Experiment: Cholin acetate 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5910 and Pf1N1B4_5911 overlap by 8 nucleotides Pf1N1B4_5911 and Pf1N1B4_5912 are separated by 275 nucleotides Pf1N1B4_5912 and Pf1N1B4_5913 are separated by 151 nucleotides Pf1N1B4_5913 and Pf1N1B4_5914 are separated by 210 nucleotides
Pf1N1B4_5910 - putative membrane protein, at 353,971 to 354,561
_5910
Pf1N1B4_5911 - DoxX family protein, at 354,554 to 354,970
_5911
Pf1N1B4_5912 - Tryptophan synthase beta chain like (EC 4.2.1.20), at 355,246 to 355,614
_5912
Pf1N1B4_5913 - hypothetical protein, at 355,766 to 355,930
_5913
Pf1N1B4_5914 - Transcriptional regulator, LysR family, at 356,141 to 357,064
_5914
Position (kb) 355
356 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 354.801 kb on - strand at 354.858 kb on + strand, within Pf1N1B4_5911 at 354.858 kb on + strand at 355.036 kb on + strand at 355.036 kb on + strand at 355.037 kb on - strand at 355.037 kb on - strand at 355.037 kb on - strand at 355.341 kb on + strand, within Pf1N1B4_5912 at 355.342 kb on - strand, within Pf1N1B4_5912 at 355.342 kb on - strand, within Pf1N1B4_5912 at 355.427 kb on - strand, within Pf1N1B4_5912 at 355.427 kb on - strand, within Pf1N1B4_5912 at 355.460 kb on + strand at 355.565 kb on + strand, within Pf1N1B4_5912 at 355.565 kb on + strand, within Pf1N1B4_5912 at 355.631 kb on - strand at 355.631 kb on - strand at 355.668 kb on - strand at 355.668 kb on - strand at 355.706 kb on + strand at 355.706 kb on + strand at 355.706 kb on + strand at 355.707 kb on - strand at 355.707 kb on - strand at 355.707 kb on - strand at 355.708 kb on + strand at 355.708 kb on + strand at 355.708 kb on + strand at 355.708 kb on + strand at 355.781 kb on + strand at 355.782 kb on - strand at 355.803 kb on + strand, within Pf1N1B4_5913 at 355.805 kb on + strand, within Pf1N1B4_5913 at 355.806 kb on - strand at 355.928 kb on + strand at 355.929 kb on - strand at 355.929 kb on - strand at 355.929 kb on - strand at 355.943 kb on - strand at 355.943 kb on - strand at 355.958 kb on + strand at 356.293 kb on + strand, within Pf1N1B4_5914 at 356.293 kb on + strand, within Pf1N1B4_5914 at 356.293 kb on + strand, within Pf1N1B4_5914 at 356.293 kb on + strand, within Pf1N1B4_5914 at 356.293 kb on + strand, within Pf1N1B4_5914 at 356.322 kb on + strand, within Pf1N1B4_5914 at 356.598 kb on + strand, within Pf1N1B4_5914
Per-strain Table
Position Strand Gene LocusTag Fraction Cholin acetate 10 mM remove 354,801 - +0.6 354,858 + Pf1N1B4_5911 0.73 +0.2 354,858 + -0.4 355,036 + -1.7 355,036 + +1.7 355,037 - -2.0 355,037 - -0.1 355,037 - -1.7 355,341 + Pf1N1B4_5912 0.26 +1.2 355,342 - Pf1N1B4_5912 0.26 -0.4 355,342 - Pf1N1B4_5912 0.26 -1.4 355,427 - Pf1N1B4_5912 0.49 -0.7 355,427 - Pf1N1B4_5912 0.49 +0.6 355,460 + -1.4 355,565 + Pf1N1B4_5912 0.86 -1.0 355,565 + Pf1N1B4_5912 0.86 -1.4 355,631 - +1.6 355,631 - +1.6 355,668 - -1.3 355,668 - +0.6 355,706 + +0.2 355,706 + -0.1 355,706 + -0.6 355,707 - -1.7 355,707 - +0.9 355,707 - +0.2 355,708 + -1.4 355,708 + +0.2 355,708 + +1.2 355,708 + +0.8 355,781 + +0.6 355,782 - +1.2 355,803 + Pf1N1B4_5913 0.22 -0.6 355,805 + Pf1N1B4_5913 0.24 +0.6 355,806 - +0.6 355,928 + +0.0 355,929 - -0.8 355,929 - -1.4 355,929 - -0.6 355,943 - +3.9 355,943 - -0.6 355,958 + +1.3 356,293 + Pf1N1B4_5914 0.16 +0.4 356,293 + Pf1N1B4_5914 0.16 +1.0 356,293 + Pf1N1B4_5914 0.16 -0.0 356,293 + Pf1N1B4_5914 0.16 +0.6 356,293 + Pf1N1B4_5914 0.16 +1.4 356,322 + Pf1N1B4_5914 0.20 -0.9 356,598 + Pf1N1B4_5914 0.49 +0.0
Or see this region's nucleotide sequence