Experiment: Cholin acetate 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5877 and Pf1N1B4_5878 are separated by 86 nucleotides Pf1N1B4_5878 and Pf1N1B4_5879 are separated by 10 nucleotides Pf1N1B4_5879 and Pf1N1B4_5880 are separated by 163 nucleotides Pf1N1B4_5880 and Pf1N1B4_5881 overlap by 11 nucleotides
Pf1N1B4_5877 - FIG00967533: hypothetical protein, at 318,840 to 319,424
_5877
Pf1N1B4_5878 - hypothetical protein, at 319,511 to 319,684
_5878
Pf1N1B4_5879 - hypothetical protein, at 319,695 to 319,937
_5879
Pf1N1B4_5880 - AttH component of AttEFGH ABC transport system, at 320,101 to 321,174
_5880
Pf1N1B4_5881 - AttF component of AttEFGH ABC transport system / AttG component of AttEFGH ABC transport system, at 321,164 to 323,635
_5881
Position (kb)
320
321
322 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 319.144 kb on + strand, within Pf1N1B4_5877 at 319.230 kb on + strand, within Pf1N1B4_5877 at 319.230 kb on + strand, within Pf1N1B4_5877 at 319.230 kb on + strand, within Pf1N1B4_5877 at 319.231 kb on - strand, within Pf1N1B4_5877 at 319.424 kb on + strand at 319.425 kb on - strand at 319.431 kb on + strand at 319.467 kb on + strand at 319.501 kb on + strand at 319.502 kb on - strand at 319.502 kb on - strand at 319.502 kb on - strand at 319.833 kb on + strand, within Pf1N1B4_5879 at 319.957 kb on + strand at 319.958 kb on - strand at 320.010 kb on - strand at 320.059 kb on - strand at 320.079 kb on + strand at 320.079 kb on + strand at 320.080 kb on - strand at 320.102 kb on + strand at 320.103 kb on - strand at 320.103 kb on - strand at 320.124 kb on - strand at 320.372 kb on + strand, within Pf1N1B4_5880 at 320.522 kb on + strand, within Pf1N1B4_5880 at 320.523 kb on - strand at 320.576 kb on + strand, within Pf1N1B4_5880 at 320.984 kb on + strand, within Pf1N1B4_5880 at 320.984 kb on + strand at 320.984 kb on + strand, within Pf1N1B4_5880 at 321.063 kb on + strand at 321.165 kb on + strand at 321.296 kb on + strand at 321.453 kb on - strand, within Pf1N1B4_5881 at 321.975 kb on + strand, within Pf1N1B4_5881 at 321.975 kb on + strand, within Pf1N1B4_5881 at 321.976 kb on - strand, within Pf1N1B4_5881 at 322.128 kb on + strand, within Pf1N1B4_5881
Per-strain Table
Position Strand Gene LocusTag Fraction Cholin acetate 10 mM remove 319,144 + Pf1N1B4_5877 0.52 -0.4 319,230 + Pf1N1B4_5877 0.67 -0.3 319,230 + Pf1N1B4_5877 0.67 +1.1 319,230 + Pf1N1B4_5877 0.67 -0.1 319,231 - Pf1N1B4_5877 0.67 -0.1 319,424 + +1.2 319,425 - -0.1 319,431 + -0.6 319,467 + +0.6 319,501 + +0.6 319,502 - +1.0 319,502 - +0.2 319,502 - -1.0 319,833 + Pf1N1B4_5879 0.57 +0.0 319,957 + -0.6 319,958 - -1.4 320,010 - -0.9 320,059 - -0.4 320,079 + -0.4 320,079 + +2.6 320,080 - +3.2 320,102 + +1.2 320,103 - +0.3 320,103 - -1.4 320,124 - -0.6 320,372 + Pf1N1B4_5880 0.25 -1.4 320,522 + Pf1N1B4_5880 0.39 -0.0 320,523 - +0.6 320,576 + Pf1N1B4_5880 0.44 +0.3 320,984 + Pf1N1B4_5880 0.82 -1.1 320,984 + +2.9 320,984 + Pf1N1B4_5880 0.82 -2.0 321,063 + -0.4 321,165 + -0.7 321,296 + +0.5 321,453 - Pf1N1B4_5881 0.12 +0.0 321,975 + Pf1N1B4_5881 0.33 +0.6 321,975 + Pf1N1B4_5881 0.33 +0.6 321,976 - Pf1N1B4_5881 0.33 +1.3 322,128 + Pf1N1B4_5881 0.39 -0.1
Or see this region's nucleotide sequence