Experiment: Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS01170 and MMP_RS01175 are separated by 11 nucleotides MMP_RS01175 and MMP_RS01180 are separated by 11 nucleotides MMP_RS01180 and MMP_RS01185 are separated by 115 nucleotides
MMP_RS01170: MMP_RS01170 - glycine--tRNA ligase, at 218,916 to 220,640
_RS01170
MMP_RS01175: MMP_RS01175 - triphosphoribosyl-dephospho-CoA synthase, at 220,652 to 221,533
_RS01175
MMP_RS01180: MMP_RS01180 - XTP/dITP diphosphatase, at 221,545 to 222,096
_RS01180
MMP_RS01185: MMP_RS01185 - hypothetical protein, at 222,212 to 222,622
_RS01185
Position (kb) 220
221
222 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 219.809 kb on + strand at 219.924 kb on - strand at 220.486 kb on - strand at 220.895 kb on - strand, within MMP_RS01175 at 220.948 kb on + strand, within MMP_RS01175 at 220.951 kb on + strand, within MMP_RS01175 at 220.977 kb on - strand, within MMP_RS01175 at 221.236 kb on - strand, within MMP_RS01175 at 221.241 kb on + strand, within MMP_RS01175 at 221.568 kb on - strand at 221.759 kb on - strand, within MMP_RS01180 at 221.915 kb on + strand, within MMP_RS01180 at 222.063 kb on - strand at 222.086 kb on - strand at 222.097 kb on + strand at 222.134 kb on - strand at 222.135 kb on + strand at 222.142 kb on + strand at 222.158 kb on - strand at 222.158 kb on - strand at 222.158 kb on - strand at 222.159 kb on + strand at 222.171 kb on + strand at 222.186 kb on + strand at 222.188 kb on - strand at 222.444 kb on - strand, within MMP_RS01185 at 222.444 kb on - strand, within MMP_RS01185 at 222.447 kb on + strand, within MMP_RS01185 at 222.449 kb on + strand, within MMP_RS01185 at 222.475 kb on - strand, within MMP_RS01185
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE remove 219,809 + -2.6 219,924 - -2.6 220,486 - -0.8 220,895 - MMP_RS01175 0.28 -3.5 220,948 + MMP_RS01175 0.34 -3.4 220,951 + MMP_RS01175 0.34 -2.8 220,977 - MMP_RS01175 0.37 -3.5 221,236 - MMP_RS01175 0.66 -3.5 221,241 + MMP_RS01175 0.67 -5.6 221,568 - -0.1 221,759 - MMP_RS01180 0.39 -0.2 221,915 + MMP_RS01180 0.67 +0.5 222,063 - -0.0 222,086 - -0.0 222,097 + +0.6 222,134 - +0.6 222,135 + +0.2 222,142 + +0.1 222,158 - +0.2 222,158 - +0.1 222,158 - +0.1 222,159 + +0.0 222,171 + +0.4 222,186 + -0.1 222,188 - +0.4 222,444 - MMP_RS01185 0.56 -0.1 222,444 - MMP_RS01185 0.56 +0.1 222,447 + MMP_RS01185 0.57 -0.2 222,449 + MMP_RS01185 0.58 +0.1 222,475 - MMP_RS01185 0.64 +0.1
Or see this region's nucleotide sequence