Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_4386

Experiment: Cholin acetate 10 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_4385 and Pf1N1B4_4386 are separated by 156 nucleotidesPf1N1B4_4386 and Pf1N1B4_4387 are separated by 162 nucleotidesPf1N1B4_4387 and Pf1N1B4_4388 overlap by 4 nucleotides Pf1N1B4_4385 - Gfa-like protein, at 4,844,117 to 4,844,359 _4385 Pf1N1B4_4386 - Inositol transport system sugar-binding protein, at 4,844,516 to 4,845,442 _4386 Pf1N1B4_4387 - Hydroxypyruvate isomerase (EC 5.3.1.22), at 4,845,605 to 4,846,393 _4387 Pf1N1B4_4388 - Myo-inositol 2-dehydrogenase (EC 1.1.1.18), at 4,846,390 to 4,847,412 _4388 Position (kb) 4844 4845 4846Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 4843.536 kb on - strandat 4843.562 kb on - strandat 4843.615 kb on - strandat 4843.852 kb on - strandat 4843.852 kb on - strandat 4844.254 kb on + strandat 4844.254 kb on + strand, within Pf1N1B4_4385at 4844.254 kb on + strand, within Pf1N1B4_4385at 4844.255 kb on - strand, within Pf1N1B4_4385at 4844.255 kb on - strand, within Pf1N1B4_4385at 4844.255 kb on - strandat 4844.255 kb on - strand, within Pf1N1B4_4385at 4844.255 kb on - strand, within Pf1N1B4_4385at 4844.258 kb on + strand, within Pf1N1B4_4385at 4844.258 kb on + strandat 4844.357 kb on + strandat 4844.358 kb on - strandat 4844.368 kb on + strandat 4844.368 kb on + strandat 4844.369 kb on - strandat 4844.369 kb on - strandat 4844.369 kb on - strandat 4844.402 kb on + strandat 4844.402 kb on + strandat 4844.402 kb on + strandat 4844.402 kb on + strandat 4844.517 kb on + strandat 4844.518 kb on - strandat 4844.518 kb on - strandat 4844.522 kb on - strandat 4844.522 kb on - strandat 4844.524 kb on - strandat 4844.524 kb on - strandat 4844.524 kb on - strandat 4845.327 kb on + strandat 4845.327 kb on + strand, within Pf1N1B4_4386at 4845.327 kb on + strandat 4845.327 kb on + strand, within Pf1N1B4_4386at 4845.327 kb on + strandat 4845.327 kb on + strand, within Pf1N1B4_4386at 4845.327 kb on + strand, within Pf1N1B4_4386at 4845.328 kb on - strand, within Pf1N1B4_4386at 4845.328 kb on - strand, within Pf1N1B4_4386at 4845.328 kb on - strand, within Pf1N1B4_4386at 4845.408 kb on - strandat 4845.463 kb on + strandat 4845.510 kb on + strandat 4845.511 kb on - strandat 4845.541 kb on - strandat 4845.570 kb on + strandat 4845.571 kb on - strandat 4845.571 kb on - strandat 4845.956 kb on - strand, within Pf1N1B4_4387

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Per-strain Table

Position Strand Gene LocusTag Fraction Cholin acetate 10 mM
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4,843,536 - +0.6
4,843,562 - +0.5
4,843,615 - +1.6
4,843,852 - +0.0
4,843,852 - +0.9
4,844,254 + +0.6
4,844,254 + Pf1N1B4_4385 0.56 +0.0
4,844,254 + Pf1N1B4_4385 0.56 +1.5
4,844,255 - Pf1N1B4_4385 0.57 +0.0
4,844,255 - Pf1N1B4_4385 0.57 -2.8
4,844,255 - -0.4
4,844,255 - Pf1N1B4_4385 0.57 -0.1
4,844,255 - Pf1N1B4_4385 0.57 +0.6
4,844,258 + Pf1N1B4_4385 0.58 +0.0
4,844,258 + -0.4
4,844,357 + -1.8
4,844,358 - -1.4
4,844,368 + +0.4
4,844,368 + -1.4
4,844,369 - -1.1
4,844,369 - +0.2
4,844,369 - +0.6
4,844,402 + +0.1
4,844,402 + -0.4
4,844,402 + -2.0
4,844,402 + +1.4
4,844,517 + +0.5
4,844,518 - -0.4
4,844,518 - +0.4
4,844,522 - -0.0
4,844,522 - +0.0
4,844,524 - +0.0
4,844,524 - +1.4
4,844,524 - +1.0
4,845,327 + +1.9
4,845,327 + Pf1N1B4_4386 0.87 +0.9
4,845,327 + +0.6
4,845,327 + Pf1N1B4_4386 0.87 -0.7
4,845,327 + +2.2
4,845,327 + Pf1N1B4_4386 0.87 -0.6
4,845,327 + Pf1N1B4_4386 0.87 +0.6
4,845,328 - Pf1N1B4_4386 0.88 -1.4
4,845,328 - Pf1N1B4_4386 0.88 +1.6
4,845,328 - Pf1N1B4_4386 0.88 -3.3
4,845,408 - +1.2
4,845,463 + +2.2
4,845,510 + +0.6
4,845,511 - -0.4
4,845,541 - +1.2
4,845,570 + -2.2
4,845,571 - -1.0
4,845,571 - -0.6
4,845,956 - Pf1N1B4_4387 0.44 -1.8

Or see this region's nucleotide sequence