Experiment: Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS07835 and MMP_RS07840 are separated by 51 nucleotides MMP_RS07840 and MMP_RS07845 are separated by 42 nucleotides MMP_RS07845 and MMP_RS07850 are separated by 110 nucleotides MMP_RS07850 and MMP_RS07855 overlap by 4 nucleotides
MMP_RS07835: MMP_RS07835 - TldD/PmbA family protein, at 1,484,018 to 1,485,280
_RS07835
MMP_RS07840: MMP_RS07840 - ribonuclease III, at 1,485,332 to 1,486,021
_RS07840
MMP_RS07845: MMP_RS07845 - LL-diaminopimelate aminotransferase, at 1,486,064 to 1,487,314
_RS07845
MMP_RS07850: MMP_RS07850 - prephenate dehydratase, at 1,487,425 to 1,488,231
_RS07850
MMP_RS07855: MMP_RS07855 - class I SAM-dependent methyltransferase, at 1,488,228 to 1,488,938
_RS07855
Position (kb) 1486
1487
1488 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 1485.123 kb on + strand, within MMP_RS07835 at 1485.313 kb on - strand at 1485.370 kb on + strand at 1485.385 kb on + strand at 1485.395 kb on + strand at 1485.566 kb on - strand, within MMP_RS07840 at 1485.761 kb on + strand, within MMP_RS07840 at 1486.083 kb on + strand at 1486.477 kb on + strand, within MMP_RS07845 at 1486.557 kb on - strand, within MMP_RS07845 at 1486.755 kb on - strand, within MMP_RS07845 at 1487.346 kb on - strand at 1487.366 kb on + strand at 1487.387 kb on - strand at 1487.388 kb on + strand at 1487.455 kb on - strand at 1487.507 kb on - strand, within MMP_RS07850 at 1487.517 kb on + strand, within MMP_RS07850 at 1487.707 kb on + strand, within MMP_RS07850
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE remove 1,485,123 + MMP_RS07835 0.87 -0.4 1,485,313 - +0.5 1,485,370 + +0.2 1,485,385 + +0.3 1,485,395 + -0.0 1,485,566 - MMP_RS07840 0.34 +0.3 1,485,761 + MMP_RS07840 0.62 +0.1 1,486,083 + -4.0 1,486,477 + MMP_RS07845 0.33 -4.3 1,486,557 - MMP_RS07845 0.39 -4.3 1,486,755 - MMP_RS07845 0.55 -5.2 1,487,346 - +0.3 1,487,366 + +0.6 1,487,387 - -0.2 1,487,388 + +0.2 1,487,455 - -0.3 1,487,507 - MMP_RS07850 0.10 -0.1 1,487,517 + MMP_RS07850 0.11 +0.2 1,487,707 + MMP_RS07850 0.35 +0.4
Or see this region's nucleotide sequence