Experiment: Recovery broth with Erythromycin 1e-05 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS16215 and BBR_RS16220 are separated by 5 nucleotides BBR_RS16220 and BBR_RS16225 are separated by 258 nucleotides
BBR_RS16215: BBR_RS16215 - restriction enzyme, at 1,384,505 to 1,386,592
_RS16215
BBR_RS16220: BBR_RS16220 - DNA cytosine methyltransferase, at 1,386,598 to 1,387,647
_RS16220
BBR_RS16225: BBR_RS16225 - type IA DNA topoisomerase, at 1,387,906 to 1,389,837
_RS16225
Position (kb)
1386
1387
1388 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1385.623 kb on - strand, within BBR_RS16215 at 1385.623 kb on - strand, within BBR_RS16215 at 1385.654 kb on + strand, within BBR_RS16215 at 1385.663 kb on + strand, within BBR_RS16215 at 1385.666 kb on + strand, within BBR_RS16215 at 1385.729 kb on - strand, within BBR_RS16215 at 1385.766 kb on + strand, within BBR_RS16215 at 1385.792 kb on + strand, within BBR_RS16215 at 1385.894 kb on - strand, within BBR_RS16215 at 1385.894 kb on - strand, within BBR_RS16215 at 1385.894 kb on - strand, within BBR_RS16215 at 1385.894 kb on - strand, within BBR_RS16215 at 1385.896 kb on + strand, within BBR_RS16215 at 1385.904 kb on - strand, within BBR_RS16215 at 1385.904 kb on - strand, within BBR_RS16215 at 1385.906 kb on + strand, within BBR_RS16215 at 1385.906 kb on + strand, within BBR_RS16215 at 1385.914 kb on - strand, within BBR_RS16215 at 1385.923 kb on + strand, within BBR_RS16215 at 1386.042 kb on - strand, within BBR_RS16215 at 1386.049 kb on - strand, within BBR_RS16215 at 1386.079 kb on - strand, within BBR_RS16215 at 1386.089 kb on - strand, within BBR_RS16215 at 1386.094 kb on + strand, within BBR_RS16215 at 1386.102 kb on - strand, within BBR_RS16215 at 1386.143 kb on + strand, within BBR_RS16215 at 1386.151 kb on - strand, within BBR_RS16215 at 1386.151 kb on - strand, within BBR_RS16215 at 1386.168 kb on - strand, within BBR_RS16215 at 1386.181 kb on + strand, within BBR_RS16215 at 1386.198 kb on - strand, within BBR_RS16215 at 1386.218 kb on - strand, within BBR_RS16215 at 1386.218 kb on - strand, within BBR_RS16215 at 1386.218 kb on - strand, within BBR_RS16215 at 1386.245 kb on - strand, within BBR_RS16215 at 1386.245 kb on - strand, within BBR_RS16215 at 1386.271 kb on - strand, within BBR_RS16215 at 1386.422 kb on - strand at 1386.436 kb on - strand at 1386.480 kb on + strand at 1387.366 kb on + strand, within BBR_RS16220 at 1387.671 kb on - strand at 1387.719 kb on + strand at 1387.729 kb on + strand at 1387.736 kb on - strand at 1387.775 kb on + strand at 1387.775 kb on + strand at 1387.783 kb on - strand at 1387.790 kb on + strand at 1387.798 kb on + strand at 1387.798 kb on - strand at 1387.867 kb on + strand at 1387.869 kb on + strand at 1387.869 kb on + strand at 1387.879 kb on + strand at 1388.058 kb on - strand at 1388.074 kb on - strand at 1388.147 kb on + strand, within BBR_RS16225 at 1388.185 kb on + strand, within BBR_RS16225 at 1388.185 kb on + strand, within BBR_RS16225 at 1388.198 kb on - strand, within BBR_RS16225 at 1388.226 kb on - strand, within BBR_RS16225 at 1388.237 kb on + strand, within BBR_RS16225 at 1388.249 kb on + strand, within BBR_RS16225 at 1388.249 kb on + strand, within BBR_RS16225 at 1388.249 kb on + strand, within BBR_RS16225 at 1388.257 kb on - strand, within BBR_RS16225 at 1388.257 kb on - strand, within BBR_RS16225 at 1388.257 kb on - strand, within BBR_RS16225 at 1388.257 kb on - strand, within BBR_RS16225 at 1388.257 kb on - strand, within BBR_RS16225 at 1388.257 kb on - strand, within BBR_RS16225 at 1388.262 kb on - strand, within BBR_RS16225 at 1388.326 kb on + strand, within BBR_RS16225 at 1388.326 kb on + strand, within BBR_RS16225 at 1388.341 kb on + strand, within BBR_RS16225 at 1388.341 kb on + strand, within BBR_RS16225 at 1388.387 kb on - strand, within BBR_RS16225 at 1388.397 kb on - strand, within BBR_RS16225 at 1388.419 kb on - strand, within BBR_RS16225 at 1388.444 kb on + strand, within BBR_RS16225 at 1388.465 kb on + strand, within BBR_RS16225 at 1388.549 kb on + strand, within BBR_RS16225 at 1388.551 kb on + strand, within BBR_RS16225 at 1388.556 kb on + strand, within BBR_RS16225 at 1388.559 kb on - strand, within BBR_RS16225 at 1388.615 kb on + strand, within BBR_RS16225 at 1388.615 kb on + strand, within BBR_RS16225 at 1388.615 kb on + strand, within BBR_RS16225 at 1388.615 kb on + strand, within BBR_RS16225 at 1388.615 kb on + strand, within BBR_RS16225 at 1388.623 kb on - strand, within BBR_RS16225 at 1388.623 kb on - strand, within BBR_RS16225
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Erythromycin 1e-05 mM remove 1,385,623 - BBR_RS16215 0.54 -0.7 1,385,623 - BBR_RS16215 0.54 -0.8 1,385,654 + BBR_RS16215 0.55 +0.1 1,385,663 + BBR_RS16215 0.55 +0.1 1,385,666 + BBR_RS16215 0.56 -1.8 1,385,729 - BBR_RS16215 0.59 -0.1 1,385,766 + BBR_RS16215 0.60 +0.6 1,385,792 + BBR_RS16215 0.62 -1.3 1,385,894 - BBR_RS16215 0.67 +0.5 1,385,894 - BBR_RS16215 0.67 +0.1 1,385,894 - BBR_RS16215 0.67 +0.9 1,385,894 - BBR_RS16215 0.67 -0.9 1,385,896 + BBR_RS16215 0.67 -1.8 1,385,904 - BBR_RS16215 0.67 +0.3 1,385,904 - BBR_RS16215 0.67 -0.5 1,385,906 + BBR_RS16215 0.67 +0.8 1,385,906 + BBR_RS16215 0.67 -0.3 1,385,914 - BBR_RS16215 0.67 -1.6 1,385,923 + BBR_RS16215 0.68 +0.4 1,386,042 - BBR_RS16215 0.74 -2.0 1,386,049 - BBR_RS16215 0.74 -0.3 1,386,079 - BBR_RS16215 0.75 +0.7 1,386,089 - BBR_RS16215 0.76 +0.3 1,386,094 + BBR_RS16215 0.76 -0.4 1,386,102 - BBR_RS16215 0.76 +0.2 1,386,143 + BBR_RS16215 0.78 -0.4 1,386,151 - BBR_RS16215 0.79 +0.6 1,386,151 - BBR_RS16215 0.79 -0.1 1,386,168 - BBR_RS16215 0.80 -0.7 1,386,181 + BBR_RS16215 0.80 -0.2 1,386,198 - BBR_RS16215 0.81 +0.2 1,386,218 - BBR_RS16215 0.82 +0.3 1,386,218 - BBR_RS16215 0.82 +0.5 1,386,218 - BBR_RS16215 0.82 +0.3 1,386,245 - BBR_RS16215 0.83 +0.6 1,386,245 - BBR_RS16215 0.83 +0.8 1,386,271 - BBR_RS16215 0.85 -1.7 1,386,422 - -0.2 1,386,436 - +0.5 1,386,480 + -0.1 1,387,366 + BBR_RS16220 0.73 -3.1 1,387,671 - +0.5 1,387,719 + -0.5 1,387,729 + -0.2 1,387,736 - +0.2 1,387,775 + -0.6 1,387,775 + -0.6 1,387,783 - -0.1 1,387,790 + -0.3 1,387,798 + -0.9 1,387,798 - +0.5 1,387,867 + -0.2 1,387,869 + -0.2 1,387,869 + +0.1 1,387,879 + -0.1 1,388,058 - -0.7 1,388,074 - +0.4 1,388,147 + BBR_RS16225 0.12 +2.1 1,388,185 + BBR_RS16225 0.14 +0.1 1,388,185 + BBR_RS16225 0.14 -0.2 1,388,198 - BBR_RS16225 0.15 -1.2 1,388,226 - BBR_RS16225 0.17 +0.3 1,388,237 + BBR_RS16225 0.17 -0.1 1,388,249 + BBR_RS16225 0.18 +1.0 1,388,249 + BBR_RS16225 0.18 -0.1 1,388,249 + BBR_RS16225 0.18 +0.5 1,388,257 - BBR_RS16225 0.18 +0.4 1,388,257 - BBR_RS16225 0.18 +0.1 1,388,257 - BBR_RS16225 0.18 +0.5 1,388,257 - BBR_RS16225 0.18 -1.3 1,388,257 - BBR_RS16225 0.18 +0.2 1,388,257 - BBR_RS16225 0.18 -0.3 1,388,262 - BBR_RS16225 0.18 -0.3 1,388,326 + BBR_RS16225 0.22 +0.0 1,388,326 + BBR_RS16225 0.22 +0.2 1,388,341 + BBR_RS16225 0.23 -0.4 1,388,341 + BBR_RS16225 0.23 -0.3 1,388,387 - BBR_RS16225 0.25 -1.6 1,388,397 - BBR_RS16225 0.25 -0.2 1,388,419 - BBR_RS16225 0.27 -0.6 1,388,444 + BBR_RS16225 0.28 +0.2 1,388,465 + BBR_RS16225 0.29 -1.2 1,388,549 + BBR_RS16225 0.33 -0.1 1,388,551 + BBR_RS16225 0.33 -0.7 1,388,556 + BBR_RS16225 0.34 +0.6 1,388,559 - BBR_RS16225 0.34 -0.6 1,388,615 + BBR_RS16225 0.37 +0.3 1,388,615 + BBR_RS16225 0.37 +0.3 1,388,615 + BBR_RS16225 0.37 -0.0 1,388,615 + BBR_RS16225 0.37 -0.1 1,388,615 + BBR_RS16225 0.37 -0.3 1,388,623 - BBR_RS16225 0.37 -0.2 1,388,623 - BBR_RS16225 0.37 -0.1
Or see this region's nucleotide sequence