Experiment: Cholin acetate 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_6040 and Pf1N1B4_6041 are separated by 249 nucleotides Pf1N1B4_6041 and Pf1N1B4_6042 are separated by 194 nucleotides Pf1N1B4_6042 and Pf1N1B4_6043 are separated by 113 nucleotides
Pf1N1B4_6040 - FIG00956858: hypothetical protein, at 503,778 to 504,143
_6040
Pf1N1B4_6041 - Carbon storage regulator, at 504,393 to 504,587
_6041
Pf1N1B4_6042 - Probable transmembrane protein, at 504,782 to 505,237
_6042
Pf1N1B4_6043 - membrane protein, putative, at 505,351 to 506,565
_6043
Position (kb) 504
505
506 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 503.889 kb on - strand, within Pf1N1B4_6040 at 503.889 kb on - strand, within Pf1N1B4_6040 at 503.948 kb on + strand at 503.949 kb on - strand, within Pf1N1B4_6040 at 503.949 kb on - strand at 503.949 kb on - strand, within Pf1N1B4_6040 at 503.949 kb on - strand, within Pf1N1B4_6040 at 503.949 kb on - strand, within Pf1N1B4_6040 at 503.949 kb on - strand, within Pf1N1B4_6040 at 503.949 kb on - strand, within Pf1N1B4_6040 at 503.949 kb on - strand, within Pf1N1B4_6040 at 503.949 kb on - strand, within Pf1N1B4_6040 at 503.949 kb on - strand, within Pf1N1B4_6040 at 504.115 kb on - strand at 504.115 kb on - strand at 504.251 kb on - strand at 504.251 kb on - strand at 504.252 kb on + strand at 504.252 kb on + strand at 504.252 kb on + strand at 504.252 kb on + strand at 504.253 kb on - strand at 504.253 kb on - strand at 504.253 kb on - strand at 504.253 kb on - strand at 504.253 kb on - strand at 504.253 kb on - strand at 504.253 kb on - strand at 504.253 kb on - strand at 504.257 kb on - strand at 504.271 kb on - strand at 504.271 kb on - strand at 504.324 kb on - strand at 504.324 kb on - strand at 504.328 kb on + strand at 504.328 kb on + strand at 504.328 kb on + strand at 504.328 kb on + strand at 504.328 kb on + strand at 504.328 kb on + strand at 504.328 kb on + strand at 504.328 kb on + strand at 504.329 kb on - strand at 504.329 kb on - strand at 504.329 kb on - strand at 504.329 kb on - strand at 504.329 kb on - strand at 504.329 kb on - strand at 504.331 kb on - strand at 504.331 kb on - strand at 504.334 kb on + strand at 504.334 kb on + strand at 504.334 kb on + strand at 504.335 kb on - strand at 504.335 kb on - strand at 504.335 kb on - strand at 504.335 kb on - strand at 504.335 kb on - strand at 504.335 kb on - strand at 504.335 kb on - strand at 504.335 kb on - strand at 504.369 kb on + strand at 504.370 kb on - strand at 504.400 kb on + strand at 504.427 kb on + strand, within Pf1N1B4_6041 at 504.428 kb on - strand, within Pf1N1B4_6041 at 504.446 kb on + strand, within Pf1N1B4_6041 at 504.447 kb on - strand, within Pf1N1B4_6041 at 504.711 kb on - strand at 504.854 kb on - strand, within Pf1N1B4_6042 at 504.872 kb on + strand at 504.873 kb on - strand, within Pf1N1B4_6042 at 504.873 kb on - strand, within Pf1N1B4_6042 at 504.873 kb on - strand, within Pf1N1B4_6042 at 505.015 kb on - strand, within Pf1N1B4_6042 at 505.015 kb on - strand, within Pf1N1B4_6042 at 505.018 kb on - strand, within Pf1N1B4_6042 at 505.018 kb on - strand, within Pf1N1B4_6042 at 505.018 kb on - strand, within Pf1N1B4_6042 at 505.152 kb on - strand, within Pf1N1B4_6042 at 505.152 kb on - strand, within Pf1N1B4_6042 at 505.244 kb on - strand at 505.244 kb on - strand at 505.244 kb on - strand at 505.244 kb on - strand at 505.244 kb on - strand at 505.244 kb on - strand at 505.251 kb on - strand at 505.273 kb on + strand at 505.273 kb on + strand at 505.274 kb on - strand at 505.274 kb on - strand at 505.274 kb on - strand at 505.274 kb on - strand at 505.278 kb on + strand at 505.281 kb on + strand at 505.281 kb on + strand at 505.282 kb on - strand at 505.282 kb on - strand at 505.282 kb on - strand at 505.282 kb on - strand at 505.282 kb on - strand at 505.282 kb on - strand at 505.297 kb on - strand at 505.297 kb on - strand at 505.297 kb on - strand at 505.297 kb on - strand at 505.363 kb on + strand at 505.364 kb on - strand at 505.364 kb on - strand at 505.378 kb on - strand at 505.378 kb on - strand at 505.378 kb on - strand at 505.378 kb on - strand at 505.580 kb on - strand, within Pf1N1B4_6043 at 505.616 kb on - strand, within Pf1N1B4_6043 at 505.637 kb on - strand, within Pf1N1B4_6043 at 505.637 kb on - strand, within Pf1N1B4_6043 at 505.714 kb on + strand at 505.729 kb on + strand, within Pf1N1B4_6043 at 505.730 kb on - strand, within Pf1N1B4_6043 at 505.730 kb on - strand, within Pf1N1B4_6043 at 505.735 kb on + strand, within Pf1N1B4_6043 at 505.736 kb on - strand, within Pf1N1B4_6043 at 505.736 kb on - strand at 505.753 kb on + strand, within Pf1N1B4_6043 at 506.083 kb on + strand, within Pf1N1B4_6043 at 506.084 kb on - strand, within Pf1N1B4_6043 at 506.084 kb on - strand, within Pf1N1B4_6043 at 506.104 kb on + strand, within Pf1N1B4_6043 at 506.104 kb on + strand, within Pf1N1B4_6043 at 506.104 kb on + strand, within Pf1N1B4_6043 at 506.105 kb on - strand, within Pf1N1B4_6043 at 506.105 kb on - strand, within Pf1N1B4_6043 at 506.186 kb on + strand at 506.206 kb on - strand, within Pf1N1B4_6043 at 506.206 kb on - strand, within Pf1N1B4_6043 at 506.206 kb on - strand, within Pf1N1B4_6043 at 506.206 kb on - strand, within Pf1N1B4_6043
Per-strain Table
Position Strand Gene LocusTag Fraction Cholin acetate 10 mM remove 503,889 - Pf1N1B4_6040 0.30 -0.4 503,889 - Pf1N1B4_6040 0.30 +1.4 503,948 + -0.6 503,949 - Pf1N1B4_6040 0.47 +1.0 503,949 - +1.6 503,949 - Pf1N1B4_6040 0.47 -0.4 503,949 - Pf1N1B4_6040 0.47 +0.1 503,949 - Pf1N1B4_6040 0.47 -1.4 503,949 - Pf1N1B4_6040 0.47 +0.3 503,949 - Pf1N1B4_6040 0.47 +1.1 503,949 - Pf1N1B4_6040 0.47 +0.6 503,949 - Pf1N1B4_6040 0.47 -0.3 503,949 - Pf1N1B4_6040 0.47 +0.6 504,115 - -2.2 504,115 - -0.7 504,251 - -0.5 504,251 - -1.4 504,252 + -1.0 504,252 + +0.6 504,252 + +0.2 504,252 + -1.6 504,253 - -0.2 504,253 - -0.4 504,253 - -2.0 504,253 - -0.1 504,253 - -1.4 504,253 - -0.4 504,253 - -0.4 504,253 - +1.0 504,257 - -2.2 504,271 - +0.6 504,271 - -1.3 504,324 - +0.6 504,324 - +0.6 504,328 + +0.6 504,328 + +0.0 504,328 + -0.7 504,328 + -0.8 504,328 + +0.8 504,328 + +0.6 504,328 + +0.1 504,328 + +0.0 504,329 - -1.4 504,329 - -1.7 504,329 - +0.6 504,329 - +1.6 504,329 - -0.4 504,329 - -1.0 504,331 - -0.1 504,331 - -1.1 504,334 + +1.2 504,334 + -0.8 504,334 + +0.1 504,335 - +0.2 504,335 - -1.0 504,335 - +0.6 504,335 - -1.7 504,335 - -0.4 504,335 - -2.1 504,335 - +0.1 504,335 - -0.0 504,369 + -0.4 504,370 - -1.1 504,400 + -0.7 504,427 + Pf1N1B4_6041 0.17 -1.2 504,428 - Pf1N1B4_6041 0.18 +0.4 504,446 + Pf1N1B4_6041 0.27 -2.0 504,447 - Pf1N1B4_6041 0.28 +1.6 504,711 - -0.4 504,854 - Pf1N1B4_6042 0.16 +0.1 504,872 + -2.0 504,873 - Pf1N1B4_6042 0.20 +0.6 504,873 - Pf1N1B4_6042 0.20 -0.7 504,873 - Pf1N1B4_6042 0.20 -1.7 505,015 - Pf1N1B4_6042 0.51 -0.4 505,015 - Pf1N1B4_6042 0.51 -0.9 505,018 - Pf1N1B4_6042 0.52 +0.1 505,018 - Pf1N1B4_6042 0.52 -1.0 505,018 - Pf1N1B4_6042 0.52 -0.4 505,152 - Pf1N1B4_6042 0.81 -0.7 505,152 - Pf1N1B4_6042 0.81 -0.4 505,244 - +0.6 505,244 - +2.2 505,244 - -1.0 505,244 - -0.6 505,244 - +0.8 505,244 - +4.1 505,251 - +3.2 505,273 + -1.0 505,273 + -0.3 505,274 - -0.4 505,274 - +2.6 505,274 - +0.2 505,274 - +0.2 505,278 + -1.0 505,281 + -1.7 505,281 + -0.1 505,282 - +0.4 505,282 - -1.0 505,282 - +0.6 505,282 - -0.4 505,282 - +0.6 505,282 - -1.0 505,297 - -0.2 505,297 - -2.0 505,297 - -0.5 505,297 - +0.0 505,363 + +0.0 505,364 - +0.6 505,364 - +1.9 505,378 - -2.2 505,378 - -1.7 505,378 - +0.0 505,378 - -0.0 505,580 - Pf1N1B4_6043 0.19 +0.2 505,616 - Pf1N1B4_6043 0.22 +0.4 505,637 - Pf1N1B4_6043 0.24 -0.5 505,637 - Pf1N1B4_6043 0.24 +0.2 505,714 + -0.4 505,729 + Pf1N1B4_6043 0.31 -1.0 505,730 - Pf1N1B4_6043 0.31 +0.2 505,730 - Pf1N1B4_6043 0.31 -1.7 505,735 + Pf1N1B4_6043 0.32 -0.5 505,736 - Pf1N1B4_6043 0.32 +0.2 505,736 - +0.3 505,753 + Pf1N1B4_6043 0.33 -1.7 506,083 + Pf1N1B4_6043 0.60 +0.9 506,084 - Pf1N1B4_6043 0.60 +0.0 506,084 - Pf1N1B4_6043 0.60 -0.0 506,104 + Pf1N1B4_6043 0.62 -1.0 506,104 + Pf1N1B4_6043 0.62 +0.5 506,104 + Pf1N1B4_6043 0.62 +1.6 506,105 - Pf1N1B4_6043 0.62 -0.4 506,105 - Pf1N1B4_6043 0.62 +0.3 506,186 + +0.2 506,206 - Pf1N1B4_6043 0.70 -0.4 506,206 - Pf1N1B4_6043 0.70 +0.6 506,206 - Pf1N1B4_6043 0.70 -1.7 506,206 - Pf1N1B4_6043 0.70 -0.6
Or see this region's nucleotide sequence