Experiment: nitrate 25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Synpcc7942_1006 and fumC are separated by 780 nucleotides fumC and purU are separated by 36 nucleotides
Synpcc7942_1006: Synpcc7942_1006 - TPR repeat, at 1,017,044 to 1,018,123
_1006
Synpcc7942_1007: fumC - fumarate hydratase, at 1,018,904 to 1,020,295
fumC
Synpcc7942_1008: purU - formyltetrahydrofolate deformylase, at 1,020,332 to 1,021,186
purU
Position (kb) 1018
1019
1020
1021 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 1018.052 kb on + strand at 1018.145 kb on + strand at 1018.325 kb on + strand at 1018.510 kb on + strand at 1018.510 kb on + strand at 1018.518 kb on - strand at 1018.518 kb on - strand at 1018.608 kb on - strand at 1019.191 kb on - strand, within fumC at 1019.262 kb on + strand, within fumC at 1019.270 kb on - strand, within fumC at 1019.270 kb on - strand, within fumC at 1019.270 kb on - strand, within fumC at 1019.423 kb on + strand, within fumC at 1019.493 kb on + strand, within fumC at 1019.606 kb on - strand, within fumC at 1019.634 kb on + strand, within fumC at 1019.706 kb on - strand, within fumC at 1019.706 kb on - strand, within fumC at 1019.707 kb on - strand, within fumC at 1019.708 kb on - strand, within fumC at 1019.780 kb on + strand, within fumC at 1019.782 kb on + strand, within fumC at 1019.788 kb on - strand, within fumC at 1019.796 kb on + strand, within fumC at 1019.799 kb on - strand, within fumC at 1019.926 kb on - strand, within fumC at 1019.936 kb on - strand, within fumC at 1020.150 kb on - strand, within fumC at 1020.203 kb on + strand at 1020.219 kb on + strand at 1020.219 kb on + strand at 1020.252 kb on - strand at 1020.280 kb on - strand at 1020.338 kb on - strand at 1020.348 kb on + strand at 1020.365 kb on - strand at 1020.422 kb on + strand, within purU at 1020.472 kb on + strand, within purU at 1020.521 kb on + strand, within purU at 1020.528 kb on + strand, within purU at 1020.571 kb on - strand, within purU at 1020.917 kb on - strand, within purU at 1020.955 kb on + strand, within purU at 1020.957 kb on + strand, within purU at 1021.033 kb on - strand, within purU at 1021.033 kb on - strand, within purU at 1021.054 kb on - strand, within purU at 1021.083 kb on + strand, within purU at 1021.152 kb on - strand at 1021.157 kb on + strand at 1021.227 kb on + strand at 1021.268 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrate 25 mM remove 1,018,052 + +2.0 1,018,145 + -1.0 1,018,325 + -0.5 1,018,510 + -2.6 1,018,510 + +0.2 1,018,518 - -2.0 1,018,518 - +1.8 1,018,608 - +3.0 1,019,191 - fumC Synpcc7942_1007 0.21 -0.0 1,019,262 + fumC Synpcc7942_1007 0.26 -3.4 1,019,270 - fumC Synpcc7942_1007 0.26 +3.2 1,019,270 - fumC Synpcc7942_1007 0.26 -1.8 1,019,270 - fumC Synpcc7942_1007 0.26 -2.0 1,019,423 + fumC Synpcc7942_1007 0.37 -2.6 1,019,493 + fumC Synpcc7942_1007 0.42 -0.3 1,019,606 - fumC Synpcc7942_1007 0.50 -0.8 1,019,634 + fumC Synpcc7942_1007 0.52 -1.6 1,019,706 - fumC Synpcc7942_1007 0.58 -0.5 1,019,706 - fumC Synpcc7942_1007 0.58 -2.0 1,019,707 - fumC Synpcc7942_1007 0.58 -2.4 1,019,708 - fumC Synpcc7942_1007 0.58 -0.0 1,019,780 + fumC Synpcc7942_1007 0.63 -1.0 1,019,782 + fumC Synpcc7942_1007 0.63 -1.0 1,019,788 - fumC Synpcc7942_1007 0.64 -2.6 1,019,796 + fumC Synpcc7942_1007 0.64 -2.6 1,019,799 - fumC Synpcc7942_1007 0.64 -0.0 1,019,926 - fumC Synpcc7942_1007 0.73 -2.8 1,019,936 - fumC Synpcc7942_1007 0.74 -2.0 1,020,150 - fumC Synpcc7942_1007 0.90 -1.0 1,020,203 + -1.0 1,020,219 + -0.0 1,020,219 + -2.6 1,020,252 - -1.6 1,020,280 - -0.5 1,020,338 - -0.6 1,020,348 + +1.7 1,020,365 - +1.2 1,020,422 + purU Synpcc7942_1008 0.11 +3.7 1,020,472 + purU Synpcc7942_1008 0.16 +3.5 1,020,521 + purU Synpcc7942_1008 0.22 +1.8 1,020,528 + purU Synpcc7942_1008 0.23 -2.0 1,020,571 - purU Synpcc7942_1008 0.28 -3.0 1,020,917 - purU Synpcc7942_1008 0.68 -1.6 1,020,955 + purU Synpcc7942_1008 0.73 -1.0 1,020,957 + purU Synpcc7942_1008 0.73 -1.0 1,021,033 - purU Synpcc7942_1008 0.82 -2.6 1,021,033 - purU Synpcc7942_1008 0.82 -2.6 1,021,054 - purU Synpcc7942_1008 0.84 -2.0 1,021,083 + purU Synpcc7942_1008 0.88 +1.1 1,021,152 - -1.6 1,021,157 + -1.0 1,021,227 + +1.9 1,021,268 - -1.0
Or see this region's nucleotide sequence