Experiment: Cholin acetate 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4107 and Pf1N1B4_4108 are separated by 15 nucleotides Pf1N1B4_4108 and Pf1N1B4_4109 are separated by 133 nucleotides Pf1N1B4_4109 and Pf1N1B4_4110 are separated by 130 nucleotides Pf1N1B4_4110 and Pf1N1B4_4111 are separated by 173 nucleotides
Pf1N1B4_4107 - Probable glutathione S-transferase (EC 2.5.1.18), YfcF homolog, at 4,554,273 to 4,554,905
_4107
Pf1N1B4_4108 - Hydrolase, alpha/beta fold family, at 4,554,921 to 4,555,718
_4108
Pf1N1B4_4109 - Cytochrome c2, at 4,555,852 to 4,556,241
_4109
Pf1N1B4_4110 - hypothetical protein, at 4,556,372 to 4,556,707
_4110
Pf1N1B4_4111 - Panthothenate synthetase, at 4,556,881 to 4,557,171
_4111
Position (kb)
4555
4556
4557 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4554.903 kb on + strand at 4554.935 kb on + strand at 4554.935 kb on + strand at 4554.974 kb on + strand at 4554.986 kb on + strand at 4554.986 kb on + strand at 4554.986 kb on + strand at 4555.385 kb on - strand, within Pf1N1B4_4108 at 4555.385 kb on - strand, within Pf1N1B4_4108 at 4555.450 kb on + strand, within Pf1N1B4_4108 at 4555.451 kb on - strand, within Pf1N1B4_4108 at 4555.451 kb on - strand, within Pf1N1B4_4108 at 4555.451 kb on - strand at 4555.451 kb on - strand, within Pf1N1B4_4108 at 4555.697 kb on - strand at 4555.761 kb on + strand at 4555.761 kb on + strand at 4555.762 kb on - strand at 4555.762 kb on - strand at 4555.762 kb on - strand at 4555.762 kb on - strand at 4555.762 kb on - strand at 4555.762 kb on - strand at 4555.762 kb on - strand at 4555.762 kb on - strand at 4555.762 kb on - strand at 4555.767 kb on - strand at 4555.767 kb on - strand at 4555.786 kb on - strand at 4555.952 kb on + strand, within Pf1N1B4_4109 at 4555.952 kb on + strand, within Pf1N1B4_4109 at 4555.953 kb on - strand, within Pf1N1B4_4109 at 4555.953 kb on - strand at 4555.953 kb on - strand at 4555.962 kb on + strand, within Pf1N1B4_4109 at 4555.963 kb on - strand, within Pf1N1B4_4109 at 4555.985 kb on + strand, within Pf1N1B4_4109 at 4555.986 kb on - strand, within Pf1N1B4_4109 at 4556.062 kb on + strand at 4556.063 kb on - strand, within Pf1N1B4_4109 at 4556.068 kb on + strand, within Pf1N1B4_4109 at 4556.119 kb on - strand, within Pf1N1B4_4109 at 4556.125 kb on + strand, within Pf1N1B4_4109 at 4556.147 kb on + strand, within Pf1N1B4_4109 at 4556.216 kb on - strand at 4556.251 kb on + strand at 4556.378 kb on + strand at 4556.379 kb on - strand at 4556.379 kb on - strand at 4556.379 kb on - strand at 4556.379 kb on - strand at 4556.379 kb on - strand at 4556.474 kb on + strand, within Pf1N1B4_4110 at 4556.474 kb on + strand, within Pf1N1B4_4110 at 4556.475 kb on - strand, within Pf1N1B4_4110 at 4556.475 kb on - strand, within Pf1N1B4_4110 at 4557.020 kb on - strand, within Pf1N1B4_4111 at 4557.020 kb on - strand, within Pf1N1B4_4111 at 4557.020 kb on - strand, within Pf1N1B4_4111 at 4557.020 kb on - strand, within Pf1N1B4_4111 at 4557.020 kb on - strand, within Pf1N1B4_4111 at 4557.188 kb on - strand at 4557.194 kb on + strand at 4557.231 kb on + strand at 4557.231 kb on + strand at 4557.231 kb on + strand at 4557.232 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cholin acetate 10 mM remove 4,554,903 + -1.7 4,554,935 + +0.1 4,554,935 + +0.3 4,554,974 + -0.9 4,554,986 + +1.2 4,554,986 + -0.3 4,554,986 + +2.0 4,555,385 - Pf1N1B4_4108 0.58 -1.1 4,555,385 - Pf1N1B4_4108 0.58 +1.3 4,555,450 + Pf1N1B4_4108 0.66 -0.3 4,555,451 - Pf1N1B4_4108 0.66 +1.4 4,555,451 - Pf1N1B4_4108 0.66 -1.3 4,555,451 - -0.3 4,555,451 - Pf1N1B4_4108 0.66 -1.7 4,555,697 - +2.2 4,555,761 + +0.7 4,555,761 + +0.1 4,555,762 - -0.1 4,555,762 - -0.3 4,555,762 - -0.7 4,555,762 - +0.5 4,555,762 - -1.9 4,555,762 - -2.1 4,555,762 - -0.1 4,555,762 - +0.1 4,555,762 - +1.0 4,555,767 - +1.7 4,555,767 - +0.7 4,555,786 - +0.4 4,555,952 + Pf1N1B4_4109 0.26 +0.1 4,555,952 + Pf1N1B4_4109 0.26 +0.7 4,555,953 - Pf1N1B4_4109 0.26 -2.0 4,555,953 - -0.7 4,555,953 - -0.9 4,555,962 + Pf1N1B4_4109 0.28 -1.3 4,555,963 - Pf1N1B4_4109 0.28 -1.1 4,555,985 + Pf1N1B4_4109 0.34 -0.5 4,555,986 - Pf1N1B4_4109 0.34 -0.3 4,556,062 + -0.3 4,556,063 - Pf1N1B4_4109 0.54 -1.5 4,556,068 + Pf1N1B4_4109 0.55 -0.9 4,556,119 - Pf1N1B4_4109 0.68 +1.9 4,556,125 + Pf1N1B4_4109 0.70 -2.8 4,556,147 + Pf1N1B4_4109 0.76 -0.3 4,556,216 - +0.7 4,556,251 + +1.1 4,556,378 + -0.3 4,556,379 - +0.7 4,556,379 - +0.2 4,556,379 - -0.3 4,556,379 - +0.7 4,556,379 - -0.5 4,556,474 + Pf1N1B4_4110 0.30 +0.9 4,556,474 + Pf1N1B4_4110 0.30 +0.7 4,556,475 - Pf1N1B4_4110 0.31 +0.2 4,556,475 - Pf1N1B4_4110 0.31 +1.0 4,557,020 - Pf1N1B4_4111 0.48 +0.0 4,557,020 - Pf1N1B4_4111 0.48 +0.2 4,557,020 - Pf1N1B4_4111 0.48 -2.5 4,557,020 - Pf1N1B4_4111 0.48 +0.4 4,557,020 - Pf1N1B4_4111 0.48 -1.3 4,557,188 - -0.5 4,557,194 + -0.7 4,557,231 + -0.3 4,557,231 + -1.1 4,557,231 + +0.1 4,557,232 - +1.1
Or see this region's nucleotide sequence