Experiment: Cholin acetate 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4218 and Pf1N1B4_4219 overlap by 4 nucleotides Pf1N1B4_4219 and Pf1N1B4_4220 are separated by 189 nucleotides
Pf1N1B4_4218 - Multidrug resistance protein A, at 4,673,002 to 4,674,099
_4218
Pf1N1B4_4219 - FIG01074225: hypothetical protein, at 4,674,096 to 4,675,691
_4219
Pf1N1B4_4220 - Maebl, at 4,675,881 to 4,676,630
_4220
Position (kb)
4674
4675
4676 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4673.185 kb on + strand, within Pf1N1B4_4218 at 4673.185 kb on + strand, within Pf1N1B4_4218 at 4673.185 kb on + strand at 4673.185 kb on + strand, within Pf1N1B4_4218 at 4673.185 kb on + strand, within Pf1N1B4_4218 at 4673.186 kb on - strand, within Pf1N1B4_4218 at 4673.230 kb on + strand, within Pf1N1B4_4218 at 4673.231 kb on - strand, within Pf1N1B4_4218 at 4673.702 kb on + strand, within Pf1N1B4_4218 at 4673.703 kb on - strand, within Pf1N1B4_4218 at 4673.703 kb on - strand at 4673.845 kb on - strand at 4674.243 kb on + strand at 4674.244 kb on - strand at 4674.244 kb on - strand at 4674.282 kb on + strand, within Pf1N1B4_4219 at 4674.282 kb on + strand, within Pf1N1B4_4219 at 4674.490 kb on + strand, within Pf1N1B4_4219 at 4674.491 kb on - strand at 4674.491 kb on - strand, within Pf1N1B4_4219 at 4674.766 kb on - strand at 4675.084 kb on + strand, within Pf1N1B4_4219 at 4675.316 kb on - strand, within Pf1N1B4_4219 at 4675.560 kb on - strand at 4675.909 kb on + strand at 4675.910 kb on - strand at 4675.997 kb on + strand, within Pf1N1B4_4220 at 4676.153 kb on + strand at 4676.154 kb on - strand, within Pf1N1B4_4220 at 4676.257 kb on + strand, within Pf1N1B4_4220 at 4676.257 kb on + strand, within Pf1N1B4_4220 at 4676.257 kb on + strand, within Pf1N1B4_4220 at 4676.460 kb on - strand, within Pf1N1B4_4220 at 4676.508 kb on + strand, within Pf1N1B4_4220 at 4676.554 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cholin acetate 10 mM remove 4,673,185 + Pf1N1B4_4218 0.17 -0.4 4,673,185 + Pf1N1B4_4218 0.17 -0.0 4,673,185 + -0.4 4,673,185 + Pf1N1B4_4218 0.17 +0.6 4,673,185 + Pf1N1B4_4218 0.17 +0.6 4,673,186 - Pf1N1B4_4218 0.17 +0.1 4,673,230 + Pf1N1B4_4218 0.21 -0.5 4,673,231 - Pf1N1B4_4218 0.21 -0.9 4,673,702 + Pf1N1B4_4218 0.64 +0.1 4,673,703 - Pf1N1B4_4218 0.64 -1.7 4,673,703 - +2.5 4,673,845 - +0.6 4,674,243 + +0.1 4,674,244 - +0.1 4,674,244 - +0.5 4,674,282 + Pf1N1B4_4219 0.12 -0.4 4,674,282 + Pf1N1B4_4219 0.12 -0.6 4,674,490 + Pf1N1B4_4219 0.25 +0.1 4,674,491 - +0.9 4,674,491 - Pf1N1B4_4219 0.25 -1.7 4,674,766 - +0.2 4,675,084 + Pf1N1B4_4219 0.62 +1.6 4,675,316 - Pf1N1B4_4219 0.76 -0.8 4,675,560 - +0.2 4,675,909 + -2.4 4,675,910 - +1.1 4,675,997 + Pf1N1B4_4220 0.15 +0.2 4,676,153 + +1.2 4,676,154 - Pf1N1B4_4220 0.36 +2.2 4,676,257 + Pf1N1B4_4220 0.50 +2.6 4,676,257 + Pf1N1B4_4220 0.50 -0.9 4,676,257 + Pf1N1B4_4220 0.50 -0.4 4,676,460 - Pf1N1B4_4220 0.77 +1.1 4,676,508 + Pf1N1B4_4220 0.84 +0.1 4,676,554 + -0.9
Or see this region's nucleotide sequence