Experiment: Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS05865 and MMP_RS05870 are separated by 201 nucleotides MMP_RS05870 and MMP_RS05875 are separated by 209 nucleotides MMP_RS05875 and MMP_RS05880 overlap by 11 nucleotides MMP_RS05880 and MMP_RS05885 are separated by 175 nucleotides
MMP_RS05865: MMP_RS05865 - hypothetical protein, at 1,126,811 to 1,127,230
_RS05865
MMP_RS05870: MMP_RS05870 - Lrp/AsnC family transcriptional regulator, at 1,127,432 to 1,127,854
_RS05870
MMP_RS05875: MMP_RS05875 - hydroxyethylthiazole kinase, at 1,128,064 to 1,128,864
_RS05875
MMP_RS05880: MMP_RS05880 - thiamine phosphate synthase, at 1,128,854 to 1,129,477
_RS05880
MMP_RS05885: MMP_RS05885 - ferredoxin family protein, at 1,129,653 to 1,129,898
_RS05885
Position (kb)
1128
1129 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1127.212 kb on + strand at 1127.215 kb on - strand at 1127.220 kb on + strand at 1127.258 kb on - strand at 1127.269 kb on + strand at 1127.316 kb on + strand at 1127.342 kb on - strand at 1127.438 kb on - strand at 1127.875 kb on - strand at 1127.875 kb on - strand at 1127.876 kb on + strand at 1127.876 kb on + strand at 1127.929 kb on - strand at 1127.930 kb on + strand at 1127.932 kb on + strand at 1127.933 kb on - strand at 1127.934 kb on + strand at 1127.952 kb on - strand at 1128.015 kb on - strand at 1128.015 kb on - strand at 1128.015 kb on - strand at 1128.015 kb on - strand at 1128.018 kb on + strand at 1128.018 kb on + strand at 1128.030 kb on + strand at 1128.040 kb on + strand at 1128.074 kb on - strand at 1128.075 kb on + strand at 1128.134 kb on + strand at 1128.140 kb on - strand at 1128.477 kb on + strand, within MMP_RS05875 at 1128.490 kb on - strand, within MMP_RS05875 at 1128.578 kb on - strand, within MMP_RS05875 at 1128.579 kb on + strand, within MMP_RS05875 at 1128.658 kb on + strand, within MMP_RS05875 at 1129.275 kb on - strand at 1129.470 kb on - strand at 1129.514 kb on + strand at 1129.600 kb on + strand at 1129.606 kb on - strand at 1129.607 kb on + strand at 1129.660 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE remove 1,127,212 + +0.6 1,127,215 - -0.2 1,127,220 + +0.1 1,127,258 - +0.1 1,127,269 + +0.3 1,127,316 + +0.0 1,127,342 - -0.0 1,127,438 - -0.3 1,127,875 - +0.1 1,127,875 - -0.1 1,127,876 + -0.6 1,127,876 + -0.4 1,127,929 - -0.9 1,127,930 + -0.2 1,127,932 + +0.1 1,127,933 - +0.4 1,127,934 + +0.0 1,127,952 - -0.4 1,128,015 - +0.1 1,128,015 - +0.2 1,128,015 - +0.4 1,128,015 - +0.3 1,128,018 + +0.1 1,128,018 + +0.3 1,128,030 + -0.4 1,128,040 + +0.7 1,128,074 - -2.2 1,128,075 + -0.6 1,128,134 + -1.3 1,128,140 - -2.1 1,128,477 + MMP_RS05875 0.52 -0.9 1,128,490 - MMP_RS05875 0.53 -1.7 1,128,578 - MMP_RS05875 0.64 -1.6 1,128,579 + MMP_RS05875 0.64 -0.6 1,128,658 + MMP_RS05875 0.74 -0.1 1,129,275 - -1.4 1,129,470 - -3.4 1,129,514 + +0.1 1,129,600 + +0.4 1,129,606 - -0.0 1,129,607 + +0.2 1,129,660 - +0.4
Or see this region's nucleotide sequence