Experiment: Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS05275 and MMP_RS05280 are separated by 160 nucleotides MMP_RS05280 and MMP_RS05285 overlap by 8 nucleotides
MMP_RS05275: MMP_RS05275 - AI-2E family transporter, at 1,008,301 to 1,009,266
_RS05275
MMP_RS05280: MMP_RS05280 - COG1361 S-layer family protein, at 1,009,427 to 1,010,998
_RS05280
MMP_RS05285: MMP_RS05285 - MMPL family transporter, at 1,010,991 to 1,012,157
_RS05285
Position (kb)
1009
1010
1011 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1
2 at 1008.560 kb on - strand, within MMP_RS05275 at 1008.561 kb on + strand, within MMP_RS05275 at 1008.565 kb on - strand, within MMP_RS05275 at 1008.565 kb on - strand, within MMP_RS05275 at 1008.565 kb on - strand, within MMP_RS05275 at 1008.581 kb on - strand, within MMP_RS05275 at 1008.582 kb on + strand, within MMP_RS05275 at 1008.582 kb on + strand, within MMP_RS05275 at 1008.582 kb on + strand, within MMP_RS05275 at 1008.620 kb on + strand, within MMP_RS05275 at 1008.642 kb on - strand, within MMP_RS05275 at 1008.771 kb on - strand, within MMP_RS05275 at 1008.797 kb on - strand, within MMP_RS05275 at 1008.810 kb on - strand, within MMP_RS05275 at 1008.979 kb on - strand, within MMP_RS05275 at 1009.029 kb on - strand, within MMP_RS05275 at 1009.052 kb on + strand, within MMP_RS05275 at 1009.060 kb on - strand, within MMP_RS05275 at 1009.093 kb on - strand, within MMP_RS05275 at 1009.213 kb on + strand at 1009.366 kb on - strand at 1009.372 kb on - strand at 1009.373 kb on + strand at 1009.471 kb on + strand at 1009.475 kb on + strand at 1009.475 kb on + strand at 1009.475 kb on + strand at 1009.477 kb on + strand at 1009.480 kb on + strand at 1009.485 kb on + strand at 1009.511 kb on - strand at 1009.516 kb on + strand at 1009.555 kb on - strand at 1009.575 kb on - strand at 1009.575 kb on - strand at 1009.615 kb on - strand, within MMP_RS05280 at 1009.627 kb on - strand, within MMP_RS05280 at 1009.851 kb on - strand, within MMP_RS05280 at 1009.934 kb on - strand, within MMP_RS05280 at 1009.935 kb on + strand, within MMP_RS05280 at 1009.937 kb on + strand, within MMP_RS05280 at 1009.995 kb on + strand, within MMP_RS05280 at 1010.005 kb on + strand, within MMP_RS05280 at 1010.250 kb on + strand, within MMP_RS05280 at 1010.313 kb on + strand, within MMP_RS05280 at 1010.316 kb on - strand, within MMP_RS05280 at 1010.316 kb on - strand, within MMP_RS05280 at 1010.491 kb on - strand, within MMP_RS05280 at 1010.575 kb on - strand, within MMP_RS05280 at 1010.577 kb on - strand, within MMP_RS05280 at 1010.577 kb on - strand, within MMP_RS05280 at 1010.592 kb on - strand, within MMP_RS05280 at 1010.709 kb on - strand, within MMP_RS05280 at 1010.736 kb on + strand, within MMP_RS05280 at 1010.781 kb on + strand, within MMP_RS05280 at 1010.810 kb on - strand, within MMP_RS05280 at 1010.816 kb on - strand, within MMP_RS05280 at 1011.228 kb on - strand, within MMP_RS05285 at 1011.228 kb on - strand, within MMP_RS05285 at 1011.243 kb on - strand, within MMP_RS05285 at 1011.328 kb on + strand, within MMP_RS05285 at 1011.460 kb on - strand, within MMP_RS05285 at 1011.469 kb on + strand, within MMP_RS05285 at 1011.670 kb on - strand, within MMP_RS05285 at 1011.747 kb on - strand, within MMP_RS05285 at 1011.784 kb on - strand, within MMP_RS05285 at 1011.784 kb on - strand, within MMP_RS05285 at 1011.785 kb on + strand, within MMP_RS05285 at 1011.828 kb on + strand, within MMP_RS05285 at 1011.944 kb on - strand, within MMP_RS05285
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE remove 1,008,560 - MMP_RS05275 0.27 +0.0 1,008,561 + MMP_RS05275 0.27 -0.3 1,008,565 - MMP_RS05275 0.27 +0.1 1,008,565 - MMP_RS05275 0.27 +0.4 1,008,565 - MMP_RS05275 0.27 -0.3 1,008,581 - MMP_RS05275 0.29 +0.0 1,008,582 + MMP_RS05275 0.29 +0.2 1,008,582 + MMP_RS05275 0.29 -0.0 1,008,582 + MMP_RS05275 0.29 -0.1 1,008,620 + MMP_RS05275 0.33 -0.1 1,008,642 - MMP_RS05275 0.35 +0.3 1,008,771 - MMP_RS05275 0.49 -0.1 1,008,797 - MMP_RS05275 0.51 +0.5 1,008,810 - MMP_RS05275 0.53 +0.6 1,008,979 - MMP_RS05275 0.70 +0.1 1,009,029 - MMP_RS05275 0.75 -0.1 1,009,052 + MMP_RS05275 0.78 +0.1 1,009,060 - MMP_RS05275 0.79 +2.7 1,009,093 - MMP_RS05275 0.82 +0.1 1,009,213 + -0.9 1,009,366 - -0.6 1,009,372 - -0.3 1,009,373 + +0.3 1,009,471 + -3.8 1,009,475 + -1.4 1,009,475 + -0.5 1,009,475 + -1.0 1,009,477 + -5.4 1,009,480 + -3.2 1,009,485 + +0.0 1,009,511 - -2.1 1,009,516 + -3.2 1,009,555 - -4.3 1,009,575 - -4.8 1,009,575 - -4.0 1,009,615 - MMP_RS05280 0.12 -3.5 1,009,627 - MMP_RS05280 0.13 -2.9 1,009,851 - MMP_RS05280 0.27 -3.3 1,009,934 - MMP_RS05280 0.32 -4.3 1,009,935 + MMP_RS05280 0.32 -3.1 1,009,937 + MMP_RS05280 0.32 -3.3 1,009,995 + MMP_RS05280 0.36 -2.5 1,010,005 + MMP_RS05280 0.37 -3.5 1,010,250 + MMP_RS05280 0.52 -3.7 1,010,313 + MMP_RS05280 0.56 -3.9 1,010,316 - MMP_RS05280 0.57 -4.2 1,010,316 - MMP_RS05280 0.57 -4.6 1,010,491 - MMP_RS05280 0.68 -4.2 1,010,575 - MMP_RS05280 0.73 -3.8 1,010,577 - MMP_RS05280 0.73 -3.9 1,010,577 - MMP_RS05280 0.73 -4.4 1,010,592 - MMP_RS05280 0.74 -4.2 1,010,709 - MMP_RS05280 0.82 -3.5 1,010,736 + MMP_RS05280 0.83 -4.5 1,010,781 + MMP_RS05280 0.86 -4.1 1,010,810 - MMP_RS05280 0.88 -3.5 1,010,816 - MMP_RS05280 0.88 -5.6 1,011,228 - MMP_RS05285 0.20 -4.4 1,011,228 - MMP_RS05285 0.20 -3.3 1,011,243 - MMP_RS05285 0.22 -3.9 1,011,328 + MMP_RS05285 0.29 -3.3 1,011,460 - MMP_RS05285 0.40 -4.7 1,011,469 + MMP_RS05285 0.41 -4.2 1,011,670 - MMP_RS05285 0.58 -5.8 1,011,747 - MMP_RS05285 0.65 -3.3 1,011,784 - MMP_RS05285 0.68 -3.6 1,011,784 - MMP_RS05285 0.68 -3.8 1,011,785 + MMP_RS05285 0.68 -3.4 1,011,828 + MMP_RS05285 0.72 -4.5 1,011,944 - MMP_RS05285 0.82 -4.6
Or see this region's nucleotide sequence