Experiment: Cholin acetate 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3995 and Pf1N1B4_3996 are separated by 244 nucleotides Pf1N1B4_3996 and Pf1N1B4_3997 are separated by 65 nucleotides Pf1N1B4_3997 and Pf1N1B4_3998 overlap by 4 nucleotides
Pf1N1B4_3995 - Exodeoxyribonuclease III (EC 3.1.11.2), at 4,440,105 to 4,440,917
_3995
Pf1N1B4_3996 - Histone acetyltransferase HPA2 and related acetyltransferases, at 4,441,162 to 4,441,812
_3996
Pf1N1B4_3997 - Uncharacterized protein YtfM precursor, at 4,441,878 to 4,443,605
_3997
Pf1N1B4_3998 - putative exported protein, at 4,443,602 to 4,447,276
_3998
Position (kb)
4441
4442
4443
4444 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 4440.948 kb on - strand at 4440.948 kb on - strand at 4440.958 kb on + strand at 4440.958 kb on + strand at 4440.958 kb on + strand at 4440.959 kb on - strand at 4440.959 kb on - strand at 4440.959 kb on - strand at 4441.071 kb on + strand at 4441.071 kb on + strand at 4441.071 kb on + strand at 4441.071 kb on + strand at 4441.072 kb on - strand at 4441.074 kb on - strand at 4441.074 kb on - strand at 4441.117 kb on + strand at 4441.240 kb on + strand, within Pf1N1B4_3996 at 4441.241 kb on - strand at 4441.633 kb on - strand, within Pf1N1B4_3996 at 4441.705 kb on + strand, within Pf1N1B4_3996 at 4441.705 kb on + strand, within Pf1N1B4_3996 at 4441.705 kb on + strand, within Pf1N1B4_3996 at 4441.706 kb on - strand, within Pf1N1B4_3996 at 4441.706 kb on - strand, within Pf1N1B4_3996 at 4441.706 kb on - strand, within Pf1N1B4_3996 at 4441.706 kb on - strand, within Pf1N1B4_3996 at 4441.706 kb on - strand, within Pf1N1B4_3996 at 4441.706 kb on - strand, within Pf1N1B4_3996 at 4441.706 kb on - strand, within Pf1N1B4_3996 at 4441.706 kb on - strand, within Pf1N1B4_3996 at 4441.706 kb on - strand at 4441.706 kb on - strand, within Pf1N1B4_3996 at 4441.706 kb on - strand, within Pf1N1B4_3996 at 4441.767 kb on - strand at 4441.794 kb on - strand at 4441.816 kb on + strand at 4441.842 kb on + strand at 4441.843 kb on - strand at 4441.843 kb on - strand at 4441.865 kb on - strand at 4441.865 kb on - strand at 4441.999 kb on + strand at 4441.999 kb on + strand at 4441.999 kb on + strand at 4441.999 kb on + strand at 4441.999 kb on + strand at 4441.999 kb on + strand at 4442.000 kb on - strand at 4442.000 kb on - strand at 4442.000 kb on - strand at 4442.000 kb on - strand at 4442.007 kb on + strand at 4442.007 kb on + strand at 4442.007 kb on + strand at 4442.007 kb on + strand at 4442.008 kb on - strand at 4442.285 kb on + strand, within Pf1N1B4_3997 at 4442.580 kb on + strand, within Pf1N1B4_3997 at 4442.817 kb on + strand, within Pf1N1B4_3997 at 4442.818 kb on - strand, within Pf1N1B4_3997 at 4443.052 kb on - strand, within Pf1N1B4_3997 at 4443.159 kb on + strand, within Pf1N1B4_3997 at 4443.160 kb on - strand, within Pf1N1B4_3997 at 4443.400 kb on - strand, within Pf1N1B4_3997
Per-strain Table
Position Strand Gene LocusTag Fraction Cholin acetate 10 mM remove 4,440,948 - +0.9 4,440,948 - -0.3 4,440,958 + +0.6 4,440,958 + +0.7 4,440,958 + +0.5 4,440,959 - +0.1 4,440,959 - +0.7 4,440,959 - -1.1 4,441,071 + +0.7 4,441,071 + -0.9 4,441,071 + -0.3 4,441,071 + -0.3 4,441,072 - +0.2 4,441,074 - -0.3 4,441,074 - -0.7 4,441,117 + +2.3 4,441,240 + Pf1N1B4_3996 0.12 +1.3 4,441,241 - +0.7 4,441,633 - Pf1N1B4_3996 0.72 +0.2 4,441,705 + Pf1N1B4_3996 0.83 -0.3 4,441,705 + Pf1N1B4_3996 0.83 +1.7 4,441,705 + Pf1N1B4_3996 0.83 -0.1 4,441,706 - Pf1N1B4_3996 0.84 -0.1 4,441,706 - Pf1N1B4_3996 0.84 +2.3 4,441,706 - Pf1N1B4_3996 0.84 +0.1 4,441,706 - Pf1N1B4_3996 0.84 +0.7 4,441,706 - Pf1N1B4_3996 0.84 -0.3 4,441,706 - Pf1N1B4_3996 0.84 -1.0 4,441,706 - Pf1N1B4_3996 0.84 -1.1 4,441,706 - Pf1N1B4_3996 0.84 +1.7 4,441,706 - +3.3 4,441,706 - Pf1N1B4_3996 0.84 -0.3 4,441,706 - Pf1N1B4_3996 0.84 -0.3 4,441,767 - -0.0 4,441,794 - +0.4 4,441,816 + +1.5 4,441,842 + -0.1 4,441,843 - +0.1 4,441,843 - +1.5 4,441,865 - +0.1 4,441,865 - +0.7 4,441,999 + -0.3 4,441,999 + -2.1 4,441,999 + +0.7 4,441,999 + -0.3 4,441,999 + -0.3 4,441,999 + -0.7 4,442,000 - -1.0 4,442,000 - -0.9 4,442,000 - -0.2 4,442,000 - -0.4 4,442,007 + -0.9 4,442,007 + -0.6 4,442,007 + -3.1 4,442,007 + -1.9 4,442,008 - +0.0 4,442,285 + Pf1N1B4_3997 0.24 -0.6 4,442,580 + Pf1N1B4_3997 0.41 -1.6 4,442,817 + Pf1N1B4_3997 0.54 -1.6 4,442,818 - Pf1N1B4_3997 0.54 +0.3 4,443,052 - Pf1N1B4_3997 0.68 -0.1 4,443,159 + Pf1N1B4_3997 0.74 -2.3 4,443,160 - Pf1N1B4_3997 0.74 -0.9 4,443,400 - Pf1N1B4_3997 0.88 -2.0
Or see this region's nucleotide sequence