Experiment: Cholin acetate 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3221 and Pf1N1B4_3222 are separated by 149 nucleotides Pf1N1B4_3222 and Pf1N1B4_3223 are separated by 465 nucleotides
Pf1N1B4_3221 - Sensory box/GGDEF family protein, at 3,604,868 to 3,607,150
_3221
Pf1N1B4_3222 - Ferredoxin, at 3,607,300 to 3,607,569
_3222
Pf1N1B4_3223 - Major facilitator family transporter, at 3,608,035 to 3,609,384
_3223
Position (kb)
3607
3608 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3606.567 kb on + strand, within Pf1N1B4_3221 at 3606.567 kb on + strand, within Pf1N1B4_3221 at 3606.567 kb on + strand, within Pf1N1B4_3221 at 3606.567 kb on + strand, within Pf1N1B4_3221 at 3606.567 kb on + strand, within Pf1N1B4_3221 at 3606.567 kb on + strand, within Pf1N1B4_3221 at 3606.567 kb on + strand, within Pf1N1B4_3221 at 3606.568 kb on - strand, within Pf1N1B4_3221 at 3606.568 kb on - strand, within Pf1N1B4_3221 at 3606.568 kb on - strand, within Pf1N1B4_3221 at 3606.684 kb on + strand, within Pf1N1B4_3221 at 3606.685 kb on - strand, within Pf1N1B4_3221 at 3606.685 kb on - strand, within Pf1N1B4_3221 at 3606.726 kb on + strand, within Pf1N1B4_3221 at 3606.726 kb on + strand, within Pf1N1B4_3221 at 3606.726 kb on + strand, within Pf1N1B4_3221 at 3606.726 kb on + strand at 3606.727 kb on - strand, within Pf1N1B4_3221 at 3606.727 kb on - strand, within Pf1N1B4_3221 at 3606.727 kb on - strand, within Pf1N1B4_3221 at 3606.727 kb on - strand, within Pf1N1B4_3221 at 3606.727 kb on - strand, within Pf1N1B4_3221 at 3606.727 kb on - strand, within Pf1N1B4_3221 at 3606.727 kb on - strand, within Pf1N1B4_3221 at 3606.727 kb on - strand, within Pf1N1B4_3221 at 3606.774 kb on + strand, within Pf1N1B4_3221 at 3606.774 kb on + strand, within Pf1N1B4_3221 at 3607.053 kb on + strand at 3607.079 kb on - strand at 3607.094 kb on + strand at 3607.095 kb on - strand at 3607.095 kb on - strand at 3607.095 kb on - strand at 3607.095 kb on - strand at 3607.152 kb on - strand at 3607.213 kb on - strand at 3607.213 kb on - strand at 3607.213 kb on - strand at 3607.213 kb on - strand at 3607.214 kb on + strand at 3607.214 kb on + strand at 3607.214 kb on + strand at 3607.214 kb on + strand at 3607.214 kb on + strand at 3607.215 kb on - strand at 3607.294 kb on + strand at 3607.295 kb on - strand at 3607.316 kb on + strand at 3607.317 kb on - strand at 3607.480 kb on + strand, within Pf1N1B4_3222 at 3607.480 kb on + strand, within Pf1N1B4_3222 at 3607.481 kb on - strand, within Pf1N1B4_3222 at 3607.720 kb on + strand at 3607.720 kb on + strand at 3607.721 kb on - strand at 3607.721 kb on - strand at 3607.721 kb on - strand at 3607.758 kb on + strand at 3607.758 kb on + strand at 3607.759 kb on - strand at 3607.897 kb on + strand at 3607.897 kb on + strand at 3607.897 kb on + strand at 3607.898 kb on - strand at 3607.898 kb on - strand at 3607.980 kb on + strand at 3607.980 kb on + strand at 3607.980 kb on + strand at 3607.981 kb on - strand at 3607.981 kb on - strand at 3607.981 kb on - strand at 3607.981 kb on - strand at 3607.981 kb on - strand at 3607.981 kb on - strand at 3608.014 kb on + strand at 3608.015 kb on - strand at 3608.015 kb on - strand at 3608.270 kb on + strand, within Pf1N1B4_3223 at 3608.270 kb on + strand, within Pf1N1B4_3223 at 3608.271 kb on - strand, within Pf1N1B4_3223 at 3608.383 kb on + strand, within Pf1N1B4_3223 at 3608.384 kb on - strand, within Pf1N1B4_3223 at 3608.451 kb on + strand, within Pf1N1B4_3223 at 3608.451 kb on + strand, within Pf1N1B4_3223 at 3608.451 kb on + strand, within Pf1N1B4_3223 at 3608.451 kb on + strand, within Pf1N1B4_3223 at 3608.451 kb on + strand, within Pf1N1B4_3223 at 3608.452 kb on - strand, within Pf1N1B4_3223 at 3608.481 kb on - strand, within Pf1N1B4_3223
Per-strain Table
Position Strand Gene LocusTag Fraction Cholin acetate 10 mM remove 3,606,567 + Pf1N1B4_3221 0.74 +1.0 3,606,567 + Pf1N1B4_3221 0.74 +1.2 3,606,567 + Pf1N1B4_3221 0.74 -0.0 3,606,567 + Pf1N1B4_3221 0.74 -0.8 3,606,567 + Pf1N1B4_3221 0.74 +2.3 3,606,567 + Pf1N1B4_3221 0.74 +1.5 3,606,567 + Pf1N1B4_3221 0.74 +0.2 3,606,568 - Pf1N1B4_3221 0.74 +1.0 3,606,568 - Pf1N1B4_3221 0.74 -1.2 3,606,568 - Pf1N1B4_3221 0.74 +1.0 3,606,684 + Pf1N1B4_3221 0.80 +1.0 3,606,685 - Pf1N1B4_3221 0.80 -1.4 3,606,685 - Pf1N1B4_3221 0.80 +0.0 3,606,726 + Pf1N1B4_3221 0.81 +0.1 3,606,726 + Pf1N1B4_3221 0.81 +0.2 3,606,726 + Pf1N1B4_3221 0.81 -0.0 3,606,726 + +1.5 3,606,727 - Pf1N1B4_3221 0.81 -0.2 3,606,727 - Pf1N1B4_3221 0.81 -2.6 3,606,727 - Pf1N1B4_3221 0.81 +1.5 3,606,727 - Pf1N1B4_3221 0.81 +0.3 3,606,727 - Pf1N1B4_3221 0.81 +0.6 3,606,727 - Pf1N1B4_3221 0.81 +1.8 3,606,727 - Pf1N1B4_3221 0.81 +0.5 3,606,727 - Pf1N1B4_3221 0.81 -1.2 3,606,774 + Pf1N1B4_3221 0.83 +0.2 3,606,774 + Pf1N1B4_3221 0.83 -1.4 3,607,053 + -0.6 3,607,079 - -0.0 3,607,094 + -0.3 3,607,095 - +0.6 3,607,095 - +0.2 3,607,095 - -1.0 3,607,095 - +0.6 3,607,152 - -0.6 3,607,213 - +0.3 3,607,213 - +0.5 3,607,213 - +0.2 3,607,213 - +1.4 3,607,214 + -0.2 3,607,214 + +0.7 3,607,214 + +0.2 3,607,214 + +0.2 3,607,214 + -0.4 3,607,215 - -1.6 3,607,294 + +0.3 3,607,295 - -0.6 3,607,316 + -0.7 3,607,317 - -2.2 3,607,480 + Pf1N1B4_3222 0.67 -1.0 3,607,480 + Pf1N1B4_3222 0.67 -0.6 3,607,481 - Pf1N1B4_3222 0.67 +0.5 3,607,720 + +0.1 3,607,720 + -0.6 3,607,721 - -0.6 3,607,721 - -0.7 3,607,721 - -0.5 3,607,758 + +0.2 3,607,758 + +1.2 3,607,759 - +0.8 3,607,897 + -0.2 3,607,897 + +1.1 3,607,897 + +0.6 3,607,898 - -0.6 3,607,898 - -1.6 3,607,980 + +0.1 3,607,980 + +2.2 3,607,980 + +0.7 3,607,981 - -0.6 3,607,981 - +2.0 3,607,981 - +2.2 3,607,981 - +0.7 3,607,981 - -0.0 3,607,981 - -0.4 3,608,014 + +0.5 3,608,015 - -0.4 3,608,015 - -0.9 3,608,270 + Pf1N1B4_3223 0.17 +0.4 3,608,270 + Pf1N1B4_3223 0.17 -2.6 3,608,271 - Pf1N1B4_3223 0.17 +0.2 3,608,383 + Pf1N1B4_3223 0.26 -1.0 3,608,384 - Pf1N1B4_3223 0.26 +1.4 3,608,451 + Pf1N1B4_3223 0.31 -0.6 3,608,451 + Pf1N1B4_3223 0.31 +0.5 3,608,451 + Pf1N1B4_3223 0.31 -0.4 3,608,451 + Pf1N1B4_3223 0.31 -0.0 3,608,451 + Pf1N1B4_3223 0.31 -0.6 3,608,452 - Pf1N1B4_3223 0.31 +1.0 3,608,481 - Pf1N1B4_3223 0.33 -2.0
Or see this region's nucleotide sequence