Experiment: Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS01575 and MMP_RS01580 are separated by 158 nucleotides MMP_RS01580 and MMP_RS01585 are separated by 123 nucleotides
MMP_RS01575: MMP_RS01575 - aldolase, at 293,196 to 294,122
_RS01575
MMP_RS01580: MMP_RS01580 - PQQ-binding-like beta-propeller repeat protein, at 294,281 to 295,129
_RS01580
MMP_RS01585: MMP_RS01585 - homoserine kinase, at 295,253 to 296,158
_RS01585
Position (kb) 294
295
296 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 293.413 kb on + strand, within MMP_RS01575 at 293.420 kb on - strand, within MMP_RS01575 at 293.438 kb on - strand, within MMP_RS01575 at 293.487 kb on - strand, within MMP_RS01575 at 293.682 kb on - strand, within MMP_RS01575 at 293.682 kb on - strand, within MMP_RS01575 at 293.838 kb on + strand, within MMP_RS01575 at 294.029 kb on - strand, within MMP_RS01575 at 294.137 kb on - strand at 294.145 kb on + strand at 294.152 kb on - strand at 294.153 kb on + strand at 294.164 kb on - strand at 294.164 kb on - strand at 294.172 kb on - strand at 294.195 kb on + strand at 294.224 kb on + strand at 294.224 kb on + strand at 294.225 kb on - strand at 294.226 kb on + strand at 294.226 kb on + strand at 294.226 kb on + strand at 294.226 kb on + strand at 294.227 kb on - strand at 294.228 kb on + strand at 294.241 kb on + strand at 294.241 kb on + strand at 294.241 kb on + strand at 294.244 kb on - strand at 294.244 kb on - strand at 294.245 kb on + strand at 294.245 kb on + strand at 294.245 kb on + strand at 294.245 kb on + strand at 294.245 kb on + strand at 294.338 kb on + strand at 294.338 kb on + strand at 294.377 kb on + strand, within MMP_RS01580 at 294.377 kb on + strand, within MMP_RS01580 at 294.398 kb on + strand, within MMP_RS01580 at 294.398 kb on + strand, within MMP_RS01580 at 294.459 kb on - strand, within MMP_RS01580 at 294.470 kb on + strand, within MMP_RS01580 at 294.515 kb on + strand, within MMP_RS01580 at 294.637 kb on + strand, within MMP_RS01580 at 294.752 kb on - strand, within MMP_RS01580 at 294.753 kb on + strand, within MMP_RS01580 at 294.870 kb on - strand, within MMP_RS01580 at 294.966 kb on + strand, within MMP_RS01580 at 295.055 kb on + strand at 295.131 kb on + strand at 295.195 kb on + strand at 295.270 kb on + strand at 295.447 kb on + strand at 296.009 kb on + strand at 296.009 kb on + strand at 296.064 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE remove 293,413 + MMP_RS01575 0.23 -0.8 293,420 - MMP_RS01575 0.24 -0.2 293,438 - MMP_RS01575 0.26 -0.3 293,487 - MMP_RS01575 0.31 +2.2 293,682 - MMP_RS01575 0.52 +0.1 293,682 - MMP_RS01575 0.52 +0.0 293,838 + MMP_RS01575 0.69 -0.4 294,029 - MMP_RS01575 0.90 -0.4 294,137 - +0.1 294,145 + -0.1 294,152 - -0.2 294,153 + +0.0 294,164 - -0.2 294,164 - -0.0 294,172 - -0.2 294,195 + +0.3 294,224 + -0.5 294,224 + -0.3 294,225 - -0.8 294,226 + -0.1 294,226 + -0.6 294,226 + -0.6 294,226 + -0.1 294,227 - -0.6 294,228 + -1.1 294,241 + -0.6 294,241 + -0.5 294,241 + -0.6 294,244 - -0.7 294,244 - -0.2 294,245 + -1.1 294,245 + -1.1 294,245 + -0.2 294,245 + -0.6 294,245 + -1.1 294,338 + -0.5 294,338 + -0.5 294,377 + MMP_RS01580 0.11 -0.7 294,377 + MMP_RS01580 0.11 -0.8 294,398 + MMP_RS01580 0.14 -0.5 294,398 + MMP_RS01580 0.14 -0.7 294,459 - MMP_RS01580 0.21 -0.4 294,470 + MMP_RS01580 0.22 +0.1 294,515 + MMP_RS01580 0.28 -0.2 294,637 + MMP_RS01580 0.42 -0.4 294,752 - MMP_RS01580 0.55 -0.4 294,753 + MMP_RS01580 0.56 -0.8 294,870 - MMP_RS01580 0.69 -1.5 294,966 + MMP_RS01580 0.81 -0.6 295,055 + -1.1 295,131 + -0.1 295,195 + -3.4 295,270 + -2.8 295,447 + -2.2 296,009 + -3.3 296,009 + -3.6 296,064 - -0.3
Or see this region's nucleotide sequence