand, within MMP_RS08655 at 1623.753 kb on + strand, within MMP_RS08655 at 1623.753 kb on + strand, within MMP_RS08655 at 1623.753 kb on + strand, within MMP_RS08655 at 1624.002 kb on + strand, within MMP_RS08655 at 1624.003 kb on - strand, within MMP_RS08655 at 1624.004 kb on + strand, within MMP_RS08655 at 1624.004 kb on + strand, within MMP_RS08655 at 1624.004 kb on + strand, within MMP_RS08655 at 1624.170 kb on - strand at 1624.171 kb on + strand at 1624.180 kb on + strand at 1624.180 kb on + strand at 1624.221 kb on + strand at 1624.251 kb on + strand at 1624.251 kb on + strand at 1624.290 kb on + strand at 1624.320 kb on + strand at 1624.570 kb on + strand, within MMP_RS08660 at 1624.570 kb on + strand, within MMP_RS08660 at 1624.753 kb on + strand, within MMP_RS08660 at 1624.846 kb on - strand, within MMP_RS08660 at 1624.867 kb on + strand, within MMP_RS08660 at 1624.869 kb on + strand, within MMP_RS08660 at 1624.937 kb on - strand, within MMP_RS08660 at 1624.984 kb on - strand, within MMP_RS08660 at 1624.986 kb on - strand, within MMP_RS08660 at 1624.987 kb on + strand, within MMP_RS08660 at 1624.993 kb on + strand, within MMP_RS08660 at 1625.042 kb on - strand, within MMP_RS08660 at 1625.049 kb on - strand, within MMP_RS08660 at 1625.050 kb on + strand, within MMP_RS08660 at 1625.070 kb on - strand, within MMP_RS08660 at 1625.070 kb on - strand, within MMP_RS08660 at 1625.140 kb on - strand, within MMP_RS08660 at 1625.326 kb on - strand, within MMP_RS08660 at 1625.327 kb on + strand, within MMP_RS08660 at 1625.453 kb on + strand, within MMP_RS08660 at 1625.459 kb on + strand, within MMP_RS08660 at 1625.467 kb on + strand, within MMP_RS08660 at 1625.533 kb on + strand at 1625.575 kb on - strand at 1625.608 kb on - strand at 1625.632 kb on + strand at 1625.699 kb on - strand at 1625.746 kb on + strand at 1625.747 kb on - strand at 1625.748 kb on + strand at 1625.762 kb on + strand at 1625.763 kb on - strand at 1625.764 kb on + strand at 1625.764 kb on + strand at 1625.765 kb on - strand at 1625.765 kb on - strand at 1625.766 kb on + strand at 1625.802 kb on + strand at 1625.806 kb on + strand at 1625.846 kb on - strand at 1625.849 kb on - strand at 1625.849 kb on - strand at 1625.850 kb on + strand at 1625.850 kb on + strand at 1625.858 kb on - strand at 1625.890 kb on - strand, within MMP_RS08665 at 1625.917 kb on + strand, within MMP_RS08665 at 1625.922 kb on - strand, within MMP_RS08665 at 1625.925 kb on - strand, within MMP_RS08665 at 1625.925 kb on - strand, within MMP_RS08665 at 1625.929 kb on - strand, within MMP_RS08665 at 1625.929 kb on - strand, within MMP_RS08665 at 1625.929 kb on - strand, within MMP_RS08665 at 1625.929 kb on - strand, within MMP_RS08665 at 1625.930 kb on + strand, within MMP_RS08665 at 1625.930 kb on + strand, within MMP_RS08665 at 1625.930 kb on + strand, within MMP_RS08665 at 1625.933 kb on - strand, within MMP_RS08665 at 1625.934 kb on + strand, within MMP_RS08665 at 1625.976 kb on - strand, within MMP_RS08665 at 1626.000 kb on + strand, within MMP_RS08665 at 1626.050 kb on - strand, within MMP_RS08665 at 1626.050 kb on - strand, within MMP_RS08665 at 1626.051 kb on + strand, within MMP_RS08665 at 1626.054 kb on - strand, within MMP_RS08665 at 1626.103 kb on - strand, within MMP_RS08665 at 1626.104 kb on + strand, within MMP_RS08665 at 1626.106 kb on + strand, within MMP_RS08665 at 1626.106 kb on + strand, within MMP_RS08665 at 1626.120 kb on + strand, within MMP_RS08665 at 1626.150 kb on + strand, within MMP_RS08665 at 1626.152 kb on - strand, within MMP_RS08665 at 1626.152 kb on - strand, within MMP_RS08665 at 1626.155 kb on + strand, within MMP_RS08665 at 1626.155 kb on + strand, within MMP_RS08665 at 1626.155 kb on + strand, within MMP_RS08665 at 1626.156 kb on - strand, within MMP_RS08665 at 1626.157 kb on + strand, within MMP_RS08665 at 1626.189 kb on - strand, within MMP_RS08665 at 1626.211 kb on + strand, within MMP_RS08665 at 1626.306 kb on + strand, within MMP_RS08665 at 1626.314 kb on - strand, within MMP_RS08665 at 1626.314 kb on - strand, within MMP_RS08665 at 1626.314 kb on - strand, within MMP_RS08665 at 1626.342 kb on + strand, within MMP_RS08665 at 1626.365 kb on - strand, within MMP_RS08665 at 1626.483 kb on - strand at 1626.488 kb on - strand at 1626.489 kb on + strand at 1626.489 kb on + strand at 1626.490 kb on - strand at 1626.491 kb on + strand at 1626.492 kb on - strand at 1626.518 kb on + strand, within MMP_RS08670 at 1626.518 kb on + strand, within MMP_RS08670 at 1626.529 kb on + strand, within MMP_RS08670 at 1626.546 kb on - strand, within MMP_RS08670 at 1626.592 kb on + strand, within MMP_RS08670 at 1626.661 kb on + strand, within MMP_RS08670
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE remove 1,623,495 - MMP_RS08655 0.44 -0.2 1,623,603 + MMP_RS08655 0.52 +0.0 1,623,752 - MMP_RS08655 0.64 +0.1 1,623,752 - MMP_RS08655 0.64 -0.2 1,623,753 + MMP_RS08655 0.64 +0.2 1,623,753 + MMP_RS08655 0.64 -0.4 1,623,753 + MMP_RS08655 0.64 +0.2 1,623,753 + MMP_RS08655 0.64 -0.2 1,623,753 + MMP_RS08655 0.64 +0.0 1,624,002 + MMP_RS08655 0.83 -0.5 1,624,003 - MMP_RS08655 0.83 -1.1 1,624,004 + MMP_RS08655 0.83 -0.0 1,624,004 + MMP_RS08655 0.83 -0.1 1,624,004 + MMP_RS08655 0.83 -0.3 1,624,170 - -0.3 1,624,171 + +0.1 1,624,180 + +0.0 1,624,180 + +0.2 1,624,221 + -0.4 1,624,251 + +0.3 1,624,251 + +0.0 1,624,290 + -0.1 1,624,320 + -1.0 1,624,570 + MMP_RS08660 0.21 -1.3 1,624,570 + MMP_RS08660 0.21 -1.0 1,624,753 + MMP_RS08660 0.34 -0.8 1,624,846 - MMP_RS08660 0.41 +0.1 1,624,867 + MMP_RS08660 0.43 -0.4 1,624,869 + MMP_RS08660 0.43 -1.8 1,624,937 - MMP_RS08660 0.48 -1.3 1,624,984 - MMP_RS08660 0.51 -1.2 1,624,986 - MMP_RS08660 0.51 -1.7 1,624,987 + MMP_RS08660 0.51 -1.2 1,624,993 + MMP_RS08660 0.51 +0.0 1,625,042 - MMP_RS08660 0.55 -0.9 1,625,049 - MMP_RS08660 0.55 -1.8 1,625,050 + MMP_RS08660 0.56 -0.7 1,625,070 - MMP_RS08660 0.57 -0.9 1,625,070 - MMP_RS08660 0.57 +0.6 1,625,140 - MMP_RS08660 0.62 -0.9 1,625,326 - MMP_RS08660 0.75 -0.1 1,625,327 + MMP_RS08660 0.75 +0.4 1,625,453 + MMP_RS08660 0.84 -2.0 1,625,459 + MMP_RS08660 0.85 -1.7 1,625,467 + MMP_RS08660 0.85 -1.6 1,625,533 + +0.0 1,625,575 - -1.3 1,625,608 - -1.1 1,625,632 + -1.1 1,625,699 - +0.4 1,625,746 + +0.1 1,625,747 - +0.1 1,625,748 + +0.4 1,625,762 + +0.6 1,625,763 - -0.3 1,625,764 + +0.4 1,625,764 + -0.4 1,625,765 - +0.3 1,625,765 - -0.3 1,625,766 + +0.1 1,625,802 + +0.1 1,625,806 + +0.6 1,625,846 - -0.2 1,625,849 - -0.0 1,625,849 - -0.2 1,625,850 + +0.2 1,625,850 + +0.3 1,625,858 - -0.0 1,625,890 - MMP_RS08665 0.13 -0.4 1,625,917 + MMP_RS08665 0.17 -0.3 1,625,922 - MMP_RS08665 0.18 +0.7 1,625,925 - MMP_RS08665 0.19 +0.0 1,625,925 - MMP_RS08665 0.19 +0.3 1,625,929 - MMP_RS08665 0.19 +0.1 1,625,929 - MMP_RS08665 0.19 +0.2 1,625,929 - MMP_RS08665 0.19 -0.2 1,625,929 - MMP_RS08665 0.19 +0.0 1,625,930 + MMP_RS08665 0.19 -0.2 1,625,930 + MMP_RS08665 0.19 +0.3 1,625,930 + MMP_RS08665 0.19 -0.4 1,625,933 - MMP_RS08665 0.20 +0.2 1,625,934 + MMP_RS08665 0.20 +0.3 1,625,976 - MMP_RS08665 0.26 +0.0 1,626,000 + MMP_RS08665 0.30 +0.6 1,626,050 - MMP_RS08665 0.38 +0.1 1,626,050 - MMP_RS08665 0.38 +0.4 1,626,051 + MMP_RS08665 0.38 -0.1 1,626,054 - MMP_RS08665 0.39 +0.4 1,626,103 - MMP_RS08665 0.46 -0.1 1,626,104 + MMP_RS08665 0.46 -0.2 1,626,106 + MMP_RS08665 0.47 +0.3 1,626,106 + MMP_RS08665 0.47 +0.4 1,626,120 + MMP_RS08665 0.49 +0.5 1,626,150 + MMP_RS08665 0.54 +0.4 1,626,152 - MMP_RS08665 0.54 +0.1 1,626,152 - MMP_RS08665 0.54 +0.2 1,626,155 + MMP_RS08665 0.54 +0.5 1,626,155 + MMP_RS08665 0.54 -0.0 1,626,155 + MMP_RS08665 0.54 -0.4 1,626,156 - MMP_RS08665 0.55 +0.3 1,626,157 + MMP_RS08665 0.55 +0.5 1,626,189 - MMP_RS08665 0.60 +0.0 1,626,211 + MMP_RS08665 0.63 +0.3 1,626,306 + MMP_RS08665 0.78 +0.6 1,626,314 - MMP_RS08665 0.79 +0.1 1,626,314 - MMP_RS08665 0.79 +0.2 1,626,314 - MMP_RS08665 0.79 -0.2 1,626,342 + MMP_RS08665 0.83 +0.0 1,626,365 - MMP_RS08665 0.87 +0.3 1,626,483 - -0.1 1,626,488 - -0.2 1,626,489 + +0.0 1,626,489 + +0.2 1,626,490 - -0.5 1,626,491 + -0.4 1,626,492 - -0.5 1,626,518 + MMP_RS08670 0.10 -0.1 1,626,518 + MMP_RS08670 0.10 -0.3 1,626,529 + MMP_RS08670 0.12 -1.2 1,626,546 - MMP_RS08670 0.14 -0.3 1,626,592 + MMP_RS08670 0.21 -0.3 1,626,661 + MMP_RS08670 0.31 -0.2
Or see this region's nucleotide sequence