Experiment: Cholin acetate 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4480 and Pf1N1B4_4481 are separated by 185 nucleotides Pf1N1B4_4481 and Pf1N1B4_4482 are separated by 128 nucleotides Pf1N1B4_4482 and Pf1N1B4_4483 are separated by 104 nucleotides Pf1N1B4_4483 and Pf1N1B4_4484 are separated by 183 nucleotides
Pf1N1B4_4480 - Branched-chain alpha-keto acid dehydrogenase, E1 component, alpha subunit (EC 1.2.4.4), at 4,941,511 to 4,942,746
_4480
Pf1N1B4_4481 - Transcriptional regulator BkdR of isoleucine and valine catabolism operon, at 4,942,932 to 4,943,420
_4481
Pf1N1B4_4482 - FIG146518: Zn-dependent hydrolases, including glyoxylases, at 4,943,549 to 4,944,412
_4482
Pf1N1B4_4483 - Transcriptional regulator, ArsR family, at 4,944,517 to 4,944,852
_4483
Pf1N1B4_4484 - FIG002984: FAD-dependent pyridine nucleotide-disulphide oxidoreductase, at 4,945,036 to 4,946,280
_4484
Position (kb)
4943
4944
4945 Strain fitness (log2 ratio)
-2
-1
0
1 at 4942.668 kb on - strand at 4942.753 kb on + strand at 4942.753 kb on + strand at 4942.753 kb on + strand at 4942.779 kb on - strand at 4942.779 kb on - strand at 4942.851 kb on - strand at 4942.904 kb on - strand at 4943.226 kb on + strand, within Pf1N1B4_4481 at 4943.251 kb on - strand, within Pf1N1B4_4481 at 4943.251 kb on - strand at 4943.370 kb on + strand, within Pf1N1B4_4481 at 4943.370 kb on + strand, within Pf1N1B4_4481 at 4943.370 kb on + strand, within Pf1N1B4_4481 at 4943.372 kb on + strand at 4943.372 kb on + strand at 4943.372 kb on + strand at 4943.372 kb on + strand at 4943.550 kb on + strand at 4943.550 kb on + strand at 4943.578 kb on - strand at 4943.809 kb on - strand, within Pf1N1B4_4482 at 4943.809 kb on - strand, within Pf1N1B4_4482 at 4943.809 kb on - strand, within Pf1N1B4_4482 at 4943.809 kb on - strand, within Pf1N1B4_4482 at 4943.958 kb on + strand, within Pf1N1B4_4482 at 4943.959 kb on - strand, within Pf1N1B4_4482 at 4943.959 kb on - strand, within Pf1N1B4_4482 at 4943.959 kb on - strand, within Pf1N1B4_4482 at 4943.978 kb on - strand, within Pf1N1B4_4482 at 4944.303 kb on + strand, within Pf1N1B4_4482 at 4944.351 kb on + strand at 4944.352 kb on - strand at 4944.360 kb on + strand at 4944.360 kb on + strand at 4944.360 kb on + strand at 4944.360 kb on + strand at 4944.361 kb on - strand at 4944.366 kb on + strand at 4944.367 kb on - strand at 4944.367 kb on - strand at 4944.465 kb on + strand at 4944.465 kb on + strand at 4944.466 kb on - strand at 4944.735 kb on - strand, within Pf1N1B4_4483 at 4944.757 kb on + strand, within Pf1N1B4_4483 at 4944.757 kb on + strand, within Pf1N1B4_4483 at 4944.757 kb on + strand, within Pf1N1B4_4483 at 4944.758 kb on - strand, within Pf1N1B4_4483 at 4944.758 kb on - strand at 4944.758 kb on - strand, within Pf1N1B4_4483 at 4944.761 kb on + strand, within Pf1N1B4_4483 at 4944.761 kb on + strand, within Pf1N1B4_4483 at 4944.761 kb on + strand, within Pf1N1B4_4483 at 4944.761 kb on + strand, within Pf1N1B4_4483 at 4944.761 kb on + strand, within Pf1N1B4_4483 at 4944.761 kb on + strand, within Pf1N1B4_4483 at 4944.761 kb on + strand, within Pf1N1B4_4483 at 4944.762 kb on - strand, within Pf1N1B4_4483 at 4944.766 kb on + strand, within Pf1N1B4_4483 at 4944.766 kb on + strand, within Pf1N1B4_4483 at 4944.767 kb on - strand, within Pf1N1B4_4483 at 4944.820 kb on + strand at 4944.821 kb on - strand at 4944.977 kb on + strand at 4945.021 kb on - strand at 4945.083 kb on - strand at 4945.085 kb on + strand at 4945.086 kb on - strand at 4945.180 kb on + strand, within Pf1N1B4_4484 at 4945.180 kb on + strand, within Pf1N1B4_4484 at 4945.180 kb on + strand, within Pf1N1B4_4484 at 4945.184 kb on - strand, within Pf1N1B4_4484 at 4945.220 kb on - strand, within Pf1N1B4_4484 at 4945.220 kb on - strand, within Pf1N1B4_4484
Per-strain Table
Position Strand Gene LocusTag Fraction Cholin acetate 10 mM remove 4,942,668 - -0.6 4,942,753 + +0.6 4,942,753 + +1.2 4,942,753 + -0.6 4,942,779 - -0.4 4,942,779 - +0.1 4,942,851 - +0.0 4,942,904 - +0.2 4,943,226 + Pf1N1B4_4481 0.60 -0.4 4,943,251 - Pf1N1B4_4481 0.65 +0.0 4,943,251 - -0.4 4,943,370 + Pf1N1B4_4481 0.90 +0.6 4,943,370 + Pf1N1B4_4481 0.90 -0.1 4,943,370 + Pf1N1B4_4481 0.90 -1.0 4,943,372 + -1.6 4,943,372 + +0.2 4,943,372 + -0.2 4,943,372 + +0.1 4,943,550 + -0.4 4,943,550 + +0.0 4,943,578 - +0.2 4,943,809 - Pf1N1B4_4482 0.30 -1.0 4,943,809 - Pf1N1B4_4482 0.30 -1.7 4,943,809 - Pf1N1B4_4482 0.30 -1.7 4,943,809 - Pf1N1B4_4482 0.30 +0.3 4,943,958 + Pf1N1B4_4482 0.47 +0.3 4,943,959 - Pf1N1B4_4482 0.47 -1.4 4,943,959 - Pf1N1B4_4482 0.47 -1.0 4,943,959 - Pf1N1B4_4482 0.47 +0.6 4,943,978 - Pf1N1B4_4482 0.50 -0.4 4,944,303 + Pf1N1B4_4482 0.87 -0.2 4,944,351 + +0.2 4,944,352 - -0.4 4,944,360 + -2.2 4,944,360 + +0.2 4,944,360 + +0.6 4,944,360 + -0.1 4,944,361 - +0.6 4,944,366 + +0.6 4,944,367 - -1.0 4,944,367 - +0.2 4,944,465 + +0.6 4,944,465 + +0.3 4,944,466 - +0.2 4,944,735 - Pf1N1B4_4483 0.65 +0.6 4,944,757 + Pf1N1B4_4483 0.71 -0.4 4,944,757 + Pf1N1B4_4483 0.71 +0.1 4,944,757 + Pf1N1B4_4483 0.71 +0.6 4,944,758 - Pf1N1B4_4483 0.72 -0.7 4,944,758 - -0.0 4,944,758 - Pf1N1B4_4483 0.72 -1.4 4,944,761 + Pf1N1B4_4483 0.73 -1.4 4,944,761 + Pf1N1B4_4483 0.73 +0.3 4,944,761 + Pf1N1B4_4483 0.73 +0.9 4,944,761 + Pf1N1B4_4483 0.73 +0.6 4,944,761 + Pf1N1B4_4483 0.73 -0.0 4,944,761 + Pf1N1B4_4483 0.73 -0.9 4,944,761 + Pf1N1B4_4483 0.73 -0.3 4,944,762 - Pf1N1B4_4483 0.73 +0.2 4,944,766 + Pf1N1B4_4483 0.74 +1.6 4,944,766 + Pf1N1B4_4483 0.74 -0.1 4,944,767 - Pf1N1B4_4483 0.74 +0.8 4,944,820 + +0.3 4,944,821 - +0.9 4,944,977 + -0.4 4,945,021 - -1.6 4,945,083 - -0.2 4,945,085 + -0.3 4,945,086 - -0.1 4,945,180 + Pf1N1B4_4484 0.12 +0.7 4,945,180 + Pf1N1B4_4484 0.12 -2.2 4,945,180 + Pf1N1B4_4484 0.12 +1.2 4,945,184 - Pf1N1B4_4484 0.12 -0.4 4,945,220 - Pf1N1B4_4484 0.15 -1.4 4,945,220 - Pf1N1B4_4484 0.15 +1.1
Or see this region's nucleotide sequence