Experiment: Cholin acetate 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3209 and Pf1N1B4_3210 overlap by 4 nucleotides Pf1N1B4_3210 and Pf1N1B4_3211 are separated by 25 nucleotides Pf1N1B4_3211 and Pf1N1B4_3212 are separated by 116 nucleotides Pf1N1B4_3212 and Pf1N1B4_3213 overlap by 7 nucleotides
Pf1N1B4_3209 - Glutamate synthase [NADPH] large chain (EC 1.4.1.13), at 3,592,424 to 3,593,182
_3209
Pf1N1B4_3210 - Uncharacterized protein conserved in bacteria, NMA0228-like, at 3,593,179 to 3,594,033
_3210
Pf1N1B4_3211 - Putative signal peptide protein, at 3,594,059 to 3,594,364
_3211
Pf1N1B4_3212 - hypothetical protein, at 3,594,481 to 3,595,173
_3212
Pf1N1B4_3213 - hypothetical protein, at 3,595,167 to 3,595,376
_3213
Position (kb)
3594
3595 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3593.062 kb on + strand, within Pf1N1B4_3209 at 3593.063 kb on - strand, within Pf1N1B4_3209 at 3593.063 kb on - strand, within Pf1N1B4_3209 at 3593.073 kb on + strand, within Pf1N1B4_3209 at 3593.073 kb on + strand, within Pf1N1B4_3209 at 3593.255 kb on - strand at 3593.315 kb on + strand, within Pf1N1B4_3210 at 3593.315 kb on + strand, within Pf1N1B4_3210 at 3593.388 kb on + strand, within Pf1N1B4_3210 at 3593.389 kb on - strand, within Pf1N1B4_3210 at 3593.389 kb on - strand, within Pf1N1B4_3210 at 3593.451 kb on - strand, within Pf1N1B4_3210 at 3593.451 kb on - strand, within Pf1N1B4_3210 at 3593.489 kb on + strand, within Pf1N1B4_3210 at 3593.490 kb on - strand, within Pf1N1B4_3210 at 3593.490 kb on - strand, within Pf1N1B4_3210 at 3593.490 kb on - strand, within Pf1N1B4_3210 at 3593.490 kb on - strand, within Pf1N1B4_3210 at 3593.588 kb on + strand, within Pf1N1B4_3210 at 3593.588 kb on + strand, within Pf1N1B4_3210 at 3593.589 kb on - strand, within Pf1N1B4_3210 at 3593.589 kb on - strand, within Pf1N1B4_3210 at 3593.589 kb on - strand, within Pf1N1B4_3210 at 3593.646 kb on + strand, within Pf1N1B4_3210 at 3593.647 kb on - strand, within Pf1N1B4_3210 at 3593.682 kb on - strand, within Pf1N1B4_3210 at 3593.826 kb on + strand, within Pf1N1B4_3210 at 3593.826 kb on + strand, within Pf1N1B4_3210 at 3593.826 kb on + strand, within Pf1N1B4_3210 at 3593.826 kb on + strand, within Pf1N1B4_3210 at 3593.827 kb on - strand, within Pf1N1B4_3210 at 3593.858 kb on + strand, within Pf1N1B4_3210 at 3593.858 kb on + strand, within Pf1N1B4_3210 at 3593.858 kb on + strand, within Pf1N1B4_3210 at 3593.912 kb on + strand, within Pf1N1B4_3210 at 3593.912 kb on + strand at 3593.912 kb on + strand at 3593.912 kb on + strand, within Pf1N1B4_3210 at 3593.913 kb on - strand, within Pf1N1B4_3210 at 3593.913 kb on - strand, within Pf1N1B4_3210 at 3593.913 kb on - strand, within Pf1N1B4_3210 at 3594.075 kb on + strand at 3594.075 kb on + strand at 3594.075 kb on + strand at 3594.076 kb on - strand at 3594.076 kb on - strand at 3594.076 kb on - strand at 3594.076 kb on - strand at 3594.076 kb on - strand at 3594.076 kb on - strand at 3594.196 kb on + strand, within Pf1N1B4_3211 at 3594.196 kb on + strand, within Pf1N1B4_3211 at 3594.196 kb on + strand, within Pf1N1B4_3211 at 3594.196 kb on + strand, within Pf1N1B4_3211 at 3594.197 kb on - strand, within Pf1N1B4_3211 at 3594.197 kb on - strand, within Pf1N1B4_3211 at 3594.197 kb on - strand, within Pf1N1B4_3211 at 3594.197 kb on - strand, within Pf1N1B4_3211 at 3594.197 kb on - strand at 3594.197 kb on - strand, within Pf1N1B4_3211 at 3594.199 kb on + strand, within Pf1N1B4_3211 at 3594.413 kb on + strand at 3594.413 kb on + strand at 3594.414 kb on - strand at 3594.498 kb on + strand at 3594.593 kb on + strand at 3594.594 kb on - strand, within Pf1N1B4_3212 at 3594.594 kb on - strand, within Pf1N1B4_3212 at 3594.620 kb on + strand, within Pf1N1B4_3212 at 3594.696 kb on - strand, within Pf1N1B4_3212 at 3594.785 kb on + strand, within Pf1N1B4_3212 at 3594.786 kb on - strand, within Pf1N1B4_3212 at 3594.870 kb on - strand, within Pf1N1B4_3212 at 3594.870 kb on - strand, within Pf1N1B4_3212 at 3595.148 kb on - strand at 3595.227 kb on - strand, within Pf1N1B4_3213
Per-strain Table
Position Strand Gene LocusTag Fraction Cholin acetate 10 mM remove 3,593,062 + Pf1N1B4_3209 0.84 -1.5 3,593,063 - Pf1N1B4_3209 0.84 -0.0 3,593,063 - Pf1N1B4_3209 0.84 +0.5 3,593,073 + Pf1N1B4_3209 0.86 -1.4 3,593,073 + Pf1N1B4_3209 0.86 -0.6 3,593,255 - -2.2 3,593,315 + Pf1N1B4_3210 0.16 -1.0 3,593,315 + Pf1N1B4_3210 0.16 +0.9 3,593,388 + Pf1N1B4_3210 0.24 +0.2 3,593,389 - Pf1N1B4_3210 0.25 +1.0 3,593,389 - Pf1N1B4_3210 0.25 -0.3 3,593,451 - Pf1N1B4_3210 0.32 -0.0 3,593,451 - Pf1N1B4_3210 0.32 -0.9 3,593,489 + Pf1N1B4_3210 0.36 +0.2 3,593,490 - Pf1N1B4_3210 0.36 +1.0 3,593,490 - Pf1N1B4_3210 0.36 -1.4 3,593,490 - Pf1N1B4_3210 0.36 -0.0 3,593,490 - Pf1N1B4_3210 0.36 -1.3 3,593,588 + Pf1N1B4_3210 0.48 +1.0 3,593,588 + Pf1N1B4_3210 0.48 +0.6 3,593,589 - Pf1N1B4_3210 0.48 -0.4 3,593,589 - Pf1N1B4_3210 0.48 -1.6 3,593,589 - Pf1N1B4_3210 0.48 -0.8 3,593,646 + Pf1N1B4_3210 0.55 -0.7 3,593,647 - Pf1N1B4_3210 0.55 +0.5 3,593,682 - Pf1N1B4_3210 0.59 -1.0 3,593,826 + Pf1N1B4_3210 0.76 +0.8 3,593,826 + Pf1N1B4_3210 0.76 -0.0 3,593,826 + Pf1N1B4_3210 0.76 -0.4 3,593,826 + Pf1N1B4_3210 0.76 -1.0 3,593,827 - Pf1N1B4_3210 0.76 -0.0 3,593,858 + Pf1N1B4_3210 0.79 -0.0 3,593,858 + Pf1N1B4_3210 0.79 -0.0 3,593,858 + Pf1N1B4_3210 0.79 -0.0 3,593,912 + Pf1N1B4_3210 0.86 -1.0 3,593,912 + -0.0 3,593,912 + -1.0 3,593,912 + Pf1N1B4_3210 0.86 +0.7 3,593,913 - Pf1N1B4_3210 0.86 -0.6 3,593,913 - Pf1N1B4_3210 0.86 -0.5 3,593,913 - Pf1N1B4_3210 0.86 -1.8 3,594,075 + -0.4 3,594,075 + -1.0 3,594,075 + -1.6 3,594,076 - -0.6 3,594,076 - -0.6 3,594,076 - -0.6 3,594,076 - +1.2 3,594,076 - -0.6 3,594,076 - +0.6 3,594,196 + Pf1N1B4_3211 0.45 -0.7 3,594,196 + Pf1N1B4_3211 0.45 -0.6 3,594,196 + Pf1N1B4_3211 0.45 -0.5 3,594,196 + Pf1N1B4_3211 0.45 -0.6 3,594,197 - Pf1N1B4_3211 0.45 -0.6 3,594,197 - Pf1N1B4_3211 0.45 -0.1 3,594,197 - Pf1N1B4_3211 0.45 -0.5 3,594,197 - Pf1N1B4_3211 0.45 -1.8 3,594,197 - +2.0 3,594,197 - Pf1N1B4_3211 0.45 +0.4 3,594,199 + Pf1N1B4_3211 0.46 -0.3 3,594,413 + -1.0 3,594,413 + -0.6 3,594,414 - +0.4 3,594,498 + -0.8 3,594,593 + -1.0 3,594,594 - Pf1N1B4_3212 0.16 +0.4 3,594,594 - Pf1N1B4_3212 0.16 -0.4 3,594,620 + Pf1N1B4_3212 0.20 -0.8 3,594,696 - Pf1N1B4_3212 0.31 -0.2 3,594,785 + Pf1N1B4_3212 0.44 -1.0 3,594,786 - Pf1N1B4_3212 0.44 +1.0 3,594,870 - Pf1N1B4_3212 0.56 -0.0 3,594,870 - Pf1N1B4_3212 0.56 +1.3 3,595,148 - -1.0 3,595,227 - Pf1N1B4_3213 0.29 -0.2
Or see this region's nucleotide sequence