Experiment: Cholin acetate 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2850 and Pf1N1B4_2851 overlap by 24 nucleotides Pf1N1B4_2851 and Pf1N1B4_2852 are separated by 111 nucleotides Pf1N1B4_2852 and Pf1N1B4_2853 are separated by 195 nucleotides
Pf1N1B4_2850 - Glucose-6-phosphate isomerase (EC 5.3.1.9), at 3,197,753 to 3,199,417
_2850
Pf1N1B4_2851 - hypothetical protein, at 3,199,394 to 3,199,537
_2851
Pf1N1B4_2852 - Aspartate 1-decarboxylase (EC 4.1.1.11), at 3,199,649 to 3,200,029
_2852
Pf1N1B4_2853 - Pantoate--beta-alanine ligase (EC 6.3.2.1), at 3,200,225 to 3,201,085
_2853
Position (kb)
3199
3200 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3198.436 kb on - strand, within Pf1N1B4_2850 at 3198.513 kb on + strand, within Pf1N1B4_2850 at 3198.513 kb on + strand, within Pf1N1B4_2850 at 3198.513 kb on + strand, within Pf1N1B4_2850 at 3198.513 kb on + strand, within Pf1N1B4_2850 at 3198.513 kb on + strand, within Pf1N1B4_2850 at 3198.513 kb on + strand, within Pf1N1B4_2850 at 3198.514 kb on - strand, within Pf1N1B4_2850 at 3198.595 kb on - strand, within Pf1N1B4_2850 at 3198.696 kb on + strand, within Pf1N1B4_2850 at 3198.867 kb on + strand at 3198.868 kb on - strand, within Pf1N1B4_2850 at 3199.049 kb on + strand, within Pf1N1B4_2850 at 3199.049 kb on + strand, within Pf1N1B4_2850 at 3199.049 kb on + strand, within Pf1N1B4_2850 at 3199.049 kb on + strand, within Pf1N1B4_2850 at 3199.049 kb on + strand, within Pf1N1B4_2850 at 3199.049 kb on + strand, within Pf1N1B4_2850 at 3199.049 kb on + strand, within Pf1N1B4_2850 at 3199.049 kb on + strand, within Pf1N1B4_2850 at 3199.049 kb on + strand, within Pf1N1B4_2850 at 3199.050 kb on - strand, within Pf1N1B4_2850 at 3199.050 kb on - strand, within Pf1N1B4_2850 at 3199.050 kb on - strand, within Pf1N1B4_2850 at 3199.050 kb on - strand, within Pf1N1B4_2850 at 3199.050 kb on - strand, within Pf1N1B4_2850 at 3199.050 kb on - strand, within Pf1N1B4_2850 at 3199.252 kb on - strand at 3199.291 kb on + strand at 3199.291 kb on + strand at 3199.410 kb on + strand at 3199.410 kb on + strand at 3199.410 kb on + strand at 3199.411 kb on - strand at 3199.411 kb on - strand at 3199.436 kb on + strand, within Pf1N1B4_2851 at 3199.437 kb on - strand, within Pf1N1B4_2851 at 3199.437 kb on - strand, within Pf1N1B4_2851 at 3199.461 kb on + strand, within Pf1N1B4_2851 at 3199.530 kb on + strand at 3199.531 kb on - strand at 3199.531 kb on - strand at 3199.531 kb on - strand at 3199.531 kb on - strand at 3199.535 kb on + strand at 3199.535 kb on + strand at 3199.535 kb on + strand at 3199.591 kb on + strand at 3199.592 kb on - strand at 3199.598 kb on + strand at 3199.598 kb on + strand at 3199.598 kb on + strand at 3199.598 kb on + strand at 3199.599 kb on - strand at 3199.599 kb on - strand at 3199.599 kb on - strand at 3199.710 kb on + strand, within Pf1N1B4_2852 at 3199.710 kb on + strand, within Pf1N1B4_2852 at 3199.710 kb on + strand, within Pf1N1B4_2852 at 3199.710 kb on + strand, within Pf1N1B4_2852 at 3199.710 kb on + strand, within Pf1N1B4_2852 at 3199.710 kb on + strand at 3199.710 kb on + strand, within Pf1N1B4_2852 at 3199.710 kb on + strand, within Pf1N1B4_2852 at 3199.710 kb on + strand, within Pf1N1B4_2852 at 3199.710 kb on + strand, within Pf1N1B4_2852 at 3199.711 kb on - strand, within Pf1N1B4_2852 at 3199.711 kb on - strand, within Pf1N1B4_2852 at 3199.711 kb on - strand, within Pf1N1B4_2852 at 3199.711 kb on - strand, within Pf1N1B4_2852 at 3199.711 kb on - strand, within Pf1N1B4_2852 at 3199.711 kb on - strand, within Pf1N1B4_2852 at 3199.711 kb on - strand, within Pf1N1B4_2852 at 3199.711 kb on - strand, within Pf1N1B4_2852 at 3199.711 kb on - strand, within Pf1N1B4_2852 at 3199.711 kb on - strand, within Pf1N1B4_2852 at 3199.752 kb on + strand, within Pf1N1B4_2852 at 3199.857 kb on + strand, within Pf1N1B4_2852 at 3199.857 kb on + strand, within Pf1N1B4_2852 at 3199.890 kb on + strand, within Pf1N1B4_2852 at 3199.890 kb on + strand, within Pf1N1B4_2852 at 3199.890 kb on + strand, within Pf1N1B4_2852 at 3199.890 kb on + strand, within Pf1N1B4_2852 at 3199.890 kb on + strand, within Pf1N1B4_2852 at 3199.890 kb on + strand, within Pf1N1B4_2852 at 3199.890 kb on + strand, within Pf1N1B4_2852 at 3199.891 kb on - strand, within Pf1N1B4_2852 at 3199.891 kb on - strand, within Pf1N1B4_2852 at 3199.891 kb on - strand, within Pf1N1B4_2852 at 3199.908 kb on + strand, within Pf1N1B4_2852 at 3199.908 kb on + strand, within Pf1N1B4_2852 at 3199.909 kb on - strand, within Pf1N1B4_2852 at 3199.909 kb on - strand, within Pf1N1B4_2852 at 3199.965 kb on + strand, within Pf1N1B4_2852 at 3199.965 kb on + strand, within Pf1N1B4_2852 at 3200.093 kb on + strand at 3200.093 kb on + strand at 3200.093 kb on + strand at 3200.093 kb on + strand at 3200.093 kb on + strand at 3200.093 kb on + strand at 3200.093 kb on + strand at 3200.094 kb on - strand at 3200.094 kb on - strand at 3200.149 kb on + strand at 3200.149 kb on + strand at 3200.152 kb on + strand at 3200.152 kb on + strand at 3200.153 kb on - strand at 3200.219 kb on + strand at 3200.226 kb on + strand at 3200.226 kb on + strand at 3200.362 kb on - strand, within Pf1N1B4_2853 at 3200.441 kb on - strand, within Pf1N1B4_2853 at 3200.472 kb on + strand, within Pf1N1B4_2853
Per-strain Table
Position Strand Gene LocusTag Fraction Cholin acetate 10 mM remove 3,198,436 - Pf1N1B4_2850 0.41 +0.6 3,198,513 + Pf1N1B4_2850 0.46 +1.6 3,198,513 + Pf1N1B4_2850 0.46 +0.8 3,198,513 + Pf1N1B4_2850 0.46 -1.4 3,198,513 + Pf1N1B4_2850 0.46 +1.1 3,198,513 + Pf1N1B4_2850 0.46 -0.1 3,198,513 + Pf1N1B4_2850 0.46 +2.0 3,198,514 - Pf1N1B4_2850 0.46 -0.0 3,198,595 - Pf1N1B4_2850 0.51 -1.4 3,198,696 + Pf1N1B4_2850 0.57 -0.6 3,198,867 + -0.1 3,198,868 - Pf1N1B4_2850 0.67 +0.1 3,199,049 + Pf1N1B4_2850 0.78 -0.4 3,199,049 + Pf1N1B4_2850 0.78 -0.7 3,199,049 + Pf1N1B4_2850 0.78 +0.1 3,199,049 + Pf1N1B4_2850 0.78 -0.9 3,199,049 + Pf1N1B4_2850 0.78 -0.9 3,199,049 + Pf1N1B4_2850 0.78 -1.2 3,199,049 + Pf1N1B4_2850 0.78 -1.4 3,199,049 + Pf1N1B4_2850 0.78 +0.3 3,199,049 + Pf1N1B4_2850 0.78 -1.6 3,199,050 - Pf1N1B4_2850 0.78 -1.2 3,199,050 - Pf1N1B4_2850 0.78 -0.4 3,199,050 - Pf1N1B4_2850 0.78 +1.1 3,199,050 - Pf1N1B4_2850 0.78 -1.7 3,199,050 - Pf1N1B4_2850 0.78 -1.4 3,199,050 - Pf1N1B4_2850 0.78 +1.6 3,199,252 - +1.1 3,199,291 + +0.4 3,199,291 + -0.9 3,199,410 + -0.4 3,199,410 + +0.7 3,199,410 + -0.9 3,199,411 - +0.6 3,199,411 - -0.4 3,199,436 + Pf1N1B4_2851 0.29 -0.0 3,199,437 - Pf1N1B4_2851 0.30 -1.2 3,199,437 - Pf1N1B4_2851 0.30 -0.6 3,199,461 + Pf1N1B4_2851 0.47 -2.8 3,199,530 + +0.6 3,199,531 - -1.2 3,199,531 - -1.7 3,199,531 - -0.2 3,199,531 - +0.4 3,199,535 + +0.6 3,199,535 + +2.0 3,199,535 + +0.1 3,199,591 + +0.4 3,199,592 - -0.1 3,199,598 + -2.0 3,199,598 + +0.6 3,199,598 + -0.3 3,199,598 + +1.5 3,199,599 - +1.7 3,199,599 - +0.5 3,199,599 - +0.6 3,199,710 + Pf1N1B4_2852 0.16 +0.4 3,199,710 + Pf1N1B4_2852 0.16 -0.2 3,199,710 + Pf1N1B4_2852 0.16 -0.4 3,199,710 + Pf1N1B4_2852 0.16 -0.7 3,199,710 + Pf1N1B4_2852 0.16 +0.1 3,199,710 + +0.3 3,199,710 + Pf1N1B4_2852 0.16 +0.4 3,199,710 + Pf1N1B4_2852 0.16 -1.4 3,199,710 + Pf1N1B4_2852 0.16 +0.8 3,199,710 + Pf1N1B4_2852 0.16 +0.6 3,199,711 - Pf1N1B4_2852 0.16 -2.7 3,199,711 - Pf1N1B4_2852 0.16 +0.2 3,199,711 - Pf1N1B4_2852 0.16 +0.2 3,199,711 - Pf1N1B4_2852 0.16 +1.1 3,199,711 - Pf1N1B4_2852 0.16 -0.9 3,199,711 - Pf1N1B4_2852 0.16 -0.7 3,199,711 - Pf1N1B4_2852 0.16 +0.8 3,199,711 - Pf1N1B4_2852 0.16 +0.4 3,199,711 - Pf1N1B4_2852 0.16 +3.1 3,199,711 - Pf1N1B4_2852 0.16 -1.0 3,199,752 + Pf1N1B4_2852 0.27 +0.5 3,199,857 + Pf1N1B4_2852 0.55 +0.8 3,199,857 + Pf1N1B4_2852 0.55 -1.9 3,199,890 + Pf1N1B4_2852 0.63 -1.9 3,199,890 + Pf1N1B4_2852 0.63 -0.9 3,199,890 + Pf1N1B4_2852 0.63 +1.7 3,199,890 + Pf1N1B4_2852 0.63 -2.0 3,199,890 + Pf1N1B4_2852 0.63 -1.7 3,199,890 + Pf1N1B4_2852 0.63 -2.6 3,199,890 + Pf1N1B4_2852 0.63 +0.1 3,199,891 - Pf1N1B4_2852 0.64 -1.0 3,199,891 - Pf1N1B4_2852 0.64 +0.8 3,199,891 - Pf1N1B4_2852 0.64 -0.0 3,199,908 + Pf1N1B4_2852 0.68 +0.8 3,199,908 + Pf1N1B4_2852 0.68 -0.7 3,199,909 - Pf1N1B4_2852 0.68 +1.6 3,199,909 - Pf1N1B4_2852 0.68 +1.7 3,199,965 + Pf1N1B4_2852 0.83 +1.3 3,199,965 + Pf1N1B4_2852 0.83 -0.3 3,200,093 + -0.0 3,200,093 + +0.4 3,200,093 + -0.1 3,200,093 + -1.7 3,200,093 + -0.2 3,200,093 + +0.1 3,200,093 + +0.1 3,200,094 - +0.8 3,200,094 - -0.8 3,200,149 + +0.5 3,200,149 + +1.3 3,200,152 + +0.4 3,200,152 + -0.0 3,200,153 - +1.1 3,200,219 + +0.6 3,200,226 + +0.4 3,200,226 + -0.3 3,200,362 - Pf1N1B4_2853 0.16 +0.9 3,200,441 - Pf1N1B4_2853 0.25 +0.1 3,200,472 + Pf1N1B4_2853 0.29 +0.4
Or see this region's nucleotide sequence