Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_4482

Experiment: Cholin acetate 10 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_4480 and Pf1N1B4_4481 are separated by 185 nucleotidesPf1N1B4_4481 and Pf1N1B4_4482 are separated by 128 nucleotidesPf1N1B4_4482 and Pf1N1B4_4483 are separated by 104 nucleotidesPf1N1B4_4483 and Pf1N1B4_4484 are separated by 183 nucleotides Pf1N1B4_4480 - Branched-chain alpha-keto acid dehydrogenase, E1 component, alpha subunit (EC 1.2.4.4), at 4,941,511 to 4,942,746 _4480 Pf1N1B4_4481 - Transcriptional regulator BkdR of isoleucine and valine catabolism operon, at 4,942,932 to 4,943,420 _4481 Pf1N1B4_4482 - FIG146518: Zn-dependent hydrolases, including glyoxylases, at 4,943,549 to 4,944,412 _4482 Pf1N1B4_4483 - Transcriptional regulator, ArsR family, at 4,944,517 to 4,944,852 _4483 Pf1N1B4_4484 - FIG002984: FAD-dependent pyridine nucleotide-disulphide oxidoreductase, at 4,945,036 to 4,946,280 _4484 Position (kb) 4943 4944 4945Strain fitness (log2 ratio) -2 -1 0 1at 4942.668 kb on - strandat 4942.753 kb on + strandat 4942.753 kb on + strandat 4942.753 kb on + strandat 4942.779 kb on - strandat 4942.779 kb on - strandat 4942.851 kb on - strandat 4942.904 kb on - strandat 4943.226 kb on + strand, within Pf1N1B4_4481at 4943.251 kb on - strand, within Pf1N1B4_4481at 4943.251 kb on - strandat 4943.370 kb on + strand, within Pf1N1B4_4481at 4943.370 kb on + strand, within Pf1N1B4_4481at 4943.370 kb on + strand, within Pf1N1B4_4481at 4943.372 kb on + strandat 4943.372 kb on + strandat 4943.372 kb on + strandat 4943.372 kb on + strandat 4943.550 kb on + strandat 4943.550 kb on + strandat 4943.578 kb on - strandat 4943.809 kb on - strand, within Pf1N1B4_4482at 4943.809 kb on - strand, within Pf1N1B4_4482at 4943.809 kb on - strand, within Pf1N1B4_4482at 4943.809 kb on - strand, within Pf1N1B4_4482at 4943.958 kb on + strand, within Pf1N1B4_4482at 4943.959 kb on - strand, within Pf1N1B4_4482at 4943.959 kb on - strand, within Pf1N1B4_4482at 4943.959 kb on - strand, within Pf1N1B4_4482at 4943.978 kb on - strand, within Pf1N1B4_4482at 4944.303 kb on + strand, within Pf1N1B4_4482at 4944.351 kb on + strandat 4944.352 kb on - strandat 4944.360 kb on + strandat 4944.360 kb on + strandat 4944.360 kb on + strandat 4944.360 kb on + strandat 4944.361 kb on - strandat 4944.366 kb on + strandat 4944.367 kb on - strandat 4944.367 kb on - strandat 4944.465 kb on + strandat 4944.465 kb on + strandat 4944.466 kb on - strandat 4944.735 kb on - strand, within Pf1N1B4_4483at 4944.757 kb on + strand, within Pf1N1B4_4483at 4944.757 kb on + strand, within Pf1N1B4_4483at 4944.757 kb on + strand, within Pf1N1B4_4483at 4944.758 kb on - strand, within Pf1N1B4_4483at 4944.758 kb on - strandat 4944.758 kb on - strand, within Pf1N1B4_4483at 4944.761 kb on + strand, within Pf1N1B4_4483at 4944.761 kb on + strand, within Pf1N1B4_4483at 4944.761 kb on + strand, within Pf1N1B4_4483at 4944.761 kb on + strand, within Pf1N1B4_4483at 4944.761 kb on + strand, within Pf1N1B4_4483at 4944.761 kb on + strand, within Pf1N1B4_4483at 4944.761 kb on + strand, within Pf1N1B4_4483at 4944.762 kb on - strand, within Pf1N1B4_4483at 4944.766 kb on + strand, within Pf1N1B4_4483at 4944.766 kb on + strand, within Pf1N1B4_4483at 4944.767 kb on - strand, within Pf1N1B4_4483at 4944.820 kb on + strandat 4944.821 kb on - strandat 4944.977 kb on + strandat 4945.021 kb on - strandat 4945.083 kb on - strandat 4945.085 kb on + strandat 4945.086 kb on - strandat 4945.180 kb on + strand, within Pf1N1B4_4484at 4945.180 kb on + strand, within Pf1N1B4_4484at 4945.180 kb on + strand, within Pf1N1B4_4484at 4945.184 kb on - strand, within Pf1N1B4_4484at 4945.220 kb on - strand, within Pf1N1B4_4484at 4945.220 kb on - strand, within Pf1N1B4_4484

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Per-strain Table

Position Strand Gene LocusTag Fraction Cholin acetate 10 mM
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4,942,668 - -0.6
4,942,753 + +0.6
4,942,753 + +1.2
4,942,753 + -0.6
4,942,779 - -0.4
4,942,779 - +0.1
4,942,851 - +0.0
4,942,904 - +0.2
4,943,226 + Pf1N1B4_4481 0.60 -0.4
4,943,251 - Pf1N1B4_4481 0.65 +0.0
4,943,251 - -0.4
4,943,370 + Pf1N1B4_4481 0.90 +0.6
4,943,370 + Pf1N1B4_4481 0.90 -0.1
4,943,370 + Pf1N1B4_4481 0.90 -1.0
4,943,372 + -1.6
4,943,372 + +0.2
4,943,372 + -0.2
4,943,372 + +0.1
4,943,550 + -0.4
4,943,550 + +0.0
4,943,578 - +0.2
4,943,809 - Pf1N1B4_4482 0.30 -1.0
4,943,809 - Pf1N1B4_4482 0.30 -1.7
4,943,809 - Pf1N1B4_4482 0.30 -1.7
4,943,809 - Pf1N1B4_4482 0.30 +0.3
4,943,958 + Pf1N1B4_4482 0.47 +0.3
4,943,959 - Pf1N1B4_4482 0.47 -1.4
4,943,959 - Pf1N1B4_4482 0.47 -1.0
4,943,959 - Pf1N1B4_4482 0.47 +0.6
4,943,978 - Pf1N1B4_4482 0.50 -0.4
4,944,303 + Pf1N1B4_4482 0.87 -0.2
4,944,351 + +0.2
4,944,352 - -0.4
4,944,360 + -2.2
4,944,360 + +0.2
4,944,360 + +0.6
4,944,360 + -0.1
4,944,361 - +0.6
4,944,366 + +0.6
4,944,367 - -1.0
4,944,367 - +0.2
4,944,465 + +0.6
4,944,465 + +0.3
4,944,466 - +0.2
4,944,735 - Pf1N1B4_4483 0.65 +0.6
4,944,757 + Pf1N1B4_4483 0.71 -0.4
4,944,757 + Pf1N1B4_4483 0.71 +0.1
4,944,757 + Pf1N1B4_4483 0.71 +0.6
4,944,758 - Pf1N1B4_4483 0.72 -0.7
4,944,758 - -0.0
4,944,758 - Pf1N1B4_4483 0.72 -1.4
4,944,761 + Pf1N1B4_4483 0.73 -1.4
4,944,761 + Pf1N1B4_4483 0.73 +0.3
4,944,761 + Pf1N1B4_4483 0.73 +0.9
4,944,761 + Pf1N1B4_4483 0.73 +0.6
4,944,761 + Pf1N1B4_4483 0.73 -0.0
4,944,761 + Pf1N1B4_4483 0.73 -0.9
4,944,761 + Pf1N1B4_4483 0.73 -0.3
4,944,762 - Pf1N1B4_4483 0.73 +0.2
4,944,766 + Pf1N1B4_4483 0.74 +1.6
4,944,766 + Pf1N1B4_4483 0.74 -0.1
4,944,767 - Pf1N1B4_4483 0.74 +0.8
4,944,820 + +0.3
4,944,821 - +0.9
4,944,977 + -0.4
4,945,021 - -1.6
4,945,083 - -0.2
4,945,085 + -0.3
4,945,086 - -0.1
4,945,180 + Pf1N1B4_4484 0.12 +0.7
4,945,180 + Pf1N1B4_4484 0.12 -2.2
4,945,180 + Pf1N1B4_4484 0.12 +1.2
4,945,184 - Pf1N1B4_4484 0.12 -0.4
4,945,220 - Pf1N1B4_4484 0.15 -1.4
4,945,220 - Pf1N1B4_4484 0.15 +1.1

Or see this region's nucleotide sequence