Experiment: Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS06930 and MMP_RS06935 are separated by 9 nucleotides MMP_RS06935 and MMP_RS06940 are separated by 4 nucleotides MMP_RS06940 and MMP_RS06945 are separated by 16 nucleotides MMP_RS06945 and MMP_RS06950 are separated by 129 nucleotides
MMP_RS06930: MMP_RS06930 - MBL fold metallo-hydrolase, at 1,324,489 to 1,325,289
_RS06930
MMP_RS06935: MMP_RS06935 - protease complex subunit PrcB family protein, at 1,325,299 to 1,325,775
_RS06935
MMP_RS06940: MMP_RS06940 - polyprenyl diphosphate synthase, at 1,325,780 to 1,326,574
_RS06940
MMP_RS06945: MMP_RS06945 - methanogenesis marker protein Mmp4/MtxX, at 1,326,591 to 1,327,322
_RS06945
MMP_RS06950: MMP_RS06950 - histone family protein, at 1,327,452 to 1,327,652
_RS06950
Position (kb)
1325
1326
1327 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 1324.924 kb on - strand, within MMP_RS06930 at 1324.925 kb on + strand, within MMP_RS06930 at 1325.080 kb on + strand, within MMP_RS06930 at 1325.081 kb on - strand, within MMP_RS06930 at 1325.082 kb on + strand, within MMP_RS06930 at 1325.082 kb on + strand, within MMP_RS06930 at 1325.082 kb on + strand, within MMP_RS06930 at 1325.103 kb on - strand, within MMP_RS06930 at 1325.103 kb on - strand, within MMP_RS06930 at 1325.107 kb on + strand, within MMP_RS06930 at 1325.107 kb on + strand, within MMP_RS06930 at 1325.107 kb on + strand, within MMP_RS06930 at 1325.110 kb on - strand, within MMP_RS06930 at 1325.141 kb on + strand, within MMP_RS06930 at 1325.241 kb on - strand at 1325.490 kb on + strand, within MMP_RS06935 at 1325.540 kb on - strand, within MMP_RS06935 at 1325.540 kb on - strand, within MMP_RS06935 at 1325.541 kb on + strand, within MMP_RS06935 at 1325.541 kb on + strand, within MMP_RS06935 at 1325.541 kb on + strand, within MMP_RS06935 at 1325.632 kb on - strand, within MMP_RS06935 at 1325.632 kb on - strand, within MMP_RS06935 at 1325.668 kb on - strand, within MMP_RS06935 at 1325.692 kb on + strand, within MMP_RS06935 at 1325.760 kb on - strand at 1325.768 kb on - strand at 1325.859 kb on - strand at 1325.859 kb on - strand at 1325.860 kb on + strand, within MMP_RS06940 at 1325.860 kb on + strand, within MMP_RS06940 at 1325.877 kb on + strand, within MMP_RS06940 at 1325.917 kb on - strand, within MMP_RS06940 at 1326.284 kb on - strand, within MMP_RS06940 at 1326.285 kb on + strand, within MMP_RS06940 at 1326.334 kb on - strand, within MMP_RS06940 at 1326.337 kb on - strand, within MMP_RS06940 at 1326.370 kb on - strand, within MMP_RS06940 at 1326.657 kb on + strand at 1326.660 kb on - strand at 1326.663 kb on + strand at 1326.734 kb on - strand at 1326.742 kb on + strand at 1326.774 kb on - strand at 1326.797 kb on + strand at 1326.797 kb on + strand at 1326.807 kb on - strand at 1326.999 kb on - strand at 1326.999 kb on - strand at 1327.023 kb on - strand at 1327.104 kb on + strand at 1327.318 kb on - strand at 1327.318 kb on - strand at 1327.319 kb on + strand at 1327.334 kb on - strand at 1327.346 kb on + strand at 1327.374 kb on + strand at 1327.376 kb on - strand at 1327.384 kb on - strand at 1327.384 kb on - strand at 1327.384 kb on - strand at 1327.384 kb on - strand at 1327.385 kb on + strand at 1327.385 kb on + strand at 1327.386 kb on - strand at 1327.387 kb on + strand at 1327.404 kb on - strand at 1327.405 kb on + strand at 1327.419 kb on - strand at 1327.422 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE remove 1,324,924 - MMP_RS06930 0.54 +0.4 1,324,925 + MMP_RS06930 0.54 +0.6 1,325,080 + MMP_RS06930 0.74 +0.3 1,325,081 - MMP_RS06930 0.74 -0.3 1,325,082 + MMP_RS06930 0.74 +0.2 1,325,082 + MMP_RS06930 0.74 +0.2 1,325,082 + MMP_RS06930 0.74 +0.1 1,325,103 - MMP_RS06930 0.77 +0.2 1,325,103 - MMP_RS06930 0.77 +3.4 1,325,107 + MMP_RS06930 0.77 -0.8 1,325,107 + MMP_RS06930 0.77 +0.7 1,325,107 + MMP_RS06930 0.77 +0.1 1,325,110 - MMP_RS06930 0.78 +0.5 1,325,141 + MMP_RS06930 0.81 +0.3 1,325,241 - -0.5 1,325,490 + MMP_RS06935 0.40 +0.5 1,325,540 - MMP_RS06935 0.51 -0.3 1,325,540 - MMP_RS06935 0.51 -0.0 1,325,541 + MMP_RS06935 0.51 -0.4 1,325,541 + MMP_RS06935 0.51 -1.0 1,325,541 + MMP_RS06935 0.51 +0.2 1,325,632 - MMP_RS06935 0.70 +0.4 1,325,632 - MMP_RS06935 0.70 +0.1 1,325,668 - MMP_RS06935 0.77 -0.4 1,325,692 + MMP_RS06935 0.82 +0.1 1,325,760 - +0.3 1,325,768 - -0.1 1,325,859 - -0.3 1,325,859 - +0.1 1,325,860 + MMP_RS06940 0.10 -1.3 1,325,860 + MMP_RS06940 0.10 -0.5 1,325,877 + MMP_RS06940 0.12 -0.9 1,325,917 - MMP_RS06940 0.17 -1.3 1,326,284 - MMP_RS06940 0.63 -2.7 1,326,285 + MMP_RS06940 0.64 -0.3 1,326,334 - MMP_RS06940 0.70 -1.3 1,326,337 - MMP_RS06940 0.70 -1.5 1,326,370 - MMP_RS06940 0.74 -0.9 1,326,657 + -3.7 1,326,660 - -1.9 1,326,663 + -1.1 1,326,734 - -2.2 1,326,742 + -1.1 1,326,774 - -2.4 1,326,797 + -1.1 1,326,797 + -1.3 1,326,807 - -2.5 1,326,999 - -1.8 1,326,999 - -2.7 1,327,023 - -2.1 1,327,104 + -4.4 1,327,318 - -2.8 1,327,318 - -2.0 1,327,319 + -2.4 1,327,334 - +3.0 1,327,346 + +0.2 1,327,374 + -0.6 1,327,376 - +0.3 1,327,384 - -0.2 1,327,384 - +0.4 1,327,384 - -0.0 1,327,384 - -0.3 1,327,385 + -0.2 1,327,385 + -0.3 1,327,386 - +0.4 1,327,387 + -0.2 1,327,404 - +0.5 1,327,405 + -0.1 1,327,419 - -0.9 1,327,422 - -0.1
Or see this region's nucleotide sequence