Experiment: Cholin acetate 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4385 and Pf1N1B4_4386 are separated by 156 nucleotides Pf1N1B4_4386 and Pf1N1B4_4387 are separated by 162 nucleotides Pf1N1B4_4387 and Pf1N1B4_4388 overlap by 4 nucleotides
Pf1N1B4_4385 - Gfa-like protein, at 4,844,117 to 4,844,359
_4385
Pf1N1B4_4386 - Inositol transport system sugar-binding protein, at 4,844,516 to 4,845,442
_4386
Pf1N1B4_4387 - Hydroxypyruvate isomerase (EC 5.3.1.22), at 4,845,605 to 4,846,393
_4387
Pf1N1B4_4388 - Myo-inositol 2-dehydrogenase (EC 1.1.1.18), at 4,846,390 to 4,847,412
_4388
Position (kb)
4844
4845
4846 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4843.536 kb on - strand at 4843.562 kb on - strand at 4843.615 kb on - strand at 4843.852 kb on - strand at 4843.852 kb on - strand at 4844.254 kb on + strand at 4844.254 kb on + strand, within Pf1N1B4_4385 at 4844.254 kb on + strand, within Pf1N1B4_4385 at 4844.255 kb on - strand, within Pf1N1B4_4385 at 4844.255 kb on - strand, within Pf1N1B4_4385 at 4844.255 kb on - strand at 4844.255 kb on - strand, within Pf1N1B4_4385 at 4844.255 kb on - strand, within Pf1N1B4_4385 at 4844.258 kb on + strand, within Pf1N1B4_4385 at 4844.258 kb on + strand at 4844.357 kb on + strand at 4844.358 kb on - strand at 4844.368 kb on + strand at 4844.368 kb on + strand at 4844.369 kb on - strand at 4844.369 kb on - strand at 4844.369 kb on - strand at 4844.402 kb on + strand at 4844.402 kb on + strand at 4844.402 kb on + strand at 4844.402 kb on + strand at 4844.517 kb on + strand at 4844.518 kb on - strand at 4844.518 kb on - strand at 4844.522 kb on - strand at 4844.522 kb on - strand at 4844.524 kb on - strand at 4844.524 kb on - strand at 4844.524 kb on - strand at 4845.327 kb on + strand at 4845.327 kb on + strand, within Pf1N1B4_4386 at 4845.327 kb on + strand at 4845.327 kb on + strand, within Pf1N1B4_4386 at 4845.327 kb on + strand at 4845.327 kb on + strand, within Pf1N1B4_4386 at 4845.327 kb on + strand, within Pf1N1B4_4386 at 4845.328 kb on - strand, within Pf1N1B4_4386 at 4845.328 kb on - strand, within Pf1N1B4_4386 at 4845.328 kb on - strand, within Pf1N1B4_4386 at 4845.408 kb on - strand at 4845.463 kb on + strand at 4845.510 kb on + strand at 4845.511 kb on - strand at 4845.541 kb on - strand at 4845.570 kb on + strand at 4845.571 kb on - strand at 4845.571 kb on - strand at 4845.956 kb on - strand, within Pf1N1B4_4387
Per-strain Table
Position Strand Gene LocusTag Fraction Cholin acetate 10 mM remove 4,843,536 - +0.6 4,843,562 - +0.5 4,843,615 - +1.6 4,843,852 - +0.0 4,843,852 - +0.9 4,844,254 + +0.6 4,844,254 + Pf1N1B4_4385 0.56 +0.0 4,844,254 + Pf1N1B4_4385 0.56 +1.5 4,844,255 - Pf1N1B4_4385 0.57 +0.0 4,844,255 - Pf1N1B4_4385 0.57 -2.8 4,844,255 - -0.4 4,844,255 - Pf1N1B4_4385 0.57 -0.1 4,844,255 - Pf1N1B4_4385 0.57 +0.6 4,844,258 + Pf1N1B4_4385 0.58 +0.0 4,844,258 + -0.4 4,844,357 + -1.8 4,844,358 - -1.4 4,844,368 + +0.4 4,844,368 + -1.4 4,844,369 - -1.1 4,844,369 - +0.2 4,844,369 - +0.6 4,844,402 + +0.1 4,844,402 + -0.4 4,844,402 + -2.0 4,844,402 + +1.4 4,844,517 + +0.5 4,844,518 - -0.4 4,844,518 - +0.4 4,844,522 - -0.0 4,844,522 - +0.0 4,844,524 - +0.0 4,844,524 - +1.4 4,844,524 - +1.0 4,845,327 + +1.9 4,845,327 + Pf1N1B4_4386 0.87 +0.9 4,845,327 + +0.6 4,845,327 + Pf1N1B4_4386 0.87 -0.7 4,845,327 + +2.2 4,845,327 + Pf1N1B4_4386 0.87 -0.6 4,845,327 + Pf1N1B4_4386 0.87 +0.6 4,845,328 - Pf1N1B4_4386 0.88 -1.4 4,845,328 - Pf1N1B4_4386 0.88 +1.6 4,845,328 - Pf1N1B4_4386 0.88 -3.3 4,845,408 - +1.2 4,845,463 + +2.2 4,845,510 + +0.6 4,845,511 - -0.4 4,845,541 - +1.2 4,845,570 + -2.2 4,845,571 - -1.0 4,845,571 - -0.6 4,845,956 - Pf1N1B4_4387 0.44 -1.8
Or see this region's nucleotide sequence