Experiment: Cholin acetate 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5862 and Pf1N1B4_5863 overlap by 1 nucleotides Pf1N1B4_5863 and Pf1N1B4_5864 are separated by 1 nucleotides
Pf1N1B4_5862 - Glycolate dehydrogenase (EC 1.1.99.14), subunit GlcD, at 296,341 to 297,840
_5862
Pf1N1B4_5863 - Glycolate dehydrogenase (EC 1.1.99.14), FAD-binding subunit GlcE, at 297,840 to 298,889
_5863
Pf1N1B4_5864 - Glycolate dehydrogenase (EC 1.1.99.14), iron-sulfur subunit GlcF, at 298,891 to 300,108
_5864
Position (kb) 297
298
299 Strain fitness (log2 ratio) -2
-1
0
1
2 at 296.854 kb on + strand, within Pf1N1B4_5862 at 296.867 kb on + strand, within Pf1N1B4_5862 at 296.868 kb on - strand, within Pf1N1B4_5862 at 297.395 kb on - strand, within Pf1N1B4_5862 at 297.513 kb on + strand, within Pf1N1B4_5862 at 297.514 kb on - strand, within Pf1N1B4_5862 at 298.003 kb on - strand, within Pf1N1B4_5863 at 298.003 kb on - strand, within Pf1N1B4_5863 at 298.306 kb on - strand, within Pf1N1B4_5863 at 298.554 kb on + strand at 298.583 kb on - strand, within Pf1N1B4_5863 at 298.583 kb on - strand, within Pf1N1B4_5863 at 298.887 kb on + strand at 298.887 kb on + strand at 298.888 kb on - strand at 298.890 kb on + strand at 298.891 kb on - strand at 299.002 kb on + strand at 299.003 kb on - strand at 299.047 kb on + strand, within Pf1N1B4_5864 at 299.047 kb on + strand, within Pf1N1B4_5864 at 299.048 kb on - strand, within Pf1N1B4_5864 at 299.531 kb on - strand, within Pf1N1B4_5864 at 299.692 kb on + strand, within Pf1N1B4_5864 at 299.693 kb on - strand, within Pf1N1B4_5864 at 299.814 kb on + strand at 299.849 kb on + strand, within Pf1N1B4_5864
Per-strain Table
Position Strand Gene LocusTag Fraction Cholin acetate 10 mM remove 296,854 + Pf1N1B4_5862 0.34 -0.1 296,867 + Pf1N1B4_5862 0.35 +2.2 296,868 - Pf1N1B4_5862 0.35 +0.3 297,395 - Pf1N1B4_5862 0.70 +0.3 297,513 + Pf1N1B4_5862 0.78 -0.1 297,514 - Pf1N1B4_5862 0.78 -0.3 298,003 - Pf1N1B4_5863 0.16 +0.6 298,003 - Pf1N1B4_5863 0.16 -0.4 298,306 - Pf1N1B4_5863 0.44 -1.2 298,554 + +0.6 298,583 - Pf1N1B4_5863 0.71 -0.7 298,583 - Pf1N1B4_5863 0.71 -0.4 298,887 + -0.3 298,887 + +0.6 298,888 - -1.1 298,890 + +0.3 298,891 - -1.0 299,002 + +0.1 299,003 - +2.3 299,047 + Pf1N1B4_5864 0.13 -2.0 299,047 + Pf1N1B4_5864 0.13 +2.6 299,048 - Pf1N1B4_5864 0.13 +2.2 299,531 - Pf1N1B4_5864 0.53 +1.0 299,692 + Pf1N1B4_5864 0.66 -1.4 299,693 - Pf1N1B4_5864 0.66 +0.4 299,814 + -0.4 299,849 + Pf1N1B4_5864 0.79 +0.6
Or see this region's nucleotide sequence